Characterization of two active site mutations of thioredoxin reductase from Escherichia coli

Thioredoxin reductase (TRR), a member of the pyridine nucleotide-disulfide oxidoreductase family of flavoenzymes, undergoes two sequential thiol-disulfide interchange reactions with thioredoxin during catalysis. In order to assess the catalytic role of each nascent thiol of the active site disulfide...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	The Journal of biological chemistry 1989-02, Vol.264 (5), p.2656-2664
Hauptverfasser:	Prongay, A J, Engelke, D R, Williams, C H
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Analytical, structural and metabolic biochemistry Binding Sites Biological and medical sciences Biotechnology Cloning, Molecular Enzymes and enzyme inhibitors Escherichia coli Escherichia coli - enzymology Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Genetic technics Kinetics Methods. Procedures. Technologies Molecular Sequence Data Mutant screening Mutation NADH, NADPH Oxidoreductases - metabolism Oxidoreductases Plasmids Prokaryotes Spectrometry, Fluorescence Spectrophotometry thioredoxin Thioredoxin-Disulfide Reductase - genetics Thioredoxin-Disulfide Reductase - metabolism
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!