Tumor cell characterization and classification based on cellular specific membrane capacitance and cytoplasm conductivity
This paper reports a microfluidic system that enables the characterization of tumor cell electrical properties where cells were aspirated through a constriction channel (cross-section area smaller than that of biological cells) with cellular impedance profiles measured and translated to specific mem...
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| Veröffentlicht in: | Biosensors & bioelectronics 2014-07, Vol.57, p.245-253 |
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| creator | Zhao, Y. Zhao, X.T. Chen, D.Y. Luo, Y.N. Jiang, M. Wei, C. Long, R. Yue, W.T. Wang, J.B. Chen, J. |
| description | This paper reports a microfluidic system that enables the characterization of tumor cell electrical properties where cells were aspirated through a constriction channel (cross-section area smaller than that of biological cells) with cellular impedance profiles measured and translated to specific membrane capacitance (Cspecific membrane) and cytoplasm conductivity (σcytoplasm). Two batches of H1299 cells were quantified by the microfluidic platform with different constriction channel cross-section areas, recording no differences with statistical significance (p |
| doi_str_mv | 10.1016/j.bios.2014.02.026 |
| format | Article |
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•A microfluidic platform to quantify tumor cell electrical properties was developed.•Electrical differences from paired high and low-metastatic cell lines were observed.•Electrical differences with/without single oncogene regulation were observed.•Correlation between Cspecific membrane and membrane protein expression was located.•Correlation between σcytoplasm and cytosolic protein expression was located.</description><identifier>ISSN: 0956-5663</identifier><identifier>EISSN: 1873-4235</identifier><identifier>DOI: 10.1016/j.bios.2014.02.026</identifier><identifier>PMID: 24594591</identifier><language>eng</language><publisher>Kidlington: Elsevier B.V</publisher><subject>Biological and medical sciences ; Biosensing Techniques - instrumentation ; Biotechnology ; Cell Line, Tumor ; Cell Membrane - chemistry ; Cell Membrane - pathology ; Cytoplasm - chemistry ; Cytoplasm - pathology ; Cytoplasm conductivity ; Electric Capacitance ; Electric Conductivity ; Equipment Design ; Fundamental and applied biological sciences. Psychology ; Humans ; Microfluidic Analytical Techniques - instrumentation ; Microfluidics ; Neoplasm Metastasis - pathology ; Neoplasms - chemistry ; Neoplasms - pathology ; Reproducibility of Results ; Single-cell analysis ; Single-Cell Analysis - instrumentation ; Specific membrane capacitance ; Tumor cell classification</subject><ispartof>Biosensors & bioelectronics, 2014-07, Vol.57, p.245-253</ispartof><rights>2014 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2014 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-a1865ff04e221faf9cf6e60de88bafcaf0870b67e09838244da75c8dab4f87a93</citedby><cites>FETCH-LOGICAL-c386t-a1865ff04e221faf9cf6e60de88bafcaf0870b67e09838244da75c8dab4f87a93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0956566314001043$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=28340459$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24594591$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhao, Y.</creatorcontrib><creatorcontrib>Zhao, X.T.</creatorcontrib><creatorcontrib>Chen, D.Y.</creatorcontrib><creatorcontrib>Luo, Y.N.</creatorcontrib><creatorcontrib>Jiang, M.</creatorcontrib><creatorcontrib>Wei, C.</creatorcontrib><creatorcontrib>Long, R.</creatorcontrib><creatorcontrib>Yue, W.T.</creatorcontrib><creatorcontrib>Wang, J.B.</creatorcontrib><creatorcontrib>Chen, J.</creatorcontrib><title>Tumor cell characterization and classification based on cellular specific membrane capacitance and cytoplasm conductivity</title><title>Biosensors & bioelectronics</title><addtitle>Biosens Bioelectron</addtitle><description>This paper reports a microfluidic system that enables the characterization of tumor cell electrical properties where cells were aspirated through a constriction channel (cross-section area smaller than that of biological cells) with cellular impedance profiles measured and translated to specific membrane capacitance (Cspecific membrane) and cytoplasm conductivity (σcytoplasm). Two batches of H1299 cells were quantified by the microfluidic platform with different constriction channel cross-section areas, recording no differences with statistical significance (p<0.001) in both Cspecific membrane (1.63±0.52 vs. 1.65±0.43μF/cm2) and σcytoplasm (0.90±0.19 vs. 0.92±0.15S/m), and thus confirming the reliability of the microfluidic platform. For paired high- and low-metastatic carcinoma strains 95D (ncell=537) and 95C cells (ncell=486), significant differences in both Cspecific membrane (2.00±0.43 vs. 1.62±0.39μF/cm2) and σcytoplasm (0.88±0.46 vs. 1.25±0.35S/m) were observed. Statistically significant difference only in Cspecific membrane (2.00±0.43 vs. 1.58±0.30μF/cm2) was observed for 95D cells (ncell=537) and 95D CCNY-KD cells with single oncogene CCNY down regulation (ncell=479, CCNY is a membrane-associated protein). In addition, statistically significant difference only in σcytoplasm (0.73±0.17 vs. 1.01±0.17S/m) was observed for A549 cells (ncell=487) and A549 CypA-KD cells with single oncogene CypA down regulation (ncell=597, CypA is a cytosolic protein). These results validated the developed microfluidic platform for Cspecific membrane and σcytoplasm quantification and confirmed the feasibility of using Cspecific membrane and σcytoplasm for tumor cell classification.
•A microfluidic platform to quantify tumor cell electrical properties was developed.•Electrical differences from paired high and low-metastatic cell lines were observed.•Electrical differences with/without single oncogene regulation were observed.•Correlation between Cspecific membrane and membrane protein expression was located.•Correlation between σcytoplasm and cytosolic protein expression was located.</description><subject>Biological and medical sciences</subject><subject>Biosensing Techniques - instrumentation</subject><subject>Biotechnology</subject><subject>Cell Line, Tumor</subject><subject>Cell Membrane - chemistry</subject><subject>Cell Membrane - pathology</subject><subject>Cytoplasm - chemistry</subject><subject>Cytoplasm - pathology</subject><subject>Cytoplasm conductivity</subject><subject>Electric Capacitance</subject><subject>Electric Conductivity</subject><subject>Equipment Design</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Microfluidic Analytical Techniques - instrumentation</subject><subject>Microfluidics</subject><subject>Neoplasm Metastasis - pathology</subject><subject>Neoplasms - chemistry</subject><subject>Neoplasms - pathology</subject><subject>Reproducibility of Results</subject><subject>Single-cell analysis</subject><subject>Single-Cell Analysis - instrumentation</subject><subject>Specific membrane capacitance</subject><subject>Tumor cell classification</subject><issn>0956-5663</issn><issn>1873-4235</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMGK1TAUhoMozp3RF3Ah3Qhuek3aNE3BjQw6CgNuxnU4PTnBXNqmJu3A9eknpVfdCQcSku_8_HyMvRH8KLhQH07H3od0rLiQR17lUc_YQei2LmVVN8_ZgXeNKhul6it2ndKJc96Kjr9kV5VsujziwM4P6xhigTQMBf6ECLhQ9L9h8WEqYLIFDpCSdx73px4S2SJfto11gFikmXD7L0Ya-wgTFQgzoF9gQtojzkuYc8xYYJjsiot_9Mv5FXvhYEj0-nLesB9fPj_cfi3vv999u_10X2Kt1VKC0KpxjkuqKuHAdegUKW5J6x4cguO65b1qiXe61pWUFtoGtYVeOt1CV9-w93vuHMOvldJiRp-29rlqWJMRTZYpZK14RqsdxRhSiuTMHP0I8WwEN5tyczKbcrMpN7zKo_LS20v-2o9k_678cZyBdxcAEsLgsiP06R-na8kzmLmPO0fZxqOnaBJ6yhKtj4SLscH_r8cTjbeizQ</recordid><startdate>20140715</startdate><enddate>20140715</enddate><creator>Zhao, Y.</creator><creator>Zhao, X.T.</creator><creator>Chen, D.Y.</creator><creator>Luo, Y.N.</creator><creator>Jiang, M.</creator><creator>Wei, C.</creator><creator>Long, R.</creator><creator>Yue, W.T.</creator><creator>Wang, J.B.</creator><creator>Chen, J.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20140715</creationdate><title>Tumor cell characterization and classification based on cellular specific membrane capacitance and cytoplasm conductivity</title><author>Zhao, Y. ; Zhao, X.T. ; Chen, D.Y. ; Luo, Y.N. ; Jiang, M. ; Wei, C. ; Long, R. ; Yue, W.T. ; Wang, J.B. ; Chen, J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-a1865ff04e221faf9cf6e60de88bafcaf0870b67e09838244da75c8dab4f87a93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Biological and medical sciences</topic><topic>Biosensing Techniques - instrumentation</topic><topic>Biotechnology</topic><topic>Cell Line, Tumor</topic><topic>Cell Membrane - chemistry</topic><topic>Cell Membrane - pathology</topic><topic>Cytoplasm - chemistry</topic><topic>Cytoplasm - pathology</topic><topic>Cytoplasm conductivity</topic><topic>Electric Capacitance</topic><topic>Electric Conductivity</topic><topic>Equipment Design</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Microfluidic Analytical Techniques - instrumentation</topic><topic>Microfluidics</topic><topic>Neoplasm Metastasis - pathology</topic><topic>Neoplasms - chemistry</topic><topic>Neoplasms - pathology</topic><topic>Reproducibility of Results</topic><topic>Single-cell analysis</topic><topic>Single-Cell Analysis - instrumentation</topic><topic>Specific membrane capacitance</topic><topic>Tumor cell classification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhao, Y.</creatorcontrib><creatorcontrib>Zhao, X.T.</creatorcontrib><creatorcontrib>Chen, D.Y.</creatorcontrib><creatorcontrib>Luo, Y.N.</creatorcontrib><creatorcontrib>Jiang, M.</creatorcontrib><creatorcontrib>Wei, C.</creatorcontrib><creatorcontrib>Long, R.</creatorcontrib><creatorcontrib>Yue, W.T.</creatorcontrib><creatorcontrib>Wang, J.B.</creatorcontrib><creatorcontrib>Chen, J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biosensors & bioelectronics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhao, Y.</au><au>Zhao, X.T.</au><au>Chen, D.Y.</au><au>Luo, Y.N.</au><au>Jiang, M.</au><au>Wei, C.</au><au>Long, R.</au><au>Yue, W.T.</au><au>Wang, J.B.</au><au>Chen, J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Tumor cell characterization and classification based on cellular specific membrane capacitance and cytoplasm conductivity</atitle><jtitle>Biosensors & bioelectronics</jtitle><addtitle>Biosens Bioelectron</addtitle><date>2014-07-15</date><risdate>2014</risdate><volume>57</volume><spage>245</spage><epage>253</epage><pages>245-253</pages><issn>0956-5663</issn><eissn>1873-4235</eissn><abstract>This paper reports a microfluidic system that enables the characterization of tumor cell electrical properties where cells were aspirated through a constriction channel (cross-section area smaller than that of biological cells) with cellular impedance profiles measured and translated to specific membrane capacitance (Cspecific membrane) and cytoplasm conductivity (σcytoplasm). Two batches of H1299 cells were quantified by the microfluidic platform with different constriction channel cross-section areas, recording no differences with statistical significance (p<0.001) in both Cspecific membrane (1.63±0.52 vs. 1.65±0.43μF/cm2) and σcytoplasm (0.90±0.19 vs. 0.92±0.15S/m), and thus confirming the reliability of the microfluidic platform. For paired high- and low-metastatic carcinoma strains 95D (ncell=537) and 95C cells (ncell=486), significant differences in both Cspecific membrane (2.00±0.43 vs. 1.62±0.39μF/cm2) and σcytoplasm (0.88±0.46 vs. 1.25±0.35S/m) were observed. Statistically significant difference only in Cspecific membrane (2.00±0.43 vs. 1.58±0.30μF/cm2) was observed for 95D cells (ncell=537) and 95D CCNY-KD cells with single oncogene CCNY down regulation (ncell=479, CCNY is a membrane-associated protein). In addition, statistically significant difference only in σcytoplasm (0.73±0.17 vs. 1.01±0.17S/m) was observed for A549 cells (ncell=487) and A549 CypA-KD cells with single oncogene CypA down regulation (ncell=597, CypA is a cytosolic protein). These results validated the developed microfluidic platform for Cspecific membrane and σcytoplasm quantification and confirmed the feasibility of using Cspecific membrane and σcytoplasm for tumor cell classification.
•A microfluidic platform to quantify tumor cell electrical properties was developed.•Electrical differences from paired high and low-metastatic cell lines were observed.•Electrical differences with/without single oncogene regulation were observed.•Correlation between Cspecific membrane and membrane protein expression was located.•Correlation between σcytoplasm and cytosolic protein expression was located.</abstract><cop>Kidlington</cop><pub>Elsevier B.V</pub><pmid>24594591</pmid><doi>10.1016/j.bios.2014.02.026</doi><tpages>9</tpages></addata></record> |
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| subjects | Biological and medical sciences Biosensing Techniques - instrumentation Biotechnology Cell Line, Tumor Cell Membrane - chemistry Cell Membrane - pathology Cytoplasm - chemistry Cytoplasm - pathology Cytoplasm conductivity Electric Capacitance Electric Conductivity Equipment Design Fundamental and applied biological sciences. Psychology Humans Microfluidic Analytical Techniques - instrumentation Microfluidics Neoplasm Metastasis - pathology Neoplasms - chemistry Neoplasms - pathology Reproducibility of Results Single-cell analysis Single-Cell Analysis - instrumentation Specific membrane capacitance Tumor cell classification |
| title | Tumor cell characterization and classification based on cellular specific membrane capacitance and cytoplasm conductivity |
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