Nanosecond pulsed electric field (nsPEF) treatment for hepatocellular carcinoma: A novel locoregional ablation decreasing lung metastasis
Abstract Hepatocellular carcinoma (HCC) is a highly aggressive malignancy. Nanosecond pulsed electric field (nsPEF) is a new technology destroying tumor cells with a non-thermal high voltage electric field using ultra-short pulses. The study’s aim was to evaluate the ablation efficacy of nsPEFs with...
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| Veröffentlicht in: | Cancer letters 2014-05, Vol.346 (2), p.285-291 |
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| creator | Yin, Shengyong Chen, Xinhua Hu, Chen Zhang, Xueming Hu, Zhenhua Yu, Jun Feng, Xiaowen Jiang, Kai Ye, Shuming Shen, Kezhen Xie, Haiyang Zhou, Lin James Swanson, Robert Zheng, Shusen |
| description | Abstract Hepatocellular carcinoma (HCC) is a highly aggressive malignancy. Nanosecond pulsed electric field (nsPEF) is a new technology destroying tumor cells with a non-thermal high voltage electric field using ultra-short pulses. The study’s aim was to evaluate the ablation efficacy of nsPEFs with human HCC cell lines and a highly metastatic potential HCC xenograft model on BALB/c nude mice. The in vivo study showed nsPEFs induced HCC cell death in a dose dependent manner. On the high metastatic hepatocellular carcinoma cell line (HCCLM3) xenograft mice model, tumor growth was inhibited significantly in nsPEF-treated- groups (single dose and multi-fractionated dose). Besides a local effect, the nsPEF treatment reduced pulmonary metastases. The nsPEFs also enhanced HCC cell phagocytosis by human macrophage cell (THP1) in vitro . The nsPEF is efficient in controlling HCC progression and reducing its metastasis. NsPEF treatment may elicit a host immune response against tumor cells. This study suggests nsPEF therapy could be used as a potential locoregional therapy for hepatocellular carcinoma. |
| doi_str_mv | 10.1016/j.canlet.2014.01.009 |
| format | Article |
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Nanosecond pulsed electric field (nsPEF) is a new technology destroying tumor cells with a non-thermal high voltage electric field using ultra-short pulses. The study’s aim was to evaluate the ablation efficacy of nsPEFs with human HCC cell lines and a highly metastatic potential HCC xenograft model on BALB/c nude mice. The in vivo study showed nsPEFs induced HCC cell death in a dose dependent manner. On the high metastatic hepatocellular carcinoma cell line (HCCLM3) xenograft mice model, tumor growth was inhibited significantly in nsPEF-treated- groups (single dose and multi-fractionated dose). Besides a local effect, the nsPEF treatment reduced pulmonary metastases. The nsPEFs also enhanced HCC cell phagocytosis by human macrophage cell (THP1) in vitro . The nsPEF is efficient in controlling HCC progression and reducing its metastasis. NsPEF treatment may elicit a host immune response against tumor cells. This study suggests nsPEF therapy could be used as a potential locoregional therapy for hepatocellular carcinoma.</description><identifier>ISSN: 0304-3835</identifier><identifier>EISSN: 1872-7980</identifier><identifier>DOI: 10.1016/j.canlet.2014.01.009</identifier><identifier>PMID: 24462824</identifier><language>eng</language><publisher>Ireland: Elsevier Ireland Ltd</publisher><subject>Ablation ; Animals ; Apoptosis ; Cancer therapies ; Carcinoma, Hepatocellular - immunology ; Carcinoma, Hepatocellular - pathology ; Carcinoma, Hepatocellular - therapy ; Catheter Ablation - instrumentation ; Catheter Ablation - methods ; Cell Line, Tumor ; Electric fields ; Electric properties ; Electrodes ; Hematology, Oncology and Palliative Medicine ; Hepatocellular carcinoma ; Humans ; Laboratory animals ; Liver cancer ; Liver Neoplasms - immunology ; Liver Neoplasms - pathology ; Liver Neoplasms - therapy ; Lung Neoplasms - immunology ; Lung Neoplasms - prevention & control ; Lung Neoplasms - secondary ; Lung Neoplasms - therapy ; Macrophages - immunology ; Medical treatment ; Metastasis ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Nanosecond pulsed electric field ; Permeability ; Phagocytosis ; Random Allocation ; Science ; Tumors ; Xenograft Model Antitumor Assays</subject><ispartof>Cancer letters, 2014-05, Vol.346 (2), p.285-291</ispartof><rights>The Authors</rights><rights>2014 The Authors</rights><rights>Copyright © 2014 The Authors. Published by Elsevier Ireland Ltd.. All rights reserved.</rights><rights>Copyright Elsevier Limited May 1, 2014</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c557t-a312ee1261a3b0bb6ba030871af5526c5d3d32513ed16fae381fa604b33911693</citedby><cites>FETCH-LOGICAL-c557t-a312ee1261a3b0bb6ba030871af5526c5d3d32513ed16fae381fa604b33911693</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0304383514000354$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24462824$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yin, Shengyong</creatorcontrib><creatorcontrib>Chen, Xinhua</creatorcontrib><creatorcontrib>Hu, Chen</creatorcontrib><creatorcontrib>Zhang, Xueming</creatorcontrib><creatorcontrib>Hu, Zhenhua</creatorcontrib><creatorcontrib>Yu, Jun</creatorcontrib><creatorcontrib>Feng, Xiaowen</creatorcontrib><creatorcontrib>Jiang, Kai</creatorcontrib><creatorcontrib>Ye, Shuming</creatorcontrib><creatorcontrib>Shen, Kezhen</creatorcontrib><creatorcontrib>Xie, Haiyang</creatorcontrib><creatorcontrib>Zhou, Lin</creatorcontrib><creatorcontrib>James Swanson, Robert</creatorcontrib><creatorcontrib>Zheng, Shusen</creatorcontrib><title>Nanosecond pulsed electric field (nsPEF) treatment for hepatocellular carcinoma: A novel locoregional ablation decreasing lung metastasis</title><title>Cancer letters</title><addtitle>Cancer Lett</addtitle><description>Abstract Hepatocellular carcinoma (HCC) is a highly aggressive malignancy. Nanosecond pulsed electric field (nsPEF) is a new technology destroying tumor cells with a non-thermal high voltage electric field using ultra-short pulses. The study’s aim was to evaluate the ablation efficacy of nsPEFs with human HCC cell lines and a highly metastatic potential HCC xenograft model on BALB/c nude mice. The in vivo study showed nsPEFs induced HCC cell death in a dose dependent manner. On the high metastatic hepatocellular carcinoma cell line (HCCLM3) xenograft mice model, tumor growth was inhibited significantly in nsPEF-treated- groups (single dose and multi-fractionated dose). Besides a local effect, the nsPEF treatment reduced pulmonary metastases. The nsPEFs also enhanced HCC cell phagocytosis by human macrophage cell (THP1) in vitro . The nsPEF is efficient in controlling HCC progression and reducing its metastasis. NsPEF treatment may elicit a host immune response against tumor cells. This study suggests nsPEF therapy could be used as a potential locoregional therapy for hepatocellular carcinoma.</description><subject>Ablation</subject><subject>Animals</subject><subject>Apoptosis</subject><subject>Cancer therapies</subject><subject>Carcinoma, Hepatocellular - immunology</subject><subject>Carcinoma, Hepatocellular - pathology</subject><subject>Carcinoma, Hepatocellular - therapy</subject><subject>Catheter Ablation - instrumentation</subject><subject>Catheter Ablation - methods</subject><subject>Cell Line, Tumor</subject><subject>Electric fields</subject><subject>Electric properties</subject><subject>Electrodes</subject><subject>Hematology, Oncology and Palliative Medicine</subject><subject>Hepatocellular carcinoma</subject><subject>Humans</subject><subject>Laboratory animals</subject><subject>Liver cancer</subject><subject>Liver Neoplasms - immunology</subject><subject>Liver Neoplasms - pathology</subject><subject>Liver Neoplasms - therapy</subject><subject>Lung Neoplasms - immunology</subject><subject>Lung Neoplasms - prevention & control</subject><subject>Lung Neoplasms - secondary</subject><subject>Lung Neoplasms - therapy</subject><subject>Macrophages - immunology</subject><subject>Medical treatment</subject><subject>Metastasis</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Nude</subject><subject>Nanosecond pulsed electric field</subject><subject>Permeability</subject><subject>Phagocytosis</subject><subject>Random Allocation</subject><subject>Science</subject><subject>Tumors</subject><subject>Xenograft Model Antitumor Assays</subject><issn>0304-3835</issn><issn>1872-7980</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFklFr1TAUx4so7m76DUQCvsyH1pwmaXt9EMbYVBgqqM_hND2duabJNWkH-wh-66XcqbAXISR5-J1_8j__UxQvgFfAoXmzqwx6R3NVc5AVh4rz7aNiA11bl-2244-LDRdclqIT6qg4TmnHOVeyVU-Lo1rKpu5quSl-f0IfEpngB7ZfXKKBkSMzR2vYaMkN7NSnLxeXr9kcCeeJ_MzGENkP2uMcDDm3OIzMYDTWhwnfsjPmww055oIJka5t8OgY9g7nfGUDmayTrL9mbsnbRDOmvGx6VjwZMX_g-f15Uny_vPh2_qG8-vz-4_nZVWmUaucSBdREUDeAoud93_SYbXYt4KhU3Rg1iEHUCgQN0IxIooMRGy57IbYAzVacFKcH3X0MvxZKs55sWo2gp7AkDSq3lwsAkdFXD9BdWGL2s1KqbXgnZJcpeaBMDClFGvU-2gnjrQau16j0Th-i0mtUmoPOUeWyl_fiSz_R8LfoTzYZeHcAKHfjxlLUyVjyhgYbc0J6CPZ_LzwUMM56a9D9pFtK_7zoVGuuv67jsk4LyDwpQklxB-ILvGQ</recordid><startdate>20140501</startdate><enddate>20140501</enddate><creator>Yin, Shengyong</creator><creator>Chen, Xinhua</creator><creator>Hu, Chen</creator><creator>Zhang, Xueming</creator><creator>Hu, Zhenhua</creator><creator>Yu, Jun</creator><creator>Feng, Xiaowen</creator><creator>Jiang, Kai</creator><creator>Ye, Shuming</creator><creator>Shen, Kezhen</creator><creator>Xie, Haiyang</creator><creator>Zhou, Lin</creator><creator>James Swanson, Robert</creator><creator>Zheng, Shusen</creator><general>Elsevier Ireland Ltd</general><general>Elsevier Limited</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TO</scope><scope>7U9</scope><scope>H94</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>7X8</scope></search><sort><creationdate>20140501</creationdate><title>Nanosecond pulsed electric field (nsPEF) treatment for hepatocellular carcinoma: A novel locoregional ablation decreasing lung metastasis</title><author>Yin, Shengyong ; Chen, Xinhua ; Hu, Chen ; Zhang, Xueming ; Hu, Zhenhua ; Yu, Jun ; Feng, Xiaowen ; Jiang, Kai ; Ye, Shuming ; Shen, Kezhen ; Xie, Haiyang ; Zhou, Lin ; James Swanson, Robert ; Zheng, Shusen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c557t-a312ee1261a3b0bb6ba030871af5526c5d3d32513ed16fae381fa604b33911693</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Ablation</topic><topic>Animals</topic><topic>Apoptosis</topic><topic>Cancer therapies</topic><topic>Carcinoma, Hepatocellular - immunology</topic><topic>Carcinoma, Hepatocellular - pathology</topic><topic>Carcinoma, Hepatocellular - therapy</topic><topic>Catheter Ablation - instrumentation</topic><topic>Catheter Ablation - methods</topic><topic>Cell Line, Tumor</topic><topic>Electric fields</topic><topic>Electric properties</topic><topic>Electrodes</topic><topic>Hematology, Oncology and Palliative Medicine</topic><topic>Hepatocellular carcinoma</topic><topic>Humans</topic><topic>Laboratory animals</topic><topic>Liver cancer</topic><topic>Liver Neoplasms - immunology</topic><topic>Liver Neoplasms - pathology</topic><topic>Liver Neoplasms - therapy</topic><topic>Lung Neoplasms - immunology</topic><topic>Lung Neoplasms - prevention & control</topic><topic>Lung Neoplasms - secondary</topic><topic>Lung Neoplasms - therapy</topic><topic>Macrophages - immunology</topic><topic>Medical treatment</topic><topic>Metastasis</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mice, Nude</topic><topic>Nanosecond pulsed electric field</topic><topic>Permeability</topic><topic>Phagocytosis</topic><topic>Random Allocation</topic><topic>Science</topic><topic>Tumors</topic><topic>Xenograft Model Antitumor Assays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yin, Shengyong</creatorcontrib><creatorcontrib>Chen, Xinhua</creatorcontrib><creatorcontrib>Hu, Chen</creatorcontrib><creatorcontrib>Zhang, Xueming</creatorcontrib><creatorcontrib>Hu, Zhenhua</creatorcontrib><creatorcontrib>Yu, Jun</creatorcontrib><creatorcontrib>Feng, Xiaowen</creatorcontrib><creatorcontrib>Jiang, Kai</creatorcontrib><creatorcontrib>Ye, Shuming</creatorcontrib><creatorcontrib>Shen, Kezhen</creatorcontrib><creatorcontrib>Xie, Haiyang</creatorcontrib><creatorcontrib>Zhou, Lin</creatorcontrib><creatorcontrib>James Swanson, Robert</creatorcontrib><creatorcontrib>Zheng, Shusen</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>MEDLINE - Academic</collection><jtitle>Cancer letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yin, Shengyong</au><au>Chen, Xinhua</au><au>Hu, Chen</au><au>Zhang, Xueming</au><au>Hu, Zhenhua</au><au>Yu, Jun</au><au>Feng, Xiaowen</au><au>Jiang, Kai</au><au>Ye, Shuming</au><au>Shen, Kezhen</au><au>Xie, Haiyang</au><au>Zhou, Lin</au><au>James Swanson, Robert</au><au>Zheng, Shusen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nanosecond pulsed electric field (nsPEF) treatment for hepatocellular carcinoma: A novel locoregional ablation decreasing lung metastasis</atitle><jtitle>Cancer letters</jtitle><addtitle>Cancer Lett</addtitle><date>2014-05-01</date><risdate>2014</risdate><volume>346</volume><issue>2</issue><spage>285</spage><epage>291</epage><pages>285-291</pages><issn>0304-3835</issn><eissn>1872-7980</eissn><abstract>Abstract Hepatocellular carcinoma (HCC) is a highly aggressive malignancy. Nanosecond pulsed electric field (nsPEF) is a new technology destroying tumor cells with a non-thermal high voltage electric field using ultra-short pulses. The study’s aim was to evaluate the ablation efficacy of nsPEFs with human HCC cell lines and a highly metastatic potential HCC xenograft model on BALB/c nude mice. The in vivo study showed nsPEFs induced HCC cell death in a dose dependent manner. On the high metastatic hepatocellular carcinoma cell line (HCCLM3) xenograft mice model, tumor growth was inhibited significantly in nsPEF-treated- groups (single dose and multi-fractionated dose). Besides a local effect, the nsPEF treatment reduced pulmonary metastases. The nsPEFs also enhanced HCC cell phagocytosis by human macrophage cell (THP1) in vitro . The nsPEF is efficient in controlling HCC progression and reducing its metastasis. NsPEF treatment may elicit a host immune response against tumor cells. This study suggests nsPEF therapy could be used as a potential locoregional therapy for hepatocellular carcinoma.</abstract><cop>Ireland</cop><pub>Elsevier Ireland Ltd</pub><pmid>24462824</pmid><doi>10.1016/j.canlet.2014.01.009</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Ablation Animals Apoptosis Cancer therapies Carcinoma, Hepatocellular - immunology Carcinoma, Hepatocellular - pathology Carcinoma, Hepatocellular - therapy Catheter Ablation - instrumentation Catheter Ablation - methods Cell Line, Tumor Electric fields Electric properties Electrodes Hematology, Oncology and Palliative Medicine Hepatocellular carcinoma Humans Laboratory animals Liver cancer Liver Neoplasms - immunology Liver Neoplasms - pathology Liver Neoplasms - therapy Lung Neoplasms - immunology Lung Neoplasms - prevention & control Lung Neoplasms - secondary Lung Neoplasms - therapy Macrophages - immunology Medical treatment Metastasis Mice Mice, Inbred BALB C Mice, Nude Nanosecond pulsed electric field Permeability Phagocytosis Random Allocation Science Tumors Xenograft Model Antitumor Assays |
| title | Nanosecond pulsed electric field (nsPEF) treatment for hepatocellular carcinoma: A novel locoregional ablation decreasing lung metastasis |
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