Construction of new shuttle plasmid vectors for Escherichia coli‐Bacteroides transgeneric cloning

An Escherichia coli‐Bacteroides shuttle vehicle (pKBF367‐1) was constructed by combining the pBR322 derivative pKC7 (5.9 kb) with [1] a 4.6 kb cryptic plasmid from Bacteroides fragilis; and [2] the 4.2 kb EcoRI‐B fragment of the B. fragilis plasmid pBFTM10. This latter component allowed selection of...

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Veröffentlicht in:	FEMS microbiology letters 1988-09, Vol.55 (1), p.15-22
Hauptverfasser:	Pheulpin, Patrice, Tierny, Yann, Béchet, Max, Guillaume, Jean‐Bernard
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteriology Bacteroides Bifunctional vectors Biological and medical sciences Biotechnology Clindamycin resistance cloning vectors Conjugal mobilization Escherichia coli Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics Genetics Methods. Procedures. Technologies Microbiology Shuttle plasmid Transposon Tn501 Vectors (cloning, transfer, expression). Insertion sequences and transposons
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