New Bone Formation and Microstructure Assessed by Combination of Confocal Laser Scanning Microscopy and Differential Interference Contrast Microscopy
Bone is a mineralized connective tissue that is continuously and microstructurally remodeled. Altered bone formation and microstructure arise in pathological bone conditions such as osteoporosis, osteonecrosis, fracture repair, and Paget disease of bone. A proper and objective assessment of bone for...
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| Veröffentlicht in: | Calcified tissue international 2014-03, Vol.94 (3), p.338-347 |
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| creator | Yang, Xiaohong Qin, Ling Liang, Weiguo Wang, Wen Tan, Jianrong Liang, Peihong Xu, Jiake Li, Siming Cui, Shuliang |
| description | Bone is a mineralized connective tissue that is continuously and microstructurally remodeled. Altered bone formation and microstructure arise in pathological bone conditions such as osteoporosis, osteonecrosis, fracture repair, and Paget disease of bone. A proper and objective assessment of bone formation and microstructure will provide insight into the understanding of bone pathogenesis and remodeling. Here, new bone formation ex vitro and its microstructure were evaluated in in vivo multiple sequential polychrome-labeled samples using confocal laser scanning microscopy (CLSM), which generated clearer and more reliable images of thick bone sections than conventional fluorescence microscopy (CFM). Intriguingly, fine details of the bone microstructural features, including the mineralization fronts, quiescent versus active osteons, and Volkmann’s channel, were elucidated using CLSM, which defines the relationship between morphological changes and function, when combined with differential interference contrast microscopy. Furthermore, CLSM provided objective evaluations of bone formation, such as the ratio of labeled areas of new bone formation in a rabbit model when compared with CFM. Altogether, new bone formation and its microstructure can be evaluated more adequately using a combination of CLSM and DIC microscopies. |
| doi_str_mv | 10.1007/s00223-013-9815-6 |
| format | Article |
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Altered bone formation and microstructure arise in pathological bone conditions such as osteoporosis, osteonecrosis, fracture repair, and Paget disease of bone. A proper and objective assessment of bone formation and microstructure will provide insight into the understanding of bone pathogenesis and remodeling. Here, new bone formation ex vitro and its microstructure were evaluated in in vivo multiple sequential polychrome-labeled samples using confocal laser scanning microscopy (CLSM), which generated clearer and more reliable images of thick bone sections than conventional fluorescence microscopy (CFM). Intriguingly, fine details of the bone microstructural features, including the mineralization fronts, quiescent versus active osteons, and Volkmann’s channel, were elucidated using CLSM, which defines the relationship between morphological changes and function, when combined with differential interference contrast microscopy. Furthermore, CLSM provided objective evaluations of bone formation, such as the ratio of labeled areas of new bone formation in a rabbit model when compared with CFM. Altogether, new bone formation and its microstructure can be evaluated more adequately using a combination of CLSM and DIC microscopies.</description><identifier>ISSN: 0171-967X</identifier><identifier>EISSN: 1432-0827</identifier><identifier>DOI: 10.1007/s00223-013-9815-6</identifier><identifier>PMID: 24253488</identifier><language>eng</language><publisher>Boston: Springer US</publisher><subject>Animal models ; Animals ; Biochemistry ; Biomedical and Life Sciences ; Bone and Bones - cytology ; Bone and Bones - metabolism ; Bone density ; Bone diseases ; Cell Biology ; Endocrinology ; Female ; Goats ; Life Sciences ; Microscopy ; Microscopy, Confocal - methods ; Microscopy, Interference ; Microstructure ; Original Research ; Orthopedics ; Osteogenesis - physiology ; Pathogenesis</subject><ispartof>Calcified tissue international, 2014-03, Vol.94 (3), p.338-347</ispartof><rights>Springer Science+Business Media New York 2013</rights><rights>Springer Science+Business Media New York 2014</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c405t-da65ed0e249845e074d251cb0c1370c647cf140464da1589de1ad1b51ec39e993</citedby><cites>FETCH-LOGICAL-c405t-da65ed0e249845e074d251cb0c1370c647cf140464da1589de1ad1b51ec39e993</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00223-013-9815-6$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00223-013-9815-6$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24253488$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yang, Xiaohong</creatorcontrib><creatorcontrib>Qin, Ling</creatorcontrib><creatorcontrib>Liang, Weiguo</creatorcontrib><creatorcontrib>Wang, Wen</creatorcontrib><creatorcontrib>Tan, Jianrong</creatorcontrib><creatorcontrib>Liang, Peihong</creatorcontrib><creatorcontrib>Xu, Jiake</creatorcontrib><creatorcontrib>Li, Siming</creatorcontrib><creatorcontrib>Cui, Shuliang</creatorcontrib><title>New Bone Formation and Microstructure Assessed by Combination of Confocal Laser Scanning Microscopy and Differential Interference Contrast Microscopy</title><title>Calcified tissue international</title><addtitle>Calcif Tissue Int</addtitle><addtitle>Calcif Tissue Int</addtitle><description>Bone is a mineralized connective tissue that is continuously and microstructurally remodeled. Altered bone formation and microstructure arise in pathological bone conditions such as osteoporosis, osteonecrosis, fracture repair, and Paget disease of bone. A proper and objective assessment of bone formation and microstructure will provide insight into the understanding of bone pathogenesis and remodeling. Here, new bone formation ex vitro and its microstructure were evaluated in in vivo multiple sequential polychrome-labeled samples using confocal laser scanning microscopy (CLSM), which generated clearer and more reliable images of thick bone sections than conventional fluorescence microscopy (CFM). Intriguingly, fine details of the bone microstructural features, including the mineralization fronts, quiescent versus active osteons, and Volkmann’s channel, were elucidated using CLSM, which defines the relationship between morphological changes and function, when combined with differential interference contrast microscopy. Furthermore, CLSM provided objective evaluations of bone formation, such as the ratio of labeled areas of new bone formation in a rabbit model when compared with CFM. Altogether, new bone formation and its microstructure can be evaluated more adequately using a combination of CLSM and DIC microscopies.</description><subject>Animal models</subject><subject>Animals</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Bone and Bones - cytology</subject><subject>Bone and Bones - metabolism</subject><subject>Bone density</subject><subject>Bone diseases</subject><subject>Cell Biology</subject><subject>Endocrinology</subject><subject>Female</subject><subject>Goats</subject><subject>Life Sciences</subject><subject>Microscopy</subject><subject>Microscopy, Confocal - methods</subject><subject>Microscopy, Interference</subject><subject>Microstructure</subject><subject>Original Research</subject><subject>Orthopedics</subject><subject>Osteogenesis - physiology</subject><subject>Pathogenesis</subject><issn>0171-967X</issn><issn>1432-0827</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkVGL1DAQx4Mo3nr6AXyRgC--VGfSJG0ez9XTg1UfVPCtpOn06LFN1qRF9oP4fU2vqxyCCIEw5De_MPNn7CnCSwSoXiUAIcoCsCxMjarQ99gGZSkKqEV1n20AKyyMrr6dsUcp3QCg1Fo_ZGdCClXKut6wnx_pB38dPPHLEEc7DcFz6zv-YXAxpCnObpoj8YuUKJ-Ot0e-DWM7-BUNfS59H5zd851NFPlnZ70f_PXJ4MLheCt8M_Q9RfLTkNErP1G8LR0tginaNN3peMwe9Haf6MnpPmdfL99-2b4vdp_eXW0vdoWToKais1pRBySkqaUiqGQnFLoWHJYVOC0r16MEqWVnUdWmI7QdtgrJlYaMKc_Zi9V7iOH7TGlqxiE52u-tpzCnBhXkPRnU8H9UGpN3X2mV0ed_oTdhjj4PslBa1EZjlSlcqWXoFKlvDnEYbTw2CM0Sb7PG2-R4myXeRueeZyfz3I7U_en4nWcGxAqk_OSvKd75-p_WXwjvsUU</recordid><startdate>20140301</startdate><enddate>20140301</enddate><creator>Yang, Xiaohong</creator><creator>Qin, Ling</creator><creator>Liang, Weiguo</creator><creator>Wang, Wen</creator><creator>Tan, Jianrong</creator><creator>Liang, Peihong</creator><creator>Xu, Jiake</creator><creator>Li, Siming</creator><creator>Cui, Shuliang</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20140301</creationdate><title>New Bone Formation and Microstructure Assessed by Combination of Confocal Laser Scanning Microscopy and Differential Interference Contrast Microscopy</title><author>Yang, Xiaohong ; 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Altered bone formation and microstructure arise in pathological bone conditions such as osteoporosis, osteonecrosis, fracture repair, and Paget disease of bone. A proper and objective assessment of bone formation and microstructure will provide insight into the understanding of bone pathogenesis and remodeling. Here, new bone formation ex vitro and its microstructure were evaluated in in vivo multiple sequential polychrome-labeled samples using confocal laser scanning microscopy (CLSM), which generated clearer and more reliable images of thick bone sections than conventional fluorescence microscopy (CFM). Intriguingly, fine details of the bone microstructural features, including the mineralization fronts, quiescent versus active osteons, and Volkmann’s channel, were elucidated using CLSM, which defines the relationship between morphological changes and function, when combined with differential interference contrast microscopy. Furthermore, CLSM provided objective evaluations of bone formation, such as the ratio of labeled areas of new bone formation in a rabbit model when compared with CFM. Altogether, new bone formation and its microstructure can be evaluated more adequately using a combination of CLSM and DIC microscopies.</abstract><cop>Boston</cop><pub>Springer US</pub><pmid>24253488</pmid><doi>10.1007/s00223-013-9815-6</doi><tpages>10</tpages></addata></record> |
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| subjects | Animal models Animals Biochemistry Biomedical and Life Sciences Bone and Bones - cytology Bone and Bones - metabolism Bone density Bone diseases Cell Biology Endocrinology Female Goats Life Sciences Microscopy Microscopy, Confocal - methods Microscopy, Interference Microstructure Original Research Orthopedics Osteogenesis - physiology Pathogenesis |
| title | New Bone Formation and Microstructure Assessed by Combination of Confocal Laser Scanning Microscopy and Differential Interference Contrast Microscopy |
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