Sperm retrieval from patients with nonmosaic Klinefelter's syndrome by semen cytology examination
Successful sperm retrieval from ejaculates of nonmosaic Klinefelter's syndrome (KS) patients by using semen cytology examination was described in this report. The clinical parameters of KS patients with sperm compared to patients without sperm were described. One hundred and fifty-one patients...
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| Veröffentlicht in: | Genetics and molecular research 2014-03, Vol.13 (1), p.1848-1854 |
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| creator | Jiang, Y-T Dong, Y Yu, X-W Du, R-C Li, L-L Zhang, H-G Liu, R-Z |
| description | Successful sperm retrieval from ejaculates of nonmosaic Klinefelter's syndrome (KS) patients by using semen cytology examination was described in this report. The clinical parameters of KS patients with sperm compared to patients without sperm were described. One hundred and fifty-one patients were proven to suffer from KS by chromosomal analysis using G-banding. Spermatozoa were obtained from 10 patients (10/151, 6.6%) using semen analysis. After semen cytology examination, 32 patients (32/151, 21.2%) were found to have sperm or germ cell in their ejaculate. The patients with successful sperm retrieval were significantly younger (27.1 ± 3.7 years) than the patients for whom sperm retrieval failed (28.9 ± 4.2 years). The mean serum testosterone level and the mean T/LH ratio of KS patients with successful sperm retrieval were significantly higher in men with sperm than in men without sperm (testosterone: 3.2 ± 2.1 ng/mL vs 2.7 ± 1.5 ng/mL; T/LH ratio: 0.2 ± 0.3 vs 0.1 ± 0.1). In conclusion, semen cytology examination should be performed to identify sperm and germ cells in the ejaculate of KS patients if no sperm can be detected by traditional semen analysis. The serum testosterone level and T/LH ratio revealed an association between impaired Leydig cell function and impaired spermatogenesis in KS males. KS patients should receive earlier diagnosis and treatment. |
| doi_str_mv | 10.4238/2014.March.17.12 |
| format | Article |
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The clinical parameters of KS patients with sperm compared to patients without sperm were described. One hundred and fifty-one patients were proven to suffer from KS by chromosomal analysis using G-banding. Spermatozoa were obtained from 10 patients (10/151, 6.6%) using semen analysis. After semen cytology examination, 32 patients (32/151, 21.2%) were found to have sperm or germ cell in their ejaculate. The patients with successful sperm retrieval were significantly younger (27.1 ± 3.7 years) than the patients for whom sperm retrieval failed (28.9 ± 4.2 years). The mean serum testosterone level and the mean T/LH ratio of KS patients with successful sperm retrieval were significantly higher in men with sperm than in men without sperm (testosterone: 3.2 ± 2.1 ng/mL vs 2.7 ± 1.5 ng/mL; T/LH ratio: 0.2 ± 0.3 vs 0.1 ± 0.1). In conclusion, semen cytology examination should be performed to identify sperm and germ cells in the ejaculate of KS patients if no sperm can be detected by traditional semen analysis. The serum testosterone level and T/LH ratio revealed an association between impaired Leydig cell function and impaired spermatogenesis in KS males. KS patients should receive earlier diagnosis and treatment.</description><identifier>ISSN: 1676-5680</identifier><identifier>EISSN: 1676-5680</identifier><identifier>DOI: 10.4238/2014.March.17.12</identifier><identifier>PMID: 24668672</identifier><language>eng</language><publisher>Brazil</publisher><subject>Adult ; Azoospermia - genetics ; Humans ; Klinefelter Syndrome - genetics ; Klinefelter Syndrome - pathology ; Male ; Mosaicism ; Semen ; Sperm Retrieval ; Spermatogenesis - genetics ; Testis - growth & development ; Testis - pathology</subject><ispartof>Genetics and molecular research, 2014-03, Vol.13 (1), p.1848-1854</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c341t-702e4bd45693b331a96a3b1ec331910fbe9a615db4dd450950d1976f6a90e1373</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24668672$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jiang, Y-T</creatorcontrib><creatorcontrib>Dong, Y</creatorcontrib><creatorcontrib>Yu, X-W</creatorcontrib><creatorcontrib>Du, R-C</creatorcontrib><creatorcontrib>Li, L-L</creatorcontrib><creatorcontrib>Zhang, H-G</creatorcontrib><creatorcontrib>Liu, R-Z</creatorcontrib><title>Sperm retrieval from patients with nonmosaic Klinefelter's syndrome by semen cytology examination</title><title>Genetics and molecular research</title><addtitle>Genet Mol Res</addtitle><description>Successful sperm retrieval from ejaculates of nonmosaic Klinefelter's syndrome (KS) patients by using semen cytology examination was described in this report. The clinical parameters of KS patients with sperm compared to patients without sperm were described. One hundred and fifty-one patients were proven to suffer from KS by chromosomal analysis using G-banding. Spermatozoa were obtained from 10 patients (10/151, 6.6%) using semen analysis. After semen cytology examination, 32 patients (32/151, 21.2%) were found to have sperm or germ cell in their ejaculate. The patients with successful sperm retrieval were significantly younger (27.1 ± 3.7 years) than the patients for whom sperm retrieval failed (28.9 ± 4.2 years). The mean serum testosterone level and the mean T/LH ratio of KS patients with successful sperm retrieval were significantly higher in men with sperm than in men without sperm (testosterone: 3.2 ± 2.1 ng/mL vs 2.7 ± 1.5 ng/mL; T/LH ratio: 0.2 ± 0.3 vs 0.1 ± 0.1). In conclusion, semen cytology examination should be performed to identify sperm and germ cells in the ejaculate of KS patients if no sperm can be detected by traditional semen analysis. The serum testosterone level and T/LH ratio revealed an association between impaired Leydig cell function and impaired spermatogenesis in KS males. KS patients should receive earlier diagnosis and treatment.</description><subject>Adult</subject><subject>Azoospermia - genetics</subject><subject>Humans</subject><subject>Klinefelter Syndrome - genetics</subject><subject>Klinefelter Syndrome - pathology</subject><subject>Male</subject><subject>Mosaicism</subject><subject>Semen</subject><subject>Sperm Retrieval</subject><subject>Spermatogenesis - genetics</subject><subject>Testis - growth & development</subject><subject>Testis - pathology</subject><issn>1676-5680</issn><issn>1676-5680</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkD1PwzAURS0EoqWwMyFvsCT42Y6TjKjiS4AYgNlykhcalNjFToH8e1JaENO9w7l3OIQcA4slF9k5ZyDjB-PLRQxpDHyHTEGlKkpUxnb_9Qk5COGNMZ7IjO2TCZdKZSrlU2Kelug76rH3DX6YltbedXRp-gZtH-hn0y-odbZzwTQlvWsbizW2PfrTQMNgq5FGWgw0YIeWlkPvWvc6UPwyXWPHF2cPyV5t2oBH25yRl6vL5_lNdP94fTu_uI9KIaGPUsZRFpVMVC4KIcDkyogCsBx7DqwuMDcKkqqQ1QixPGEV5KmqlckZgkjFjJxtfpfeva8w9LprQoltayy6VdCQAEtBAmcjyjZo6V0IHmu99E1n_KCB6bVYvRarf8RqSDXwcXKyfV8VHVZ_g1-T4htUsnX_</recordid><startdate>20140317</startdate><enddate>20140317</enddate><creator>Jiang, Y-T</creator><creator>Dong, Y</creator><creator>Yu, X-W</creator><creator>Du, R-C</creator><creator>Li, L-L</creator><creator>Zhang, H-G</creator><creator>Liu, R-Z</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20140317</creationdate><title>Sperm retrieval from patients with nonmosaic Klinefelter's syndrome by semen cytology examination</title><author>Jiang, Y-T ; Dong, Y ; Yu, X-W ; Du, R-C ; Li, L-L ; Zhang, H-G ; Liu, R-Z</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c341t-702e4bd45693b331a96a3b1ec331910fbe9a615db4dd450950d1976f6a90e1373</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Adult</topic><topic>Azoospermia - genetics</topic><topic>Humans</topic><topic>Klinefelter Syndrome - genetics</topic><topic>Klinefelter Syndrome - pathology</topic><topic>Male</topic><topic>Mosaicism</topic><topic>Semen</topic><topic>Sperm Retrieval</topic><topic>Spermatogenesis - genetics</topic><topic>Testis - growth & development</topic><topic>Testis - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jiang, Y-T</creatorcontrib><creatorcontrib>Dong, Y</creatorcontrib><creatorcontrib>Yu, X-W</creatorcontrib><creatorcontrib>Du, R-C</creatorcontrib><creatorcontrib>Li, L-L</creatorcontrib><creatorcontrib>Zhang, H-G</creatorcontrib><creatorcontrib>Liu, R-Z</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Genetics and molecular research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jiang, Y-T</au><au>Dong, Y</au><au>Yu, X-W</au><au>Du, R-C</au><au>Li, L-L</au><au>Zhang, H-G</au><au>Liu, R-Z</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sperm retrieval from patients with nonmosaic Klinefelter's syndrome by semen cytology examination</atitle><jtitle>Genetics and molecular research</jtitle><addtitle>Genet Mol Res</addtitle><date>2014-03-17</date><risdate>2014</risdate><volume>13</volume><issue>1</issue><spage>1848</spage><epage>1854</epage><pages>1848-1854</pages><issn>1676-5680</issn><eissn>1676-5680</eissn><abstract>Successful sperm retrieval from ejaculates of nonmosaic Klinefelter's syndrome (KS) patients by using semen cytology examination was described in this report. The clinical parameters of KS patients with sperm compared to patients without sperm were described. One hundred and fifty-one patients were proven to suffer from KS by chromosomal analysis using G-banding. Spermatozoa were obtained from 10 patients (10/151, 6.6%) using semen analysis. After semen cytology examination, 32 patients (32/151, 21.2%) were found to have sperm or germ cell in their ejaculate. The patients with successful sperm retrieval were significantly younger (27.1 ± 3.7 years) than the patients for whom sperm retrieval failed (28.9 ± 4.2 years). The mean serum testosterone level and the mean T/LH ratio of KS patients with successful sperm retrieval were significantly higher in men with sperm than in men without sperm (testosterone: 3.2 ± 2.1 ng/mL vs 2.7 ± 1.5 ng/mL; T/LH ratio: 0.2 ± 0.3 vs 0.1 ± 0.1). In conclusion, semen cytology examination should be performed to identify sperm and germ cells in the ejaculate of KS patients if no sperm can be detected by traditional semen analysis. The serum testosterone level and T/LH ratio revealed an association between impaired Leydig cell function and impaired spermatogenesis in KS males. KS patients should receive earlier diagnosis and treatment.</abstract><cop>Brazil</cop><pmid>24668672</pmid><doi>10.4238/2014.March.17.12</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adult Azoospermia - genetics Humans Klinefelter Syndrome - genetics Klinefelter Syndrome - pathology Male Mosaicism Semen Sperm Retrieval Spermatogenesis - genetics Testis - growth & development Testis - pathology |
| title | Sperm retrieval from patients with nonmosaic Klinefelter's syndrome by semen cytology examination |
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