Effect of Topical Epigallocatechin Gallate on Corneal Neovascularization in Rabbits
PURPOSE:The aim of this study was to evaluate the efficacy of topical application of epigallocatechin gallate (EGCG) for the treatment of corneal neovascularization in a rabbit model. METHODS:Corneal neovascularization was induced in 12 rabbits by placing a black silk suture in the corneal stroma (2...
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| Veröffentlicht in: | Cornea 2014-05, Vol.33 (5), p.527-532 |
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| creator | Koh, Chang Hyun Lee, Hyun Soo Chung, Sung Kun |
| description | PURPOSE:The aim of this study was to evaluate the efficacy of topical application of epigallocatechin gallate (EGCG) for the treatment of corneal neovascularization in a rabbit model.
METHODS:Corneal neovascularization was induced in 12 rabbits by placing a black silk suture in the corneal stroma (24 eyes) for a week. After suturing, 1 randomly chosen eye of the 12 rabbits was treated with topical EGCG at 2 different concentrations0.01% (group 1) and 0.1% (group 2), whereas the contralateral eyes were treated with sterilized balanced salt solution as the control. All eye drops were applied for 2 weeks after suturing. The suture materials were removed from all eyes on day 7. The surface area of corneal neovascularization was measured and analyzed in all eyes on days 7 and 14. On day 14, all eyes were extracted to measure the concentrations of vascular endothelial growth factor (VEGF) messenger RNA and cyclooxygenase-2 (COX-2) protein.
RESULTS:The surface area of induced corneal neovascularization was significantly smaller only in group 2 compared with that of the control group on days 7 and 14 (P < 0.001). The change in surface area of corneal neovascularization after removal of the suture material was not significantly different between all 3 groups. VEGF messenger RNA levels were significantly lower in group 2 than in the control group (P < 0.001). The concentration of COX-2 was significantly lower in group 2 than in the control group (P = 0.043), but no significant difference was observed between group 1 and the control group.
CONCLUSIONS:Topical administration of EGCG effectively inhibits corneal neovascularization in rabbits. This inhibitory effect is probably related to the suppression of VEGF and COX-2 meditated angiogenesis. |
| doi_str_mv | 10.1097/ICO.0000000000000104 |
| format | Article |
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METHODS:Corneal neovascularization was induced in 12 rabbits by placing a black silk suture in the corneal stroma (24 eyes) for a week. After suturing, 1 randomly chosen eye of the 12 rabbits was treated with topical EGCG at 2 different concentrations0.01% (group 1) and 0.1% (group 2), whereas the contralateral eyes were treated with sterilized balanced salt solution as the control. All eye drops were applied for 2 weeks after suturing. The suture materials were removed from all eyes on day 7. The surface area of corneal neovascularization was measured and analyzed in all eyes on days 7 and 14. On day 14, all eyes were extracted to measure the concentrations of vascular endothelial growth factor (VEGF) messenger RNA and cyclooxygenase-2 (COX-2) protein.
RESULTS:The surface area of induced corneal neovascularization was significantly smaller only in group 2 compared with that of the control group on days 7 and 14 (P < 0.001). The change in surface area of corneal neovascularization after removal of the suture material was not significantly different between all 3 groups. VEGF messenger RNA levels were significantly lower in group 2 than in the control group (P < 0.001). The concentration of COX-2 was significantly lower in group 2 than in the control group (P = 0.043), but no significant difference was observed between group 1 and the control group.
CONCLUSIONS:Topical administration of EGCG effectively inhibits corneal neovascularization in rabbits. This inhibitory effect is probably related to the suppression of VEGF and COX-2 meditated angiogenesis.</description><identifier>ISSN: 0277-3740</identifier><identifier>EISSN: 1536-4798</identifier><identifier>DOI: 10.1097/ICO.0000000000000104</identifier><identifier>PMID: 24608256</identifier><language>eng</language><publisher>United States: by Lippincott Williams & Wilkins</publisher><subject>Administration, Topical ; Angiogenesis Inhibitors - administration & dosage ; Angiogenesis Inhibitors - therapeutic use ; Animals ; Antioxidants - administration & dosage ; Antioxidants - therapeutic use ; Blotting, Western ; Catechin - administration & dosage ; Catechin - analogs & derivatives ; Catechin - therapeutic use ; Cornea - drug effects ; Cornea - metabolism ; Cornea - pathology ; Corneal Neovascularization - metabolism ; Corneal Neovascularization - pathology ; Corneal Neovascularization - prevention & control ; Cyclooxygenase 2 - metabolism ; Disease Models, Animal ; Electrophoresis, Polyacrylamide Gel ; Ophthalmic Solutions ; Rabbits ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - metabolism ; Vascular Endothelial Growth Factor A - genetics</subject><ispartof>Cornea, 2014-05, Vol.33 (5), p.527-532</ispartof><rights>2014 by Lippincott Williams & Wilkins.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4224-2efd0ab2f567f3448266e9d48ea80dd33fb916dffe075bc5e0ad535131dfb8fc3</citedby><cites>FETCH-LOGICAL-c4224-2efd0ab2f567f3448266e9d48ea80dd33fb916dffe075bc5e0ad535131dfb8fc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24608256$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Koh, Chang Hyun</creatorcontrib><creatorcontrib>Lee, Hyun Soo</creatorcontrib><creatorcontrib>Chung, Sung Kun</creatorcontrib><title>Effect of Topical Epigallocatechin Gallate on Corneal Neovascularization in Rabbits</title><title>Cornea</title><addtitle>Cornea</addtitle><description>PURPOSE:The aim of this study was to evaluate the efficacy of topical application of epigallocatechin gallate (EGCG) for the treatment of corneal neovascularization in a rabbit model.
METHODS:Corneal neovascularization was induced in 12 rabbits by placing a black silk suture in the corneal stroma (24 eyes) for a week. After suturing, 1 randomly chosen eye of the 12 rabbits was treated with topical EGCG at 2 different concentrations0.01% (group 1) and 0.1% (group 2), whereas the contralateral eyes were treated with sterilized balanced salt solution as the control. All eye drops were applied for 2 weeks after suturing. The suture materials were removed from all eyes on day 7. The surface area of corneal neovascularization was measured and analyzed in all eyes on days 7 and 14. On day 14, all eyes were extracted to measure the concentrations of vascular endothelial growth factor (VEGF) messenger RNA and cyclooxygenase-2 (COX-2) protein.
RESULTS:The surface area of induced corneal neovascularization was significantly smaller only in group 2 compared with that of the control group on days 7 and 14 (P < 0.001). The change in surface area of corneal neovascularization after removal of the suture material was not significantly different between all 3 groups. VEGF messenger RNA levels were significantly lower in group 2 than in the control group (P < 0.001). The concentration of COX-2 was significantly lower in group 2 than in the control group (P = 0.043), but no significant difference was observed between group 1 and the control group.
CONCLUSIONS:Topical administration of EGCG effectively inhibits corneal neovascularization in rabbits. This inhibitory effect is probably related to the suppression of VEGF and COX-2 meditated angiogenesis.</description><subject>Administration, Topical</subject><subject>Angiogenesis Inhibitors - administration & dosage</subject><subject>Angiogenesis Inhibitors - therapeutic use</subject><subject>Animals</subject><subject>Antioxidants - administration & dosage</subject><subject>Antioxidants - therapeutic use</subject><subject>Blotting, Western</subject><subject>Catechin - administration & dosage</subject><subject>Catechin - analogs & derivatives</subject><subject>Catechin - therapeutic use</subject><subject>Cornea - drug effects</subject><subject>Cornea - metabolism</subject><subject>Cornea - pathology</subject><subject>Corneal Neovascularization - metabolism</subject><subject>Corneal Neovascularization - pathology</subject><subject>Corneal Neovascularization - prevention & control</subject><subject>Cyclooxygenase 2 - metabolism</subject><subject>Disease Models, Animal</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Ophthalmic Solutions</subject><subject>Rabbits</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - metabolism</subject><subject>Vascular Endothelial Growth Factor A - genetics</subject><issn>0277-3740</issn><issn>1536-4798</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kF1PwyAUhonRuDn9B8b00ptOoHy0l2aZc8niEp3XhFJwaFcqtC7668VsGuOF3MAJz3nPyQPAOYJjBAt-NZ8sx_D3QZAcgCGiGUsJL_JDMISY8zTjBA7ASQjPkeGc4WMwwITBHFM2BA9TY7TqEmeSlWutknUybe2TrGunZKfV2jbJLFbxnbgmmTjf6Mjcafcmg-pr6e2H7Gz8iuC9LEvbhVNwZGQd9Nn-HoHHm-lqcpsulrP55HqRKoIxSbE2FZQlNpRxkxGSY8Z0UZFcyxxWVZaZskCsivtBTktFNZQVzSjKUGXK3KhsBC53ua13r70OndjYoHRcttGuDwJRhDEuSEEjSnao8i4Er41ovd1I_y4QFF86RdQp_uqMbRf7CX250dVP07e_COQ7YOvqTvvwUvdb7cU6OurW_2d_Ahr1gac</recordid><startdate>201405</startdate><enddate>201405</enddate><creator>Koh, Chang Hyun</creator><creator>Lee, Hyun Soo</creator><creator>Chung, Sung Kun</creator><general>by Lippincott Williams & Wilkins</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201405</creationdate><title>Effect of Topical Epigallocatechin Gallate on Corneal Neovascularization in Rabbits</title><author>Koh, Chang Hyun ; Lee, Hyun Soo ; Chung, Sung Kun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4224-2efd0ab2f567f3448266e9d48ea80dd33fb916dffe075bc5e0ad535131dfb8fc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Administration, Topical</topic><topic>Angiogenesis Inhibitors - administration & dosage</topic><topic>Angiogenesis Inhibitors - therapeutic use</topic><topic>Animals</topic><topic>Antioxidants - administration & dosage</topic><topic>Antioxidants - therapeutic use</topic><topic>Blotting, Western</topic><topic>Catechin - administration & dosage</topic><topic>Catechin - analogs & derivatives</topic><topic>Catechin - therapeutic use</topic><topic>Cornea - drug effects</topic><topic>Cornea - metabolism</topic><topic>Cornea - pathology</topic><topic>Corneal Neovascularization - metabolism</topic><topic>Corneal Neovascularization - pathology</topic><topic>Corneal Neovascularization - prevention & control</topic><topic>Cyclooxygenase 2 - metabolism</topic><topic>Disease Models, Animal</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Ophthalmic Solutions</topic><topic>Rabbits</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - metabolism</topic><topic>Vascular Endothelial Growth Factor A - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Koh, Chang Hyun</creatorcontrib><creatorcontrib>Lee, Hyun Soo</creatorcontrib><creatorcontrib>Chung, Sung Kun</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cornea</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Koh, Chang Hyun</au><au>Lee, Hyun Soo</au><au>Chung, Sung Kun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of Topical Epigallocatechin Gallate on Corneal Neovascularization in Rabbits</atitle><jtitle>Cornea</jtitle><addtitle>Cornea</addtitle><date>2014-05</date><risdate>2014</risdate><volume>33</volume><issue>5</issue><spage>527</spage><epage>532</epage><pages>527-532</pages><issn>0277-3740</issn><eissn>1536-4798</eissn><abstract>PURPOSE:The aim of this study was to evaluate the efficacy of topical application of epigallocatechin gallate (EGCG) for the treatment of corneal neovascularization in a rabbit model.
METHODS:Corneal neovascularization was induced in 12 rabbits by placing a black silk suture in the corneal stroma (24 eyes) for a week. After suturing, 1 randomly chosen eye of the 12 rabbits was treated with topical EGCG at 2 different concentrations0.01% (group 1) and 0.1% (group 2), whereas the contralateral eyes were treated with sterilized balanced salt solution as the control. All eye drops were applied for 2 weeks after suturing. The suture materials were removed from all eyes on day 7. The surface area of corneal neovascularization was measured and analyzed in all eyes on days 7 and 14. On day 14, all eyes were extracted to measure the concentrations of vascular endothelial growth factor (VEGF) messenger RNA and cyclooxygenase-2 (COX-2) protein.
RESULTS:The surface area of induced corneal neovascularization was significantly smaller only in group 2 compared with that of the control group on days 7 and 14 (P < 0.001). The change in surface area of corneal neovascularization after removal of the suture material was not significantly different between all 3 groups. VEGF messenger RNA levels were significantly lower in group 2 than in the control group (P < 0.001). The concentration of COX-2 was significantly lower in group 2 than in the control group (P = 0.043), but no significant difference was observed between group 1 and the control group.
CONCLUSIONS:Topical administration of EGCG effectively inhibits corneal neovascularization in rabbits. This inhibitory effect is probably related to the suppression of VEGF and COX-2 meditated angiogenesis.</abstract><cop>United States</cop><pub>by Lippincott Williams & Wilkins</pub><pmid>24608256</pmid><doi>10.1097/ICO.0000000000000104</doi><tpages>6</tpages></addata></record> |
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| subjects | Administration, Topical Angiogenesis Inhibitors - administration & dosage Angiogenesis Inhibitors - therapeutic use Animals Antioxidants - administration & dosage Antioxidants - therapeutic use Blotting, Western Catechin - administration & dosage Catechin - analogs & derivatives Catechin - therapeutic use Cornea - drug effects Cornea - metabolism Cornea - pathology Corneal Neovascularization - metabolism Corneal Neovascularization - pathology Corneal Neovascularization - prevention & control Cyclooxygenase 2 - metabolism Disease Models, Animal Electrophoresis, Polyacrylamide Gel Ophthalmic Solutions Rabbits Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - metabolism Vascular Endothelial Growth Factor A - genetics |
| title | Effect of Topical Epigallocatechin Gallate on Corneal Neovascularization in Rabbits |
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