Study of glucose biosensor lifetime improvement in 37°C serum based on PANI enzyme immobilization and PLGA biodegradable membrane
Glucose sensors with long life span were fabricated and evaluated in 37°C serum to imitate in vivo conditions. Polyaniline nanofibers were electrodeposited on sensor electrodes for excessive glucose oxidase immobilization to extend the lifetime of sensors. The sensitivity of sensors stored in 37°C b...
Gespeichert in:
Veröffentlicht in: | Biosensors & bioelectronics 2014-06, Vol.56, p.91-96 |
---|---|
Hauptverfasser: | , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 96 |
---|---|
container_issue | |
container_start_page | 91 |
container_title | Biosensors & bioelectronics |
container_volume | 56 |
creator | Fang, Lu Liang, Bo Yang, Guang Hu, Yichuan Zhu, Qin Ye, Xuesong |
description | Glucose sensors with long life span were fabricated and evaluated in 37°C serum to imitate in vivo conditions. Polyaniline nanofibers were electrodeposited on sensor electrodes for excessive glucose oxidase immobilization to extend the lifetime of sensors. The sensitivity of sensors stored in 37°C bovine serum was stable for 20 days without apparent descents, and still remained 45% of its initial value after 44 days. In order to further improve the life span of sensors, a degradable polymer film, poly lactic-co-glycolic acid (PLGA), was used as an out coating layer of glucose sensors. Results showed that the PLGA coated glucose sensors left 80% of its initial sensitivity after 44 days' storage in 37°C bovine serum. The sensors could be potentially used as long-term implantable devices for real time monitoring of glucose levels.
●Polyaniline nanofibers for excessive glucose oxidase immobilization.●PLGA polymers were introduced as a degradable out coating of the sensor.●The glucose sensor left 80% of its initial sensitivity after 44 days in 37°C serum.●We provided a compatible enzyme immobilization approach for implantable sensors.●We described a degradable member base relay-released mechanism. |
doi_str_mv | 10.1016/j.bios.2014.01.017 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1504153458</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0956566314000207</els_id><sourcerecordid>1504153458</sourcerecordid><originalsourceid>FETCH-LOGICAL-c301t-5e4f4ee048704f32cfb777d7650f0d1931e8fa2653ea6b285b7c187213541b9c3</originalsourceid><addsrcrecordid>eNp9kM1qFEEQxwdRzBp9AQ_SF8HLrv05PQteliWJgUUD6rnpj-rQy_R07J4JbI4-UZ7BJ7PHXfUmFNShfvWn6tc0rwleEUza9_uVCamsKCZ8hUkt-aRZkE6yJadMPG0WeC3apWhbdta8KGWPMZZkjZ83Z5TzDhPaLZofX8bJHVDy6LafbCqA5kwYSsqoDx7GEAGFeJfTPUQYRhQGxOTPxy0qkKeIjC7gUBrQzebTNYLh4fCbj8mEPjzoMdSRHhy62V1t5mgHt1k7bXpAEaLJeoCXzTOv-wKvTv28-XZ58XX7cbn7fHW93eyWlmEyLgVwzwEw7yTmnlHrjZTSyVZgjx1ZMwKd17QVDHRraCeMtFUGJUxwYtaWnTfvjrn1me8TlFHFUCz0fb0hTUURgTkRjIuuovSI2pxKyeDVXQ5R54MiWM3u1V7NntTsXmFSS9alN6f8yURwf1f-yK7A2xOgi9W9r8_bUP5xHcMtlTP34chBtXEfIKtiAwwWXMhgR-VS-N8dvwCygKNX</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1504153458</pqid></control><display><type>article</type><title>Study of glucose biosensor lifetime improvement in 37°C serum based on PANI enzyme immobilization and PLGA biodegradable membrane</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><creator>Fang, Lu ; Liang, Bo ; Yang, Guang ; Hu, Yichuan ; Zhu, Qin ; Ye, Xuesong</creator><creatorcontrib>Fang, Lu ; Liang, Bo ; Yang, Guang ; Hu, Yichuan ; Zhu, Qin ; Ye, Xuesong</creatorcontrib><description>Glucose sensors with long life span were fabricated and evaluated in 37°C serum to imitate in vivo conditions. Polyaniline nanofibers were electrodeposited on sensor electrodes for excessive glucose oxidase immobilization to extend the lifetime of sensors. The sensitivity of sensors stored in 37°C bovine serum was stable for 20 days without apparent descents, and still remained 45% of its initial value after 44 days. In order to further improve the life span of sensors, a degradable polymer film, poly lactic-co-glycolic acid (PLGA), was used as an out coating layer of glucose sensors. Results showed that the PLGA coated glucose sensors left 80% of its initial sensitivity after 44 days' storage in 37°C bovine serum. The sensors could be potentially used as long-term implantable devices for real time monitoring of glucose levels.
●Polyaniline nanofibers for excessive glucose oxidase immobilization.●PLGA polymers were introduced as a degradable out coating of the sensor.●The glucose sensor left 80% of its initial sensitivity after 44 days in 37°C serum.●We provided a compatible enzyme immobilization approach for implantable sensors.●We described a degradable member base relay-released mechanism.</description><identifier>ISSN: 0956-5663</identifier><identifier>EISSN: 1873-4235</identifier><identifier>DOI: 10.1016/j.bios.2014.01.017</identifier><identifier>PMID: 24480128</identifier><language>eng</language><publisher>Kidlington: Elsevier B.V</publisher><subject>Aniline Compounds - chemistry ; Animals ; Aspergillus niger - enzymology ; Biological and medical sciences ; Biosensing Techniques - instrumentation ; Biosensors ; Biotechnology ; Blood Glucose - analysis ; Blood Glucose - metabolism ; Body Temperature ; Bovine serum ; Cattle ; Enzymes, Immobilized - chemistry ; Enzymes, Immobilized - metabolism ; Equipment Design ; Fundamental and applied biological sciences. Psychology ; General aspects ; Glucose Oxidase - chemistry ; Glucose Oxidase - metabolism ; Glucose sensor ; Immobilization techniques ; Lactic Acid - chemistry ; Membranes, Artificial ; Methods. Procedures. Technologies ; Nanofibers - chemistry ; Nanofibers - ultrastructure ; PANI ; PLGA ; Polyglycolic Acid - chemistry ; Various methods and equipments</subject><ispartof>Biosensors & bioelectronics, 2014-06, Vol.56, p.91-96</ispartof><rights>2014 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2014 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c301t-5e4f4ee048704f32cfb777d7650f0d1931e8fa2653ea6b285b7c187213541b9c3</citedby><cites>FETCH-LOGICAL-c301t-5e4f4ee048704f32cfb777d7650f0d1931e8fa2653ea6b285b7c187213541b9c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bios.2014.01.017$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=28306278$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24480128$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fang, Lu</creatorcontrib><creatorcontrib>Liang, Bo</creatorcontrib><creatorcontrib>Yang, Guang</creatorcontrib><creatorcontrib>Hu, Yichuan</creatorcontrib><creatorcontrib>Zhu, Qin</creatorcontrib><creatorcontrib>Ye, Xuesong</creatorcontrib><title>Study of glucose biosensor lifetime improvement in 37°C serum based on PANI enzyme immobilization and PLGA biodegradable membrane</title><title>Biosensors & bioelectronics</title><addtitle>Biosens Bioelectron</addtitle><description>Glucose sensors with long life span were fabricated and evaluated in 37°C serum to imitate in vivo conditions. Polyaniline nanofibers were electrodeposited on sensor electrodes for excessive glucose oxidase immobilization to extend the lifetime of sensors. The sensitivity of sensors stored in 37°C bovine serum was stable for 20 days without apparent descents, and still remained 45% of its initial value after 44 days. In order to further improve the life span of sensors, a degradable polymer film, poly lactic-co-glycolic acid (PLGA), was used as an out coating layer of glucose sensors. Results showed that the PLGA coated glucose sensors left 80% of its initial sensitivity after 44 days' storage in 37°C bovine serum. The sensors could be potentially used as long-term implantable devices for real time monitoring of glucose levels.
●Polyaniline nanofibers for excessive glucose oxidase immobilization.●PLGA polymers were introduced as a degradable out coating of the sensor.●The glucose sensor left 80% of its initial sensitivity after 44 days in 37°C serum.●We provided a compatible enzyme immobilization approach for implantable sensors.●We described a degradable member base relay-released mechanism.</description><subject>Aniline Compounds - chemistry</subject><subject>Animals</subject><subject>Aspergillus niger - enzymology</subject><subject>Biological and medical sciences</subject><subject>Biosensing Techniques - instrumentation</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Blood Glucose - analysis</subject><subject>Blood Glucose - metabolism</subject><subject>Body Temperature</subject><subject>Bovine serum</subject><subject>Cattle</subject><subject>Enzymes, Immobilized - chemistry</subject><subject>Enzymes, Immobilized - metabolism</subject><subject>Equipment Design</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>Glucose Oxidase - chemistry</subject><subject>Glucose Oxidase - metabolism</subject><subject>Glucose sensor</subject><subject>Immobilization techniques</subject><subject>Lactic Acid - chemistry</subject><subject>Membranes, Artificial</subject><subject>Methods. Procedures. Technologies</subject><subject>Nanofibers - chemistry</subject><subject>Nanofibers - ultrastructure</subject><subject>PANI</subject><subject>PLGA</subject><subject>Polyglycolic Acid - chemistry</subject><subject>Various methods and equipments</subject><issn>0956-5663</issn><issn>1873-4235</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM1qFEEQxwdRzBp9AQ_SF8HLrv05PQteliWJgUUD6rnpj-rQy_R07J4JbI4-UZ7BJ7PHXfUmFNShfvWn6tc0rwleEUza9_uVCamsKCZ8hUkt-aRZkE6yJadMPG0WeC3apWhbdta8KGWPMZZkjZ83Z5TzDhPaLZofX8bJHVDy6LafbCqA5kwYSsqoDx7GEAGFeJfTPUQYRhQGxOTPxy0qkKeIjC7gUBrQzebTNYLh4fCbj8mEPjzoMdSRHhy62V1t5mgHt1k7bXpAEaLJeoCXzTOv-wKvTv28-XZ58XX7cbn7fHW93eyWlmEyLgVwzwEw7yTmnlHrjZTSyVZgjx1ZMwKd17QVDHRraCeMtFUGJUxwYtaWnTfvjrn1me8TlFHFUCz0fb0hTUURgTkRjIuuovSI2pxKyeDVXQ5R54MiWM3u1V7NntTsXmFSS9alN6f8yURwf1f-yK7A2xOgi9W9r8_bUP5xHcMtlTP34chBtXEfIKtiAwwWXMhgR-VS-N8dvwCygKNX</recordid><startdate>20140615</startdate><enddate>20140615</enddate><creator>Fang, Lu</creator><creator>Liang, Bo</creator><creator>Yang, Guang</creator><creator>Hu, Yichuan</creator><creator>Zhu, Qin</creator><creator>Ye, Xuesong</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20140615</creationdate><title>Study of glucose biosensor lifetime improvement in 37°C serum based on PANI enzyme immobilization and PLGA biodegradable membrane</title><author>Fang, Lu ; Liang, Bo ; Yang, Guang ; Hu, Yichuan ; Zhu, Qin ; Ye, Xuesong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c301t-5e4f4ee048704f32cfb777d7650f0d1931e8fa2653ea6b285b7c187213541b9c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Aniline Compounds - chemistry</topic><topic>Animals</topic><topic>Aspergillus niger - enzymology</topic><topic>Biological and medical sciences</topic><topic>Biosensing Techniques - instrumentation</topic><topic>Biosensors</topic><topic>Biotechnology</topic><topic>Blood Glucose - analysis</topic><topic>Blood Glucose - metabolism</topic><topic>Body Temperature</topic><topic>Bovine serum</topic><topic>Cattle</topic><topic>Enzymes, Immobilized - chemistry</topic><topic>Enzymes, Immobilized - metabolism</topic><topic>Equipment Design</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects</topic><topic>Glucose Oxidase - chemistry</topic><topic>Glucose Oxidase - metabolism</topic><topic>Glucose sensor</topic><topic>Immobilization techniques</topic><topic>Lactic Acid - chemistry</topic><topic>Membranes, Artificial</topic><topic>Methods. Procedures. Technologies</topic><topic>Nanofibers - chemistry</topic><topic>Nanofibers - ultrastructure</topic><topic>PANI</topic><topic>PLGA</topic><topic>Polyglycolic Acid - chemistry</topic><topic>Various methods and equipments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fang, Lu</creatorcontrib><creatorcontrib>Liang, Bo</creatorcontrib><creatorcontrib>Yang, Guang</creatorcontrib><creatorcontrib>Hu, Yichuan</creatorcontrib><creatorcontrib>Zhu, Qin</creatorcontrib><creatorcontrib>Ye, Xuesong</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biosensors & bioelectronics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fang, Lu</au><au>Liang, Bo</au><au>Yang, Guang</au><au>Hu, Yichuan</au><au>Zhu, Qin</au><au>Ye, Xuesong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Study of glucose biosensor lifetime improvement in 37°C serum based on PANI enzyme immobilization and PLGA biodegradable membrane</atitle><jtitle>Biosensors & bioelectronics</jtitle><addtitle>Biosens Bioelectron</addtitle><date>2014-06-15</date><risdate>2014</risdate><volume>56</volume><spage>91</spage><epage>96</epage><pages>91-96</pages><issn>0956-5663</issn><eissn>1873-4235</eissn><abstract>Glucose sensors with long life span were fabricated and evaluated in 37°C serum to imitate in vivo conditions. Polyaniline nanofibers were electrodeposited on sensor electrodes for excessive glucose oxidase immobilization to extend the lifetime of sensors. The sensitivity of sensors stored in 37°C bovine serum was stable for 20 days without apparent descents, and still remained 45% of its initial value after 44 days. In order to further improve the life span of sensors, a degradable polymer film, poly lactic-co-glycolic acid (PLGA), was used as an out coating layer of glucose sensors. Results showed that the PLGA coated glucose sensors left 80% of its initial sensitivity after 44 days' storage in 37°C bovine serum. The sensors could be potentially used as long-term implantable devices for real time monitoring of glucose levels.
●Polyaniline nanofibers for excessive glucose oxidase immobilization.●PLGA polymers were introduced as a degradable out coating of the sensor.●The glucose sensor left 80% of its initial sensitivity after 44 days in 37°C serum.●We provided a compatible enzyme immobilization approach for implantable sensors.●We described a degradable member base relay-released mechanism.</abstract><cop>Kidlington</cop><pub>Elsevier B.V</pub><pmid>24480128</pmid><doi>10.1016/j.bios.2014.01.017</doi><tpages>6</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0956-5663 |
ispartof | Biosensors & bioelectronics, 2014-06, Vol.56, p.91-96 |
issn | 0956-5663 1873-4235 |
language | eng |
recordid | cdi_proquest_miscellaneous_1504153458 |
source | MEDLINE; Elsevier ScienceDirect Journals Complete |
subjects | Aniline Compounds - chemistry Animals Aspergillus niger - enzymology Biological and medical sciences Biosensing Techniques - instrumentation Biosensors Biotechnology Blood Glucose - analysis Blood Glucose - metabolism Body Temperature Bovine serum Cattle Enzymes, Immobilized - chemistry Enzymes, Immobilized - metabolism Equipment Design Fundamental and applied biological sciences. Psychology General aspects Glucose Oxidase - chemistry Glucose Oxidase - metabolism Glucose sensor Immobilization techniques Lactic Acid - chemistry Membranes, Artificial Methods. Procedures. Technologies Nanofibers - chemistry Nanofibers - ultrastructure PANI PLGA Polyglycolic Acid - chemistry Various methods and equipments |
title | Study of glucose biosensor lifetime improvement in 37°C serum based on PANI enzyme immobilization and PLGA biodegradable membrane |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T20%3A52%3A55IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Study%20of%20glucose%20biosensor%20lifetime%20improvement%20in%2037%C2%B0C%20serum%20based%20on%20PANI%20enzyme%20immobilization%20and%20PLGA%20biodegradable%20membrane&rft.jtitle=Biosensors%20&%20bioelectronics&rft.au=Fang,%20Lu&rft.date=2014-06-15&rft.volume=56&rft.spage=91&rft.epage=96&rft.pages=91-96&rft.issn=0956-5663&rft.eissn=1873-4235&rft_id=info:doi/10.1016/j.bios.2014.01.017&rft_dat=%3Cproquest_cross%3E1504153458%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1504153458&rft_id=info:pmid/24480128&rft_els_id=S0956566314000207&rfr_iscdi=true |