Verification of mitotic gynogenesis in ornamental (koi) carp (Cyprinus carpio L.) using microsatellite DNA markers
The Japanese ornamental (koi) carp is a popular decorative fish all over the world. In koi, clones have not yet been obtained, although production of fish with identical colour patterns could be of commercial interest. Mitotic gynogenetic progenies are essential for subsequent production of clones i...
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| Veröffentlicht in: | Aquaculture research 2014-02, Vol.45 (3), p.410-416 |
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| creator | Alsaqufi, Ahmed S Gomelsky, Boris Schneider, Kyle J Pomper, Kirk W |
| description | The Japanese ornamental (koi) carp is a popular decorative fish all over the world. In koi, clones have not yet been obtained, although production of fish with identical colour patterns could be of commercial interest. Mitotic gynogenetic progenies are essential for subsequent production of clones in fish. However, resulting late‐shocked progenies may be contaminated with meiotic gynogens from spontaneous suppression of the second meiotic division in eggs. In this study, microsatellite DNA markers were used to confirm mitotic gynogenetic origin of obtained late‐shocked progenies. Recombination rate (y) and mapping distance relative to centromere (M‐C) of 10 microsatellite loci were determined based on percentage of heterozygotes in meiotic gynogenetic progenies. The range of y varied from 0.01 to 0.96 and the M‐C map ranged from 0.5 to 48 cM. The mean value of y over the 10 loci was 0.481. Six loci, which had y 0.47 and higher, were used as markers in two late‐shocked gynogenetic progenies. Complete homozygosity was revealed at all six microsatellite loci indicating mitotic gynogenetic origin of analysed progenies. |
| doi_str_mv | 10.1111/j.1365-2109.2012.03242.x |
| format | Article |
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In koi, clones have not yet been obtained, although production of fish with identical colour patterns could be of commercial interest. Mitotic gynogenetic progenies are essential for subsequent production of clones in fish. However, resulting late‐shocked progenies may be contaminated with meiotic gynogens from spontaneous suppression of the second meiotic division in eggs. In this study, microsatellite DNA markers were used to confirm mitotic gynogenetic origin of obtained late‐shocked progenies. Recombination rate (y) and mapping distance relative to centromere (M‐C) of 10 microsatellite loci were determined based on percentage of heterozygotes in meiotic gynogenetic progenies. The range of y varied from 0.01 to 0.96 and the M‐C map ranged from 0.5 to 48 cM. The mean value of y over the 10 loci was 0.481. Six loci, which had y 0.47 and higher, were used as markers in two late‐shocked gynogenetic progenies. Complete homozygosity was revealed at all six microsatellite loci indicating mitotic gynogenetic origin of analysed progenies.</description><identifier>ISSN: 1355-557X</identifier><identifier>EISSN: 1365-2109</identifier><identifier>DOI: 10.1111/j.1365-2109.2012.03242.x</identifier><language>eng</language><publisher>Oxford: Blackwell Science</publisher><subject>clones ; color ; common carp ; Cyprinus carpio ; Deoxyribonucleic acid ; DNA ; DNA markers ; eggs ; Fish ; fish production ; Freshwater ; Gene loci ; Genetic markers ; gynogenesis ; homozygosity ; koi ; loci ; meiosis ; microsatellite repeats ; microsatellites</subject><ispartof>Aquaculture research, 2014-02, Vol.45 (3), p.410-416</ispartof><rights>2012 John Wiley & Sons Ltd</rights><rights>Copyright © 2014 John Wiley & Sons Ltd</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5522-912dedfd305c28e79f5b981e5d3e485199279eceded5cb83c1e2cac7659b214b3</citedby><cites>FETCH-LOGICAL-c5522-912dedfd305c28e79f5b981e5d3e485199279eceded5cb83c1e2cac7659b214b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-2109.2012.03242.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-2109.2012.03242.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27923,27924,45573,45574</link.rule.ids></links><search><creatorcontrib>Alsaqufi, Ahmed S</creatorcontrib><creatorcontrib>Gomelsky, Boris</creatorcontrib><creatorcontrib>Schneider, Kyle J</creatorcontrib><creatorcontrib>Pomper, Kirk W</creatorcontrib><title>Verification of mitotic gynogenesis in ornamental (koi) carp (Cyprinus carpio L.) using microsatellite DNA markers</title><title>Aquaculture research</title><addtitle>Aquac Res</addtitle><description>The Japanese ornamental (koi) carp is a popular decorative fish all over the world. In koi, clones have not yet been obtained, although production of fish with identical colour patterns could be of commercial interest. Mitotic gynogenetic progenies are essential for subsequent production of clones in fish. However, resulting late‐shocked progenies may be contaminated with meiotic gynogens from spontaneous suppression of the second meiotic division in eggs. In this study, microsatellite DNA markers were used to confirm mitotic gynogenetic origin of obtained late‐shocked progenies. Recombination rate (y) and mapping distance relative to centromere (M‐C) of 10 microsatellite loci were determined based on percentage of heterozygotes in meiotic gynogenetic progenies. The range of y varied from 0.01 to 0.96 and the M‐C map ranged from 0.5 to 48 cM. The mean value of y over the 10 loci was 0.481. Six loci, which had y 0.47 and higher, were used as markers in two late‐shocked gynogenetic progenies. Complete homozygosity was revealed at all six microsatellite loci indicating mitotic gynogenetic origin of analysed progenies.</description><subject>clones</subject><subject>color</subject><subject>common carp</subject><subject>Cyprinus carpio</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA markers</subject><subject>eggs</subject><subject>Fish</subject><subject>fish production</subject><subject>Freshwater</subject><subject>Gene loci</subject><subject>Genetic markers</subject><subject>gynogenesis</subject><subject>homozygosity</subject><subject>koi</subject><subject>loci</subject><subject>meiosis</subject><subject>microsatellite repeats</subject><subject>microsatellites</subject><issn>1355-557X</issn><issn>1365-2109</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNqNkU1v1DAQhiMEEqXwG7DEZXtI6s8kPnBYtqWttCqfpdxGXu9k5W02XuxE7P57nAb1wAlfPNY878jvvFlGGC1YOufbgolS5ZxRXXDKeEEFl7w4PMtOnhrPx1qpXKnq58vsVYxbSpmkgp1k4QcG1zhreuc74huyc73vnSWbY-c32GF0kbjUCZ3ZYdeblswevDsj1oQ9mS2O--C6IT4-nSfL4owM0XWbNMcGH02Pbet6JBe3c7Iz4QFDfJ29aEwb8c3f-zS7-3j5fXGdLz9d3Szmy9wqxXmuGV_julkLqiyvsdKNWumaoVoLlLViWvNKo8UEKbuqhWXIrbFVqfSKM7kSp9lsmrsP_teAsYedizb9x3TohwhMUSW4LilL6Lt_0K0fkuM2UVLLUkgpRKLqiRqdxYANJPPJ1BEYhTEM2MK4cxh3DmMY8BgGHJL0_ST97Vo8_rcO5l8vxyrp80nvYo-HJ31aKJSVqBTc317B5_L-4sOX6woWiX878Y3xYDbBRbj7xsfQ03TJdCn-AAV-qGU</recordid><startdate>201402</startdate><enddate>201402</enddate><creator>Alsaqufi, Ahmed S</creator><creator>Gomelsky, Boris</creator><creator>Schneider, Kyle J</creator><creator>Pomper, Kirk W</creator><general>Blackwell Science</general><general>Blackwell Publishing Ltd</general><general>Hindawi Limited</general><scope>FBQ</scope><scope>BSCLL</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TN</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>H98</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7ST</scope><scope>7TM</scope><scope>SOI</scope></search><sort><creationdate>201402</creationdate><title>Verification of mitotic gynogenesis in ornamental (koi) carp (Cyprinus carpio L.) using microsatellite DNA markers</title><author>Alsaqufi, Ahmed S ; Gomelsky, Boris ; Schneider, Kyle J ; Pomper, Kirk W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5522-912dedfd305c28e79f5b981e5d3e485199279eceded5cb83c1e2cac7659b214b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>clones</topic><topic>color</topic><topic>common carp</topic><topic>Cyprinus carpio</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA markers</topic><topic>eggs</topic><topic>Fish</topic><topic>fish production</topic><topic>Freshwater</topic><topic>Gene loci</topic><topic>Genetic markers</topic><topic>gynogenesis</topic><topic>homozygosity</topic><topic>koi</topic><topic>loci</topic><topic>meiosis</topic><topic>microsatellite repeats</topic><topic>microsatellites</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Alsaqufi, Ahmed S</creatorcontrib><creatorcontrib>Gomelsky, Boris</creatorcontrib><creatorcontrib>Schneider, Kyle J</creatorcontrib><creatorcontrib>Pomper, Kirk W</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>CrossRef</collection><collection>Oceanic Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Environment Abstracts</collection><jtitle>Aquaculture research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Alsaqufi, Ahmed S</au><au>Gomelsky, Boris</au><au>Schneider, Kyle J</au><au>Pomper, Kirk W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Verification of mitotic gynogenesis in ornamental (koi) carp (Cyprinus carpio L.) using microsatellite DNA markers</atitle><jtitle>Aquaculture research</jtitle><addtitle>Aquac Res</addtitle><date>2014-02</date><risdate>2014</risdate><volume>45</volume><issue>3</issue><spage>410</spage><epage>416</epage><pages>410-416</pages><issn>1355-557X</issn><eissn>1365-2109</eissn><abstract>The Japanese ornamental (koi) carp is a popular decorative fish all over the world. In koi, clones have not yet been obtained, although production of fish with identical colour patterns could be of commercial interest. Mitotic gynogenetic progenies are essential for subsequent production of clones in fish. However, resulting late‐shocked progenies may be contaminated with meiotic gynogens from spontaneous suppression of the second meiotic division in eggs. In this study, microsatellite DNA markers were used to confirm mitotic gynogenetic origin of obtained late‐shocked progenies. Recombination rate (y) and mapping distance relative to centromere (M‐C) of 10 microsatellite loci were determined based on percentage of heterozygotes in meiotic gynogenetic progenies. The range of y varied from 0.01 to 0.96 and the M‐C map ranged from 0.5 to 48 cM. The mean value of y over the 10 loci was 0.481. Six loci, which had y 0.47 and higher, were used as markers in two late‐shocked gynogenetic progenies. 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| source | Wiley Online Library Journals Frontfile Complete |
| subjects | clones color common carp Cyprinus carpio Deoxyribonucleic acid DNA DNA markers eggs Fish fish production Freshwater Gene loci Genetic markers gynogenesis homozygosity koi loci meiosis microsatellite repeats microsatellites |
| title | Verification of mitotic gynogenesis in ornamental (koi) carp (Cyprinus carpio L.) using microsatellite DNA markers |
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