Effect of Sfrp5 on Cytokine Release and Insulin Action in Primary Human Adipocytes and Skeletal Muscle Cells: e85906

Effect of Sfrp5 on Cytokine Release and Insulin Action in Primary Human Adipocytes and Skeletal Muscle Cells: e85906

Secreted frizzled-related protein 5 (Sfrp5) is an adipokine with anti-inflammatory and insulin-sensitizing properties in mice. However, the mechanism of Sfrp5 action, especially in humans, is largely unknown. Therefore, cytokine release and insulin signaling were analyzed to investigate the impact o...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:PloS one 2014-01, Vol.9 (1)
Hauptverfasser: Carstensen, Maren, Wiza, Claudia, Rohrig, Karin, Fahlbusch, Pia, Roden, Michael, Herder, Christian, Ouwens, D Margriet
Format: Artikel
Sprache:eng
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page
container_issue 1
container_start_page
container_title PloS one
container_volume 9
creator Carstensen, Maren
Wiza, Claudia
Rohrig, Karin
Fahlbusch, Pia
Roden, Michael
Herder, Christian
Ouwens, D Margriet
description Secreted frizzled-related protein 5 (Sfrp5) is an adipokine with anti-inflammatory and insulin-sensitizing properties in mice. However, the mechanism of Sfrp5 action, especially in humans, is largely unknown. Therefore, cytokine release and insulin signaling were analyzed to investigate the impact of Sfrp5 on inflammation and insulin signaling in primary human adipocytes and skeletal muscle cells (hSkMC). Sfrp5 neither affected interleukin (IL)-6, monocyte chemoattractant protein-1 (MCP-1) and adiponectin release from human adipocytes, nor IL-6 and IL-8 release from hSkMC. In tumor necrosis factor (TNF) alpha -treated adipocytes, Sfrp5 reduced IL-6 release by 49% (p
doi_str_mv 10.1371/journal.pone.0085906
format Article
fullrecord <record><control><sourceid>proquest</sourceid><recordid>TN_cdi_proquest_miscellaneous_1505346576</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1505346576</sourcerecordid><originalsourceid>FETCH-proquest_miscellaneous_15053465763</originalsourceid><addsrcrecordid>eNqVjs1KAzEUhYMgWH_ewMVduumYNCZt3clQaReCWPclpDeQNpOMucli3t5QfAFX53D4PjiMPQreCbkUz6dUczShG1PEjvOVWnN9xWZiLRdzveDyht0SnThXcqX1jJWNc2gLJAd7l0cFKUI_lXT2EeELAxpCMPEIu0g1-AhvtvjGtPaZ_WDyBNs6mLYf_ZjsVJAu-P7c3GICfFSyAaHHEOgV8PLnnl07Ewgf_vKOPb1vvvvtfMzppyKVw-DJNsNETJUOQrW7L1ottfwH-gu3MFWL</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1505346576</pqid></control><display><type>article</type><title>Effect of Sfrp5 on Cytokine Release and Insulin Action in Primary Human Adipocytes and Skeletal Muscle Cells: e85906</title><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Public Library of Science (PLoS)</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Carstensen, Maren ; Wiza, Claudia ; Rohrig, Karin ; Fahlbusch, Pia ; Roden, Michael ; Herder, Christian ; Ouwens, D Margriet</creator><creatorcontrib>Carstensen, Maren ; Wiza, Claudia ; Rohrig, Karin ; Fahlbusch, Pia ; Roden, Michael ; Herder, Christian ; Ouwens, D Margriet</creatorcontrib><description><![CDATA[Secreted frizzled-related protein 5 (Sfrp5) is an adipokine with anti-inflammatory and insulin-sensitizing properties in mice. However, the mechanism of Sfrp5 action, especially in humans, is largely unknown. Therefore, cytokine release and insulin signaling were analyzed to investigate the impact of Sfrp5 on inflammation and insulin signaling in primary human adipocytes and skeletal muscle cells (hSkMC). Sfrp5 neither affected interleukin (IL)-6, monocyte chemoattractant protein-1 (MCP-1) and adiponectin release from human adipocytes, nor IL-6 and IL-8 release from hSkMC. In tumor necrosis factor (TNF) alpha -treated adipocytes, Sfrp5 reduced IL-6 release by 49% (p<0.05), but did not affect MCP-1 and adiponectin release. In MCP-1-treated hSkMC, Sfrp5 did not affect cytokine secretion. In untreated adipocytes, Sfrp5 decreased the insulin-mediated phosphorylation of Akt-Ser473, Akt-Thr308, GSK3 alpha -Ser21 and PRAS40-Thr246 by 34% (p<0.01), 31% (p<0.05), 37% (p<0.05) and 34% (p<0.01), respectively, and the stimulation of glucose uptake by 25% (p<0.05). Incubation with TNF alpha increased the phosphorylation of JNK and NF Kappa B, and impaired insulin signaling. When Sfrp5 and TNF alpha were combined, there was no additional effect on insulin signaling and JNK phosphorylation, but phosphorylation of NF Kappa B was reversed to basal levels. Sfrp5 had no effect on insulin signaling in untreated or in MCP-1 treated hSkMC. Thus, Sfrp5 lowered IL-6 release and NF Kappa B phosphorylation in cytokine-treated human adipocytes, but not under normal conditions, and decreased insulin signaling in untreated human adipocytes. Sfrp5 did not act on hSkMC. Therefore, the cellular actions of Sfrp5 seem to depend on the type of tissue as well as its inflammatory and metabolic state.]]></description><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0085906</identifier><language>eng</language><ispartof>PloS one, 2014-01, Vol.9 (1)</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,864,27924,27925</link.rule.ids></links><search><creatorcontrib>Carstensen, Maren</creatorcontrib><creatorcontrib>Wiza, Claudia</creatorcontrib><creatorcontrib>Rohrig, Karin</creatorcontrib><creatorcontrib>Fahlbusch, Pia</creatorcontrib><creatorcontrib>Roden, Michael</creatorcontrib><creatorcontrib>Herder, Christian</creatorcontrib><creatorcontrib>Ouwens, D Margriet</creatorcontrib><title>Effect of Sfrp5 on Cytokine Release and Insulin Action in Primary Human Adipocytes and Skeletal Muscle Cells: e85906</title><title>PloS one</title><description><![CDATA[Secreted frizzled-related protein 5 (Sfrp5) is an adipokine with anti-inflammatory and insulin-sensitizing properties in mice. However, the mechanism of Sfrp5 action, especially in humans, is largely unknown. Therefore, cytokine release and insulin signaling were analyzed to investigate the impact of Sfrp5 on inflammation and insulin signaling in primary human adipocytes and skeletal muscle cells (hSkMC). Sfrp5 neither affected interleukin (IL)-6, monocyte chemoattractant protein-1 (MCP-1) and adiponectin release from human adipocytes, nor IL-6 and IL-8 release from hSkMC. In tumor necrosis factor (TNF) alpha -treated adipocytes, Sfrp5 reduced IL-6 release by 49% (p<0.05), but did not affect MCP-1 and adiponectin release. In MCP-1-treated hSkMC, Sfrp5 did not affect cytokine secretion. In untreated adipocytes, Sfrp5 decreased the insulin-mediated phosphorylation of Akt-Ser473, Akt-Thr308, GSK3 alpha -Ser21 and PRAS40-Thr246 by 34% (p<0.01), 31% (p<0.05), 37% (p<0.05) and 34% (p<0.01), respectively, and the stimulation of glucose uptake by 25% (p<0.05). Incubation with TNF alpha increased the phosphorylation of JNK and NF Kappa B, and impaired insulin signaling. When Sfrp5 and TNF alpha were combined, there was no additional effect on insulin signaling and JNK phosphorylation, but phosphorylation of NF Kappa B was reversed to basal levels. Sfrp5 had no effect on insulin signaling in untreated or in MCP-1 treated hSkMC. Thus, Sfrp5 lowered IL-6 release and NF Kappa B phosphorylation in cytokine-treated human adipocytes, but not under normal conditions, and decreased insulin signaling in untreated human adipocytes. Sfrp5 did not act on hSkMC. Therefore, the cellular actions of Sfrp5 seem to depend on the type of tissue as well as its inflammatory and metabolic state.]]></description><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNqVjs1KAzEUhYMgWH_ewMVduumYNCZt3clQaReCWPclpDeQNpOMucli3t5QfAFX53D4PjiMPQreCbkUz6dUczShG1PEjvOVWnN9xWZiLRdzveDyht0SnThXcqX1jJWNc2gLJAd7l0cFKUI_lXT2EeELAxpCMPEIu0g1-AhvtvjGtPaZ_WDyBNs6mLYf_ZjsVJAu-P7c3GICfFSyAaHHEOgV8PLnnl07Ewgf_vKOPb1vvvvtfMzppyKVw-DJNsNETJUOQrW7L1ottfwH-gu3MFWL</recordid><startdate>20140101</startdate><enddate>20140101</enddate><creator>Carstensen, Maren</creator><creator>Wiza, Claudia</creator><creator>Rohrig, Karin</creator><creator>Fahlbusch, Pia</creator><creator>Roden, Michael</creator><creator>Herder, Christian</creator><creator>Ouwens, D Margriet</creator><scope>7T5</scope><scope>H94</scope></search><sort><creationdate>20140101</creationdate><title>Effect of Sfrp5 on Cytokine Release and Insulin Action in Primary Human Adipocytes and Skeletal Muscle Cells: e85906</title><author>Carstensen, Maren ; Wiza, Claudia ; Rohrig, Karin ; Fahlbusch, Pia ; Roden, Michael ; Herder, Christian ; Ouwens, D Margriet</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-proquest_miscellaneous_15053465763</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Carstensen, Maren</creatorcontrib><creatorcontrib>Wiza, Claudia</creatorcontrib><creatorcontrib>Rohrig, Karin</creatorcontrib><creatorcontrib>Fahlbusch, Pia</creatorcontrib><creatorcontrib>Roden, Michael</creatorcontrib><creatorcontrib>Herder, Christian</creatorcontrib><creatorcontrib>Ouwens, D Margriet</creatorcontrib><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Carstensen, Maren</au><au>Wiza, Claudia</au><au>Rohrig, Karin</au><au>Fahlbusch, Pia</au><au>Roden, Michael</au><au>Herder, Christian</au><au>Ouwens, D Margriet</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of Sfrp5 on Cytokine Release and Insulin Action in Primary Human Adipocytes and Skeletal Muscle Cells: e85906</atitle><jtitle>PloS one</jtitle><date>2014-01-01</date><risdate>2014</risdate><volume>9</volume><issue>1</issue><eissn>1932-6203</eissn><abstract><![CDATA[Secreted frizzled-related protein 5 (Sfrp5) is an adipokine with anti-inflammatory and insulin-sensitizing properties in mice. However, the mechanism of Sfrp5 action, especially in humans, is largely unknown. Therefore, cytokine release and insulin signaling were analyzed to investigate the impact of Sfrp5 on inflammation and insulin signaling in primary human adipocytes and skeletal muscle cells (hSkMC). Sfrp5 neither affected interleukin (IL)-6, monocyte chemoattractant protein-1 (MCP-1) and adiponectin release from human adipocytes, nor IL-6 and IL-8 release from hSkMC. In tumor necrosis factor (TNF) alpha -treated adipocytes, Sfrp5 reduced IL-6 release by 49% (p<0.05), but did not affect MCP-1 and adiponectin release. In MCP-1-treated hSkMC, Sfrp5 did not affect cytokine secretion. In untreated adipocytes, Sfrp5 decreased the insulin-mediated phosphorylation of Akt-Ser473, Akt-Thr308, GSK3 alpha -Ser21 and PRAS40-Thr246 by 34% (p<0.01), 31% (p<0.05), 37% (p<0.05) and 34% (p<0.01), respectively, and the stimulation of glucose uptake by 25% (p<0.05). Incubation with TNF alpha increased the phosphorylation of JNK and NF Kappa B, and impaired insulin signaling. When Sfrp5 and TNF alpha were combined, there was no additional effect on insulin signaling and JNK phosphorylation, but phosphorylation of NF Kappa B was reversed to basal levels. Sfrp5 had no effect on insulin signaling in untreated or in MCP-1 treated hSkMC. Thus, Sfrp5 lowered IL-6 release and NF Kappa B phosphorylation in cytokine-treated human adipocytes, but not under normal conditions, and decreased insulin signaling in untreated human adipocytes. Sfrp5 did not act on hSkMC. Therefore, the cellular actions of Sfrp5 seem to depend on the type of tissue as well as its inflammatory and metabolic state.]]></abstract><doi>10.1371/journal.pone.0085906</doi></addata></record>
fulltext fulltext
identifier EISSN: 1932-6203
ispartof PloS one, 2014-01, Vol.9 (1)
issn 1932-6203
language eng
recordid cdi_proquest_miscellaneous_1505346576
source DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); PubMed Central; Free Full-Text Journals in Chemistry
title Effect of Sfrp5 on Cytokine Release and Insulin Action in Primary Human Adipocytes and Skeletal Muscle Cells: e85906
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-05T03%3A18%3A21IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effect%20of%20Sfrp5%20on%20Cytokine%20Release%20and%20Insulin%20Action%20in%20Primary%20Human%20Adipocytes%20and%20Skeletal%20Muscle%20Cells:%20e85906&rft.jtitle=PloS%20one&rft.au=Carstensen,%20Maren&rft.date=2014-01-01&rft.volume=9&rft.issue=1&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0085906&rft_dat=%3Cproquest%3E1505346576%3C/proquest%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1505346576&rft_id=info:pmid/&rfr_iscdi=true