Monosaccharide composition of glycans based on Q-HSQC NMR
•A method to determine the monosaccharide composition based on NMR was developed.•Q-HSQC depends of a JCH value of 155Hz for C-1, allowing milli-microM detection.•Analysis of the native and hydrolyzed glycan give insights of the molecular mobility. Glycans have essential functions related to structu...
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| Veröffentlicht in: | Carbohydrate polymers 2014-04, Vol.104, p.34-41 |
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| creator | Sassaki, Guilherme Lanzi Guerrini, Marco Serrato, Rodrigo Vassoler Santana Filho, Arquimedes Paixão Carlotto, Juliane Simas-Tosin, Fernanda Cipriani, Thales Ricardo Iacomini, Marcello Torri, Giangiacomo Gorin, Philip Albert James |
| description | •A method to determine the monosaccharide composition based on NMR was developed.•Q-HSQC depends of a JCH value of 155Hz for C-1, allowing milli-microM detection.•Analysis of the native and hydrolyzed glycan give insights of the molecular mobility.
Glycans have essential functions related to structural architecture and specific cell surface phenomena, such as differentiation, biosignalling, recognition and cell–cell interaction, with the carbohydrate structure determining main function in the cell. Due to the importance of the primary structure, the monosaccharide composition is crucial to show the glycan structure. We now present a method for complex carbohydrates based on NMR spectroscopy, which has shown to give similar results to those obtained by the classic GC–MS-carboxy-reduction/deuterium labeling approach. Quantitative HSQC, through JCH dependence showed 155Hz as the best value for 1H/13C anomeric aldoses, allowing milli-microM detection using conventional inverse probe heads. Combining the quantification of native monosaccharide units of the glycan and those from the hydrolyzed product, a strong correlation occurs between the molecular mobility of the monosaccharide units, giving rise to some insights on the dynamic properties of the parent glycan. |
| doi_str_mv | 10.1016/j.carbpol.2013.12.046 |
| format | Article |
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Glycans have essential functions related to structural architecture and specific cell surface phenomena, such as differentiation, biosignalling, recognition and cell–cell interaction, with the carbohydrate structure determining main function in the cell. Due to the importance of the primary structure, the monosaccharide composition is crucial to show the glycan structure. We now present a method for complex carbohydrates based on NMR spectroscopy, which has shown to give similar results to those obtained by the classic GC–MS-carboxy-reduction/deuterium labeling approach. Quantitative HSQC, through JCH dependence showed 155Hz as the best value for 1H/13C anomeric aldoses, allowing milli-microM detection using conventional inverse probe heads. Combining the quantification of native monosaccharide units of the glycan and those from the hydrolyzed product, a strong correlation occurs between the molecular mobility of the monosaccharide units, giving rise to some insights on the dynamic properties of the parent glycan.</description><identifier>ISSN: 0144-8617</identifier><identifier>EISSN: 1879-1344</identifier><identifier>DOI: 10.1016/j.carbpol.2013.12.046</identifier><identifier>PMID: 24607157</identifier><identifier>CODEN: CAPOD8</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>Applied sciences ; Carbohydrate quantification ; Dynamics ; Exact sciences and technology ; Glycoconjugates ; Magnetic Resonance Spectroscopy - methods ; Monosaccharides - analysis ; Mutarotation ; Natural polymers ; Physicochemistry of polymers ; Polysaccharides ; Polysaccharides - chemistry ; Starch and polysaccharides</subject><ispartof>Carbohydrate polymers, 2014-04, Vol.104, p.34-41</ispartof><rights>2013 Elsevier Ltd</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2013 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c395t-1abd1e2f9e10d0c24d447c1087150b9f08f5729c6fe0c2bf4d609a5fb09471813</citedby><cites>FETCH-LOGICAL-c395t-1abd1e2f9e10d0c24d447c1087150b9f08f5729c6fe0c2bf4d609a5fb09471813</cites><orcidid>0000-0002-6457-9731</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0144861713012605$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=28347901$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24607157$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sassaki, Guilherme Lanzi</creatorcontrib><creatorcontrib>Guerrini, Marco</creatorcontrib><creatorcontrib>Serrato, Rodrigo Vassoler</creatorcontrib><creatorcontrib>Santana Filho, Arquimedes Paixão</creatorcontrib><creatorcontrib>Carlotto, Juliane</creatorcontrib><creatorcontrib>Simas-Tosin, Fernanda</creatorcontrib><creatorcontrib>Cipriani, Thales Ricardo</creatorcontrib><creatorcontrib>Iacomini, Marcello</creatorcontrib><creatorcontrib>Torri, Giangiacomo</creatorcontrib><creatorcontrib>Gorin, Philip Albert James</creatorcontrib><title>Monosaccharide composition of glycans based on Q-HSQC NMR</title><title>Carbohydrate polymers</title><addtitle>Carbohydr Polym</addtitle><description>•A method to determine the monosaccharide composition based on NMR was developed.•Q-HSQC depends of a JCH value of 155Hz for C-1, allowing milli-microM detection.•Analysis of the native and hydrolyzed glycan give insights of the molecular mobility.
Glycans have essential functions related to structural architecture and specific cell surface phenomena, such as differentiation, biosignalling, recognition and cell–cell interaction, with the carbohydrate structure determining main function in the cell. Due to the importance of the primary structure, the monosaccharide composition is crucial to show the glycan structure. We now present a method for complex carbohydrates based on NMR spectroscopy, which has shown to give similar results to those obtained by the classic GC–MS-carboxy-reduction/deuterium labeling approach. Quantitative HSQC, through JCH dependence showed 155Hz as the best value for 1H/13C anomeric aldoses, allowing milli-microM detection using conventional inverse probe heads. Combining the quantification of native monosaccharide units of the glycan and those from the hydrolyzed product, a strong correlation occurs between the molecular mobility of the monosaccharide units, giving rise to some insights on the dynamic properties of the parent glycan.</description><subject>Applied sciences</subject><subject>Carbohydrate quantification</subject><subject>Dynamics</subject><subject>Exact sciences and technology</subject><subject>Glycoconjugates</subject><subject>Magnetic Resonance Spectroscopy - methods</subject><subject>Monosaccharides - analysis</subject><subject>Mutarotation</subject><subject>Natural polymers</subject><subject>Physicochemistry of polymers</subject><subject>Polysaccharides</subject><subject>Polysaccharides - chemistry</subject><subject>Starch and polysaccharides</subject><issn>0144-8617</issn><issn>1879-1344</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMlOwzAQQC0EgrJ8AigXJC4JM4njxCeEKjaJRWxny_ECrtK42C0Sf4-rFjgyl5Fm3ix6hBwiFAjITieFkqGb-b4oAasCywIo2yAjbBueY0XpJhkBUpq3DJsdshvjBFIwhG2yU1IGDdbNiPA7P_golXqXwWmTKT-d-ejmzg-Zt9lb_6XkELNORqOzVHvMr58fx9n93dM-2bKyj-ZgnffI6-XFy_g6v324uhmf3-aq4vU8R9lpNKXlBkGDKqmmtFEIbboPHbfQ2ropuWLWpG5nqWbAZW074LTBFqs9crLaOwv-Y2HiXExdVKbv5WD8Ioq0htEEAktovUJV8DEGY8UsuKkMXwJBLK2JiVhbE0trAkuRrKW5o_WJRTc1-nfqR1MCjteAjEr2NshBufjHtRVtOCx_PVtxJgn5dCaIqJwZlNEuGDUX2rt_XvkGZDKLKg</recordid><startdate>20140415</startdate><enddate>20140415</enddate><creator>Sassaki, Guilherme Lanzi</creator><creator>Guerrini, Marco</creator><creator>Serrato, Rodrigo Vassoler</creator><creator>Santana Filho, Arquimedes Paixão</creator><creator>Carlotto, Juliane</creator><creator>Simas-Tosin, Fernanda</creator><creator>Cipriani, Thales Ricardo</creator><creator>Iacomini, Marcello</creator><creator>Torri, Giangiacomo</creator><creator>Gorin, Philip Albert James</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-6457-9731</orcidid></search><sort><creationdate>20140415</creationdate><title>Monosaccharide composition of glycans based on Q-HSQC NMR</title><author>Sassaki, Guilherme Lanzi ; Guerrini, Marco ; Serrato, Rodrigo Vassoler ; Santana Filho, Arquimedes Paixão ; Carlotto, Juliane ; Simas-Tosin, Fernanda ; Cipriani, Thales Ricardo ; Iacomini, Marcello ; Torri, Giangiacomo ; Gorin, Philip Albert James</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c395t-1abd1e2f9e10d0c24d447c1087150b9f08f5729c6fe0c2bf4d609a5fb09471813</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Applied sciences</topic><topic>Carbohydrate quantification</topic><topic>Dynamics</topic><topic>Exact sciences and technology</topic><topic>Glycoconjugates</topic><topic>Magnetic Resonance Spectroscopy - methods</topic><topic>Monosaccharides - analysis</topic><topic>Mutarotation</topic><topic>Natural polymers</topic><topic>Physicochemistry of polymers</topic><topic>Polysaccharides</topic><topic>Polysaccharides - chemistry</topic><topic>Starch and polysaccharides</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sassaki, Guilherme Lanzi</creatorcontrib><creatorcontrib>Guerrini, Marco</creatorcontrib><creatorcontrib>Serrato, Rodrigo Vassoler</creatorcontrib><creatorcontrib>Santana Filho, Arquimedes Paixão</creatorcontrib><creatorcontrib>Carlotto, Juliane</creatorcontrib><creatorcontrib>Simas-Tosin, Fernanda</creatorcontrib><creatorcontrib>Cipriani, Thales Ricardo</creatorcontrib><creatorcontrib>Iacomini, Marcello</creatorcontrib><creatorcontrib>Torri, Giangiacomo</creatorcontrib><creatorcontrib>Gorin, Philip Albert James</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Carbohydrate polymers</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sassaki, Guilherme Lanzi</au><au>Guerrini, Marco</au><au>Serrato, Rodrigo Vassoler</au><au>Santana Filho, Arquimedes Paixão</au><au>Carlotto, Juliane</au><au>Simas-Tosin, Fernanda</au><au>Cipriani, Thales Ricardo</au><au>Iacomini, Marcello</au><au>Torri, Giangiacomo</au><au>Gorin, Philip Albert James</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Monosaccharide composition of glycans based on Q-HSQC NMR</atitle><jtitle>Carbohydrate polymers</jtitle><addtitle>Carbohydr Polym</addtitle><date>2014-04-15</date><risdate>2014</risdate><volume>104</volume><spage>34</spage><epage>41</epage><pages>34-41</pages><issn>0144-8617</issn><eissn>1879-1344</eissn><coden>CAPOD8</coden><abstract>•A method to determine the monosaccharide composition based on NMR was developed.•Q-HSQC depends of a JCH value of 155Hz for C-1, allowing milli-microM detection.•Analysis of the native and hydrolyzed glycan give insights of the molecular mobility.
Glycans have essential functions related to structural architecture and specific cell surface phenomena, such as differentiation, biosignalling, recognition and cell–cell interaction, with the carbohydrate structure determining main function in the cell. Due to the importance of the primary structure, the monosaccharide composition is crucial to show the glycan structure. We now present a method for complex carbohydrates based on NMR spectroscopy, which has shown to give similar results to those obtained by the classic GC–MS-carboxy-reduction/deuterium labeling approach. Quantitative HSQC, through JCH dependence showed 155Hz as the best value for 1H/13C anomeric aldoses, allowing milli-microM detection using conventional inverse probe heads. Combining the quantification of native monosaccharide units of the glycan and those from the hydrolyzed product, a strong correlation occurs between the molecular mobility of the monosaccharide units, giving rise to some insights on the dynamic properties of the parent glycan.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>24607157</pmid><doi>10.1016/j.carbpol.2013.12.046</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-6457-9731</orcidid></addata></record> |
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| subjects | Applied sciences Carbohydrate quantification Dynamics Exact sciences and technology Glycoconjugates Magnetic Resonance Spectroscopy - methods Monosaccharides - analysis Mutarotation Natural polymers Physicochemistry of polymers Polysaccharides Polysaccharides - chemistry Starch and polysaccharides |
| title | Monosaccharide composition of glycans based on Q-HSQC NMR |
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