Expression, purification and substrate specificities of 3-nitrotoluene dioxygenase from Diaphorobacter sp. strain DS2

•3NTDO components were expressed, purified to homogeneity and characterized.•Reconstituted 3NTDO was in vitro active and removed nitro group efficiently.•3NTDO specificity was highest for 3-NT whereas least for 2,4-DNT.•Methylcatechol formation from 3-NT reveals its degradation pathway in D. sp. DS2...

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Veröffentlicht in:	Biochemical and biophysical research communications 2014-02, Vol.445 (1), p.36-42
Hauptverfasser:	Singh, Deepak, Kumari, Archana, Ramaswamy, S., Ramanathan, Gurunath
Format:	Artikel
Sprache:	eng
Schlagworte:	3-Nitotoluene dioxygenase (3-NTDO) Bacterial Proteins - genetics Bacterial Proteins - metabolism Biodegradation Comamonadaceae - enzymology Comamonadaceae - genetics Comamonadaceae - metabolism Diaphorobacter Dinitrobenzenes - chemistry Dinitrobenzenes - metabolism Dioxygenases - genetics Dioxygenases - metabolism Electrophoresis, Polyacrylamide Gel Escherichia coli - genetics Ferredoxins - genetics Ferredoxins - metabolism Gene Expression Regulation, Bacterial Gene Expression Regulation, Enzymologic Hydrocarbons, Aromatic - chemistry Hydrocarbons, Aromatic - metabolism Molecular Structure Nitrotoluene (NT) Oxidoreductases - genetics Oxidoreductases - metabolism Oxygenases - genetics Oxygenases - metabolism Protein Subunits - genetics Protein Subunits - metabolism Recombinant Proteins - isolation & purification Recombinant Proteins - metabolism Rieske dioxygenase Substrate Specificity Toluene - analogs & derivatives Toluene - chemistry Toluene - metabolism
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