Dispersive solid phase extraction combined with ion-pair ultra high-performance liquid chromatography tandem mass spectrometry for quantification of nucleotides in Lactococcus lactis

Analysis of intracellular metabolites in bacteria is of utmost importance for systems biology and at the same time analytically challenging due to the large difference in concentrations, multiple negative charges, and high polarity of these compounds. To challenge this, a method based on dispersive...

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Veröffentlicht in:	Analytical biochemistry 2013-09, Vol.440 (2), p.166-177
Hauptverfasser:	Magdenoska, Olivera, Martinussen, Jan, Thykaer, Jette, Nielsen, Kristian Fog
Format:	Artikel
Sprache:	eng
Schlagworte:	Butylamines - chemistry Charcoal - chemistry Chromatography, High Pressure Liquid - methods Dispersive solid phase extraction Ion-pair reversed phase liquid chromatography Isotope dilution tandem mass spectrometry Lactococcus lactis Lactococcus lactis - chemistry Nucleotides Nucleotides - analysis Nucleotides - chemistry Nucleotides - isolation & purification Reproducibility of Results Solid Phase Extraction - methods Tandem Mass Spectrometry - methods
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