Isolation and Selection of a Highly Tolerant Microbial Consortium with Potential for PAH Biodegradation from Heavy Crude Oil-Contaminated Soils
A degrading microbial consortium highly tolerant to three-, four- and five-ring polycyclic aromatic hydrocarbons (PAHs) was selected from 50 fungal and bacterial isolates obtained from crude oil-contaminated soils. Morphological and molecular studies indicated that isolated fungi belonged to genera...
Gespeichert in:
| Veröffentlicht in: | Water, air, and soil pollution air, and soil pollution, 2014-02, Vol.225 (2), p.1-18, Article 1826 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 18 |
|---|---|
| container_issue | 2 |
| container_start_page | 1 |
| container_title | Water, air, and soil pollution |
| container_volume | 225 |
| creator | Zafra, German Absalón, Ángel E Cuevas, Ma. Del Carmen Cortés-Espinosa, Diana V |
| description | A degrading microbial consortium highly tolerant to three-, four- and five-ring polycyclic aromatic hydrocarbons (PAHs) was selected from 50 fungal and bacterial isolates obtained from crude oil-contaminated soils. Morphological and molecular studies indicated that isolated fungi belonged to genera Aspergillus, Penicillium, Fusarium, Trichoderma, Scedosporium, and Acremonium and bacteria to Pseudomonas, Klebsiella, Bacillus, Enterobacter, Streptomyces, Stenotrophomonas, Kocuria, and Delftia genera. Individual fungal and bacterial isolates were evaluated for their potential to tolerate high concentrations of different molecular weight PAHs, as phenantrene (Phe), pyrene (Pyr), and benzo[a]pyrene (BaP) by surface plate assays, showing significant differences in extension rates for fungi and inhibition ratios for bacteria when both were exposed to 0–6,000 mg of PAHs per liter. Trichoderma asperellum H15, Aspergillus nomius H7, Aspergillus flavus H6, Pseudomonas aeruginosa B7, Klebsiella sp. B10, and Stenotrophomonas maltophilia B14 grew using PAHs as sole carbon source and presented a remarkably high tolerance to PAHs, up to 6,000 mg l⁻¹. The consortium composed of 12 fungal and bacterial PAH-tolerant isolates for the bioremediation of a PAH-contaminated soiled to a removal of 87.76 % Phe, 48.18 % Pyr, and 56.55 % BaP after 14 days. The degrading microbial consortium presented high potential for bioremediation and may be useful for the treatment of sites polluted with PAHs due to their elevated tolerance to high molecular weight (HMW) PAHs and their capacity to utilize them as energy source. This is the first study which evaluated the microbial tolerance to extreme concentrations of PAHs, resulting in a degrading consortium and highly tolerant consortium compared with those reported in other studies, where the concentrations tested are low. |
| doi_str_mv | 10.1007/s11270-013-1826-4 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_1505340464</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A369129764</galeid><sourcerecordid>A369129764</sourcerecordid><originalsourceid>FETCH-LOGICAL-c412t-90e198c3187ed70913a45a2715f8eb83c60fa5d367da683ffb96135bbd97d7933</originalsourceid><addsrcrecordid>eNp9kkFvFCEYhidGE9fqD_AkiRcvU2GAYTiuG3Wb1LRJ2zNhho8pDQMVWM3-Cv-ybMeD8SAcyAfv8_KFl6Z5S_A5wVh8zIR0AreY0JYMXd-yZ82GcEHbTtLuebPBmMm2l0K-bF7l_IDrkIPYNL8ucvS6uBiQDgbdgIfpqYoWabR3870_otvoIelQ0Dc3pTg67dEuhhxTcYcF_XTlHl3HAqGcTmxM6Hq7R59cNDAnbVZ3m-KC9qB_HNEuHQygK-fb6lL04oIuUO-OzufXzQurfYY3f9az5u7L59vdvr28-nqx2162EyNdaSUGIoeJkkGAEVgSqhnXnSDcDjAOdOqx1dzQXhjdD9TaUfaE8nE0UhghKT1rPqy-jyl-P0AuanF5Au91gHjIinDMKcOsZ1X6_h_pQzykULtThEnJxEAprqrzVTVrD8oFG0vSU50GFjfFANbV_S3tJemkeLIlK1CfNOcEVj0mt-h0VASrU6ZqzVTVTNUpU3ViupXJVRtmSH-18h_o3QpZHZWek8vq7qbDhNU_wInknP4GFLOtUQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1499478330</pqid></control><display><type>article</type><title>Isolation and Selection of a Highly Tolerant Microbial Consortium with Potential for PAH Biodegradation from Heavy Crude Oil-Contaminated Soils</title><source>SpringerNature Journals</source><creator>Zafra, German ; Absalón, Ángel E ; Cuevas, Ma. Del Carmen ; Cortés-Espinosa, Diana V</creator><creatorcontrib>Zafra, German ; Absalón, Ángel E ; Cuevas, Ma. Del Carmen ; Cortés-Espinosa, Diana V</creatorcontrib><description>A degrading microbial consortium highly tolerant to three-, four- and five-ring polycyclic aromatic hydrocarbons (PAHs) was selected from 50 fungal and bacterial isolates obtained from crude oil-contaminated soils. Morphological and molecular studies indicated that isolated fungi belonged to genera Aspergillus, Penicillium, Fusarium, Trichoderma, Scedosporium, and Acremonium and bacteria to Pseudomonas, Klebsiella, Bacillus, Enterobacter, Streptomyces, Stenotrophomonas, Kocuria, and Delftia genera. Individual fungal and bacterial isolates were evaluated for their potential to tolerate high concentrations of different molecular weight PAHs, as phenantrene (Phe), pyrene (Pyr), and benzo[a]pyrene (BaP) by surface plate assays, showing significant differences in extension rates for fungi and inhibition ratios for bacteria when both were exposed to 0–6,000 mg of PAHs per liter. Trichoderma asperellum H15, Aspergillus nomius H7, Aspergillus flavus H6, Pseudomonas aeruginosa B7, Klebsiella sp. B10, and Stenotrophomonas maltophilia B14 grew using PAHs as sole carbon source and presented a remarkably high tolerance to PAHs, up to 6,000 mg l⁻¹. The consortium composed of 12 fungal and bacterial PAH-tolerant isolates for the bioremediation of a PAH-contaminated soiled to a removal of 87.76 % Phe, 48.18 % Pyr, and 56.55 % BaP after 14 days. The degrading microbial consortium presented high potential for bioremediation and may be useful for the treatment of sites polluted with PAHs due to their elevated tolerance to high molecular weight (HMW) PAHs and their capacity to utilize them as energy source. This is the first study which evaluated the microbial tolerance to extreme concentrations of PAHs, resulting in a degrading consortium and highly tolerant consortium compared with those reported in other studies, where the concentrations tested are low.</description><identifier>ISSN: 0049-6979</identifier><identifier>EISSN: 1573-2932</identifier><identifier>DOI: 10.1007/s11270-013-1826-4</identifier><language>eng</language><publisher>Cham: Springer-Verlag</publisher><subject>Acremonium ; Aspergillus ; Aspergillus flavus ; Aspergillus nomius ; Atmospheric Protection/Air Quality Control/Air Pollution ; Bacillus ; Bacteria ; Benzo(a)pyrene ; Benzopyrene ; Biodegradation ; Bioremediation ; Carbon sources ; Climate Change/Climate Change Impacts ; Consortia ; Crude oil ; Delftia ; Earth and Environmental Science ; Energy sources ; Enterobacter ; Environment ; Environmental impact ; Environmental monitoring ; Environmental studies ; Enzymes ; Fungi ; Fusarium ; Hydrogeology ; Klebsiella ; Kocuria ; Metabolism ; Microbial contamination ; Microorganisms ; Molecular weight ; Oil ; Oil pollution ; Oxidation ; Penicillium ; Pollutants ; Polycyclic aromatic hydrocarbons ; Pseudomonas aeruginosa ; Pyrene ; Scedosporium ; soil ; Soil contamination ; Soil microorganisms ; Soil pollution ; Soil Science & Conservation ; Soils ; Stenotrophomonas ; Stenotrophomonas maltophilia ; Streptomyces ; Trichoderma ; Trichoderma asperellum ; Water Quality/Water Pollution</subject><ispartof>Water, air, and soil pollution, 2014-02, Vol.225 (2), p.1-18, Article 1826</ispartof><rights>Springer Science+Business Media Dordrecht 2013</rights><rights>COPYRIGHT 2014 Springer</rights><rights>Springer International Publishing Switzerland 2014</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-90e198c3187ed70913a45a2715f8eb83c60fa5d367da683ffb96135bbd97d7933</citedby><cites>FETCH-LOGICAL-c412t-90e198c3187ed70913a45a2715f8eb83c60fa5d367da683ffb96135bbd97d7933</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11270-013-1826-4$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11270-013-1826-4$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Zafra, German</creatorcontrib><creatorcontrib>Absalón, Ángel E</creatorcontrib><creatorcontrib>Cuevas, Ma. Del Carmen</creatorcontrib><creatorcontrib>Cortés-Espinosa, Diana V</creatorcontrib><title>Isolation and Selection of a Highly Tolerant Microbial Consortium with Potential for PAH Biodegradation from Heavy Crude Oil-Contaminated Soils</title><title>Water, air, and soil pollution</title><addtitle>Water Air Soil Pollut</addtitle><description>A degrading microbial consortium highly tolerant to three-, four- and five-ring polycyclic aromatic hydrocarbons (PAHs) was selected from 50 fungal and bacterial isolates obtained from crude oil-contaminated soils. Morphological and molecular studies indicated that isolated fungi belonged to genera Aspergillus, Penicillium, Fusarium, Trichoderma, Scedosporium, and Acremonium and bacteria to Pseudomonas, Klebsiella, Bacillus, Enterobacter, Streptomyces, Stenotrophomonas, Kocuria, and Delftia genera. Individual fungal and bacterial isolates were evaluated for their potential to tolerate high concentrations of different molecular weight PAHs, as phenantrene (Phe), pyrene (Pyr), and benzo[a]pyrene (BaP) by surface plate assays, showing significant differences in extension rates for fungi and inhibition ratios for bacteria when both were exposed to 0–6,000 mg of PAHs per liter. Trichoderma asperellum H15, Aspergillus nomius H7, Aspergillus flavus H6, Pseudomonas aeruginosa B7, Klebsiella sp. B10, and Stenotrophomonas maltophilia B14 grew using PAHs as sole carbon source and presented a remarkably high tolerance to PAHs, up to 6,000 mg l⁻¹. The consortium composed of 12 fungal and bacterial PAH-tolerant isolates for the bioremediation of a PAH-contaminated soiled to a removal of 87.76 % Phe, 48.18 % Pyr, and 56.55 % BaP after 14 days. The degrading microbial consortium presented high potential for bioremediation and may be useful for the treatment of sites polluted with PAHs due to their elevated tolerance to high molecular weight (HMW) PAHs and their capacity to utilize them as energy source. This is the first study which evaluated the microbial tolerance to extreme concentrations of PAHs, resulting in a degrading consortium and highly tolerant consortium compared with those reported in other studies, where the concentrations tested are low.</description><subject>Acremonium</subject><subject>Aspergillus</subject><subject>Aspergillus flavus</subject><subject>Aspergillus nomius</subject><subject>Atmospheric Protection/Air Quality Control/Air Pollution</subject><subject>Bacillus</subject><subject>Bacteria</subject><subject>Benzo(a)pyrene</subject><subject>Benzopyrene</subject><subject>Biodegradation</subject><subject>Bioremediation</subject><subject>Carbon sources</subject><subject>Climate Change/Climate Change Impacts</subject><subject>Consortia</subject><subject>Crude oil</subject><subject>Delftia</subject><subject>Earth and Environmental Science</subject><subject>Energy sources</subject><subject>Enterobacter</subject><subject>Environment</subject><subject>Environmental impact</subject><subject>Environmental monitoring</subject><subject>Environmental studies</subject><subject>Enzymes</subject><subject>Fungi</subject><subject>Fusarium</subject><subject>Hydrogeology</subject><subject>Klebsiella</subject><subject>Kocuria</subject><subject>Metabolism</subject><subject>Microbial contamination</subject><subject>Microorganisms</subject><subject>Molecular weight</subject><subject>Oil</subject><subject>Oil pollution</subject><subject>Oxidation</subject><subject>Penicillium</subject><subject>Pollutants</subject><subject>Polycyclic aromatic hydrocarbons</subject><subject>Pseudomonas aeruginosa</subject><subject>Pyrene</subject><subject>Scedosporium</subject><subject>soil</subject><subject>Soil contamination</subject><subject>Soil microorganisms</subject><subject>Soil pollution</subject><subject>Soil Science & Conservation</subject><subject>Soils</subject><subject>Stenotrophomonas</subject><subject>Stenotrophomonas maltophilia</subject><subject>Streptomyces</subject><subject>Trichoderma</subject><subject>Trichoderma asperellum</subject><subject>Water Quality/Water Pollution</subject><issn>0049-6979</issn><issn>1573-2932</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kkFvFCEYhidGE9fqD_AkiRcvU2GAYTiuG3Wb1LRJ2zNhho8pDQMVWM3-Cv-ybMeD8SAcyAfv8_KFl6Z5S_A5wVh8zIR0AreY0JYMXd-yZ82GcEHbTtLuebPBmMm2l0K-bF7l_IDrkIPYNL8ucvS6uBiQDgbdgIfpqYoWabR3870_otvoIelQ0Dc3pTg67dEuhhxTcYcF_XTlHl3HAqGcTmxM6Hq7R59cNDAnbVZ3m-KC9qB_HNEuHQygK-fb6lL04oIuUO-OzufXzQurfYY3f9az5u7L59vdvr28-nqx2162EyNdaSUGIoeJkkGAEVgSqhnXnSDcDjAOdOqx1dzQXhjdD9TaUfaE8nE0UhghKT1rPqy-jyl-P0AuanF5Au91gHjIinDMKcOsZ1X6_h_pQzykULtThEnJxEAprqrzVTVrD8oFG0vSU50GFjfFANbV_S3tJemkeLIlK1CfNOcEVj0mt-h0VASrU6ZqzVTVTNUpU3ViupXJVRtmSH-18h_o3QpZHZWek8vq7qbDhNU_wInknP4GFLOtUQ</recordid><startdate>20140201</startdate><enddate>20140201</enddate><creator>Zafra, German</creator><creator>Absalón, Ángel E</creator><creator>Cuevas, Ma. Del Carmen</creator><creator>Cortés-Espinosa, Diana V</creator><general>Springer-Verlag</general><general>Springer International Publishing</general><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QH</scope><scope>7T7</scope><scope>7TV</scope><scope>7U7</scope><scope>7UA</scope><scope>7WY</scope><scope>7WZ</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>87Z</scope><scope>88E</scope><scope>88I</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8FL</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BEZIV</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FR3</scope><scope>FRNLG</scope><scope>FYUFA</scope><scope>F~G</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H96</scope><scope>H97</scope><scope>HCIFZ</scope><scope>K60</scope><scope>K6~</scope><scope>K9.</scope><scope>L.-</scope><scope>L.G</scope><scope>M0C</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>P64</scope><scope>PATMY</scope><scope>PCBAR</scope><scope>PQBIZ</scope><scope>PQBZA</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope><scope>Q9U</scope><scope>7QO</scope><scope>7ST</scope><scope>7U6</scope><scope>SOI</scope></search><sort><creationdate>20140201</creationdate><title>Isolation and Selection of a Highly Tolerant Microbial Consortium with Potential for PAH Biodegradation from Heavy Crude Oil-Contaminated Soils</title><author>Zafra, German ; Absalón, Ángel E ; Cuevas, Ma. Del Carmen ; Cortés-Espinosa, Diana V</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c412t-90e198c3187ed70913a45a2715f8eb83c60fa5d367da683ffb96135bbd97d7933</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Acremonium</topic><topic>Aspergillus</topic><topic>Aspergillus flavus</topic><topic>Aspergillus nomius</topic><topic>Atmospheric Protection/Air Quality Control/Air Pollution</topic><topic>Bacillus</topic><topic>Bacteria</topic><topic>Benzo(a)pyrene</topic><topic>Benzopyrene</topic><topic>Biodegradation</topic><topic>Bioremediation</topic><topic>Carbon sources</topic><topic>Climate Change/Climate Change Impacts</topic><topic>Consortia</topic><topic>Crude oil</topic><topic>Delftia</topic><topic>Earth and Environmental Science</topic><topic>Energy sources</topic><topic>Enterobacter</topic><topic>Environment</topic><topic>Environmental impact</topic><topic>Environmental monitoring</topic><topic>Environmental studies</topic><topic>Enzymes</topic><topic>Fungi</topic><topic>Fusarium</topic><topic>Hydrogeology</topic><topic>Klebsiella</topic><topic>Kocuria</topic><topic>Metabolism</topic><topic>Microbial contamination</topic><topic>Microorganisms</topic><topic>Molecular weight</topic><topic>Oil</topic><topic>Oil pollution</topic><topic>Oxidation</topic><topic>Penicillium</topic><topic>Pollutants</topic><topic>Polycyclic aromatic hydrocarbons</topic><topic>Pseudomonas aeruginosa</topic><topic>Pyrene</topic><topic>Scedosporium</topic><topic>soil</topic><topic>Soil contamination</topic><topic>Soil microorganisms</topic><topic>Soil pollution</topic><topic>Soil Science & Conservation</topic><topic>Soils</topic><topic>Stenotrophomonas</topic><topic>Stenotrophomonas maltophilia</topic><topic>Streptomyces</topic><topic>Trichoderma</topic><topic>Trichoderma asperellum</topic><topic>Water Quality/Water Pollution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zafra, German</creatorcontrib><creatorcontrib>Absalón, Ángel E</creatorcontrib><creatorcontrib>Cuevas, Ma. Del Carmen</creatorcontrib><creatorcontrib>Cortés-Espinosa, Diana V</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Aqualine</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Pollution Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Water Resources Abstracts</collection><collection>Access via ABI/INFORM (ProQuest)</collection><collection>ABI/INFORM Global (PDF only)</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ABI/INFORM Global (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ABI/INFORM Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Business Premium Collection</collection><collection>Natural Science Collection</collection><collection>Earth, Atmospheric & Aquatic Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Business Premium Collection (Alumni)</collection><collection>Health Research Premium Collection</collection><collection>ABI/INFORM Global (Corporate)</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 2: Ocean Technology, Policy & Non-Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Business Collection (Alumni Edition)</collection><collection>ProQuest Business Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ABI/INFORM Professional Advanced</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>ABI/INFORM Global</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Earth, Atmospheric & Aquatic Science Database</collection><collection>ProQuest One Business</collection><collection>ProQuest One Business (Alumni)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Environmental Science Collection</collection><collection>ProQuest Central Basic</collection><collection>Biotechnology Research Abstracts</collection><collection>Environment Abstracts</collection><collection>Sustainability Science Abstracts</collection><collection>Environment Abstracts</collection><jtitle>Water, air, and soil pollution</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zafra, German</au><au>Absalón, Ángel E</au><au>Cuevas, Ma. Del Carmen</au><au>Cortés-Espinosa, Diana V</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and Selection of a Highly Tolerant Microbial Consortium with Potential for PAH Biodegradation from Heavy Crude Oil-Contaminated Soils</atitle><jtitle>Water, air, and soil pollution</jtitle><stitle>Water Air Soil Pollut</stitle><date>2014-02-01</date><risdate>2014</risdate><volume>225</volume><issue>2</issue><spage>1</spage><epage>18</epage><pages>1-18</pages><artnum>1826</artnum><issn>0049-6979</issn><eissn>1573-2932</eissn><abstract>A degrading microbial consortium highly tolerant to three-, four- and five-ring polycyclic aromatic hydrocarbons (PAHs) was selected from 50 fungal and bacterial isolates obtained from crude oil-contaminated soils. Morphological and molecular studies indicated that isolated fungi belonged to genera Aspergillus, Penicillium, Fusarium, Trichoderma, Scedosporium, and Acremonium and bacteria to Pseudomonas, Klebsiella, Bacillus, Enterobacter, Streptomyces, Stenotrophomonas, Kocuria, and Delftia genera. Individual fungal and bacterial isolates were evaluated for their potential to tolerate high concentrations of different molecular weight PAHs, as phenantrene (Phe), pyrene (Pyr), and benzo[a]pyrene (BaP) by surface plate assays, showing significant differences in extension rates for fungi and inhibition ratios for bacteria when both were exposed to 0–6,000 mg of PAHs per liter. Trichoderma asperellum H15, Aspergillus nomius H7, Aspergillus flavus H6, Pseudomonas aeruginosa B7, Klebsiella sp. B10, and Stenotrophomonas maltophilia B14 grew using PAHs as sole carbon source and presented a remarkably high tolerance to PAHs, up to 6,000 mg l⁻¹. The consortium composed of 12 fungal and bacterial PAH-tolerant isolates for the bioremediation of a PAH-contaminated soiled to a removal of 87.76 % Phe, 48.18 % Pyr, and 56.55 % BaP after 14 days. The degrading microbial consortium presented high potential for bioremediation and may be useful for the treatment of sites polluted with PAHs due to their elevated tolerance to high molecular weight (HMW) PAHs and their capacity to utilize them as energy source. This is the first study which evaluated the microbial tolerance to extreme concentrations of PAHs, resulting in a degrading consortium and highly tolerant consortium compared with those reported in other studies, where the concentrations tested are low.</abstract><cop>Cham</cop><pub>Springer-Verlag</pub><doi>10.1007/s11270-013-1826-4</doi><tpages>18</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0049-6979 |
| ispartof | Water, air, and soil pollution, 2014-02, Vol.225 (2), p.1-18, Article 1826 |
| issn | 0049-6979 1573-2932 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1505340464 |
| source | SpringerNature Journals |
| subjects | Acremonium Aspergillus Aspergillus flavus Aspergillus nomius Atmospheric Protection/Air Quality Control/Air Pollution Bacillus Bacteria Benzo(a)pyrene Benzopyrene Biodegradation Bioremediation Carbon sources Climate Change/Climate Change Impacts Consortia Crude oil Delftia Earth and Environmental Science Energy sources Enterobacter Environment Environmental impact Environmental monitoring Environmental studies Enzymes Fungi Fusarium Hydrogeology Klebsiella Kocuria Metabolism Microbial contamination Microorganisms Molecular weight Oil Oil pollution Oxidation Penicillium Pollutants Polycyclic aromatic hydrocarbons Pseudomonas aeruginosa Pyrene Scedosporium soil Soil contamination Soil microorganisms Soil pollution Soil Science & Conservation Soils Stenotrophomonas Stenotrophomonas maltophilia Streptomyces Trichoderma Trichoderma asperellum Water Quality/Water Pollution |
| title | Isolation and Selection of a Highly Tolerant Microbial Consortium with Potential for PAH Biodegradation from Heavy Crude Oil-Contaminated Soils |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-02T18%3A41%3A25IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Isolation%20and%20Selection%20of%20a%20Highly%20Tolerant%20Microbial%20Consortium%20with%20Potential%20for%20PAH%20Biodegradation%20from%20Heavy%20Crude%20Oil-Contaminated%20Soils&rft.jtitle=Water,%20air,%20and%20soil%20pollution&rft.au=Zafra,%20German&rft.date=2014-02-01&rft.volume=225&rft.issue=2&rft.spage=1&rft.epage=18&rft.pages=1-18&rft.artnum=1826&rft.issn=0049-6979&rft.eissn=1573-2932&rft_id=info:doi/10.1007/s11270-013-1826-4&rft_dat=%3Cgale_proqu%3EA369129764%3C/gale_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1499478330&rft_id=info:pmid/&rft_galeid=A369129764&rfr_iscdi=true |