Determination of the N-acetyl metabolites of 4,4′-methylene dianiline and 4,4′-methylene-bis(2-chloroaniline) in urine
A sensitive and specific gas chromatographic/mass spectrometric assay is described for the determination of N‐acetyl 4,4′‐methylene dianiline (N‐acetyl MDA) and N‐acetyl 4′4′‐methylene‐bis(2‐chloroaniline) (N‐acetyl MbOCA) in urine. The method is based on the solvent extraction of the compounds toge...
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| Veröffentlicht in: | Biological Mass Spectrometry 1988-09, Vol.17 (3), p.161-167 |
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| container_title | Biological Mass Spectrometry |
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| creator | Cocker, John Boobis, Alan R. Davies, Donald S. |
| description | A sensitive and specific gas chromatographic/mass spectrometric assay is described for the determination of N‐acetyl 4,4′‐methylene dianiline (N‐acetyl MDA) and N‐acetyl 4′4′‐methylene‐bis(2‐chloroaniline) (N‐acetyl MbOCA) in urine. The method is based on the solvent extraction of the compounds together with deuterium‐labelled internal standards, the compounds being separated and detected by capillary gas chromatography/mass spectrometry as their pentafluoropropyl derivatives. The method has been applied to the detection of N‐acetyl MbOCA and N‐acetyl MDA in the urine of workers occupationally exposed to MbOCA and MDA. The results show that whilst N‐acetyl MbOCA is a relatively minor urinary metabolite a significant proportion of MDA is excreted as the N‐acetylated compound. |
| doi_str_mv | 10.1002/bms.1200170303 |
| format | Article |
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The method is based on the solvent extraction of the compounds together with deuterium‐labelled internal standards, the compounds being separated and detected by capillary gas chromatography/mass spectrometry as their pentafluoropropyl derivatives. The method has been applied to the detection of N‐acetyl MbOCA and N‐acetyl MDA in the urine of workers occupationally exposed to MbOCA and MDA. The results show that whilst N‐acetyl MbOCA is a relatively minor urinary metabolite a significant proportion of MDA is excreted as the N‐acetylated compound.</description><identifier>ISSN: 1052-9306</identifier><identifier>ISSN: 0887-6134</identifier><identifier>EISSN: 1096-9888</identifier><identifier>EISSN: 2376-3868</identifier><identifier>DOI: 10.1002/bms.1200170303</identifier><identifier>PMID: 3214672</identifier><identifier>CODEN: BEMSEN</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Acetanilides - urine ; Benzhydryl Compounds - urine ; Biological and medical sciences ; Chemical and industrial products toxicology. Toxic occupational diseases ; Environmental Exposure ; Gas Chromatography-Mass Spectrometry - methods ; Gas, fumes ; Humans ; Medical sciences ; Methylenebis(chloroaniline) - analogs & derivatives ; Methylenebis(chloroaniline) - urine ; Toxicology</subject><ispartof>Biological Mass Spectrometry, 1988-09, Vol.17 (3), p.161-167</ispartof><rights>Copyright © 1988 John Wiley & Sons Ltd.</rights><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fbms.1200170303$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fbms.1200170303$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6774897$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3214672$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cocker, John</creatorcontrib><creatorcontrib>Boobis, Alan R.</creatorcontrib><creatorcontrib>Davies, Donald S.</creatorcontrib><title>Determination of the N-acetyl metabolites of 4,4′-methylene dianiline and 4,4′-methylene-bis(2-chloroaniline) in urine</title><title>Biological Mass Spectrometry</title><addtitle>Biol. Mass Spectrom</addtitle><description>A sensitive and specific gas chromatographic/mass spectrometric assay is described for the determination of N‐acetyl 4,4′‐methylene dianiline (N‐acetyl MDA) and N‐acetyl 4′4′‐methylene‐bis(2‐chloroaniline) (N‐acetyl MbOCA) in urine. The method is based on the solvent extraction of the compounds together with deuterium‐labelled internal standards, the compounds being separated and detected by capillary gas chromatography/mass spectrometry as their pentafluoropropyl derivatives. The method has been applied to the detection of N‐acetyl MbOCA and N‐acetyl MDA in the urine of workers occupationally exposed to MbOCA and MDA. The results show that whilst N‐acetyl MbOCA is a relatively minor urinary metabolite a significant proportion of MDA is excreted as the N‐acetylated compound.</description><subject>Acetanilides - urine</subject><subject>Benzhydryl Compounds - urine</subject><subject>Biological and medical sciences</subject><subject>Chemical and industrial products toxicology. Toxic occupational diseases</subject><subject>Environmental Exposure</subject><subject>Gas Chromatography-Mass Spectrometry - methods</subject><subject>Gas, fumes</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Methylenebis(chloroaniline) - analogs & derivatives</subject><subject>Methylenebis(chloroaniline) - urine</subject><subject>Toxicology</subject><issn>1052-9306</issn><issn>0887-6134</issn><issn>1096-9888</issn><issn>2376-3868</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNplkUFv1DAQhS0EKqVw5YaUA0JUwmUcO7FzhAUWpLYgAerRmjgTrcFJSpxVuz31N_GT-CX1aqPtgZOf_b430vgx9lzAiQDI39ZdPBE5gNAgQT5ghwKqklfGmIdbXeS8klA-Zk9i_AUgTQodsAOZC1Xq_JDdfKCJxs73OPmhz4Y2m1aUnXN0NG1C1tGE9RD8RHHrqTfq3-1fnl5Xm0A9ZY3H3gefFPbNfzavfXydc7cKwzjM4HHm-2w9JvWUPWoxRHo2n0fs56ePPxaf-enX5ZfFu1PuZaEkb0GQrGsoW2oMAgpUaV8thMwb6RAFtA5EIYVWTguJbVWQMU1tRNs4Kmt5xF7t5l6Ow581xcl2PjoKAXsa1tGKAkpVKZnAFzO4rjtq7OXoOxw3dv6s5L-cfYwOQzti73zcY6XWylQ6YdUOu_KBNntbgN0WZlNh9r4w-_7s-_0tZfku6-NE1_ssjr_TeKkLe3G-tMvFhT6rvpVWyztZEJow</recordid><startdate>198809</startdate><enddate>198809</enddate><creator>Cocker, John</creator><creator>Boobis, Alan R.</creator><creator>Davies, Donald S.</creator><general>John Wiley & Sons, Ltd</general><general>Wiley</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>198809</creationdate><title>Determination of the N-acetyl metabolites of 4,4′-methylene dianiline and 4,4′-methylene-bis(2-chloroaniline) in urine</title><author>Cocker, John ; Boobis, Alan R. ; Davies, Donald S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i3543-f01e3bb06fed8a0a1a412071132d3caa10fc0153174c713af95e88db81fdce6b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Acetanilides - urine</topic><topic>Benzhydryl Compounds - urine</topic><topic>Biological and medical sciences</topic><topic>Chemical and industrial products toxicology. Toxic occupational diseases</topic><topic>Environmental Exposure</topic><topic>Gas Chromatography-Mass Spectrometry - methods</topic><topic>Gas, fumes</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Methylenebis(chloroaniline) - analogs & derivatives</topic><topic>Methylenebis(chloroaniline) - urine</topic><topic>Toxicology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cocker, John</creatorcontrib><creatorcontrib>Boobis, Alan R.</creatorcontrib><creatorcontrib>Davies, Donald S.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Biological Mass Spectrometry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cocker, John</au><au>Boobis, Alan R.</au><au>Davies, Donald S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of the N-acetyl metabolites of 4,4′-methylene dianiline and 4,4′-methylene-bis(2-chloroaniline) in urine</atitle><jtitle>Biological Mass Spectrometry</jtitle><addtitle>Biol. Mass Spectrom</addtitle><date>1988-09</date><risdate>1988</risdate><volume>17</volume><issue>3</issue><spage>161</spage><epage>167</epage><pages>161-167</pages><issn>1052-9306</issn><issn>0887-6134</issn><eissn>1096-9888</eissn><eissn>2376-3868</eissn><coden>BEMSEN</coden><abstract>A sensitive and specific gas chromatographic/mass spectrometric assay is described for the determination of N‐acetyl 4,4′‐methylene dianiline (N‐acetyl MDA) and N‐acetyl 4′4′‐methylene‐bis(2‐chloroaniline) (N‐acetyl MbOCA) in urine. The method is based on the solvent extraction of the compounds together with deuterium‐labelled internal standards, the compounds being separated and detected by capillary gas chromatography/mass spectrometry as their pentafluoropropyl derivatives. The method has been applied to the detection of N‐acetyl MbOCA and N‐acetyl MDA in the urine of workers occupationally exposed to MbOCA and MDA. The results show that whilst N‐acetyl MbOCA is a relatively minor urinary metabolite a significant proportion of MDA is excreted as the N‐acetylated compound.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>3214672</pmid><doi>10.1002/bms.1200170303</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1052-9306 |
| ispartof | Biological Mass Spectrometry, 1988-09, Vol.17 (3), p.161-167 |
| issn | 1052-9306 0887-6134 1096-9888 2376-3868 |
| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Alma/SFX Local Collection |
| subjects | Acetanilides - urine Benzhydryl Compounds - urine Biological and medical sciences Chemical and industrial products toxicology. Toxic occupational diseases Environmental Exposure Gas Chromatography-Mass Spectrometry - methods Gas, fumes Humans Medical sciences Methylenebis(chloroaniline) - analogs & derivatives Methylenebis(chloroaniline) - urine Toxicology |
| title | Determination of the N-acetyl metabolites of 4,4′-methylene dianiline and 4,4′-methylene-bis(2-chloroaniline) in urine |
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