Primary Structure, Synthesis, and Biological Activity of Rat Endothelin, an Endothelium-Derived Vasoconstrictor Peptide
Endothelin is a potent vasoconstrictor/pressor peptide, which we recently characterized from the conditioned culture medium of porcine aortic endothelial cells. We report here the cloning and partial sequencing of the rat endothelin gene. The nucleotide sequence predicted a 21-residue peptide simila...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1988-09, Vol.85 (18), p.6964-6967 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 85 |
| creator | Yanagisawa, Masashi Inoue, Akihiro Ishikawa, Tomohisa Kasuya, Yoshitoshi Kimura, Sadao Kumagaye, Shin-Ichiroh Nakajima, Kiichiroh Watanabe, Takushi X. Sakakibara, Shumpei Goto, Katsutoshi Masaki, Tomoh |
| description | Endothelin is a potent vasoconstrictor/pressor peptide, which we recently characterized from the conditioned culture medium of porcine aortic endothelial cells. We report here the cloning and partial sequencing of the rat endothelin gene. The nucleotide sequence predicted a 21-residue peptide similar to, but distinct from, porcine endothelin; 15 residues of rat endothelin were identical and 3 residues were substitutions by chemically similar amino acid residues to those in the porcine peptide. Synthetic rat endothelin was then prepared according to its deduced amino acid sequence. This synthetic peptide had (i) potent vasoconstrictor activity in the rat aortic strip and in perfused rat heart and (ii) a characteristically long-lasting in vivo pressor activity by intraaortic bolus injection in the conscious rat. |
| doi_str_mv | 10.1073/pnas.85.18.6964 |
| format | Article |
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We report here the cloning and partial sequencing of the rat endothelin gene. The nucleotide sequence predicted a 21-residue peptide similar to, but distinct from, porcine endothelin; 15 residues of rat endothelin were identical and 3 residues were substitutions by chemically similar amino acid residues to those in the porcine peptide. Synthetic rat endothelin was then prepared according to its deduced amino acid sequence. 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We report here the cloning and partial sequencing of the rat endothelin gene. The nucleotide sequence predicted a 21-residue peptide similar to, but distinct from, porcine endothelin; 15 residues of rat endothelin were identical and 3 residues were substitutions by chemically similar amino acid residues to those in the porcine peptide. Synthetic rat endothelin was then prepared according to its deduced amino acid sequence. This synthetic peptide had (i) potent vasoconstrictor activity in the rat aortic strip and in perfused rat heart and (ii) a characteristically long-lasting in vivo pressor activity by intraaortic bolus injection in the conscious rat.</description><subject>Amino Acid Sequence</subject><subject>Amino acids</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Blood pressure</subject><subject>Blood Pressure - drug effects</subject><subject>Boluses</subject><subject>Catheterization</subject><subject>Endothelial cells</subject><subject>Endothelins</subject><subject>Japanese culture</subject><subject>Male</subject><subject>Molecular Sequence Data</subject><subject>Nucleotides</subject><subject>Peptides - analysis</subject><subject>Peptides - genetics</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Vasoconstriction</subject><subject>Vasoconstrictors</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1v1DAQxS1EVbaFMxISyCd6abbjr9g-llIoUiUqClwtx3HAVTbe2k7L_vdNtAsVF5jLaPR-80aah9BLAksCkp2sB5uXSiyJWta65k_QgoAmVc01PEULACorxSl_hg5yvgEALRTso30GXCgqF-j-KoWVTRt8XdLoypj8Mb7eDOWnzyEfYzu0-F2IffwRnO3xqSvhLpQNjh3-Ygs-H9o4oX0YZvRxHFfVe5_CnW_xd5uji0MuKbgSE77y6xJa_xztdbbP_sWuH6JvH86_nl1Ul58_fjo7vaycAF4qL5xTwgldS8oUbaRsa68aTcECb2rbTlU3wDi04ISjzKta6U5AIzpZW8sO0dut7zrF29HnYlYhO9_3dvBxzEYqpqVm6r8gEcAEJzN4sgVdijkn35n19oWGgJkzMXMmRglDlJkzmTZe76zHZuXbP_wuhEl_s9Pnxd_qXwZH_wRMN_Z98b_KRL7akjd5evbjJSqAsgcRdaqA</recordid><startdate>19880901</startdate><enddate>19880901</enddate><creator>Yanagisawa, Masashi</creator><creator>Inoue, Akihiro</creator><creator>Ishikawa, Tomohisa</creator><creator>Kasuya, Yoshitoshi</creator><creator>Kimura, Sadao</creator><creator>Kumagaye, Shin-Ichiroh</creator><creator>Nakajima, Kiichiroh</creator><creator>Watanabe, Takushi X.</creator><creator>Sakakibara, Shumpei</creator><creator>Goto, Katsutoshi</creator><creator>Masaki, Tomoh</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19880901</creationdate><title>Primary Structure, Synthesis, and Biological Activity of Rat Endothelin, an Endothelium-Derived Vasoconstrictor Peptide</title><author>Yanagisawa, Masashi ; 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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1988-09, Vol.85 (18), p.6964-6967 |
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| source | MEDLINE; PubMed (Medline); Alma/SFX Local Collection; Free Full-Text Journals in Chemistry; JSTOR |
| subjects | Amino Acid Sequence Amino acids Animals Base Sequence Blood pressure Blood Pressure - drug effects Boluses Catheterization Endothelial cells Endothelins Japanese culture Male Molecular Sequence Data Nucleotides Peptides - analysis Peptides - genetics Rats Rats, Inbred Strains Vasoconstriction Vasoconstrictors |
| title | Primary Structure, Synthesis, and Biological Activity of Rat Endothelin, an Endothelium-Derived Vasoconstrictor Peptide |
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