Upconversion fluorescent strip sensor for rapid determination of Vibrio anguillarum
Here, we report a simple and ultrasensitive upconversion fluorescent strip sensor based on NaYF 4 :Yb,Er nanoparticles (NPs) and the lateral flow immunochromatographic assay (LFIA). Carboxyl-modified β-NaYF 4 :Yb,Er NPs were successfully synthesized by a facile one-pot solvothermal approach, upon fu...
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| Veröffentlicht in: | Nanoscale 2014-04, Vol.6 (7), p.384-389 |
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| creator | Zhao, Peng Wu, Yuanyuan Zhu, Yihua Yang, Xiaoling Jiang, Xin Xiao, Jingfan Zhang, Yuanxing Li, Chunzhong |
| description | Here, we report a simple and ultrasensitive upconversion fluorescent strip sensor based on NaYF
4
:Yb,Er nanoparticles (NPs) and the lateral flow immunochromatographic assay (LFIA). Carboxyl-modified β-NaYF
4
:Yb,Er NPs were successfully synthesized by a facile one-pot solvothermal approach, upon further coupling with monoclonal antibody, the resultant UCNPs-antibody conjugates probes were used in LFIA and served as signal vehicles for the fluorescent reporters.
V. anguillarum
was used as a model analyte to demonstrate the use of this strip sensor. The limit of the detection for the fluorescent strip was determined as 10
2
CFU mL
−1
, which is 100 times lower than those displayed by enzyme-linked immunosorbent assays, while the time needed for the detection was only 15 min. Furthermore, no cross-reaction with other eight pathogens was found, indicating the good specificity of the strip. This developed LFIA would offer the potential as a useful tool for the quantification of pathogens analysis in the future.
We report a simple and ultrasensitive UC fluorescent strip sensor based on NaYF
4
:Yb,Er nanoparticles and the LFIA. The limit of the detection for the fluorescent strip was determined as 10
2
CFU mL
−1
; good specificity and stability of the strip sensor were also demonstrated. |
| doi_str_mv | 10.1039/c3nr06549a |
| format | Article |
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4
:Yb,Er nanoparticles (NPs) and the lateral flow immunochromatographic assay (LFIA). Carboxyl-modified β-NaYF
4
:Yb,Er NPs were successfully synthesized by a facile one-pot solvothermal approach, upon further coupling with monoclonal antibody, the resultant UCNPs-antibody conjugates probes were used in LFIA and served as signal vehicles for the fluorescent reporters.
V. anguillarum
was used as a model analyte to demonstrate the use of this strip sensor. The limit of the detection for the fluorescent strip was determined as 10
2
CFU mL
−1
, which is 100 times lower than those displayed by enzyme-linked immunosorbent assays, while the time needed for the detection was only 15 min. Furthermore, no cross-reaction with other eight pathogens was found, indicating the good specificity of the strip. This developed LFIA would offer the potential as a useful tool for the quantification of pathogens analysis in the future.
We report a simple and ultrasensitive UC fluorescent strip sensor based on NaYF
4
:Yb,Er nanoparticles and the LFIA. The limit of the detection for the fluorescent strip was determined as 10
2
CFU mL
−1
; good specificity and stability of the strip sensor were also demonstrated.</description><identifier>ISSN: 2040-3364</identifier><identifier>EISSN: 2040-3372</identifier><identifier>DOI: 10.1039/c3nr06549a</identifier><identifier>PMID: 24577708</identifier><language>eng</language><publisher>England</publisher><subject>Antibodies, Monoclonal - chemistry ; Antibodies, Monoclonal - immunology ; Antigens - analysis ; Antigens - immunology ; Cross Reactions ; Erbium - chemistry ; Fluorescent Dyes - chemistry ; Fluorides - chemistry ; Immunochromatography ; Nanoparticles - chemistry ; Vibrio - isolation & purification ; Vibrio - metabolism ; Vibrio anguillarum ; Ytterbium - chemistry ; Yttrium - chemistry</subject><ispartof>Nanoscale, 2014-04, Vol.6 (7), p.384-389</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c475t-768eb7b2dd5e08957ec6c6ed285715de84bf283fc35596e49a2cdb5f3f3fdaef3</citedby><cites>FETCH-LOGICAL-c475t-768eb7b2dd5e08957ec6c6ed285715de84bf283fc35596e49a2cdb5f3f3fdaef3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24577708$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhao, Peng</creatorcontrib><creatorcontrib>Wu, Yuanyuan</creatorcontrib><creatorcontrib>Zhu, Yihua</creatorcontrib><creatorcontrib>Yang, Xiaoling</creatorcontrib><creatorcontrib>Jiang, Xin</creatorcontrib><creatorcontrib>Xiao, Jingfan</creatorcontrib><creatorcontrib>Zhang, Yuanxing</creatorcontrib><creatorcontrib>Li, Chunzhong</creatorcontrib><title>Upconversion fluorescent strip sensor for rapid determination of Vibrio anguillarum</title><title>Nanoscale</title><addtitle>Nanoscale</addtitle><description>Here, we report a simple and ultrasensitive upconversion fluorescent strip sensor based on NaYF
4
:Yb,Er nanoparticles (NPs) and the lateral flow immunochromatographic assay (LFIA). Carboxyl-modified β-NaYF
4
:Yb,Er NPs were successfully synthesized by a facile one-pot solvothermal approach, upon further coupling with monoclonal antibody, the resultant UCNPs-antibody conjugates probes were used in LFIA and served as signal vehicles for the fluorescent reporters.
V. anguillarum
was used as a model analyte to demonstrate the use of this strip sensor. The limit of the detection for the fluorescent strip was determined as 10
2
CFU mL
−1
, which is 100 times lower than those displayed by enzyme-linked immunosorbent assays, while the time needed for the detection was only 15 min. Furthermore, no cross-reaction with other eight pathogens was found, indicating the good specificity of the strip. This developed LFIA would offer the potential as a useful tool for the quantification of pathogens analysis in the future.
We report a simple and ultrasensitive UC fluorescent strip sensor based on NaYF
4
:Yb,Er nanoparticles and the LFIA. The limit of the detection for the fluorescent strip was determined as 10
2
CFU mL
−1
; good specificity and stability of the strip sensor were also demonstrated.</description><subject>Antibodies, Monoclonal - chemistry</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Antigens - analysis</subject><subject>Antigens - immunology</subject><subject>Cross Reactions</subject><subject>Erbium - chemistry</subject><subject>Fluorescent Dyes - chemistry</subject><subject>Fluorides - chemistry</subject><subject>Immunochromatography</subject><subject>Nanoparticles - chemistry</subject><subject>Vibrio - isolation & purification</subject><subject>Vibrio - metabolism</subject><subject>Vibrio anguillarum</subject><subject>Ytterbium - chemistry</subject><subject>Yttrium - chemistry</subject><issn>2040-3364</issn><issn>2040-3372</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1LxDAQxYMorq5evCv1JkI1bZqkPS6LX7AoqOu1pMlEItukJq3gf2_WXdebyBBm4P3ymLwgdJThiwyT6lIS6zGjRSW20F6OC5wSwvPtzcyKEdoP4Q1jVhFGdtEoLyjnHJd76GneSWc_wAfjbKIXg_MQJNg-Cb03XRLABucTHY8XnVGJgh58a6zolxecTl5M441LhH0dzGIh_NAeoB0tFgEO132M5tdXz9PbdPZwczedzFJZcNqnnJXQ8CZXigIuK8pBMslA5SXlGVVQFo3OS6IlobRiEJ-XS9VQTWIpAZqM0dnKt_PufYDQ162Ju8clLLgh1BnN8phEgat_oJjzqqKsjOj5CpXeheBB1503rfCfdYbrZd71lNw_fuc9ifDJ2ndoWlAb9CfgCByvAB_kRv39sKif_qXXndLkC5BYkjE</recordid><startdate>20140407</startdate><enddate>20140407</enddate><creator>Zhao, Peng</creator><creator>Wu, Yuanyuan</creator><creator>Zhu, Yihua</creator><creator>Yang, Xiaoling</creator><creator>Jiang, Xin</creator><creator>Xiao, Jingfan</creator><creator>Zhang, Yuanxing</creator><creator>Li, Chunzhong</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>20140407</creationdate><title>Upconversion fluorescent strip sensor for rapid determination of Vibrio anguillarum</title><author>Zhao, Peng ; Wu, Yuanyuan ; Zhu, Yihua ; Yang, Xiaoling ; Jiang, Xin ; Xiao, Jingfan ; Zhang, Yuanxing ; Li, Chunzhong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c475t-768eb7b2dd5e08957ec6c6ed285715de84bf283fc35596e49a2cdb5f3f3fdaef3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Antibodies, Monoclonal - chemistry</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Antigens - analysis</topic><topic>Antigens - immunology</topic><topic>Cross Reactions</topic><topic>Erbium - chemistry</topic><topic>Fluorescent Dyes - chemistry</topic><topic>Fluorides - chemistry</topic><topic>Immunochromatography</topic><topic>Nanoparticles - chemistry</topic><topic>Vibrio - isolation & purification</topic><topic>Vibrio - metabolism</topic><topic>Vibrio anguillarum</topic><topic>Ytterbium - chemistry</topic><topic>Yttrium - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhao, Peng</creatorcontrib><creatorcontrib>Wu, Yuanyuan</creatorcontrib><creatorcontrib>Zhu, Yihua</creatorcontrib><creatorcontrib>Yang, Xiaoling</creatorcontrib><creatorcontrib>Jiang, Xin</creatorcontrib><creatorcontrib>Xiao, Jingfan</creatorcontrib><creatorcontrib>Zhang, Yuanxing</creatorcontrib><creatorcontrib>Li, Chunzhong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Nanoscale</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhao, Peng</au><au>Wu, Yuanyuan</au><au>Zhu, Yihua</au><au>Yang, Xiaoling</au><au>Jiang, Xin</au><au>Xiao, Jingfan</au><au>Zhang, Yuanxing</au><au>Li, Chunzhong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Upconversion fluorescent strip sensor for rapid determination of Vibrio anguillarum</atitle><jtitle>Nanoscale</jtitle><addtitle>Nanoscale</addtitle><date>2014-04-07</date><risdate>2014</risdate><volume>6</volume><issue>7</issue><spage>384</spage><epage>389</epage><pages>384-389</pages><issn>2040-3364</issn><eissn>2040-3372</eissn><abstract>Here, we report a simple and ultrasensitive upconversion fluorescent strip sensor based on NaYF
4
:Yb,Er nanoparticles (NPs) and the lateral flow immunochromatographic assay (LFIA). Carboxyl-modified β-NaYF
4
:Yb,Er NPs were successfully synthesized by a facile one-pot solvothermal approach, upon further coupling with monoclonal antibody, the resultant UCNPs-antibody conjugates probes were used in LFIA and served as signal vehicles for the fluorescent reporters.
V. anguillarum
was used as a model analyte to demonstrate the use of this strip sensor. The limit of the detection for the fluorescent strip was determined as 10
2
CFU mL
−1
, which is 100 times lower than those displayed by enzyme-linked immunosorbent assays, while the time needed for the detection was only 15 min. Furthermore, no cross-reaction with other eight pathogens was found, indicating the good specificity of the strip. This developed LFIA would offer the potential as a useful tool for the quantification of pathogens analysis in the future.
We report a simple and ultrasensitive UC fluorescent strip sensor based on NaYF
4
:Yb,Er nanoparticles and the LFIA. The limit of the detection for the fluorescent strip was determined as 10
2
CFU mL
−1
; good specificity and stability of the strip sensor were also demonstrated.</abstract><cop>England</cop><pmid>24577708</pmid><doi>10.1039/c3nr06549a</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2040-3364 |
| ispartof | Nanoscale, 2014-04, Vol.6 (7), p.384-389 |
| issn | 2040-3364 2040-3372 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1507799568 |
| source | MEDLINE; Royal Society Of Chemistry Journals; Alma/SFX Local Collection |
| subjects | Antibodies, Monoclonal - chemistry Antibodies, Monoclonal - immunology Antigens - analysis Antigens - immunology Cross Reactions Erbium - chemistry Fluorescent Dyes - chemistry Fluorides - chemistry Immunochromatography Nanoparticles - chemistry Vibrio - isolation & purification Vibrio - metabolism Vibrio anguillarum Ytterbium - chemistry Yttrium - chemistry |
| title | Upconversion fluorescent strip sensor for rapid determination of Vibrio anguillarum |
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