Differences in bacterial saliva profile between periodontitis patients and a control cohort
Aim Periodontitis is a multifactorial disease in which subgingival bacteria play an important role in the pathogenesis of the disease. The objective of this study was to determine if periodontitis is associated with a characteristic salivary bacterial profile. This was accomplished by comparing the...
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| Veröffentlicht in: | Journal of clinical periodontology 2014-02, Vol.41 (2), p.104-112 |
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| container_title | Journal of clinical periodontology |
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| creator | Belstrøm, Daniel Fiehn, Nils-Erik Nielsen, Claus H. Kirkby, Nikolai Twetman, Svante Klepac-Ceraj, Vanja Paster, Bruce J. Holmstrup, Palle |
| description | Aim
Periodontitis is a multifactorial disease in which subgingival bacteria play an important role in the pathogenesis of the disease. The objective of this study was to determine if periodontitis is associated with a characteristic salivary bacterial profile. This was accomplished by comparing the bacterial profile of saliva from subjects with chronic periodontitis with that of saliva from a control cohort.
Materials and methods
Stimulated saliva samples from 139 chronic periodontitis patients and 447 samples from a control cohort were analysed using the Human Oral Microbe Identification Microarray (HOMIM). Frequency and levels (mean HOMIM‐value) of around 300 bacterial taxa/clusters in samples were used as parameters for investigation. Differences at taxon/cluster values between groups were analysed using Mann–Whitney U‐test with Benjamini–Hochberg correction for multiple comparisons. Principal component analysis was used to visualize bacterial community profiles obtained by the HOMIM.
Results
Eight bacterial taxa, including putative periodontal pathogens as Parvimonas micra and Filifactor alocis, and four bacterial clusters were identified statistically more frequently and at higher levels in samples from periodontitis patients than in samples from the control cohort. These differences were independent of the individuals' smoking status.
Conclusions
Periodontitis is associated with a characteristic bacterial profile of saliva different from that of a control cohort. |
| doi_str_mv | 10.1111/jcpe.12190 |
| format | Article |
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Periodontitis is a multifactorial disease in which subgingival bacteria play an important role in the pathogenesis of the disease. The objective of this study was to determine if periodontitis is associated with a characteristic salivary bacterial profile. This was accomplished by comparing the bacterial profile of saliva from subjects with chronic periodontitis with that of saliva from a control cohort.
Materials and methods
Stimulated saliva samples from 139 chronic periodontitis patients and 447 samples from a control cohort were analysed using the Human Oral Microbe Identification Microarray (HOMIM). Frequency and levels (mean HOMIM‐value) of around 300 bacterial taxa/clusters in samples were used as parameters for investigation. Differences at taxon/cluster values between groups were analysed using Mann–Whitney U‐test with Benjamini–Hochberg correction for multiple comparisons. Principal component analysis was used to visualize bacterial community profiles obtained by the HOMIM.
Results
Eight bacterial taxa, including putative periodontal pathogens as Parvimonas micra and Filifactor alocis, and four bacterial clusters were identified statistically more frequently and at higher levels in samples from periodontitis patients than in samples from the control cohort. These differences were independent of the individuals' smoking status.
Conclusions
Periodontitis is associated with a characteristic bacterial profile of saliva different from that of a control cohort.</description><identifier>ISSN: 0303-6979</identifier><identifier>EISSN: 1600-051X</identifier><identifier>DOI: 10.1111/jcpe.12190</identifier><identifier>PMID: 24303924</identifier><language>eng</language><publisher>United States: Blackwell Publishing Ltd</publisher><subject>16S rRNA ; Actinobacteria - classification ; Bacteria ; Bacteria - classification ; Bacterial Load ; Bacteroidetes - classification ; biomarker ; Body fluids ; Chronic Periodontitis - microbiology ; Cohort Studies ; Dental Caries - complications ; Dentistry ; Female ; Gram-Negative Bacteria - classification ; Gum disease ; HOMIM ; Humans ; Male ; Microarray Analysis ; Middle Aged ; Periodontal Attachment Loss - microbiology ; Periodontal Index ; Periodontal Pocket - microbiology ; periodontitis ; Population Surveillance ; Principal Component Analysis ; Proteobacteria - classification ; saliva ; Saliva - microbiology ; Smoking ; Streptococcus - classification</subject><ispartof>Journal of clinical periodontology, 2014-02, Vol.41 (2), p.104-112</ispartof><rights>2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd</rights><rights>2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.</rights><rights>Copyright © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4940-1fe6f1944f5d8a173be5275230b040645b7613702725c2c6b03655217b7665e23</citedby><cites>FETCH-LOGICAL-c4940-1fe6f1944f5d8a173be5275230b040645b7613702725c2c6b03655217b7665e23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjcpe.12190$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjcpe.12190$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24303924$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Belstrøm, Daniel</creatorcontrib><creatorcontrib>Fiehn, Nils-Erik</creatorcontrib><creatorcontrib>Nielsen, Claus H.</creatorcontrib><creatorcontrib>Kirkby, Nikolai</creatorcontrib><creatorcontrib>Twetman, Svante</creatorcontrib><creatorcontrib>Klepac-Ceraj, Vanja</creatorcontrib><creatorcontrib>Paster, Bruce J.</creatorcontrib><creatorcontrib>Holmstrup, Palle</creatorcontrib><title>Differences in bacterial saliva profile between periodontitis patients and a control cohort</title><title>Journal of clinical periodontology</title><addtitle>J Clin Periodontol</addtitle><description>Aim
Periodontitis is a multifactorial disease in which subgingival bacteria play an important role in the pathogenesis of the disease. The objective of this study was to determine if periodontitis is associated with a characteristic salivary bacterial profile. This was accomplished by comparing the bacterial profile of saliva from subjects with chronic periodontitis with that of saliva from a control cohort.
Materials and methods
Stimulated saliva samples from 139 chronic periodontitis patients and 447 samples from a control cohort were analysed using the Human Oral Microbe Identification Microarray (HOMIM). Frequency and levels (mean HOMIM‐value) of around 300 bacterial taxa/clusters in samples were used as parameters for investigation. Differences at taxon/cluster values between groups were analysed using Mann–Whitney U‐test with Benjamini–Hochberg correction for multiple comparisons. Principal component analysis was used to visualize bacterial community profiles obtained by the HOMIM.
Results
Eight bacterial taxa, including putative periodontal pathogens as Parvimonas micra and Filifactor alocis, and four bacterial clusters were identified statistically more frequently and at higher levels in samples from periodontitis patients than in samples from the control cohort. These differences were independent of the individuals' smoking status.
Conclusions
Periodontitis is associated with a characteristic bacterial profile of saliva different from that of a control cohort.</description><subject>16S rRNA</subject><subject>Actinobacteria - classification</subject><subject>Bacteria</subject><subject>Bacteria - classification</subject><subject>Bacterial Load</subject><subject>Bacteroidetes - classification</subject><subject>biomarker</subject><subject>Body fluids</subject><subject>Chronic Periodontitis - microbiology</subject><subject>Cohort Studies</subject><subject>Dental Caries - complications</subject><subject>Dentistry</subject><subject>Female</subject><subject>Gram-Negative Bacteria - classification</subject><subject>Gum disease</subject><subject>HOMIM</subject><subject>Humans</subject><subject>Male</subject><subject>Microarray Analysis</subject><subject>Middle Aged</subject><subject>Periodontal Attachment Loss - microbiology</subject><subject>Periodontal Index</subject><subject>Periodontal Pocket - microbiology</subject><subject>periodontitis</subject><subject>Population Surveillance</subject><subject>Principal Component Analysis</subject><subject>Proteobacteria - classification</subject><subject>saliva</subject><subject>Saliva - microbiology</subject><subject>Smoking</subject><subject>Streptococcus - classification</subject><issn>0303-6979</issn><issn>1600-051X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1vFDEMhiMEokvhwg9AkbggpCl2Ppkj2pbysQIkQCA4RJmsR2SZnRmSLKX_npRte-AAvliyH7-y_TJ2H-EIazzZhJmOUGALN9gCDUADGj_fZAuQIBvT2vaA3cl5A4BWSnmbHQhVO61QC_b1OPY9JRoDZR5H3vlQKEU_8OyH-NPzOU19HIh3VM6IRj7X7rSexhJLzHz2JdJYMvfjmnseaj1NQ83fplTuslu9HzLdu8yH7OPzkw_LF83q7enL5bNVE1SroMGeTI-tUr1eP_V1xY60sFpI6ECBUbqzBqUFYYUOIpgOpNFaoK11o0nIQ_Zor1t3_bGjXNw25kDD4EeadtmhBi2lQoT_o6oFa5U1bUUf_oVupl0a6yGVslYqbQRW6vGeCmnKOVHv5hS3Pp07BHfhjrtwx_1xp8IPLiV33ZbW1-iVHRXAPXBWX37-Dyn3avnu5Eq02c_EXOjX9YxP352x0mr36c2pWxqQq9fvjfsifwPfHKak</recordid><startdate>201402</startdate><enddate>201402</enddate><creator>Belstrøm, Daniel</creator><creator>Fiehn, Nils-Erik</creator><creator>Nielsen, Claus H.</creator><creator>Kirkby, Nikolai</creator><creator>Twetman, Svante</creator><creator>Klepac-Ceraj, Vanja</creator><creator>Paster, Bruce J.</creator><creator>Holmstrup, Palle</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>K9.</scope><scope>7X8</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>201402</creationdate><title>Differences in bacterial saliva profile between periodontitis patients and a control cohort</title><author>Belstrøm, Daniel ; Fiehn, Nils-Erik ; Nielsen, Claus H. ; Kirkby, Nikolai ; Twetman, Svante ; Klepac-Ceraj, Vanja ; Paster, Bruce J. ; Holmstrup, Palle</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4940-1fe6f1944f5d8a173be5275230b040645b7613702725c2c6b03655217b7665e23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>16S rRNA</topic><topic>Actinobacteria - classification</topic><topic>Bacteria</topic><topic>Bacteria - classification</topic><topic>Bacterial Load</topic><topic>Bacteroidetes - classification</topic><topic>biomarker</topic><topic>Body fluids</topic><topic>Chronic Periodontitis - microbiology</topic><topic>Cohort Studies</topic><topic>Dental Caries - complications</topic><topic>Dentistry</topic><topic>Female</topic><topic>Gram-Negative Bacteria - classification</topic><topic>Gum disease</topic><topic>HOMIM</topic><topic>Humans</topic><topic>Male</topic><topic>Microarray Analysis</topic><topic>Middle Aged</topic><topic>Periodontal Attachment Loss - microbiology</topic><topic>Periodontal Index</topic><topic>Periodontal Pocket - microbiology</topic><topic>periodontitis</topic><topic>Population Surveillance</topic><topic>Principal Component Analysis</topic><topic>Proteobacteria - classification</topic><topic>saliva</topic><topic>Saliva - microbiology</topic><topic>Smoking</topic><topic>Streptococcus - classification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Belstrøm, Daniel</creatorcontrib><creatorcontrib>Fiehn, Nils-Erik</creatorcontrib><creatorcontrib>Nielsen, Claus H.</creatorcontrib><creatorcontrib>Kirkby, Nikolai</creatorcontrib><creatorcontrib>Twetman, Svante</creatorcontrib><creatorcontrib>Klepac-Ceraj, Vanja</creatorcontrib><creatorcontrib>Paster, Bruce J.</creatorcontrib><creatorcontrib>Holmstrup, Palle</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of clinical periodontology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Belstrøm, Daniel</au><au>Fiehn, Nils-Erik</au><au>Nielsen, Claus H.</au><au>Kirkby, Nikolai</au><au>Twetman, Svante</au><au>Klepac-Ceraj, Vanja</au><au>Paster, Bruce J.</au><au>Holmstrup, Palle</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differences in bacterial saliva profile between periodontitis patients and a control cohort</atitle><jtitle>Journal of clinical periodontology</jtitle><addtitle>J Clin Periodontol</addtitle><date>2014-02</date><risdate>2014</risdate><volume>41</volume><issue>2</issue><spage>104</spage><epage>112</epage><pages>104-112</pages><issn>0303-6979</issn><eissn>1600-051X</eissn><abstract>Aim
Periodontitis is a multifactorial disease in which subgingival bacteria play an important role in the pathogenesis of the disease. The objective of this study was to determine if periodontitis is associated with a characteristic salivary bacterial profile. This was accomplished by comparing the bacterial profile of saliva from subjects with chronic periodontitis with that of saliva from a control cohort.
Materials and methods
Stimulated saliva samples from 139 chronic periodontitis patients and 447 samples from a control cohort were analysed using the Human Oral Microbe Identification Microarray (HOMIM). Frequency and levels (mean HOMIM‐value) of around 300 bacterial taxa/clusters in samples were used as parameters for investigation. Differences at taxon/cluster values between groups were analysed using Mann–Whitney U‐test with Benjamini–Hochberg correction for multiple comparisons. Principal component analysis was used to visualize bacterial community profiles obtained by the HOMIM.
Results
Eight bacterial taxa, including putative periodontal pathogens as Parvimonas micra and Filifactor alocis, and four bacterial clusters were identified statistically more frequently and at higher levels in samples from periodontitis patients than in samples from the control cohort. These differences were independent of the individuals' smoking status.
Conclusions
Periodontitis is associated with a characteristic bacterial profile of saliva different from that of a control cohort.</abstract><cop>United States</cop><pub>Blackwell Publishing Ltd</pub><pmid>24303924</pmid><doi>10.1111/jcpe.12190</doi><tpages>9</tpages></addata></record> |
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| identifier | ISSN: 0303-6979 |
| ispartof | Journal of clinical periodontology, 2014-02, Vol.41 (2), p.104-112 |
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| subjects | 16S rRNA Actinobacteria - classification Bacteria Bacteria - classification Bacterial Load Bacteroidetes - classification biomarker Body fluids Chronic Periodontitis - microbiology Cohort Studies Dental Caries - complications Dentistry Female Gram-Negative Bacteria - classification Gum disease HOMIM Humans Male Microarray Analysis Middle Aged Periodontal Attachment Loss - microbiology Periodontal Index Periodontal Pocket - microbiology periodontitis Population Surveillance Principal Component Analysis Proteobacteria - classification saliva Saliva - microbiology Smoking Streptococcus - classification |
| title | Differences in bacterial saliva profile between periodontitis patients and a control cohort |
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