Llama oviductal sperm reservoirs: involvement of bulbourethral glands
Summary The aim of this study was to elucidate the role of llama seminal plasma in the formation of oviductal sperm reservoirs. Female llamas with follicles in the mature phase were mated with a bulbourethral glands‐removed male. Females mated with nonbulbourethral glands‐removed males were used as...
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| Veröffentlicht in: | Andrologia 2014-04, Vol.46 (3), p.290-295 |
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| creator | Apichela, S. A. Argañaraz, M. E. Giuliano, S. Zampini, R. Carretero, I. Miragaya, M. Miceli, D. C. |
| description | Summary
The aim of this study was to elucidate the role of llama seminal plasma in the formation of oviductal sperm reservoirs. Female llamas with follicles in the mature phase were mated with a bulbourethral glands‐removed male. Females mated with nonbulbourethral glands‐removed males were used as control. Oviducts were obtained by surgery 24 h after mating. The uterotubal junction and isthmus were examined by scanning electron microscopy, and mucopolysaccharides were identified by Alcian blue staining. To know the proteins probably involved in sperm reservoir formation, SDS‐PAGE of seminal plasma (8% and 18% resolving gel) was made. Spermatozoa only adhered to the oviductal mucosa surface of uterotubal junction of females mated with nonbulbourethral glands‐removed males confirming that seminal plasma and, in particular, bulbourethral secretions are related with the oviductal sperm reservoir formation. Histological sections showed sperm in the lumen, immersed in substance, positive for acid mucopolysaccharides. Alcian blue staining of seminal plasma proteins SDS‐PAGE showed a band of high molecular weight containing mucopolysaccharides, only present in nonbulbourethral glands‐removed males. Bulbourethral glands would secrete at least eight different proteins that most likely participate in the process of sperm storage in the oviduct. |
| doi_str_mv | 10.1111/and.12080 |
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The aim of this study was to elucidate the role of llama seminal plasma in the formation of oviductal sperm reservoirs. Female llamas with follicles in the mature phase were mated with a bulbourethral glands‐removed male. Females mated with nonbulbourethral glands‐removed males were used as control. Oviducts were obtained by surgery 24 h after mating. The uterotubal junction and isthmus were examined by scanning electron microscopy, and mucopolysaccharides were identified by Alcian blue staining. To know the proteins probably involved in sperm reservoir formation, SDS‐PAGE of seminal plasma (8% and 18% resolving gel) was made. Spermatozoa only adhered to the oviductal mucosa surface of uterotubal junction of females mated with nonbulbourethral glands‐removed males confirming that seminal plasma and, in particular, bulbourethral secretions are related with the oviductal sperm reservoir formation. Histological sections showed sperm in the lumen, immersed in substance, positive for acid mucopolysaccharides. Alcian blue staining of seminal plasma proteins SDS‐PAGE showed a band of high molecular weight containing mucopolysaccharides, only present in nonbulbourethral glands‐removed males. Bulbourethral glands would secrete at least eight different proteins that most likely participate in the process of sperm storage in the oviduct.</description><identifier>ISSN: 0303-4569</identifier><identifier>EISSN: 1439-0272</identifier><identifier>DOI: 10.1111/and.12080</identifier><identifier>PMID: 23463949</identifier><language>eng</language><publisher>Germany: Blackwell Publishing Ltd</publisher><subject>Animals ; Bulbourethral Glands - anatomy & histology ; Bulbourethral Glands - physiology ; Camelids, New World - anatomy & histology ; Camelids, New World - physiology ; Fallopian Tubes - anatomy & histology ; Fallopian Tubes - physiology ; Female ; Llama ; Male ; Microscopy, Electron, Scanning ; oviduct ; Ovulation - physiology ; Reproduction - physiology ; Semen - physiology ; seminal plasma ; Seminal Plasma Proteins - physiology ; Sexual Behavior, Animal - physiology ; sperm reservoirs ; Spermatozoa - physiology ; Spermatozoa - ultrastructure</subject><ispartof>Andrologia, 2014-04, Vol.46 (3), p.290-295</ispartof><rights>2013 Blackwell Verlag GmbH</rights><rights>2013 Blackwell Verlag GmbH.</rights><rights>Copyright © 2014 Blackwell Verlag GmbH</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4260-600b1c41b6a86b1b1e84229a9987fc905d712ae2ff9bf7e3985f6058cd9589c93</citedby><cites>FETCH-LOGICAL-c4260-600b1c41b6a86b1b1e84229a9987fc905d712ae2ff9bf7e3985f6058cd9589c93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fand.12080$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fand.12080$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23463949$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Apichela, S. A.</creatorcontrib><creatorcontrib>Argañaraz, M. E.</creatorcontrib><creatorcontrib>Giuliano, S.</creatorcontrib><creatorcontrib>Zampini, R.</creatorcontrib><creatorcontrib>Carretero, I.</creatorcontrib><creatorcontrib>Miragaya, M.</creatorcontrib><creatorcontrib>Miceli, D. C.</creatorcontrib><title>Llama oviductal sperm reservoirs: involvement of bulbourethral glands</title><title>Andrologia</title><addtitle>Andrologia</addtitle><description>Summary
The aim of this study was to elucidate the role of llama seminal plasma in the formation of oviductal sperm reservoirs. Female llamas with follicles in the mature phase were mated with a bulbourethral glands‐removed male. Females mated with nonbulbourethral glands‐removed males were used as control. Oviducts were obtained by surgery 24 h after mating. The uterotubal junction and isthmus were examined by scanning electron microscopy, and mucopolysaccharides were identified by Alcian blue staining. To know the proteins probably involved in sperm reservoir formation, SDS‐PAGE of seminal plasma (8% and 18% resolving gel) was made. Spermatozoa only adhered to the oviductal mucosa surface of uterotubal junction of females mated with nonbulbourethral glands‐removed males confirming that seminal plasma and, in particular, bulbourethral secretions are related with the oviductal sperm reservoir formation. Histological sections showed sperm in the lumen, immersed in substance, positive for acid mucopolysaccharides. Alcian blue staining of seminal plasma proteins SDS‐PAGE showed a band of high molecular weight containing mucopolysaccharides, only present in nonbulbourethral glands‐removed males. Bulbourethral glands would secrete at least eight different proteins that most likely participate in the process of sperm storage in the oviduct.</description><subject>Animals</subject><subject>Bulbourethral Glands - anatomy & histology</subject><subject>Bulbourethral Glands - physiology</subject><subject>Camelids, New World - anatomy & histology</subject><subject>Camelids, New World - physiology</subject><subject>Fallopian Tubes - anatomy & histology</subject><subject>Fallopian Tubes - physiology</subject><subject>Female</subject><subject>Llama</subject><subject>Male</subject><subject>Microscopy, Electron, Scanning</subject><subject>oviduct</subject><subject>Ovulation - physiology</subject><subject>Reproduction - physiology</subject><subject>Semen - physiology</subject><subject>seminal plasma</subject><subject>Seminal Plasma Proteins - physiology</subject><subject>Sexual Behavior, Animal - physiology</subject><subject>sperm reservoirs</subject><subject>Spermatozoa - physiology</subject><subject>Spermatozoa - ultrastructure</subject><issn>0303-4569</issn><issn>1439-0272</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kDtPw0AQhE8IBFFIwR9AlmigcHJv--hQCAERQEg8ytPZXoPBjsOdbci_5yCEAolttvl2dmYQ2iN4SPyMzDwbEopjvIF6hDMVYhrRTdTDDLOQC6l20MC5F-yHiyjifBvtUMYlU1z10GRWmsoEdVdkbdqYMnALsFVgwYHt6sK646CYd3XZQQXzJqjzIGnLpG4tNM_W40-l_-920VZuSgeDn91H92eTu_F5OLuZXoxPZmHKqcShxDghKSeJNLFMSEIg5pQqo1Qc5anCIosINUDzXCV5BEzFIpdYxGmmRKxSxfrocKW7sPVbC67RVeFSKL0JqFunicCc-JSCevTgD_riXc-9uy-KCcpjKjx1tKJSWztnIdcLW1TGLjXB-qte7ePp73o9u_-j2CYVZL_kukwPjFbAe1HC8n8lfXJ9upYMVxeFa-Dj98LYVy0jFgn9eD3V04eH2d2VvNWX7BNnbJGs</recordid><startdate>201404</startdate><enddate>201404</enddate><creator>Apichela, S. A.</creator><creator>Argañaraz, M. E.</creator><creator>Giuliano, S.</creator><creator>Zampini, R.</creator><creator>Carretero, I.</creator><creator>Miragaya, M.</creator><creator>Miceli, D. C.</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>201404</creationdate><title>Llama oviductal sperm reservoirs: involvement of bulbourethral glands</title><author>Apichela, S. A. ; Argañaraz, M. E. ; Giuliano, S. ; Zampini, R. ; Carretero, I. ; Miragaya, M. ; Miceli, D. C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4260-600b1c41b6a86b1b1e84229a9987fc905d712ae2ff9bf7e3985f6058cd9589c93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Bulbourethral Glands - anatomy & histology</topic><topic>Bulbourethral Glands - physiology</topic><topic>Camelids, New World - anatomy & histology</topic><topic>Camelids, New World - physiology</topic><topic>Fallopian Tubes - anatomy & histology</topic><topic>Fallopian Tubes - physiology</topic><topic>Female</topic><topic>Llama</topic><topic>Male</topic><topic>Microscopy, Electron, Scanning</topic><topic>oviduct</topic><topic>Ovulation - physiology</topic><topic>Reproduction - physiology</topic><topic>Semen - physiology</topic><topic>seminal plasma</topic><topic>Seminal Plasma Proteins - physiology</topic><topic>Sexual Behavior, Animal - physiology</topic><topic>sperm reservoirs</topic><topic>Spermatozoa - physiology</topic><topic>Spermatozoa - ultrastructure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Apichela, S. A.</creatorcontrib><creatorcontrib>Argañaraz, M. E.</creatorcontrib><creatorcontrib>Giuliano, S.</creatorcontrib><creatorcontrib>Zampini, R.</creatorcontrib><creatorcontrib>Carretero, I.</creatorcontrib><creatorcontrib>Miragaya, M.</creatorcontrib><creatorcontrib>Miceli, D. C.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Andrologia</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Apichela, S. A.</au><au>Argañaraz, M. E.</au><au>Giuliano, S.</au><au>Zampini, R.</au><au>Carretero, I.</au><au>Miragaya, M.</au><au>Miceli, D. C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Llama oviductal sperm reservoirs: involvement of bulbourethral glands</atitle><jtitle>Andrologia</jtitle><addtitle>Andrologia</addtitle><date>2014-04</date><risdate>2014</risdate><volume>46</volume><issue>3</issue><spage>290</spage><epage>295</epage><pages>290-295</pages><issn>0303-4569</issn><eissn>1439-0272</eissn><abstract>Summary
The aim of this study was to elucidate the role of llama seminal plasma in the formation of oviductal sperm reservoirs. Female llamas with follicles in the mature phase were mated with a bulbourethral glands‐removed male. Females mated with nonbulbourethral glands‐removed males were used as control. Oviducts were obtained by surgery 24 h after mating. The uterotubal junction and isthmus were examined by scanning electron microscopy, and mucopolysaccharides were identified by Alcian blue staining. To know the proteins probably involved in sperm reservoir formation, SDS‐PAGE of seminal plasma (8% and 18% resolving gel) was made. Spermatozoa only adhered to the oviductal mucosa surface of uterotubal junction of females mated with nonbulbourethral glands‐removed males confirming that seminal plasma and, in particular, bulbourethral secretions are related with the oviductal sperm reservoir formation. Histological sections showed sperm in the lumen, immersed in substance, positive for acid mucopolysaccharides. Alcian blue staining of seminal plasma proteins SDS‐PAGE showed a band of high molecular weight containing mucopolysaccharides, only present in nonbulbourethral glands‐removed males. Bulbourethral glands would secrete at least eight different proteins that most likely participate in the process of sperm storage in the oviduct.</abstract><cop>Germany</cop><pub>Blackwell Publishing Ltd</pub><pmid>23463949</pmid><doi>10.1111/and.12080</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Bulbourethral Glands - anatomy & histology Bulbourethral Glands - physiology Camelids, New World - anatomy & histology Camelids, New World - physiology Fallopian Tubes - anatomy & histology Fallopian Tubes - physiology Female Llama Male Microscopy, Electron, Scanning oviduct Ovulation - physiology Reproduction - physiology Semen - physiology seminal plasma Seminal Plasma Proteins - physiology Sexual Behavior, Animal - physiology sperm reservoirs Spermatozoa - physiology Spermatozoa - ultrastructure |
| title | Llama oviductal sperm reservoirs: involvement of bulbourethral glands |
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