Antiviral Activity of Polymethoxylated Flavones from “Guangchenpi”, the Edible and Medicinal Pericarps of Citrus reticulata ‘Chachi’
The present study found that the supercritical fluid extract of “Guangchenpi” possessed in vitro antiviral activity against respiratory syncytial virus (RSV). Bioassay-guided isolation and identification of this extract led to obtain five active polymethoxylated flavones (1–5). Cytopathic effect (CP...
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| Veröffentlicht in: | Journal of agricultural and food chemistry 2014-03, Vol.62 (10), p.2182-2189 |
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| creator | Xu, Jiao-Jiao Wu, Xia Li, Man-Mei Li, Guo-Qiang Yang, Yi-Ting Luo, Hu-Jie Huang, Wei-Huang Chung, Hau Yin Ye, Wen-Cai Wang, Guo-Cai Li, Yao-Lan |
| description | The present study found that the supercritical fluid extract of “Guangchenpi” possessed in vitro antiviral activity against respiratory syncytial virus (RSV). Bioassay-guided isolation and identification of this extract led to obtain five active polymethoxylated flavones (1–5). Cytopathic effect (CPE) reduction assay exhibited that tangeretin (2) and nobiletin (3), two major polymethoxylated flavones in the extract, possessed better anti-RSV effect comparable to the positive control ribavirin. Plaque reduction assay revealed that tangeretin dose-dependently inhibited RSV-induced plaque formation on the HEp-2 cells. This polymethoxylated flavone mainly affected the intracellular replication of RSV, and it also could inhibit RSV entry into the HEp-2 cells. Further investigations with quantitative real-time PCR and confocal and Western blot assays indicated that tangeretin downregulated the expression of RSV phosphoprotein (P protein). Results suggest the potential application of the supercritical fluid extract of “Guangchenpi” and tangeretin in the treatment and the prevention of RSV infection. |
| doi_str_mv | 10.1021/jf404310y |
| format | Article |
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Bioassay-guided isolation and identification of this extract led to obtain five active polymethoxylated flavones (1–5). Cytopathic effect (CPE) reduction assay exhibited that tangeretin (2) and nobiletin (3), two major polymethoxylated flavones in the extract, possessed better anti-RSV effect comparable to the positive control ribavirin. Plaque reduction assay revealed that tangeretin dose-dependently inhibited RSV-induced plaque formation on the HEp-2 cells. This polymethoxylated flavone mainly affected the intracellular replication of RSV, and it also could inhibit RSV entry into the HEp-2 cells. Further investigations with quantitative real-time PCR and confocal and Western blot assays indicated that tangeretin downregulated the expression of RSV phosphoprotein (P protein). Results suggest the potential application of the supercritical fluid extract of “Guangchenpi” and tangeretin in the treatment and the prevention of RSV infection.</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/jf404310y</identifier><identifier>PMID: 24377463</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Antiviral Agents - chemistry ; Antiviral Agents - pharmacology ; antiviral properties ; Cell Line - drug effects ; Cell Line - virology ; Citrus - chemistry ; Citrus reticulata ; cytopathogenicity ; Dose-Response Relationship, Drug ; Drug Evaluation, Preclinical - methods ; flavones ; Flavones - chemistry ; Flavones - isolation & purification ; Flavones - pharmacology ; gene expression regulation ; human cell lines ; Humans ; phosphoproteins ; Plant Extracts - chemistry ; Plants, Medicinal - chemistry ; quantitative polymerase chain reaction ; Respiratory Syncytial Viruses - drug effects ; Respiratory Syncytial Viruses - genetics ; Respiratory Syncytial Viruses - pathogenicity ; Viral Structural Proteins - metabolism ; Virus Attachment - drug effects ; viruses ; Western blotting</subject><ispartof>Journal of agricultural and food chemistry, 2014-03, Vol.62 (10), p.2182-2189</ispartof><rights>Copyright © 2013 American Chemical Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a320t-42678baf4d98cd8949911bfd462b96eca7364488c3330a8bbf52f3b25ed8c6993</citedby><cites>FETCH-LOGICAL-a320t-42678baf4d98cd8949911bfd462b96eca7364488c3330a8bbf52f3b25ed8c6993</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/jf404310y$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/jf404310y$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24377463$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xu, Jiao-Jiao</creatorcontrib><creatorcontrib>Wu, Xia</creatorcontrib><creatorcontrib>Li, Man-Mei</creatorcontrib><creatorcontrib>Li, Guo-Qiang</creatorcontrib><creatorcontrib>Yang, Yi-Ting</creatorcontrib><creatorcontrib>Luo, Hu-Jie</creatorcontrib><creatorcontrib>Huang, Wei-Huang</creatorcontrib><creatorcontrib>Chung, Hau Yin</creatorcontrib><creatorcontrib>Ye, Wen-Cai</creatorcontrib><creatorcontrib>Wang, Guo-Cai</creatorcontrib><creatorcontrib>Li, Yao-Lan</creatorcontrib><title>Antiviral Activity of Polymethoxylated Flavones from “Guangchenpi”, the Edible and Medicinal Pericarps of Citrus reticulata ‘Chachi’</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>The present study found that the supercritical fluid extract of “Guangchenpi” possessed in vitro antiviral activity against respiratory syncytial virus (RSV). Bioassay-guided isolation and identification of this extract led to obtain five active polymethoxylated flavones (1–5). Cytopathic effect (CPE) reduction assay exhibited that tangeretin (2) and nobiletin (3), two major polymethoxylated flavones in the extract, possessed better anti-RSV effect comparable to the positive control ribavirin. Plaque reduction assay revealed that tangeretin dose-dependently inhibited RSV-induced plaque formation on the HEp-2 cells. This polymethoxylated flavone mainly affected the intracellular replication of RSV, and it also could inhibit RSV entry into the HEp-2 cells. Further investigations with quantitative real-time PCR and confocal and Western blot assays indicated that tangeretin downregulated the expression of RSV phosphoprotein (P protein). Results suggest the potential application of the supercritical fluid extract of “Guangchenpi” and tangeretin in the treatment and the prevention of RSV infection.</description><subject>Antiviral Agents - chemistry</subject><subject>Antiviral Agents - pharmacology</subject><subject>antiviral properties</subject><subject>Cell Line - drug effects</subject><subject>Cell Line - virology</subject><subject>Citrus - chemistry</subject><subject>Citrus reticulata</subject><subject>cytopathogenicity</subject><subject>Dose-Response Relationship, Drug</subject><subject>Drug Evaluation, Preclinical - methods</subject><subject>flavones</subject><subject>Flavones - chemistry</subject><subject>Flavones - isolation & purification</subject><subject>Flavones - pharmacology</subject><subject>gene expression regulation</subject><subject>human cell lines</subject><subject>Humans</subject><subject>phosphoproteins</subject><subject>Plant Extracts - chemistry</subject><subject>Plants, Medicinal - chemistry</subject><subject>quantitative polymerase chain reaction</subject><subject>Respiratory Syncytial Viruses - drug effects</subject><subject>Respiratory Syncytial Viruses - genetics</subject><subject>Respiratory Syncytial Viruses - pathogenicity</subject><subject>Viral Structural Proteins - metabolism</subject><subject>Virus Attachment - drug effects</subject><subject>viruses</subject><subject>Western blotting</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkE1u1DAUgC0EokNhwQXAGySQCPgvibMcjdqCVEQl6Np6cezGoyQebKciux6AA4AEl-tJ8GhKV6zek96n70kfQs8peUcJo--3VhDBKVkeoBUtGSlKSuVDtCL5WMiyokfoSYxbQogsa_IYHTHB61pUfIV-rKfkrl2AAa_1fksL9hZf-GEZTer992WAZDp8OsC1n0zENvgR3978PpthutK9mXbu9ubPW5x6g0861w4Gw9ThT6Zz2k1Ze2GC0xB2ce_duBTmiINJTs_ZDFn1c9OD7rPl11P0yMIQzbO7eYwuT0--bj4U55_PPm7W5wVwRlIhWFXLFqzoGqk72YimobS1nahY21RGQ80rIaTUnHMCsm1tySxvWWk6qaum4cfo9cG7C_7bbGJSo4vaDANMxs9R0ZLUVMq6YRl9c0B18DEGY9UuuBHCoihR-_jqPn5mX9xp53Y03T35r3YGXh4AC17BVXBRXX5hhFaEUCqYFJl4dSBAR7X1c8gF439e_QVLcZmp</recordid><startdate>20140312</startdate><enddate>20140312</enddate><creator>Xu, Jiao-Jiao</creator><creator>Wu, Xia</creator><creator>Li, Man-Mei</creator><creator>Li, Guo-Qiang</creator><creator>Yang, Yi-Ting</creator><creator>Luo, Hu-Jie</creator><creator>Huang, Wei-Huang</creator><creator>Chung, Hau Yin</creator><creator>Ye, Wen-Cai</creator><creator>Wang, Guo-Cai</creator><creator>Li, Yao-Lan</creator><general>American Chemical Society</general><general>American Chemical Society, Books and Journals Division</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20140312</creationdate><title>Antiviral Activity of Polymethoxylated Flavones from “Guangchenpi”, the Edible and Medicinal Pericarps of Citrus reticulata ‘Chachi’</title><author>Xu, Jiao-Jiao ; Wu, Xia ; Li, Man-Mei ; Li, Guo-Qiang ; Yang, Yi-Ting ; Luo, Hu-Jie ; Huang, Wei-Huang ; Chung, Hau Yin ; Ye, Wen-Cai ; Wang, Guo-Cai ; Li, Yao-Lan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a320t-42678baf4d98cd8949911bfd462b96eca7364488c3330a8bbf52f3b25ed8c6993</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Antiviral Agents - chemistry</topic><topic>Antiviral Agents - pharmacology</topic><topic>antiviral properties</topic><topic>Cell Line - drug effects</topic><topic>Cell Line - virology</topic><topic>Citrus - chemistry</topic><topic>Citrus reticulata</topic><topic>cytopathogenicity</topic><topic>Dose-Response Relationship, Drug</topic><topic>Drug Evaluation, Preclinical - methods</topic><topic>flavones</topic><topic>Flavones - chemistry</topic><topic>Flavones - isolation & purification</topic><topic>Flavones - pharmacology</topic><topic>gene expression regulation</topic><topic>human cell lines</topic><topic>Humans</topic><topic>phosphoproteins</topic><topic>Plant Extracts - chemistry</topic><topic>Plants, Medicinal - chemistry</topic><topic>quantitative polymerase chain reaction</topic><topic>Respiratory Syncytial Viruses - drug effects</topic><topic>Respiratory Syncytial Viruses - genetics</topic><topic>Respiratory Syncytial Viruses - pathogenicity</topic><topic>Viral Structural Proteins - metabolism</topic><topic>Virus Attachment - drug effects</topic><topic>viruses</topic><topic>Western blotting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xu, Jiao-Jiao</creatorcontrib><creatorcontrib>Wu, Xia</creatorcontrib><creatorcontrib>Li, Man-Mei</creatorcontrib><creatorcontrib>Li, Guo-Qiang</creatorcontrib><creatorcontrib>Yang, Yi-Ting</creatorcontrib><creatorcontrib>Luo, Hu-Jie</creatorcontrib><creatorcontrib>Huang, Wei-Huang</creatorcontrib><creatorcontrib>Chung, Hau Yin</creatorcontrib><creatorcontrib>Ye, Wen-Cai</creatorcontrib><creatorcontrib>Wang, Guo-Cai</creatorcontrib><creatorcontrib>Li, Yao-Lan</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xu, Jiao-Jiao</au><au>Wu, Xia</au><au>Li, Man-Mei</au><au>Li, Guo-Qiang</au><au>Yang, Yi-Ting</au><au>Luo, Hu-Jie</au><au>Huang, Wei-Huang</au><au>Chung, Hau Yin</au><au>Ye, Wen-Cai</au><au>Wang, Guo-Cai</au><au>Li, Yao-Lan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antiviral Activity of Polymethoxylated Flavones from “Guangchenpi”, the Edible and Medicinal Pericarps of Citrus reticulata ‘Chachi’</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2014-03-12</date><risdate>2014</risdate><volume>62</volume><issue>10</issue><spage>2182</spage><epage>2189</epage><pages>2182-2189</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><abstract>The present study found that the supercritical fluid extract of “Guangchenpi” possessed in vitro antiviral activity against respiratory syncytial virus (RSV). Bioassay-guided isolation and identification of this extract led to obtain five active polymethoxylated flavones (1–5). Cytopathic effect (CPE) reduction assay exhibited that tangeretin (2) and nobiletin (3), two major polymethoxylated flavones in the extract, possessed better anti-RSV effect comparable to the positive control ribavirin. Plaque reduction assay revealed that tangeretin dose-dependently inhibited RSV-induced plaque formation on the HEp-2 cells. This polymethoxylated flavone mainly affected the intracellular replication of RSV, and it also could inhibit RSV entry into the HEp-2 cells. Further investigations with quantitative real-time PCR and confocal and Western blot assays indicated that tangeretin downregulated the expression of RSV phosphoprotein (P protein). Results suggest the potential application of the supercritical fluid extract of “Guangchenpi” and tangeretin in the treatment and the prevention of RSV infection.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>24377463</pmid><doi>10.1021/jf404310y</doi><tpages>8</tpages></addata></record> |
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| subjects | Antiviral Agents - chemistry Antiviral Agents - pharmacology antiviral properties Cell Line - drug effects Cell Line - virology Citrus - chemistry Citrus reticulata cytopathogenicity Dose-Response Relationship, Drug Drug Evaluation, Preclinical - methods flavones Flavones - chemistry Flavones - isolation & purification Flavones - pharmacology gene expression regulation human cell lines Humans phosphoproteins Plant Extracts - chemistry Plants, Medicinal - chemistry quantitative polymerase chain reaction Respiratory Syncytial Viruses - drug effects Respiratory Syncytial Viruses - genetics Respiratory Syncytial Viruses - pathogenicity Viral Structural Proteins - metabolism Virus Attachment - drug effects viruses Western blotting |
| title | Antiviral Activity of Polymethoxylated Flavones from “Guangchenpi”, the Edible and Medicinal Pericarps of Citrus reticulata ‘Chachi’ |
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