Immuno-Chromatographic Wicking Assay for the Rapid Detection of Dengue Viral Antigens in Mosquitoes (Diptera: Culicidae)
There is a threat for dengue virus (DENV) reemergence in many regions of the world, particularly in areas where the DENV vectors, Aedes aegypti (L.) and Aedes albopictus (Skuse), are readily available. However, there are currently no accurate and reliable diagnostic methods to provide critical, real...
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| Veröffentlicht in: | Journal of medical entomology 2014-01, Vol.51 (1), p.220-225 |
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| container_title | Journal of medical entomology |
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| creator | Wanja, Elizabeth Parker, Zahra F. Odusami, Oluwakemi Rowland, Tobin Davé, Kirti Davé, Sonia Turell, Michael J. |
| description | There is a threat for dengue virus (DENV) reemergence in many regions of the world, particularly in areas where the DENV vectors, Aedes aegypti (L.) and Aedes albopictus (Skuse), are readily available. However, there are currently no accurate and reliable diagnostic methods to provide critical, real-time information for early detection of DENV within the vector populations to implement appropriate vector control and personal protective measures. In this article, we report the ability of an immuno-chromatographic assay developed by VecTOR Test Systems Inc. to detect DENV in a pool of female Aedes mosquitoes infected with any of the four viral serotypes. The DENV dipstick assay was simple to use, did not require a cold chain, and provided clear results within 30 min. It was highly specific and did not cross-react with samples spiked with West Nile, yellow fever, Japanese encephalitis, Rift Valley fever, chikungunya, Venezuelan equine encephalomyelitis, Ross River, LaCrosse, or Caraparu viruses. The DENV assay can provide real-time critical information on the presence of DENV in mosquitoes to public health personnel. Results from this assay will allow a rapid threat assessment and the focusing of vector control measures in high-risk areas. |
| doi_str_mv | 10.1603/ME12261 |
| format | Article |
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However, there are currently no accurate and reliable diagnostic methods to provide critical, real-time information for early detection of DENV within the vector populations to implement appropriate vector control and personal protective measures. In this article, we report the ability of an immuno-chromatographic assay developed by VecTOR Test Systems Inc. to detect DENV in a pool of female Aedes mosquitoes infected with any of the four viral serotypes. The DENV dipstick assay was simple to use, did not require a cold chain, and provided clear results within 30 min. It was highly specific and did not cross-react with samples spiked with West Nile, yellow fever, Japanese encephalitis, Rift Valley fever, chikungunya, Venezuelan equine encephalomyelitis, Ross River, LaCrosse, or Caraparu viruses. The DENV assay can provide real-time critical information on the presence of DENV in mosquitoes to public health personnel. Results from this assay will allow a rapid threat assessment and the focusing of vector control measures in high-risk areas.</description><identifier>ISSN: 0022-2585</identifier><identifier>EISSN: 1938-2928</identifier><identifier>DOI: 10.1603/ME12261</identifier><identifier>PMID: 24605472</identifier><language>eng</language><publisher>England: Entomological Society of America</publisher><subject>Aedes - virology ; Aedes aegypti ; Aedes albopictus ; Animals ; arbovirus ; Asian tiger mosquito ; Chromatography ; cold ; control methods ; dengue ; Dengue fever ; Dengue virus ; Dengue Virus - isolation & purification ; diagnostic techniques ; dipstick ; Female ; females ; health care workers ; immunoaffinity chromatography ; Immunologic Techniques ; Insect Vectors - virology ; Mosquitoes ; population ; public health ; rapid detection ; rapid methods ; Rift Valley fever ; rivers ; Sensitivity and Specificity ; serotypes ; surveillance ; vector control ; VECTOR-BORNE DISEASES, SURVEILLANCE, PREVENTION ; Venezuelan equine encephalomyelitis ; Viral antigens ; viruses ; wicking assay ; Yellow fever virus</subject><ispartof>Journal of medical entomology, 2014-01, Vol.51 (1), p.220-225</ispartof><rights>COPYRIGHT 2014 Oxford University Press</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b435t-42c4b87c95f69a4793a42cf50517f150f90f6787e25b223e2942fe3c28657ecd3</citedby><cites>FETCH-LOGICAL-b435t-42c4b87c95f69a4793a42cf50517f150f90f6787e25b223e2942fe3c28657ecd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24605472$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wanja, Elizabeth</creatorcontrib><creatorcontrib>Parker, Zahra F.</creatorcontrib><creatorcontrib>Odusami, Oluwakemi</creatorcontrib><creatorcontrib>Rowland, Tobin</creatorcontrib><creatorcontrib>Davé, Kirti</creatorcontrib><creatorcontrib>Davé, Sonia</creatorcontrib><creatorcontrib>Turell, Michael J.</creatorcontrib><title>Immuno-Chromatographic Wicking Assay for the Rapid Detection of Dengue Viral Antigens in Mosquitoes (Diptera: Culicidae)</title><title>Journal of medical entomology</title><addtitle>J Med Entomol</addtitle><description>There is a threat for dengue virus (DENV) reemergence in many regions of the world, particularly in areas where the DENV vectors, Aedes aegypti (L.) and Aedes albopictus (Skuse), are readily available. However, there are currently no accurate and reliable diagnostic methods to provide critical, real-time information for early detection of DENV within the vector populations to implement appropriate vector control and personal protective measures. In this article, we report the ability of an immuno-chromatographic assay developed by VecTOR Test Systems Inc. to detect DENV in a pool of female Aedes mosquitoes infected with any of the four viral serotypes. The DENV dipstick assay was simple to use, did not require a cold chain, and provided clear results within 30 min. It was highly specific and did not cross-react with samples spiked with West Nile, yellow fever, Japanese encephalitis, Rift Valley fever, chikungunya, Venezuelan equine encephalomyelitis, Ross River, LaCrosse, or Caraparu viruses. The DENV assay can provide real-time critical information on the presence of DENV in mosquitoes to public health personnel. Results from this assay will allow a rapid threat assessment and the focusing of vector control measures in high-risk areas.</description><subject>Aedes - virology</subject><subject>Aedes aegypti</subject><subject>Aedes albopictus</subject><subject>Animals</subject><subject>arbovirus</subject><subject>Asian tiger mosquito</subject><subject>Chromatography</subject><subject>cold</subject><subject>control methods</subject><subject>dengue</subject><subject>Dengue fever</subject><subject>Dengue virus</subject><subject>Dengue Virus - isolation & purification</subject><subject>diagnostic techniques</subject><subject>dipstick</subject><subject>Female</subject><subject>females</subject><subject>health care workers</subject><subject>immunoaffinity chromatography</subject><subject>Immunologic Techniques</subject><subject>Insect Vectors - virology</subject><subject>Mosquitoes</subject><subject>population</subject><subject>public health</subject><subject>rapid detection</subject><subject>rapid methods</subject><subject>Rift Valley fever</subject><subject>rivers</subject><subject>Sensitivity and Specificity</subject><subject>serotypes</subject><subject>surveillance</subject><subject>vector control</subject><subject>VECTOR-BORNE DISEASES, SURVEILLANCE, PREVENTION</subject><subject>Venezuelan equine encephalomyelitis</subject><subject>Viral antigens</subject><subject>viruses</subject><subject>wicking assay</subject><subject>Yellow fever virus</subject><issn>0022-2585</issn><issn>1938-2928</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kW9r1TAUxoMo7jrFb6ABEeeLzjRt-sd3l7upgw1Bnb4MaXrSe7RNuiQF9-3N6FUQlEBCDr_znMPzEPI0Z6d5xYo3V-c551V-j2zytmgy3vLmPtkwxnnGRSOOyKMQvjPGmrxsH5IjXlZMlDXfkJ8X07RYl-323k0qusGreY-afkP9A-1AtyGoW2qcp3EP9JOasadnEEFHdJY6kz52WIB-Ra9GurURB7CBoqVXLtwsGB0EenKGcwSv3tLdMqLGXsHrx-SBUWOAJ4f3mFy_O_-y-5Bdfnx_sdteZl1ZiJiVXJddU-tWmKpVZd0WKpWMYCKvTS6YaZmp6qYGLjrOC-BtyQ0UmjeVqEH3xTE5WXVn724WCFFOGDSMo7LgliCTRlWmq64T-mJFBzWCRGtc9Erf4XIrCpGsS54l6vQfVDo9TKidBYOp_lfDq7VBexeCByNnj5PytzJn8i49eUgvkc8Ouy7dBP0f7ndcCXi-AkY5qQaPQV5_5iyJMFYkF0QiXq5Ehy7t8t9RvwCpO6al</recordid><startdate>201401</startdate><enddate>201401</enddate><creator>Wanja, Elizabeth</creator><creator>Parker, Zahra F.</creator><creator>Odusami, Oluwakemi</creator><creator>Rowland, Tobin</creator><creator>Davé, Kirti</creator><creator>Davé, Sonia</creator><creator>Turell, Michael J.</creator><general>Entomological Society of America</general><general>Oxford University Press</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201401</creationdate><title>Immuno-Chromatographic Wicking Assay for the Rapid Detection of Dengue Viral Antigens in Mosquitoes (Diptera: Culicidae)</title><author>Wanja, Elizabeth ; Parker, Zahra F. ; Odusami, Oluwakemi ; Rowland, Tobin ; Davé, Kirti ; Davé, Sonia ; Turell, Michael J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b435t-42c4b87c95f69a4793a42cf50517f150f90f6787e25b223e2942fe3c28657ecd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Aedes - virology</topic><topic>Aedes aegypti</topic><topic>Aedes albopictus</topic><topic>Animals</topic><topic>arbovirus</topic><topic>Asian tiger mosquito</topic><topic>Chromatography</topic><topic>cold</topic><topic>control methods</topic><topic>dengue</topic><topic>Dengue fever</topic><topic>Dengue virus</topic><topic>Dengue Virus - isolation & purification</topic><topic>diagnostic techniques</topic><topic>dipstick</topic><topic>Female</topic><topic>females</topic><topic>health care workers</topic><topic>immunoaffinity chromatography</topic><topic>Immunologic Techniques</topic><topic>Insect Vectors - virology</topic><topic>Mosquitoes</topic><topic>population</topic><topic>public health</topic><topic>rapid detection</topic><topic>rapid methods</topic><topic>Rift Valley fever</topic><topic>rivers</topic><topic>Sensitivity and Specificity</topic><topic>serotypes</topic><topic>surveillance</topic><topic>vector control</topic><topic>VECTOR-BORNE DISEASES, SURVEILLANCE, PREVENTION</topic><topic>Venezuelan equine encephalomyelitis</topic><topic>Viral antigens</topic><topic>viruses</topic><topic>wicking assay</topic><topic>Yellow fever virus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wanja, Elizabeth</creatorcontrib><creatorcontrib>Parker, Zahra F.</creatorcontrib><creatorcontrib>Odusami, Oluwakemi</creatorcontrib><creatorcontrib>Rowland, Tobin</creatorcontrib><creatorcontrib>Davé, Kirti</creatorcontrib><creatorcontrib>Davé, Sonia</creatorcontrib><creatorcontrib>Turell, Michael J.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of medical entomology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wanja, Elizabeth</au><au>Parker, Zahra F.</au><au>Odusami, Oluwakemi</au><au>Rowland, Tobin</au><au>Davé, Kirti</au><au>Davé, Sonia</au><au>Turell, Michael J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immuno-Chromatographic Wicking Assay for the Rapid Detection of Dengue Viral Antigens in Mosquitoes (Diptera: Culicidae)</atitle><jtitle>Journal of medical entomology</jtitle><addtitle>J Med Entomol</addtitle><date>2014-01</date><risdate>2014</risdate><volume>51</volume><issue>1</issue><spage>220</spage><epage>225</epage><pages>220-225</pages><issn>0022-2585</issn><eissn>1938-2928</eissn><abstract>There is a threat for dengue virus (DENV) reemergence in many regions of the world, particularly in areas where the DENV vectors, Aedes aegypti (L.) and Aedes albopictus (Skuse), are readily available. However, there are currently no accurate and reliable diagnostic methods to provide critical, real-time information for early detection of DENV within the vector populations to implement appropriate vector control and personal protective measures. In this article, we report the ability of an immuno-chromatographic assay developed by VecTOR Test Systems Inc. to detect DENV in a pool of female Aedes mosquitoes infected with any of the four viral serotypes. The DENV dipstick assay was simple to use, did not require a cold chain, and provided clear results within 30 min. It was highly specific and did not cross-react with samples spiked with West Nile, yellow fever, Japanese encephalitis, Rift Valley fever, chikungunya, Venezuelan equine encephalomyelitis, Ross River, LaCrosse, or Caraparu viruses. The DENV assay can provide real-time critical information on the presence of DENV in mosquitoes to public health personnel. Results from this assay will allow a rapid threat assessment and the focusing of vector control measures in high-risk areas.</abstract><cop>England</cop><pub>Entomological Society of America</pub><pmid>24605472</pmid><doi>10.1603/ME12261</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-2585 |
| ispartof | Journal of medical entomology, 2014-01, Vol.51 (1), p.220-225 |
| issn | 0022-2585 1938-2928 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1506415077 |
| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE |
| subjects | Aedes - virology Aedes aegypti Aedes albopictus Animals arbovirus Asian tiger mosquito Chromatography cold control methods dengue Dengue fever Dengue virus Dengue Virus - isolation & purification diagnostic techniques dipstick Female females health care workers immunoaffinity chromatography Immunologic Techniques Insect Vectors - virology Mosquitoes population public health rapid detection rapid methods Rift Valley fever rivers Sensitivity and Specificity serotypes surveillance vector control VECTOR-BORNE DISEASES, SURVEILLANCE, PREVENTION Venezuelan equine encephalomyelitis Viral antigens viruses wicking assay Yellow fever virus |
| title | Immuno-Chromatographic Wicking Assay for the Rapid Detection of Dengue Viral Antigens in Mosquitoes (Diptera: Culicidae) |
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