In vitro effect of cortisol and urotensin I on arginine vasotocin and isotocin secretion from pituitary cells of gilthead sea bream Sparus aurata
This study aimed at determining whether in vitro secretion of two neuropeptides, arginine vasotocin (AVT) and isotocin (IT), from pituitary cells of gilthead sea bream Sparus aurata was affected by cortisol and urotensin (UI). Pituitary cells were exposed to 1·4 × 10−8, 1·4 × 10−7 and 0·4 × 10−6 M c...
Gespeichert in:
| Veröffentlicht in: | Journal of fish biology 2014-02, Vol.84 (2), p.448-458 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 458 |
|---|---|
| container_issue | 2 |
| container_start_page | 448 |
| container_title | Journal of fish biology |
| container_volume | 84 |
| creator | Kalamarz-Kubiak, H. Meiri-Ashkenazi, I. Kleszczyńska, A. Rosenfeld, H. |
| description | This study aimed at determining whether in vitro secretion of two neuropeptides, arginine vasotocin (AVT) and isotocin (IT), from pituitary cells of gilthead sea bream Sparus aurata was affected by cortisol and urotensin (UI). Pituitary cells were exposed to 1·4 × 10−8, 1·4 × 10−7 and 0·4 × 10−6 M cortisol and 10−12, 10−10 and 10−8 M UI for 6, 24 and 48 h, respectively. AVT and IT contents were determined in the culture media by high‐performance liquid chromatography (HPLC). An increase in AVT secretion and a decrease in IT secretion were observed at all cortisol doses. UI increased AVT secretion after 6 h of incubation at all doses. After 24 h, however, only the highest dose of UI still displayed an effect. IT secretion was not influenced by UI. It was thus demonstrated that cortisol does influence AVT and IT secretion from S. aurata pituitary cells, while UI regulates AVT secretion, as a component of hypothalamic–pituitary–interrenal (HPI) axis in this species. |
| doi_str_mv | 10.1111/jfb.12297 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1505338819</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1505338819</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4247-372c15d159634d6a74459ea95b635a3c01af4063b96f0417b51e5ab53a67cafd3</originalsourceid><addsrcrecordid>eNqF0U9vFCEYBnBiNHatHvwChsRLPUwLw7_l6DZuXdOosZoaL-Qdhqmss8MKTLUfw28s43Z7MDFyIZAfT4AHoaeUHNMyTtZdc0zrWqt7aEaJFtVccn0fzQip66qA-gA9SmlNCNFMs4fooOacCK7oDP1aDfja5xiw6zpnMw4dtiFmn0KPYWjxGEN2Q_IDXuEwYIhXfvCDw9eQQg627E_K7xfJ2eiyL7KLYYO3Po8-Q7zB1vV9mtKvfJ-_OmgLBdxEBxt8sYU4JgxjhAyP0YMO-uSe3M6H6NPy1cfT19X5u7PV6cvzyvKaq4qp2lLRUqEl460ExbnQDrRoJBPALKHQcSJZo2VHOFWNoE5AIxhIZaFr2SE62uVuY_g-upTNxqfpljC4MCZDBRGMzedU_59yrSmdKznR53_RdRjjUB4yKaZUrSQv6sVO2RhSiq4z2-g35ZsMJWaq1JRKzZ9Ki312mzg2G9feyX2HBZzswA_fu5t_J5k3y8U-stqd8Cm7n3cnIH4zUjElzOXbM_P5_fLD4uLLpVmw30qGupI</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1493772764</pqid></control><display><type>article</type><title>In vitro effect of cortisol and urotensin I on arginine vasotocin and isotocin secretion from pituitary cells of gilthead sea bream Sparus aurata</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Kalamarz-Kubiak, H. ; Meiri-Ashkenazi, I. ; Kleszczyńska, A. ; Rosenfeld, H.</creator><creatorcontrib>Kalamarz-Kubiak, H. ; Meiri-Ashkenazi, I. ; Kleszczyńska, A. ; Rosenfeld, H.</creatorcontrib><description>This study aimed at determining whether in vitro secretion of two neuropeptides, arginine vasotocin (AVT) and isotocin (IT), from pituitary cells of gilthead sea bream Sparus aurata was affected by cortisol and urotensin (UI). Pituitary cells were exposed to 1·4 × 10−8, 1·4 × 10−7 and 0·4 × 10−6 M cortisol and 10−12, 10−10 and 10−8 M UI for 6, 24 and 48 h, respectively. AVT and IT contents were determined in the culture media by high‐performance liquid chromatography (HPLC). An increase in AVT secretion and a decrease in IT secretion were observed at all cortisol doses. UI increased AVT secretion after 6 h of incubation at all doses. After 24 h, however, only the highest dose of UI still displayed an effect. IT secretion was not influenced by UI. It was thus demonstrated that cortisol does influence AVT and IT secretion from S. aurata pituitary cells, while UI regulates AVT secretion, as a component of hypothalamic–pituitary–interrenal (HPI) axis in this species.</description><identifier>ISSN: 0022-1112</identifier><identifier>EISSN: 1095-8649</identifier><identifier>DOI: 10.1111/jfb.12297</identifier><identifier>PMID: 24405471</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Animals ; Cells, Cultured ; Chromatography, High Pressure Liquid ; Female ; fish nonapeptides ; Hydrocortisone - pharmacology ; Male ; Marine ; Oxytocin - analogs & derivatives ; Oxytocin - secretion ; Pituitary Gland - cytology ; Pituitary Gland - secretion ; primary hypophysis cell cultures ; Sea Bream ; Sparus aurata ; stress hormones ; Urotensins - pharmacology ; Vasotocin - secretion</subject><ispartof>Journal of fish biology, 2014-02, Vol.84 (2), p.448-458</ispartof><rights>2014 The Fisheries Society of the British Isles</rights><rights>2014 The Fisheries Society of the British Isles.</rights><rights>Journal of Fish Biology © 2014 The Fisheries Society of the British Isles</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4247-372c15d159634d6a74459ea95b635a3c01af4063b96f0417b51e5ab53a67cafd3</citedby><cites>FETCH-LOGICAL-c4247-372c15d159634d6a74459ea95b635a3c01af4063b96f0417b51e5ab53a67cafd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjfb.12297$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjfb.12297$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24405471$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kalamarz-Kubiak, H.</creatorcontrib><creatorcontrib>Meiri-Ashkenazi, I.</creatorcontrib><creatorcontrib>Kleszczyńska, A.</creatorcontrib><creatorcontrib>Rosenfeld, H.</creatorcontrib><title>In vitro effect of cortisol and urotensin I on arginine vasotocin and isotocin secretion from pituitary cells of gilthead sea bream Sparus aurata</title><title>Journal of fish biology</title><addtitle>J Fish Biol</addtitle><description>This study aimed at determining whether in vitro secretion of two neuropeptides, arginine vasotocin (AVT) and isotocin (IT), from pituitary cells of gilthead sea bream Sparus aurata was affected by cortisol and urotensin (UI). Pituitary cells were exposed to 1·4 × 10−8, 1·4 × 10−7 and 0·4 × 10−6 M cortisol and 10−12, 10−10 and 10−8 M UI for 6, 24 and 48 h, respectively. AVT and IT contents were determined in the culture media by high‐performance liquid chromatography (HPLC). An increase in AVT secretion and a decrease in IT secretion were observed at all cortisol doses. UI increased AVT secretion after 6 h of incubation at all doses. After 24 h, however, only the highest dose of UI still displayed an effect. IT secretion was not influenced by UI. It was thus demonstrated that cortisol does influence AVT and IT secretion from S. aurata pituitary cells, while UI regulates AVT secretion, as a component of hypothalamic–pituitary–interrenal (HPI) axis in this species.</description><subject>Animals</subject><subject>Cells, Cultured</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Female</subject><subject>fish nonapeptides</subject><subject>Hydrocortisone - pharmacology</subject><subject>Male</subject><subject>Marine</subject><subject>Oxytocin - analogs & derivatives</subject><subject>Oxytocin - secretion</subject><subject>Pituitary Gland - cytology</subject><subject>Pituitary Gland - secretion</subject><subject>primary hypophysis cell cultures</subject><subject>Sea Bream</subject><subject>Sparus aurata</subject><subject>stress hormones</subject><subject>Urotensins - pharmacology</subject><subject>Vasotocin - secretion</subject><issn>0022-1112</issn><issn>1095-8649</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0U9vFCEYBnBiNHatHvwChsRLPUwLw7_l6DZuXdOosZoaL-Qdhqmss8MKTLUfw28s43Z7MDFyIZAfT4AHoaeUHNMyTtZdc0zrWqt7aEaJFtVccn0fzQip66qA-gA9SmlNCNFMs4fooOacCK7oDP1aDfja5xiw6zpnMw4dtiFmn0KPYWjxGEN2Q_IDXuEwYIhXfvCDw9eQQg627E_K7xfJ2eiyL7KLYYO3Po8-Q7zB1vV9mtKvfJ-_OmgLBdxEBxt8sYU4JgxjhAyP0YMO-uSe3M6H6NPy1cfT19X5u7PV6cvzyvKaq4qp2lLRUqEl460ExbnQDrRoJBPALKHQcSJZo2VHOFWNoE5AIxhIZaFr2SE62uVuY_g-upTNxqfpljC4MCZDBRGMzedU_59yrSmdKznR53_RdRjjUB4yKaZUrSQv6sVO2RhSiq4z2-g35ZsMJWaq1JRKzZ9Ki312mzg2G9feyX2HBZzswA_fu5t_J5k3y8U-stqd8Cm7n3cnIH4zUjElzOXbM_P5_fLD4uLLpVmw30qGupI</recordid><startdate>201402</startdate><enddate>201402</enddate><creator>Kalamarz-Kubiak, H.</creator><creator>Meiri-Ashkenazi, I.</creator><creator>Kleszczyńska, A.</creator><creator>Rosenfeld, H.</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7SN</scope><scope>7TN</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>L.G</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>H98</scope><scope>H99</scope><scope>L.F</scope></search><sort><creationdate>201402</creationdate><title>In vitro effect of cortisol and urotensin I on arginine vasotocin and isotocin secretion from pituitary cells of gilthead sea bream Sparus aurata</title><author>Kalamarz-Kubiak, H. ; Meiri-Ashkenazi, I. ; Kleszczyńska, A. ; Rosenfeld, H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4247-372c15d159634d6a74459ea95b635a3c01af4063b96f0417b51e5ab53a67cafd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Cells, Cultured</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Female</topic><topic>fish nonapeptides</topic><topic>Hydrocortisone - pharmacology</topic><topic>Male</topic><topic>Marine</topic><topic>Oxytocin - analogs & derivatives</topic><topic>Oxytocin - secretion</topic><topic>Pituitary Gland - cytology</topic><topic>Pituitary Gland - secretion</topic><topic>primary hypophysis cell cultures</topic><topic>Sea Bream</topic><topic>Sparus aurata</topic><topic>stress hormones</topic><topic>Urotensins - pharmacology</topic><topic>Vasotocin - secretion</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kalamarz-Kubiak, H.</creatorcontrib><creatorcontrib>Meiri-Ashkenazi, I.</creatorcontrib><creatorcontrib>Kleszczyńska, A.</creatorcontrib><creatorcontrib>Rosenfeld, H.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Ecology Abstracts</collection><collection>Oceanic Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><jtitle>Journal of fish biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kalamarz-Kubiak, H.</au><au>Meiri-Ashkenazi, I.</au><au>Kleszczyńska, A.</au><au>Rosenfeld, H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro effect of cortisol and urotensin I on arginine vasotocin and isotocin secretion from pituitary cells of gilthead sea bream Sparus aurata</atitle><jtitle>Journal of fish biology</jtitle><addtitle>J Fish Biol</addtitle><date>2014-02</date><risdate>2014</risdate><volume>84</volume><issue>2</issue><spage>448</spage><epage>458</epage><pages>448-458</pages><issn>0022-1112</issn><eissn>1095-8649</eissn><abstract>This study aimed at determining whether in vitro secretion of two neuropeptides, arginine vasotocin (AVT) and isotocin (IT), from pituitary cells of gilthead sea bream Sparus aurata was affected by cortisol and urotensin (UI). Pituitary cells were exposed to 1·4 × 10−8, 1·4 × 10−7 and 0·4 × 10−6 M cortisol and 10−12, 10−10 and 10−8 M UI for 6, 24 and 48 h, respectively. AVT and IT contents were determined in the culture media by high‐performance liquid chromatography (HPLC). An increase in AVT secretion and a decrease in IT secretion were observed at all cortisol doses. UI increased AVT secretion after 6 h of incubation at all doses. After 24 h, however, only the highest dose of UI still displayed an effect. IT secretion was not influenced by UI. It was thus demonstrated that cortisol does influence AVT and IT secretion from S. aurata pituitary cells, while UI regulates AVT secretion, as a component of hypothalamic–pituitary–interrenal (HPI) axis in this species.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>24405471</pmid><doi>10.1111/jfb.12297</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-1112 |
| ispartof | Journal of fish biology, 2014-02, Vol.84 (2), p.448-458 |
| issn | 0022-1112 1095-8649 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1505338819 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals Cells, Cultured Chromatography, High Pressure Liquid Female fish nonapeptides Hydrocortisone - pharmacology Male Marine Oxytocin - analogs & derivatives Oxytocin - secretion Pituitary Gland - cytology Pituitary Gland - secretion primary hypophysis cell cultures Sea Bream Sparus aurata stress hormones Urotensins - pharmacology Vasotocin - secretion |
| title | In vitro effect of cortisol and urotensin I on arginine vasotocin and isotocin secretion from pituitary cells of gilthead sea bream Sparus aurata |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-27T11%3A52%3A52IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=In%20vitro%20effect%20of%20cortisol%20and%20urotensin%20I%20on%20arginine%20vasotocin%20and%20isotocin%20secretion%20from%20pituitary%20cells%20of%20gilthead%20sea%20bream%20Sparus%20aurata&rft.jtitle=Journal%20of%20fish%20biology&rft.au=Kalamarz-Kubiak,%20H.&rft.date=2014-02&rft.volume=84&rft.issue=2&rft.spage=448&rft.epage=458&rft.pages=448-458&rft.issn=0022-1112&rft.eissn=1095-8649&rft_id=info:doi/10.1111/jfb.12297&rft_dat=%3Cproquest_cross%3E1505338819%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1493772764&rft_id=info:pmid/24405471&rfr_iscdi=true |