miR-625 down-regulation promotes proliferation and invasion in esophageal cancer by targeting Sox2
•Expression of miR-625 in EC specimens was significantly lower than in corresponding adjacent tissues.•Low expression of miR-625 positively correlated with the proliferation and invasion of EC cell lines in vitro.•Direct binding of miR-625 to the Sox2 3′ UTR was demonstrated.•miR-625 overexpression...
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| Veröffentlicht in: | FEBS letters 2014-03, Vol.588 (6), p.915-921 |
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| creator | Wang, Zhiqiang Qiao, Qiao Chen, Min Li, Xianhua Wang, Zhenjun Liu, Chuanxin Xie, Zongtao |
| description | •Expression of miR-625 in EC specimens was significantly lower than in corresponding adjacent tissues.•Low expression of miR-625 positively correlated with the proliferation and invasion of EC cell lines in vitro.•Direct binding of miR-625 to the Sox2 3′ UTR was demonstrated.•miR-625 overexpression diminished but miR-625 knockdown increased Sox2 expression levels in EC cells.•miR-625 is related to proliferation and invasion of EC by regulating Sox2.
miR-625 has been reported to exhibit abnormal expression in esophageal cancer (EC), but the mechanism and functions of miR-625 in esophageal cancer remain unclear. miR-625 down-regulation and Sox2 up-regulation were validated by qRT-PCR in 158 EC samples. Low expression of miR-625 promotes cell proliferation and invasion, while high expression of miR-625 has the opposite effect. Sox2, a target gene of miR-625, was examined by luciferase assay and western blot. Our data suggest that miR-625 may regulate the biological processes of EC via controlling Sox2 expression. |
| doi_str_mv | 10.1016/j.febslet.2014.01.035 |
| format | Article |
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miR-625 has been reported to exhibit abnormal expression in esophageal cancer (EC), but the mechanism and functions of miR-625 in esophageal cancer remain unclear. miR-625 down-regulation and Sox2 up-regulation were validated by qRT-PCR in 158 EC samples. Low expression of miR-625 promotes cell proliferation and invasion, while high expression of miR-625 has the opposite effect. Sox2, a target gene of miR-625, was examined by luciferase assay and western blot. Our data suggest that miR-625 may regulate the biological processes of EC via controlling Sox2 expression.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/j.febslet.2014.01.035</identifier><identifier>PMID: 24508466</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>3' Untranslated Regions ; Adenocarcinoma - genetics ; Adenocarcinoma - metabolism ; Adenocarcinoma - secondary ; Aged ; Apoptosis ; Base Sequence ; Binding Sites ; Carcinoma, Squamous Cell - genetics ; Carcinoma, Squamous Cell - metabolism ; Carcinoma, Squamous Cell - secondary ; Cell Line, Tumor ; Cell Movement ; Cell Proliferation ; Down-Regulation ; Esophageal cancer ; Esophageal Neoplasms - genetics ; Esophageal Neoplasms - metabolism ; Esophageal Neoplasms - pathology ; Female ; Gene Expression Regulation, Neoplastic ; Humans ; Invasion ; Male ; MicroRNAs - genetics ; MicroRNAs - metabolism ; Middle Aged ; miR-625 ; Neoplasm Invasiveness ; Proliferation ; RNA Interference ; Sox2 ; SOXB1 Transcription Factors - genetics ; SOXB1 Transcription Factors - metabolism</subject><ispartof>FEBS letters, 2014-03, Vol.588 (6), p.915-921</ispartof><rights>2014 Federation of European Biochemical Societies</rights><rights>FEBS Letters 588 (2014) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><rights>Copyright © 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5437-c17169d709925136170e25debf7df54e16cb46348f863f95efddc88218f55863</citedby><cites>FETCH-LOGICAL-c5437-c17169d709925136170e25debf7df54e16cb46348f863f95efddc88218f55863</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2Fj.febslet.2014.01.035$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0014579314000647$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,1411,1427,3537,27901,27902,45550,45551,46384,46808,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24508466$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Zhiqiang</creatorcontrib><creatorcontrib>Qiao, Qiao</creatorcontrib><creatorcontrib>Chen, Min</creatorcontrib><creatorcontrib>Li, Xianhua</creatorcontrib><creatorcontrib>Wang, Zhenjun</creatorcontrib><creatorcontrib>Liu, Chuanxin</creatorcontrib><creatorcontrib>Xie, Zongtao</creatorcontrib><title>miR-625 down-regulation promotes proliferation and invasion in esophageal cancer by targeting Sox2</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>•Expression of miR-625 in EC specimens was significantly lower than in corresponding adjacent tissues.•Low expression of miR-625 positively correlated with the proliferation and invasion of EC cell lines in vitro.•Direct binding of miR-625 to the Sox2 3′ UTR was demonstrated.•miR-625 overexpression diminished but miR-625 knockdown increased Sox2 expression levels in EC cells.•miR-625 is related to proliferation and invasion of EC by regulating Sox2.
miR-625 has been reported to exhibit abnormal expression in esophageal cancer (EC), but the mechanism and functions of miR-625 in esophageal cancer remain unclear. miR-625 down-regulation and Sox2 up-regulation were validated by qRT-PCR in 158 EC samples. Low expression of miR-625 promotes cell proliferation and invasion, while high expression of miR-625 has the opposite effect. Sox2, a target gene of miR-625, was examined by luciferase assay and western blot. Our data suggest that miR-625 may regulate the biological processes of EC via controlling Sox2 expression.</description><subject>3' Untranslated Regions</subject><subject>Adenocarcinoma - genetics</subject><subject>Adenocarcinoma - metabolism</subject><subject>Adenocarcinoma - secondary</subject><subject>Aged</subject><subject>Apoptosis</subject><subject>Base Sequence</subject><subject>Binding Sites</subject><subject>Carcinoma, Squamous Cell - genetics</subject><subject>Carcinoma, Squamous Cell - metabolism</subject><subject>Carcinoma, Squamous Cell - secondary</subject><subject>Cell Line, Tumor</subject><subject>Cell Movement</subject><subject>Cell Proliferation</subject><subject>Down-Regulation</subject><subject>Esophageal cancer</subject><subject>Esophageal Neoplasms - genetics</subject><subject>Esophageal Neoplasms - metabolism</subject><subject>Esophageal Neoplasms - pathology</subject><subject>Female</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Humans</subject><subject>Invasion</subject><subject>Male</subject><subject>MicroRNAs - genetics</subject><subject>MicroRNAs - metabolism</subject><subject>Middle Aged</subject><subject>miR-625</subject><subject>Neoplasm Invasiveness</subject><subject>Proliferation</subject><subject>RNA Interference</subject><subject>Sox2</subject><subject>SOXB1 Transcription Factors - genetics</subject><subject>SOXB1 Transcription Factors - metabolism</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1v1DAQhq0KRLeFnwDKkUuCv-2cEFT9QKqERHu3HHu89Sofi51t2X-Poyxc4WTP-J13xs8g9J7ghmAiP-2aAF3uYW4oJrzBpMFMnKEN0YrVjEv9Cm1weamFatk5ush5h0usSfsGnVMusOZSblA3xB-1pKLy08tYJ9geejvHaaz2aRqmGfJy6WOAtKbt6Ks4Ptu8BHGsIE_7J7sF21fOjg5S1R2r2aYtzHHcVg_TL_oWvQ62z_DudF6ix5vrx6u7-v777berL_e1E5yp2hFFZOsVblsqCJNEYaDCQxeUD4IDka7jknEdtGShFRC8d1pTooMQJXWJPq62ZeCfB8izGWJ20Pd2hOmQDVm-TKnirEjFKnVpyjlBMPsUB5uOhmCz0DU7c6JrFroGE1PolroPpxaHbgD_t-oPziK4WwUvsYfj_7mam-uv9GFZ1bIpwsuWJFfF6vNqBQXZc4RksotQCPuYwM3GT_Ef0_4GDSOjOQ</recordid><startdate>20140318</startdate><enddate>20140318</enddate><creator>Wang, Zhiqiang</creator><creator>Qiao, Qiao</creator><creator>Chen, Min</creator><creator>Li, Xianhua</creator><creator>Wang, Zhenjun</creator><creator>Liu, Chuanxin</creator><creator>Xie, Zongtao</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20140318</creationdate><title>miR-625 down-regulation promotes proliferation and invasion in esophageal cancer by targeting Sox2</title><author>Wang, Zhiqiang ; Qiao, Qiao ; Chen, Min ; Li, Xianhua ; Wang, Zhenjun ; Liu, Chuanxin ; Xie, Zongtao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5437-c17169d709925136170e25debf7df54e16cb46348f863f95efddc88218f55863</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>3' Untranslated Regions</topic><topic>Adenocarcinoma - genetics</topic><topic>Adenocarcinoma - metabolism</topic><topic>Adenocarcinoma - secondary</topic><topic>Aged</topic><topic>Apoptosis</topic><topic>Base Sequence</topic><topic>Binding Sites</topic><topic>Carcinoma, Squamous Cell - genetics</topic><topic>Carcinoma, Squamous Cell - metabolism</topic><topic>Carcinoma, Squamous Cell - secondary</topic><topic>Cell Line, Tumor</topic><topic>Cell Movement</topic><topic>Cell Proliferation</topic><topic>Down-Regulation</topic><topic>Esophageal cancer</topic><topic>Esophageal Neoplasms - genetics</topic><topic>Esophageal Neoplasms - metabolism</topic><topic>Esophageal Neoplasms - pathology</topic><topic>Female</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Humans</topic><topic>Invasion</topic><topic>Male</topic><topic>MicroRNAs - genetics</topic><topic>MicroRNAs - metabolism</topic><topic>Middle Aged</topic><topic>miR-625</topic><topic>Neoplasm Invasiveness</topic><topic>Proliferation</topic><topic>RNA Interference</topic><topic>Sox2</topic><topic>SOXB1 Transcription Factors - genetics</topic><topic>SOXB1 Transcription Factors - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Zhiqiang</creatorcontrib><creatorcontrib>Qiao, Qiao</creatorcontrib><creatorcontrib>Chen, Min</creatorcontrib><creatorcontrib>Li, Xianhua</creatorcontrib><creatorcontrib>Wang, Zhenjun</creatorcontrib><creatorcontrib>Liu, Chuanxin</creatorcontrib><creatorcontrib>Xie, Zongtao</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Zhiqiang</au><au>Qiao, Qiao</au><au>Chen, Min</au><au>Li, Xianhua</au><au>Wang, Zhenjun</au><au>Liu, Chuanxin</au><au>Xie, Zongtao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>miR-625 down-regulation promotes proliferation and invasion in esophageal cancer by targeting Sox2</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>2014-03-18</date><risdate>2014</risdate><volume>588</volume><issue>6</issue><spage>915</spage><epage>921</epage><pages>915-921</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>•Expression of miR-625 in EC specimens was significantly lower than in corresponding adjacent tissues.•Low expression of miR-625 positively correlated with the proliferation and invasion of EC cell lines in vitro.•Direct binding of miR-625 to the Sox2 3′ UTR was demonstrated.•miR-625 overexpression diminished but miR-625 knockdown increased Sox2 expression levels in EC cells.•miR-625 is related to proliferation and invasion of EC by regulating Sox2.
miR-625 has been reported to exhibit abnormal expression in esophageal cancer (EC), but the mechanism and functions of miR-625 in esophageal cancer remain unclear. miR-625 down-regulation and Sox2 up-regulation were validated by qRT-PCR in 158 EC samples. Low expression of miR-625 promotes cell proliferation and invasion, while high expression of miR-625 has the opposite effect. Sox2, a target gene of miR-625, was examined by luciferase assay and western blot. Our data suggest that miR-625 may regulate the biological processes of EC via controlling Sox2 expression.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>24508466</pmid><doi>10.1016/j.febslet.2014.01.035</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Elsevier ScienceDirect Journals; Wiley Online Library Free Content; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | 3' Untranslated Regions Adenocarcinoma - genetics Adenocarcinoma - metabolism Adenocarcinoma - secondary Aged Apoptosis Base Sequence Binding Sites Carcinoma, Squamous Cell - genetics Carcinoma, Squamous Cell - metabolism Carcinoma, Squamous Cell - secondary Cell Line, Tumor Cell Movement Cell Proliferation Down-Regulation Esophageal cancer Esophageal Neoplasms - genetics Esophageal Neoplasms - metabolism Esophageal Neoplasms - pathology Female Gene Expression Regulation, Neoplastic Humans Invasion Male MicroRNAs - genetics MicroRNAs - metabolism Middle Aged miR-625 Neoplasm Invasiveness Proliferation RNA Interference Sox2 SOXB1 Transcription Factors - genetics SOXB1 Transcription Factors - metabolism |
| title | miR-625 down-regulation promotes proliferation and invasion in esophageal cancer by targeting Sox2 |
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