High-throughput simultaneous genotyping of human platelet antigen-1 to -16 by using suspension array
Background Comprehensive and accurate detection of human platelet antigens (HPAs) plays a significant role in diagnosis and prevention of the platelet (PLT) alloimmune syndromes and ensuring clinical safety of patients undergoing PLT transfusion. The majority of the available methods are incapable o...
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| Veröffentlicht in: | Transfusion (Philadelphia, Pa.) Pa.), 2013-11, Vol.53 (11), p.2722-2728 |
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| container_title | Transfusion (Philadelphia, Pa.) |
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| creator | An, Qun-Xing Li, Cui-Ying Xu, Li-Juan Zhang, Xian-Qing Bai, Yan-Jun Shao, Zhong-Jun Zhang, Wei |
| description | Background
Comprehensive and accurate detection of human platelet antigens (HPAs) plays a significant role in diagnosis and prevention of the platelet (PLT) alloimmune syndromes and ensuring clinical safety of patients undergoing PLT transfusion. The majority of the available methods are incapable of performing high‐throughput simultaneous detection of HPA‐1 to ‐16, and the accuracy of many methods needs to be further enhanced.
Study Design and Methods
We have developed a new HPA‐genotyping method for simultaneous detection of HPA‐1 to ‐16 based on suspension array technology. A total of 216 samples from Chinese Han donors in Xi'an were genotyped using the developed method, and all the samples again were genotyped using polymerase chain reaction (PCR) sequence‐based typing (PCR‐SBT), which is considered the gold standard.
Results
All 216 samples were successfully genotyped for HPA‐1 to ‐16 using both our method and PCR‐SBT. Results showed that the genotype and allele frequencies obtained using our method were fully consistent with those obtained using PCR‐SBT.
Conclusion
Our method provides accurate, high‐throughput, and simultaneous genotyping of HPA‐1 to ‐16 and will serve as the foundation for large‐scale clinical genotyping of HPAs and for the establishment of an HPA‐typed PLT donor registry. |
| doi_str_mv | 10.1111/trf.12164 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1504153020</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1504153020</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4214-618830cbeb9e84683ad53a357781fc4a5fbae25775e432996fb937b78599ae5d3</originalsourceid><addsrcrecordid>eNp10V1r1TAYB_AgijubXvgFJCCDeZEtL03TXkrZzoTjRJl4GdKe9JzMNql5QfvtzezZBGG5CYFfnifPPwC8Ific5HURfX9OKCmLZ2BFOBOI1jV_DlYYFwQRwugROA7hDmNMa0xegiPKOMclFyuwvTa7PYp779JuP6UIgxnTEJXVLgW409bFeTJ2B10P92lUFk6DinrQESobTQaIwOggIiVsZ5jCvQ0pTNoG4yxU3qv5FXjRqyHo14f9BHy7urxtrtHm8_pj82GDuoKSApWkqhjuWt3WuirKiqktZ4pxISrSd4Xifas0zUeuC5ZHLPu2ZqIVFa9rpfmWnYCzpe7k3c-kQ5SjCZ0ehmUcSXgOhDNMcabv_qN3LnmbXydJIWgm2Wb1flGddyF43cvJm1H5WRIs76OXOXr5N_ps3x4qpnbU20f5kHUGpwegQqeG3ivbmfDPVZiL_G_ZXSzulxn0_HRHefv16qE1Wm6YEPXvxxvK_5ClYILL7zdr2fD1utk0X-Qn9geIaqii</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1472020504</pqid></control><display><type>article</type><title>High-throughput simultaneous genotyping of human platelet antigen-1 to -16 by using suspension array</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>An, Qun-Xing ; Li, Cui-Ying ; Xu, Li-Juan ; Zhang, Xian-Qing ; Bai, Yan-Jun ; Shao, Zhong-Jun ; Zhang, Wei</creator><creatorcontrib>An, Qun-Xing ; Li, Cui-Ying ; Xu, Li-Juan ; Zhang, Xian-Qing ; Bai, Yan-Jun ; Shao, Zhong-Jun ; Zhang, Wei</creatorcontrib><description>Background
Comprehensive and accurate detection of human platelet antigens (HPAs) plays a significant role in diagnosis and prevention of the platelet (PLT) alloimmune syndromes and ensuring clinical safety of patients undergoing PLT transfusion. The majority of the available methods are incapable of performing high‐throughput simultaneous detection of HPA‐1 to ‐16, and the accuracy of many methods needs to be further enhanced.
Study Design and Methods
We have developed a new HPA‐genotyping method for simultaneous detection of HPA‐1 to ‐16 based on suspension array technology. A total of 216 samples from Chinese Han donors in Xi'an were genotyped using the developed method, and all the samples again were genotyped using polymerase chain reaction (PCR) sequence‐based typing (PCR‐SBT), which is considered the gold standard.
Results
All 216 samples were successfully genotyped for HPA‐1 to ‐16 using both our method and PCR‐SBT. Results showed that the genotype and allele frequencies obtained using our method were fully consistent with those obtained using PCR‐SBT.
Conclusion
Our method provides accurate, high‐throughput, and simultaneous genotyping of HPA‐1 to ‐16 and will serve as the foundation for large‐scale clinical genotyping of HPAs and for the establishment of an HPA‐typed PLT donor registry.</description><identifier>ISSN: 0041-1132</identifier><identifier>EISSN: 1537-2995</identifier><identifier>DOI: 10.1111/trf.12164</identifier><identifier>PMID: 23550657</identifier><identifier>CODEN: TRANAT</identifier><language>eng</language><publisher>Hoboken, NJ: Blackwell Publishing Ltd</publisher><subject>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy ; Antigens, Human Platelet - genetics ; Base Sequence ; Biological and medical sciences ; Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis ; Genotype ; High-Throughput Screening Assays ; Humans ; Medical sciences ; Methods ; Microbiology ; Molecular Sequence Data ; Platelet Transfusion ; Polymerase Chain Reaction ; Suspensions ; Transfusions. Complications. Transfusion reactions. Cell and gene therapy</subject><ispartof>Transfusion (Philadelphia, Pa.), 2013-11, Vol.53 (11), p.2722-2728</ispartof><rights>2013 American Association of Blood Banks</rights><rights>2015 INIST-CNRS</rights><rights>2013 American Association of Blood Banks.</rights><rights>Copyright © 2013 AABB</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4214-618830cbeb9e84683ad53a357781fc4a5fbae25775e432996fb937b78599ae5d3</citedby><cites>FETCH-LOGICAL-c4214-618830cbeb9e84683ad53a357781fc4a5fbae25775e432996fb937b78599ae5d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Ftrf.12164$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Ftrf.12164$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=28057995$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23550657$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>An, Qun-Xing</creatorcontrib><creatorcontrib>Li, Cui-Ying</creatorcontrib><creatorcontrib>Xu, Li-Juan</creatorcontrib><creatorcontrib>Zhang, Xian-Qing</creatorcontrib><creatorcontrib>Bai, Yan-Jun</creatorcontrib><creatorcontrib>Shao, Zhong-Jun</creatorcontrib><creatorcontrib>Zhang, Wei</creatorcontrib><title>High-throughput simultaneous genotyping of human platelet antigen-1 to -16 by using suspension array</title><title>Transfusion (Philadelphia, Pa.)</title><addtitle>Transfusion</addtitle><description>Background
Comprehensive and accurate detection of human platelet antigens (HPAs) plays a significant role in diagnosis and prevention of the platelet (PLT) alloimmune syndromes and ensuring clinical safety of patients undergoing PLT transfusion. The majority of the available methods are incapable of performing high‐throughput simultaneous detection of HPA‐1 to ‐16, and the accuracy of many methods needs to be further enhanced.
Study Design and Methods
We have developed a new HPA‐genotyping method for simultaneous detection of HPA‐1 to ‐16 based on suspension array technology. A total of 216 samples from Chinese Han donors in Xi'an were genotyped using the developed method, and all the samples again were genotyped using polymerase chain reaction (PCR) sequence‐based typing (PCR‐SBT), which is considered the gold standard.
Results
All 216 samples were successfully genotyped for HPA‐1 to ‐16 using both our method and PCR‐SBT. Results showed that the genotype and allele frequencies obtained using our method were fully consistent with those obtained using PCR‐SBT.
Conclusion
Our method provides accurate, high‐throughput, and simultaneous genotyping of HPA‐1 to ‐16 and will serve as the foundation for large‐scale clinical genotyping of HPAs and for the establishment of an HPA‐typed PLT donor registry.</description><subject>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</subject><subject>Antigens, Human Platelet - genetics</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis</subject><subject>Genotype</subject><subject>High-Throughput Screening Assays</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Methods</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Platelet Transfusion</subject><subject>Polymerase Chain Reaction</subject><subject>Suspensions</subject><subject>Transfusions. Complications. Transfusion reactions. Cell and gene therapy</subject><issn>0041-1132</issn><issn>1537-2995</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10V1r1TAYB_AgijubXvgFJCCDeZEtL03TXkrZzoTjRJl4GdKe9JzMNql5QfvtzezZBGG5CYFfnifPPwC8Ific5HURfX9OKCmLZ2BFOBOI1jV_DlYYFwQRwugROA7hDmNMa0xegiPKOMclFyuwvTa7PYp779JuP6UIgxnTEJXVLgW409bFeTJ2B10P92lUFk6DinrQESobTQaIwOggIiVsZ5jCvQ0pTNoG4yxU3qv5FXjRqyHo14f9BHy7urxtrtHm8_pj82GDuoKSApWkqhjuWt3WuirKiqktZ4pxISrSd4Xifas0zUeuC5ZHLPu2ZqIVFa9rpfmWnYCzpe7k3c-kQ5SjCZ0ehmUcSXgOhDNMcabv_qN3LnmbXydJIWgm2Wb1flGddyF43cvJm1H5WRIs76OXOXr5N_ps3x4qpnbU20f5kHUGpwegQqeG3ivbmfDPVZiL_G_ZXSzulxn0_HRHefv16qE1Wm6YEPXvxxvK_5ClYILL7zdr2fD1utk0X-Qn9geIaqii</recordid><startdate>201311</startdate><enddate>201311</enddate><creator>An, Qun-Xing</creator><creator>Li, Cui-Ying</creator><creator>Xu, Li-Juan</creator><creator>Zhang, Xian-Qing</creator><creator>Bai, Yan-Jun</creator><creator>Shao, Zhong-Jun</creator><creator>Zhang, Wei</creator><general>Blackwell Publishing Ltd</general><general>Wiley</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>201311</creationdate><title>High-throughput simultaneous genotyping of human platelet antigen-1 to -16 by using suspension array</title><author>An, Qun-Xing ; Li, Cui-Ying ; Xu, Li-Juan ; Zhang, Xian-Qing ; Bai, Yan-Jun ; Shao, Zhong-Jun ; Zhang, Wei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4214-618830cbeb9e84683ad53a357781fc4a5fbae25775e432996fb937b78599ae5d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</topic><topic>Antigens, Human Platelet - genetics</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis</topic><topic>Genotype</topic><topic>High-Throughput Screening Assays</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Methods</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Platelet Transfusion</topic><topic>Polymerase Chain Reaction</topic><topic>Suspensions</topic><topic>Transfusions. Complications. Transfusion reactions. Cell and gene therapy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>An, Qun-Xing</creatorcontrib><creatorcontrib>Li, Cui-Ying</creatorcontrib><creatorcontrib>Xu, Li-Juan</creatorcontrib><creatorcontrib>Zhang, Xian-Qing</creatorcontrib><creatorcontrib>Bai, Yan-Jun</creatorcontrib><creatorcontrib>Shao, Zhong-Jun</creatorcontrib><creatorcontrib>Zhang, Wei</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Transfusion (Philadelphia, Pa.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>An, Qun-Xing</au><au>Li, Cui-Ying</au><au>Xu, Li-Juan</au><au>Zhang, Xian-Qing</au><au>Bai, Yan-Jun</au><au>Shao, Zhong-Jun</au><au>Zhang, Wei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High-throughput simultaneous genotyping of human platelet antigen-1 to -16 by using suspension array</atitle><jtitle>Transfusion (Philadelphia, Pa.)</jtitle><addtitle>Transfusion</addtitle><date>2013-11</date><risdate>2013</risdate><volume>53</volume><issue>11</issue><spage>2722</spage><epage>2728</epage><pages>2722-2728</pages><issn>0041-1132</issn><eissn>1537-2995</eissn><coden>TRANAT</coden><abstract>Background
Comprehensive and accurate detection of human platelet antigens (HPAs) plays a significant role in diagnosis and prevention of the platelet (PLT) alloimmune syndromes and ensuring clinical safety of patients undergoing PLT transfusion. The majority of the available methods are incapable of performing high‐throughput simultaneous detection of HPA‐1 to ‐16, and the accuracy of many methods needs to be further enhanced.
Study Design and Methods
We have developed a new HPA‐genotyping method for simultaneous detection of HPA‐1 to ‐16 based on suspension array technology. A total of 216 samples from Chinese Han donors in Xi'an were genotyped using the developed method, and all the samples again were genotyped using polymerase chain reaction (PCR) sequence‐based typing (PCR‐SBT), which is considered the gold standard.
Results
All 216 samples were successfully genotyped for HPA‐1 to ‐16 using both our method and PCR‐SBT. Results showed that the genotype and allele frequencies obtained using our method were fully consistent with those obtained using PCR‐SBT.
Conclusion
Our method provides accurate, high‐throughput, and simultaneous genotyping of HPA‐1 to ‐16 and will serve as the foundation for large‐scale clinical genotyping of HPAs and for the establishment of an HPA‐typed PLT donor registry.</abstract><cop>Hoboken, NJ</cop><pub>Blackwell Publishing Ltd</pub><pmid>23550657</pmid><doi>10.1111/trf.12164</doi><tpages>7</tpages></addata></record> |
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| ispartof | Transfusion (Philadelphia, Pa.), 2013-11, Vol.53 (11), p.2722-2728 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy Antigens, Human Platelet - genetics Base Sequence Biological and medical sciences Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis Genotype High-Throughput Screening Assays Humans Medical sciences Methods Microbiology Molecular Sequence Data Platelet Transfusion Polymerase Chain Reaction Suspensions Transfusions. Complications. Transfusion reactions. Cell and gene therapy |
| title | High-throughput simultaneous genotyping of human platelet antigen-1 to -16 by using suspension array |
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