Structural studies of an exopolysaccharide produced by Gluconacetobacter diazotrophicus Pal5
•Bacterial exopolysaccharide isolation and characterization.•Oligosaccharide isolation and structural determination by NMR, ESI-MS and GC–MS.•Biotechnological applications of the nitrogen-fixing endophyte Gluconacetobacter diazotrophicus, strain Pal5. Gluconacetobacter diazotrophicus is a nitrogen-f...
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| Veröffentlicht in: | Carbohydrate polymers 2013-10, Vol.98 (1), p.1153-1159 |
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| creator | Serrato, Rodrigo V. Meneses, Carlos H.S.G. Vidal, Marcia S. Santana-Filho, Arquimedes P. Iacomini, Marcello Sassaki, Guilherme L. Baldani, José I. |
| description | •Bacterial exopolysaccharide isolation and characterization.•Oligosaccharide isolation and structural determination by NMR, ESI-MS and GC–MS.•Biotechnological applications of the nitrogen-fixing endophyte Gluconacetobacter diazotrophicus, strain Pal5.
Gluconacetobacter diazotrophicus is a nitrogen-fixing bacterium that has been found colonizing several plants. This acid-tolerant bacterium produces phytohormones that promote plant growth and is also able to grow in high-sugar concentrations. It has been demonstrated that exopolysaccharides (EPS), which are produced by strain Pal5 of G. diazotrophicus, play an important role in plant infection. We have investigated the structure of the EPS, which was produced by a strain of Pal5 grown in liquid medium containing mannitol as the sole carbon source. The results reveal an EPS with Glc, Gal, Man in a molar ratio of 6:3:1, respectively. NMR spectroscopy and chemical derivatization have revealed that the EPS structure has 4-O-substituted units of β-glucose, 3-O-substituted units of β-galactose and 2-O-substituted units of α-mannose. Glucose and galactose units linked at C6 were also found. The structure proposed herein is different from EPS produced by other species of Gluconacetobacter published to date. |
| doi_str_mv | 10.1016/j.carbpol.2013.07.025 |
| format | Article |
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Gluconacetobacter diazotrophicus is a nitrogen-fixing bacterium that has been found colonizing several plants. This acid-tolerant bacterium produces phytohormones that promote plant growth and is also able to grow in high-sugar concentrations. It has been demonstrated that exopolysaccharides (EPS), which are produced by strain Pal5 of G. diazotrophicus, play an important role in plant infection. We have investigated the structure of the EPS, which was produced by a strain of Pal5 grown in liquid medium containing mannitol as the sole carbon source. The results reveal an EPS with Glc, Gal, Man in a molar ratio of 6:3:1, respectively. NMR spectroscopy and chemical derivatization have revealed that the EPS structure has 4-O-substituted units of β-glucose, 3-O-substituted units of β-galactose and 2-O-substituted units of α-mannose. Glucose and galactose units linked at C6 were also found. The structure proposed herein is different from EPS produced by other species of Gluconacetobacter published to date.</description><identifier>ISSN: 0144-8617</identifier><identifier>EISSN: 1879-1344</identifier><identifier>DOI: 10.1016/j.carbpol.2013.07.025</identifier><identifier>PMID: 23987457</identifier><identifier>CODEN: CAPOD8</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>acid tolerance ; Applied sciences ; Bacteriology ; Biological and medical sciences ; carbon ; derivatization ; Exact sciences and technology ; Exopolysaccharide ; exopolysaccharides ; Fundamental and applied biological sciences. Psychology ; galactose ; Gluconacetobacter ; Gluconacetobacter - metabolism ; Gluconacetobacter - physiology ; Gluconacetobacter diazotrophicus ; glucose ; Glycosylation ; Hydrolysis ; mannitol ; Metabolism. Enzymes ; Microbiology ; Molecular Weight ; Monosaccharides - chemistry ; Natural polymers ; Nitrogen-fixation ; nitrogen-fixing bacteria ; nuclear magnetic resonance spectroscopy ; Physicochemistry of polymers ; plant growth ; plant hormones ; Plants - microbiology ; Plant–microbe interaction ; Polysaccharides, Bacterial - biosynthesis ; Polysaccharides, Bacterial - chemistry ; Starch and polysaccharides</subject><ispartof>Carbohydrate polymers, 2013-10, Vol.98 (1), p.1153-1159</ispartof><rights>2013 Elsevier Ltd</rights><rights>2014 INIST-CNRS</rights><rights>Copyright © 2013 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c518t-3070f855f7fe0964a120faea4e2b20a228af56b5a67048b914b8de70fca984b03</citedby><cites>FETCH-LOGICAL-c518t-3070f855f7fe0964a120faea4e2b20a228af56b5a67048b914b8de70fca984b03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0144861713007091$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=27757209$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23987457$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Serrato, Rodrigo V.</creatorcontrib><creatorcontrib>Meneses, Carlos H.S.G.</creatorcontrib><creatorcontrib>Vidal, Marcia S.</creatorcontrib><creatorcontrib>Santana-Filho, Arquimedes P.</creatorcontrib><creatorcontrib>Iacomini, Marcello</creatorcontrib><creatorcontrib>Sassaki, Guilherme L.</creatorcontrib><creatorcontrib>Baldani, José I.</creatorcontrib><title>Structural studies of an exopolysaccharide produced by Gluconacetobacter diazotrophicus Pal5</title><title>Carbohydrate polymers</title><addtitle>Carbohydr Polym</addtitle><description>•Bacterial exopolysaccharide isolation and characterization.•Oligosaccharide isolation and structural determination by NMR, ESI-MS and GC–MS.•Biotechnological applications of the nitrogen-fixing endophyte Gluconacetobacter diazotrophicus, strain Pal5.
Gluconacetobacter diazotrophicus is a nitrogen-fixing bacterium that has been found colonizing several plants. This acid-tolerant bacterium produces phytohormones that promote plant growth and is also able to grow in high-sugar concentrations. It has been demonstrated that exopolysaccharides (EPS), which are produced by strain Pal5 of G. diazotrophicus, play an important role in plant infection. We have investigated the structure of the EPS, which was produced by a strain of Pal5 grown in liquid medium containing mannitol as the sole carbon source. The results reveal an EPS with Glc, Gal, Man in a molar ratio of 6:3:1, respectively. NMR spectroscopy and chemical derivatization have revealed that the EPS structure has 4-O-substituted units of β-glucose, 3-O-substituted units of β-galactose and 2-O-substituted units of α-mannose. Glucose and galactose units linked at C6 were also found. The structure proposed herein is different from EPS produced by other species of Gluconacetobacter published to date.</description><subject>acid tolerance</subject><subject>Applied sciences</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>carbon</subject><subject>derivatization</subject><subject>Exact sciences and technology</subject><subject>Exopolysaccharide</subject><subject>exopolysaccharides</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>galactose</subject><subject>Gluconacetobacter</subject><subject>Gluconacetobacter - metabolism</subject><subject>Gluconacetobacter - physiology</subject><subject>Gluconacetobacter diazotrophicus</subject><subject>glucose</subject><subject>Glycosylation</subject><subject>Hydrolysis</subject><subject>mannitol</subject><subject>Metabolism. Enzymes</subject><subject>Microbiology</subject><subject>Molecular Weight</subject><subject>Monosaccharides - chemistry</subject><subject>Natural polymers</subject><subject>Nitrogen-fixation</subject><subject>nitrogen-fixing bacteria</subject><subject>nuclear magnetic resonance spectroscopy</subject><subject>Physicochemistry of polymers</subject><subject>plant growth</subject><subject>plant hormones</subject><subject>Plants - microbiology</subject><subject>Plant–microbe interaction</subject><subject>Polysaccharides, Bacterial - biosynthesis</subject><subject>Polysaccharides, Bacterial - chemistry</subject><subject>Starch and polysaccharides</subject><issn>0144-8617</issn><issn>1879-1344</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhi0EotvCTwByQeKSZfwVJyeEKmiRKoFUekOyJs6YepWNFztBLL8er3aBY33x5XlnXs3D2AsOaw68ebtZO0z9Lo5rAVyuwaxB6EdsxVvT1Vwq9ZitgCtVtw03Z-w85w2U13B4ys6E7FqjtFmxb7dzWty8JByrPC9DoFxFX-FU0a9Ypu8zOnePKQxU7VIcFkdD1e-rq3FxcUJHc-zRzZSqIeDvOKe4uw9uydUXHPUz9sTjmOn56b9gdx8_fL28rm8-X326fH9TO83buZZgwLdae-MJukYhF-CRUJHoBaAQLXrd9BobA6rtO676dqCScdi1qgd5wd4c55aGPxbKs92G7GgccaK4ZMs1aKnAGPkwqkQneCekKKg-oi7FnBN5u0thi2lvOdiDA7uxJwf24MCCscVByb08rVj6LQ3_Un-PXoDXJwCzw9EnnFzI_zljtBHQFe7VkfMYLX5Phbm7LZua4lFL2RwqvjsSVK77M1Cy2QWaiqOQyM12iOGBsn8AyqSxWw</recordid><startdate>20131015</startdate><enddate>20131015</enddate><creator>Serrato, Rodrigo V.</creator><creator>Meneses, Carlos H.S.G.</creator><creator>Vidal, Marcia S.</creator><creator>Santana-Filho, Arquimedes P.</creator><creator>Iacomini, Marcello</creator><creator>Sassaki, Guilherme L.</creator><creator>Baldani, José I.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>20131015</creationdate><title>Structural studies of an exopolysaccharide produced by Gluconacetobacter diazotrophicus Pal5</title><author>Serrato, Rodrigo V. ; Meneses, Carlos H.S.G. ; Vidal, Marcia S. ; Santana-Filho, Arquimedes P. ; Iacomini, Marcello ; Sassaki, Guilherme L. ; Baldani, José I.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c518t-3070f855f7fe0964a120faea4e2b20a228af56b5a67048b914b8de70fca984b03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>acid tolerance</topic><topic>Applied sciences</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>carbon</topic><topic>derivatization</topic><topic>Exact sciences and technology</topic><topic>Exopolysaccharide</topic><topic>exopolysaccharides</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>galactose</topic><topic>Gluconacetobacter</topic><topic>Gluconacetobacter - metabolism</topic><topic>Gluconacetobacter - physiology</topic><topic>Gluconacetobacter diazotrophicus</topic><topic>glucose</topic><topic>Glycosylation</topic><topic>Hydrolysis</topic><topic>mannitol</topic><topic>Metabolism. Enzymes</topic><topic>Microbiology</topic><topic>Molecular Weight</topic><topic>Monosaccharides - chemistry</topic><topic>Natural polymers</topic><topic>Nitrogen-fixation</topic><topic>nitrogen-fixing bacteria</topic><topic>nuclear magnetic resonance spectroscopy</topic><topic>Physicochemistry of polymers</topic><topic>plant growth</topic><topic>plant hormones</topic><topic>Plants - microbiology</topic><topic>Plant–microbe interaction</topic><topic>Polysaccharides, Bacterial - biosynthesis</topic><topic>Polysaccharides, Bacterial - chemistry</topic><topic>Starch and polysaccharides</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Serrato, Rodrigo V.</creatorcontrib><creatorcontrib>Meneses, Carlos H.S.G.</creatorcontrib><creatorcontrib>Vidal, Marcia S.</creatorcontrib><creatorcontrib>Santana-Filho, Arquimedes P.</creatorcontrib><creatorcontrib>Iacomini, Marcello</creatorcontrib><creatorcontrib>Sassaki, Guilherme L.</creatorcontrib><creatorcontrib>Baldani, José I.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Carbohydrate polymers</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Serrato, Rodrigo V.</au><au>Meneses, Carlos H.S.G.</au><au>Vidal, Marcia S.</au><au>Santana-Filho, Arquimedes P.</au><au>Iacomini, Marcello</au><au>Sassaki, Guilherme L.</au><au>Baldani, José I.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Structural studies of an exopolysaccharide produced by Gluconacetobacter diazotrophicus Pal5</atitle><jtitle>Carbohydrate polymers</jtitle><addtitle>Carbohydr Polym</addtitle><date>2013-10-15</date><risdate>2013</risdate><volume>98</volume><issue>1</issue><spage>1153</spage><epage>1159</epage><pages>1153-1159</pages><issn>0144-8617</issn><eissn>1879-1344</eissn><coden>CAPOD8</coden><abstract>•Bacterial exopolysaccharide isolation and characterization.•Oligosaccharide isolation and structural determination by NMR, ESI-MS and GC–MS.•Biotechnological applications of the nitrogen-fixing endophyte Gluconacetobacter diazotrophicus, strain Pal5.
Gluconacetobacter diazotrophicus is a nitrogen-fixing bacterium that has been found colonizing several plants. This acid-tolerant bacterium produces phytohormones that promote plant growth and is also able to grow in high-sugar concentrations. It has been demonstrated that exopolysaccharides (EPS), which are produced by strain Pal5 of G. diazotrophicus, play an important role in plant infection. We have investigated the structure of the EPS, which was produced by a strain of Pal5 grown in liquid medium containing mannitol as the sole carbon source. The results reveal an EPS with Glc, Gal, Man in a molar ratio of 6:3:1, respectively. NMR spectroscopy and chemical derivatization have revealed that the EPS structure has 4-O-substituted units of β-glucose, 3-O-substituted units of β-galactose and 2-O-substituted units of α-mannose. Glucose and galactose units linked at C6 were also found. The structure proposed herein is different from EPS produced by other species of Gluconacetobacter published to date.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>23987457</pmid><doi>10.1016/j.carbpol.2013.07.025</doi><tpages>7</tpages></addata></record> |
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| subjects | acid tolerance Applied sciences Bacteriology Biological and medical sciences carbon derivatization Exact sciences and technology Exopolysaccharide exopolysaccharides Fundamental and applied biological sciences. Psychology galactose Gluconacetobacter Gluconacetobacter - metabolism Gluconacetobacter - physiology Gluconacetobacter diazotrophicus glucose Glycosylation Hydrolysis mannitol Metabolism. Enzymes Microbiology Molecular Weight Monosaccharides - chemistry Natural polymers Nitrogen-fixation nitrogen-fixing bacteria nuclear magnetic resonance spectroscopy Physicochemistry of polymers plant growth plant hormones Plants - microbiology Plant–microbe interaction Polysaccharides, Bacterial - biosynthesis Polysaccharides, Bacterial - chemistry Starch and polysaccharides |
| title | Structural studies of an exopolysaccharide produced by Gluconacetobacter diazotrophicus Pal5 |
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