Sulfated polysaccharide of Caesalpinia ferrea inhibits herpes simplex virus and poliovirus
Herpes simplex virus (HSV) is one of the most regular human pathogens, being a public health problem, and causal agent of several diseases. Poliovirus (PV) is an enteric virus and about 1% of infected individuals develop paralytic poliomyelitis due to viral invasion of the central nervous system and...
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| Veröffentlicht in: | International journal of biological macromolecules 2013-09, Vol.60, p.93-99 |
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| container_title | International journal of biological macromolecules |
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| creator | Lopes, Nayara Faccin-Galhardi, Lígia Carla Espada, Samantha Fernandes Pacheco, Arcelina Cunha Ricardo, Nágila Maria Pontes Silva Linhares, Rosa Elisa Carvalho Nozawa, Carlos |
| description | Herpes simplex virus (HSV) is one of the most regular human pathogens, being a public health problem, and causal agent of several diseases. Poliovirus (PV) is an enteric virus and about 1% of infected individuals develop paralytic poliomyelitis due to viral invasion of the central nervous system and destruction of motor neurons. This work evaluated the activity of a sulfated polysaccharide of Caesalpinia ferrea (SPLCf) in HSV and PV replication. The antiviral effect of SPLCf at varying concentrations was tested by plaque assay under several protocols, such as time-of-addition, adsorption and penetration inhibition and virucidal. Syntheses of viral protein and nucleic acid were also monitored by the immunofluorescence assay and PCR. The SPLCf inhibited virus adsorption and steps after penetration, and inhibited the synthesis of viral protein. Virucidal effect was also shown and nucleic acid synthesis was concurrent with positive results. Our findings suggested that the substance with low toxicity represent a potential viral inhibitor. |
| doi_str_mv | 10.1016/j.ijbiomac.2013.05.015 |
| format | Article |
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Poliovirus (PV) is an enteric virus and about 1% of infected individuals develop paralytic poliomyelitis due to viral invasion of the central nervous system and destruction of motor neurons. This work evaluated the activity of a sulfated polysaccharide of Caesalpinia ferrea (SPLCf) in HSV and PV replication. The antiviral effect of SPLCf at varying concentrations was tested by plaque assay under several protocols, such as time-of-addition, adsorption and penetration inhibition and virucidal. Syntheses of viral protein and nucleic acid were also monitored by the immunofluorescence assay and PCR. The SPLCf inhibited virus adsorption and steps after penetration, and inhibited the synthesis of viral protein. Virucidal effect was also shown and nucleic acid synthesis was concurrent with positive results. Our findings suggested that the substance with low toxicity represent a potential viral inhibitor.</description><identifier>ISSN: 0141-8130</identifier><identifier>EISSN: 1879-0003</identifier><identifier>DOI: 10.1016/j.ijbiomac.2013.05.015</identifier><identifier>PMID: 23707733</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>adsorption ; Antiviral ; Antiviral Agents - chemistry ; Antiviral Agents - pharmacology ; Antiviral Agents - toxicity ; antiviral properties ; Caesalpinia ; Caesalpinia - chemistry ; Caesalpinia ferrea ; Cell Line ; central nervous system ; Enterovirus C ; fluorescent antibody technique ; herpes simplex ; Herpes simplex virus ; Herpesvirus ; Humans ; motor neurons ; Nuclear Magnetic Resonance, Biomolecular ; nucleic acids ; pathogens ; Plant Extracts - chemistry ; Poliovirus ; Poliovirus - drug effects ; polymerase chain reaction ; polysaccharides ; Polysaccharides - chemistry ; Polysaccharides - pharmacology ; Polysaccharides - toxicity ; Simplexvirus - drug effects ; Spectroscopy, Fourier Transform Infrared ; Sulfated polysaccharide ; toxicity ; Viral Plaque Assay ; Virus Replication - drug effects ; viruses</subject><ispartof>International journal of biological macromolecules, 2013-09, Vol.60, p.93-99</ispartof><rights>2013 Elsevier B.V.</rights><rights>Copyright © 2013 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c473t-75262db22d19d6adfcfd1dd6457fa370e153635c701246c4c40dfdef576f7de63</citedby><cites>FETCH-LOGICAL-c473t-75262db22d19d6adfcfd1dd6457fa370e153635c701246c4c40dfdef576f7de63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ijbiomac.2013.05.015$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23707733$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lopes, Nayara</creatorcontrib><creatorcontrib>Faccin-Galhardi, Lígia Carla</creatorcontrib><creatorcontrib>Espada, Samantha Fernandes</creatorcontrib><creatorcontrib>Pacheco, Arcelina Cunha</creatorcontrib><creatorcontrib>Ricardo, Nágila Maria Pontes Silva</creatorcontrib><creatorcontrib>Linhares, Rosa Elisa Carvalho</creatorcontrib><creatorcontrib>Nozawa, Carlos</creatorcontrib><title>Sulfated polysaccharide of Caesalpinia ferrea inhibits herpes simplex virus and poliovirus</title><title>International journal of biological macromolecules</title><addtitle>Int J Biol Macromol</addtitle><description>Herpes simplex virus (HSV) is one of the most regular human pathogens, being a public health problem, and causal agent of several diseases. Poliovirus (PV) is an enteric virus and about 1% of infected individuals develop paralytic poliomyelitis due to viral invasion of the central nervous system and destruction of motor neurons. This work evaluated the activity of a sulfated polysaccharide of Caesalpinia ferrea (SPLCf) in HSV and PV replication. The antiviral effect of SPLCf at varying concentrations was tested by plaque assay under several protocols, such as time-of-addition, adsorption and penetration inhibition and virucidal. Syntheses of viral protein and nucleic acid were also monitored by the immunofluorescence assay and PCR. The SPLCf inhibited virus adsorption and steps after penetration, and inhibited the synthesis of viral protein. Virucidal effect was also shown and nucleic acid synthesis was concurrent with positive results. Our findings suggested that the substance with low toxicity represent a potential viral inhibitor.</description><subject>adsorption</subject><subject>Antiviral</subject><subject>Antiviral Agents - chemistry</subject><subject>Antiviral Agents - pharmacology</subject><subject>Antiviral Agents - toxicity</subject><subject>antiviral properties</subject><subject>Caesalpinia</subject><subject>Caesalpinia - chemistry</subject><subject>Caesalpinia ferrea</subject><subject>Cell Line</subject><subject>central nervous system</subject><subject>Enterovirus C</subject><subject>fluorescent antibody technique</subject><subject>herpes simplex</subject><subject>Herpes simplex virus</subject><subject>Herpesvirus</subject><subject>Humans</subject><subject>motor neurons</subject><subject>Nuclear Magnetic Resonance, Biomolecular</subject><subject>nucleic acids</subject><subject>pathogens</subject><subject>Plant Extracts - chemistry</subject><subject>Poliovirus</subject><subject>Poliovirus - drug effects</subject><subject>polymerase chain reaction</subject><subject>polysaccharides</subject><subject>Polysaccharides - chemistry</subject><subject>Polysaccharides - pharmacology</subject><subject>Polysaccharides - toxicity</subject><subject>Simplexvirus - drug effects</subject><subject>Spectroscopy, Fourier Transform Infrared</subject><subject>Sulfated polysaccharide</subject><subject>toxicity</subject><subject>Viral Plaque Assay</subject><subject>Virus Replication - drug effects</subject><subject>viruses</subject><issn>0141-8130</issn><issn>1879-0003</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkLFu2zAQhomgQeK6fYWUYxeppCiS9tbCaJICBjIkWbIQNHmsz5BElZSM-u0jx07XLjzw8N1_h4-QG85Kzrj6titxt8HYWldWjIuSyZJxeUFmfKGXBWNMfCAzxmteLLhg1-RjzrupqyRfXJHrSmimtRAz8vI4NsEO4Gkfm0O2zm1tQg80BrqykG3TY4eWBkgJLMVuixscMt1C6iHTjG3fwF-6xzRmaru3GIxv30_kMtgmw-dznZPn259Pq_ti_XD3a_VjXbhai6HQslKV31SV50uvrA8ueO69qqUOdroTuBRKSKcZr2rlalczHzwEqVXQHpSYk6-n3D7FPyPkwbSYHTSN7SCO2XDJpKgXx3dO1Al1KeacIJg-YWvTwXBmjl7Nzrx7NUevhkkzeZ0Gb847xk0L_t_Yu8gJ-HICgo3G_k6YzfPjlKAm6XJZV0fi-4mAycUeIZnsEDoHHhO4wfiI_7viFXkml2E</recordid><startdate>20130901</startdate><enddate>20130901</enddate><creator>Lopes, Nayara</creator><creator>Faccin-Galhardi, Lígia Carla</creator><creator>Espada, Samantha Fernandes</creator><creator>Pacheco, Arcelina Cunha</creator><creator>Ricardo, Nágila Maria Pontes Silva</creator><creator>Linhares, Rosa Elisa Carvalho</creator><creator>Nozawa, Carlos</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T2</scope><scope>7U2</scope><scope>7U9</scope><scope>C1K</scope><scope>H94</scope></search><sort><creationdate>20130901</creationdate><title>Sulfated polysaccharide of Caesalpinia ferrea inhibits herpes simplex virus and poliovirus</title><author>Lopes, Nayara ; Faccin-Galhardi, Lígia Carla ; Espada, Samantha Fernandes ; Pacheco, Arcelina Cunha ; Ricardo, Nágila Maria Pontes Silva ; Linhares, Rosa Elisa Carvalho ; Nozawa, Carlos</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c473t-75262db22d19d6adfcfd1dd6457fa370e153635c701246c4c40dfdef576f7de63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>adsorption</topic><topic>Antiviral</topic><topic>Antiviral Agents - chemistry</topic><topic>Antiviral Agents - pharmacology</topic><topic>Antiviral Agents - toxicity</topic><topic>antiviral properties</topic><topic>Caesalpinia</topic><topic>Caesalpinia - chemistry</topic><topic>Caesalpinia ferrea</topic><topic>Cell Line</topic><topic>central nervous system</topic><topic>Enterovirus C</topic><topic>fluorescent antibody technique</topic><topic>herpes simplex</topic><topic>Herpes simplex virus</topic><topic>Herpesvirus</topic><topic>Humans</topic><topic>motor neurons</topic><topic>Nuclear Magnetic Resonance, Biomolecular</topic><topic>nucleic acids</topic><topic>pathogens</topic><topic>Plant Extracts - chemistry</topic><topic>Poliovirus</topic><topic>Poliovirus - drug effects</topic><topic>polymerase chain reaction</topic><topic>polysaccharides</topic><topic>Polysaccharides - chemistry</topic><topic>Polysaccharides - pharmacology</topic><topic>Polysaccharides - toxicity</topic><topic>Simplexvirus - drug effects</topic><topic>Spectroscopy, Fourier Transform Infrared</topic><topic>Sulfated polysaccharide</topic><topic>toxicity</topic><topic>Viral Plaque Assay</topic><topic>Virus Replication - drug effects</topic><topic>viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lopes, Nayara</creatorcontrib><creatorcontrib>Faccin-Galhardi, Lígia Carla</creatorcontrib><creatorcontrib>Espada, Samantha Fernandes</creatorcontrib><creatorcontrib>Pacheco, Arcelina Cunha</creatorcontrib><creatorcontrib>Ricardo, Nágila Maria Pontes Silva</creatorcontrib><creatorcontrib>Linhares, Rosa Elisa Carvalho</creatorcontrib><creatorcontrib>Nozawa, Carlos</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Safety Science and Risk</collection><collection>Virology and AIDS Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>International journal of biological macromolecules</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lopes, Nayara</au><au>Faccin-Galhardi, Lígia Carla</au><au>Espada, Samantha Fernandes</au><au>Pacheco, Arcelina Cunha</au><au>Ricardo, Nágila Maria Pontes Silva</au><au>Linhares, Rosa Elisa Carvalho</au><au>Nozawa, Carlos</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sulfated polysaccharide of Caesalpinia ferrea inhibits herpes simplex virus and poliovirus</atitle><jtitle>International journal of biological macromolecules</jtitle><addtitle>Int J Biol Macromol</addtitle><date>2013-09-01</date><risdate>2013</risdate><volume>60</volume><spage>93</spage><epage>99</epage><pages>93-99</pages><issn>0141-8130</issn><eissn>1879-0003</eissn><abstract>Herpes simplex virus (HSV) is one of the most regular human pathogens, being a public health problem, and causal agent of several diseases. 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| subjects | adsorption Antiviral Antiviral Agents - chemistry Antiviral Agents - pharmacology Antiviral Agents - toxicity antiviral properties Caesalpinia Caesalpinia - chemistry Caesalpinia ferrea Cell Line central nervous system Enterovirus C fluorescent antibody technique herpes simplex Herpes simplex virus Herpesvirus Humans motor neurons Nuclear Magnetic Resonance, Biomolecular nucleic acids pathogens Plant Extracts - chemistry Poliovirus Poliovirus - drug effects polymerase chain reaction polysaccharides Polysaccharides - chemistry Polysaccharides - pharmacology Polysaccharides - toxicity Simplexvirus - drug effects Spectroscopy, Fourier Transform Infrared Sulfated polysaccharide toxicity Viral Plaque Assay Virus Replication - drug effects viruses |
| title | Sulfated polysaccharide of Caesalpinia ferrea inhibits herpes simplex virus and poliovirus |
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