Multicenter evaluation of the Sepsityper™ extraction kit and MALDI‐TOF MS for direct identification of positive blood culture isolates using the BD BACTEC™ FX and VersaTREK® diagnostic blood culture systems
AIMS: (i) Evaluation of delayed time to blood culture extraction by the Sepsityper kit and impact of shipping pellets off‐site for MALDI‐TOF MS analysis. (ii) Comparison of Sepsityper and laboratory‐developed extraction methods from a literature review. METHODS AND RESULTS: Using two blood culture s...
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| Veröffentlicht in: | Journal of applied microbiology 2014-04, Vol.116 (4), p.934-941 |
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| container_title | Journal of applied microbiology |
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| creator | Schieffer, K.M Tan, K.E Stamper, P.D Somogyi, A Andrea, S.B Wakefield, T Romagnoli, M Chapin, K.C Wolk, D.M Carroll, K.C |
| description | AIMS: (i) Evaluation of delayed time to blood culture extraction by the Sepsityper kit and impact of shipping pellets off‐site for MALDI‐TOF MS analysis. (ii) Comparison of Sepsityper and laboratory‐developed extraction methods from a literature review. METHODS AND RESULTS: Using two blood culture systems (BD BACTEC and VersaTREK), we extracted 411 positive blood cultures using the Sepsityper kit to mimic a potential protocol for institutions without a MALDI‐TOF MS. Extracted pellets were shipped and analysed on the Bruker UltraflexIII. Successful extraction of 358 (87·1%) samples was determined by the presence of detectable proteins. MALDI‐TOF MS correctly identified 332 (80·8%) samples. CONCLUSIONS: Delayed time to extraction did not affect Sepsityper extraction or MALDI‐TOF MS accuracy. The extracted pellets remain stable and provide accurate results by MALDI‐TOF MS when shipped at room temperature to off‐site reference laboratories. SIGNIFICANCE AND IMPACT OF THE STUDY: This is the first study to show that institutions without a MALDI‐TOF MS can take advantage of this innovative technology by shipping a volume of blood to an off‐site laboratory for extraction and MALDI‐TOF MS analysis. We also performed a literature review to compare various extraction methods. |
| doi_str_mv | 10.1111/jam.12434 |
| format | Article |
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(ii) Comparison of Sepsityper and laboratory‐developed extraction methods from a literature review. METHODS AND RESULTS: Using two blood culture systems (BD BACTEC and VersaTREK), we extracted 411 positive blood cultures using the Sepsityper kit to mimic a potential protocol for institutions without a MALDI‐TOF MS. Extracted pellets were shipped and analysed on the Bruker UltraflexIII. Successful extraction of 358 (87·1%) samples was determined by the presence of detectable proteins. MALDI‐TOF MS correctly identified 332 (80·8%) samples. CONCLUSIONS: Delayed time to extraction did not affect Sepsityper extraction or MALDI‐TOF MS accuracy. The extracted pellets remain stable and provide accurate results by MALDI‐TOF MS when shipped at room temperature to off‐site reference laboratories. SIGNIFICANCE AND IMPACT OF THE STUDY: This is the first study to show that institutions without a MALDI‐TOF MS can take advantage of this innovative technology by shipping a volume of blood to an off‐site laboratory for extraction and MALDI‐TOF MS analysis. We also performed a literature review to compare various extraction methods.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1111/jam.12434</identifier><identifier>PMID: 24410849</identifier><identifier>CODEN: JAMIFK</identifier><language>eng</language><publisher>Oxford: Blackwell Science</publisher><subject>bacteraemia ; Bacteremia - diagnosis ; Bacteria - isolation & purification ; Bacterial Proteins - analysis ; Bacterial Proteins - isolation & purification ; Bacteriological Techniques - methods ; Biological and medical sciences ; Biotechnology ; Blood ; blood cultures ; blood volume ; Fundamental and applied biological sciences. Psychology ; Humans ; MALDI‐TOF MS ; matrix-assisted laser desorption-ionization mass spectrometry ; Microbiology ; pellets ; proteins ; sepsis ; Sepsityper ; shipping ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods ; technology ; temperature</subject><ispartof>Journal of applied microbiology, 2014-04, Vol.116 (4), p.934-941</ispartof><rights>2014 The Society for Applied Microbiology</rights><rights>2015 INIST-CNRS</rights><rights>2014 The Society for Applied Microbiology.</rights><rights>Copyright © 2014 The Society for Applied Microbiology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4074-695fc5a07300a5c5f86ed3c2121513c4389db98b07978f4de14240e7bbe91d03</citedby><cites>FETCH-LOGICAL-c4074-695fc5a07300a5c5f86ed3c2121513c4389db98b07978f4de14240e7bbe91d03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjam.12434$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjam.12434$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,777,781,1412,27905,27906,45555,45556</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=28283566$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24410849$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Schieffer, K.M</creatorcontrib><creatorcontrib>Tan, K.E</creatorcontrib><creatorcontrib>Stamper, P.D</creatorcontrib><creatorcontrib>Somogyi, A</creatorcontrib><creatorcontrib>Andrea, S.B</creatorcontrib><creatorcontrib>Wakefield, T</creatorcontrib><creatorcontrib>Romagnoli, M</creatorcontrib><creatorcontrib>Chapin, K.C</creatorcontrib><creatorcontrib>Wolk, D.M</creatorcontrib><creatorcontrib>Carroll, K.C</creatorcontrib><title>Multicenter evaluation of the Sepsityper™ extraction kit and MALDI‐TOF MS for direct identification of positive blood culture isolates using the BD BACTEC™ FX and VersaTREK® diagnostic blood culture systems</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>AIMS: (i) Evaluation of delayed time to blood culture extraction by the Sepsityper kit and impact of shipping pellets off‐site for MALDI‐TOF MS analysis. (ii) Comparison of Sepsityper and laboratory‐developed extraction methods from a literature review. METHODS AND RESULTS: Using two blood culture systems (BD BACTEC and VersaTREK), we extracted 411 positive blood cultures using the Sepsityper kit to mimic a potential protocol for institutions without a MALDI‐TOF MS. Extracted pellets were shipped and analysed on the Bruker UltraflexIII. Successful extraction of 358 (87·1%) samples was determined by the presence of detectable proteins. MALDI‐TOF MS correctly identified 332 (80·8%) samples. CONCLUSIONS: Delayed time to extraction did not affect Sepsityper extraction or MALDI‐TOF MS accuracy. The extracted pellets remain stable and provide accurate results by MALDI‐TOF MS when shipped at room temperature to off‐site reference laboratories. SIGNIFICANCE AND IMPACT OF THE STUDY: This is the first study to show that institutions without a MALDI‐TOF MS can take advantage of this innovative technology by shipping a volume of blood to an off‐site laboratory for extraction and MALDI‐TOF MS analysis. We also performed a literature review to compare various extraction methods.</description><subject>bacteraemia</subject><subject>Bacteremia - diagnosis</subject><subject>Bacteria - isolation & purification</subject><subject>Bacterial Proteins - analysis</subject><subject>Bacterial Proteins - isolation & purification</subject><subject>Bacteriological Techniques - methods</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Blood</subject><subject>blood cultures</subject><subject>blood volume</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>MALDI‐TOF MS</subject><subject>matrix-assisted laser desorption-ionization mass spectrometry</subject><subject>Microbiology</subject><subject>pellets</subject><subject>proteins</subject><subject>sepsis</subject><subject>Sepsityper</subject><subject>shipping</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods</subject><subject>technology</subject><subject>temperature</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kU1uEzEYhkcIREthwQXAEkKCRVr_jmeWaZpAIVElGhC7kcfzTXA6GQfbU8iOBQfgDByAQ3AHLtCT4Py0SJXwxpb8-Hk_602SxwQfkriO5mpxSChn_E6yT1gqejSV9O7mzHsCS7qXPPB-jjFhWKT3kz3KOcEZz_eTP5OuCUZDG8AhuFRNp4KxLbI1Cp8AncPSm7Bagrv6_hPB1-CU3txfmIBUW6FJf3xyevXtx_RshCbnqLYOVcaBDshUUWpqo2-ESxtd5hJQ2VhbIR2TOwfIeNuoAB513rSzTezxCTruD6bDwTp19HGT9AGcV9N3w7e_f8UINWutj4PfcvmVD7DwD5N7tWo8PNrtB8l0NJwOXvfGZ69OB_1xT3MseS_NRa2FwpJhrIQWdZZCxTQllAjCNGdZXpV5VmKZy6zmFRBOOQZZlpCTCrOD5MVWu3T2cwc-FAvjNTSNasF2viACZ6nMCWYRfXYLndvOtXG4NSWlxILTSL3cUtpZ7x3UxdKZhXKrguBiXXURqy42VUf2yc7YlQuobsjrbiPwfAcor1VTO9Vq4_9xGc2YSNPIHW25L6aB1f8Tizf9yXX00-2LWtlCzVy0vj-nOH4EEy5TwtlfueDNug</recordid><startdate>201404</startdate><enddate>201404</enddate><creator>Schieffer, K.M</creator><creator>Tan, K.E</creator><creator>Stamper, P.D</creator><creator>Somogyi, A</creator><creator>Andrea, S.B</creator><creator>Wakefield, T</creator><creator>Romagnoli, M</creator><creator>Chapin, K.C</creator><creator>Wolk, D.M</creator><creator>Carroll, K.C</creator><general>Blackwell Science</general><general>Blackwell</general><general>Oxford University Press</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>201404</creationdate><title>Multicenter evaluation of the Sepsityper™ extraction kit and MALDI‐TOF MS for direct identification of positive blood culture isolates using the BD BACTEC™ FX and VersaTREK® diagnostic blood culture systems</title><author>Schieffer, K.M ; Tan, K.E ; Stamper, P.D ; Somogyi, A ; Andrea, S.B ; Wakefield, T ; Romagnoli, M ; Chapin, K.C ; Wolk, D.M ; Carroll, K.C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4074-695fc5a07300a5c5f86ed3c2121513c4389db98b07978f4de14240e7bbe91d03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>bacteraemia</topic><topic>Bacteremia - diagnosis</topic><topic>Bacteria - isolation & purification</topic><topic>Bacterial Proteins - analysis</topic><topic>Bacterial Proteins - isolation & purification</topic><topic>Bacteriological Techniques - methods</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Blood</topic><topic>blood cultures</topic><topic>blood volume</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>MALDI‐TOF MS</topic><topic>matrix-assisted laser desorption-ionization mass spectrometry</topic><topic>Microbiology</topic><topic>pellets</topic><topic>proteins</topic><topic>sepsis</topic><topic>Sepsityper</topic><topic>shipping</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods</topic><topic>technology</topic><topic>temperature</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schieffer, K.M</creatorcontrib><creatorcontrib>Tan, K.E</creatorcontrib><creatorcontrib>Stamper, P.D</creatorcontrib><creatorcontrib>Somogyi, A</creatorcontrib><creatorcontrib>Andrea, S.B</creatorcontrib><creatorcontrib>Wakefield, T</creatorcontrib><creatorcontrib>Romagnoli, M</creatorcontrib><creatorcontrib>Chapin, K.C</creatorcontrib><creatorcontrib>Wolk, D.M</creatorcontrib><creatorcontrib>Carroll, K.C</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schieffer, K.M</au><au>Tan, K.E</au><au>Stamper, P.D</au><au>Somogyi, A</au><au>Andrea, S.B</au><au>Wakefield, T</au><au>Romagnoli, M</au><au>Chapin, K.C</au><au>Wolk, D.M</au><au>Carroll, K.C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Multicenter evaluation of the Sepsityper™ extraction kit and MALDI‐TOF MS for direct identification of positive blood culture isolates using the BD BACTEC™ FX and VersaTREK® diagnostic blood culture systems</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2014-04</date><risdate>2014</risdate><volume>116</volume><issue>4</issue><spage>934</spage><epage>941</epage><pages>934-941</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><coden>JAMIFK</coden><abstract>AIMS: (i) Evaluation of delayed time to blood culture extraction by the Sepsityper kit and impact of shipping pellets off‐site for MALDI‐TOF MS analysis. (ii) Comparison of Sepsityper and laboratory‐developed extraction methods from a literature review. METHODS AND RESULTS: Using two blood culture systems (BD BACTEC and VersaTREK), we extracted 411 positive blood cultures using the Sepsityper kit to mimic a potential protocol for institutions without a MALDI‐TOF MS. Extracted pellets were shipped and analysed on the Bruker UltraflexIII. Successful extraction of 358 (87·1%) samples was determined by the presence of detectable proteins. MALDI‐TOF MS correctly identified 332 (80·8%) samples. CONCLUSIONS: Delayed time to extraction did not affect Sepsityper extraction or MALDI‐TOF MS accuracy. The extracted pellets remain stable and provide accurate results by MALDI‐TOF MS when shipped at room temperature to off‐site reference laboratories. SIGNIFICANCE AND IMPACT OF THE STUDY: This is the first study to show that institutions without a MALDI‐TOF MS can take advantage of this innovative technology by shipping a volume of blood to an off‐site laboratory for extraction and MALDI‐TOF MS analysis. We also performed a literature review to compare various extraction methods.</abstract><cop>Oxford</cop><pub>Blackwell Science</pub><pmid>24410849</pmid><doi>10.1111/jam.12434</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Journal of applied microbiology, 2014-04, Vol.116 (4), p.934-941 |
| issn | 1364-5072 1365-2672 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Oxford University Press Journals All Titles (1996-Current) |
| subjects | bacteraemia Bacteremia - diagnosis Bacteria - isolation & purification Bacterial Proteins - analysis Bacterial Proteins - isolation & purification Bacteriological Techniques - methods Biological and medical sciences Biotechnology Blood blood cultures blood volume Fundamental and applied biological sciences. Psychology Humans MALDI‐TOF MS matrix-assisted laser desorption-ionization mass spectrometry Microbiology pellets proteins sepsis Sepsityper shipping Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods technology temperature |
| title | Multicenter evaluation of the Sepsityper™ extraction kit and MALDI‐TOF MS for direct identification of positive blood culture isolates using the BD BACTEC™ FX and VersaTREK® diagnostic blood culture systems |
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