Development of a simple, cost-effective, semi-correlative light and electron microscopy method to allow the immunoelectron localisation of non-uniformly distributed placental proteins
Abstract Immunoelectron microscopy is wrought with technical limitations that complicate its use. However, advances in correlative light and electron microscopy have recently lead to improvements in this field. We report the development of a semi-correlative approach to investigate the ultrastructur...
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| Veröffentlicht in: | Placenta (Eastbourne) 2014-03, Vol.35 (3), p.223-227 |
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| container_title | Placenta (Eastbourne) |
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| creator | Viall, C.A Holloway, H Chen, Q Stone, P.R Chamley, L.W |
| description | Abstract Immunoelectron microscopy is wrought with technical limitations that complicate its use. However, advances in correlative light and electron microscopy have recently lead to improvements in this field. We report the development of a semi-correlative approach to investigate the ultrastructural location of an antiphospholipid antibody within the syncytiotrophoblast. This method offers several advantages over existing methodologies, since it preserves antigenicity, shows good immunolabel penetrability and does not require specialized equipment. The use of a pre-embedding screen has also allowed us to target individual placental villi and overcome sampling limitations of the electron microscope. This simple, cost-effective method is likely to find widespread application in placental research. |
| doi_str_mv | 10.1016/j.placenta.2014.01.005 |
| format | Article |
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However, advances in correlative light and electron microscopy have recently lead to improvements in this field. We report the development of a semi-correlative approach to investigate the ultrastructural location of an antiphospholipid antibody within the syncytiotrophoblast. This method offers several advantages over existing methodologies, since it preserves antigenicity, shows good immunolabel penetrability and does not require specialized equipment. The use of a pre-embedding screen has also allowed us to target individual placental villi and overcome sampling limitations of the electron microscope. This simple, cost-effective method is likely to find widespread application in placental research.</description><identifier>ISSN: 0143-4004</identifier><identifier>EISSN: 1532-3102</identifier><identifier>DOI: 10.1016/j.placenta.2014.01.005</identifier><identifier>PMID: 24508096</identifier><identifier>CODEN: PLACDF</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>Animals ; Antibodies, Antiphospholipid - analysis ; Antiphospholipid antibody ; Biological and medical sciences ; Correlative microscopy ; Electron microscopy ; Embryology: invertebrates and vertebrates. Teratology ; Female ; Fundamental and applied biological sciences. Psychology ; Humans ; Immunoelectron ; Internal Medicine ; Microscopy, Electron - methods ; Microscopy, Immunoelectron - methods ; Obstetrics and Gynecology ; Placenta ; Pregnancy ; Pregnancy Proteins - metabolism ; Syncytiotrophoblast ; Trophoblasts - chemistry</subject><ispartof>Placenta (Eastbourne), 2014-03, Vol.35 (3), p.223-227</ispartof><rights>Elsevier Ltd</rights><rights>2014 Elsevier Ltd</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2014 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c453t-2c80b8f12b009c6c41d81eb81df8ba103aa2220718ace9f17be4717b314c09753</citedby><cites>FETCH-LOGICAL-c453t-2c80b8f12b009c6c41d81eb81df8ba103aa2220718ace9f17be4717b314c09753</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S014340041400006X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=28312623$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24508096$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Viall, C.A</creatorcontrib><creatorcontrib>Holloway, H</creatorcontrib><creatorcontrib>Chen, Q</creatorcontrib><creatorcontrib>Stone, P.R</creatorcontrib><creatorcontrib>Chamley, L.W</creatorcontrib><title>Development of a simple, cost-effective, semi-correlative light and electron microscopy method to allow the immunoelectron localisation of non-uniformly distributed placental proteins</title><title>Placenta (Eastbourne)</title><addtitle>Placenta</addtitle><description>Abstract Immunoelectron microscopy is wrought with technical limitations that complicate its use. However, advances in correlative light and electron microscopy have recently lead to improvements in this field. We report the development of a semi-correlative approach to investigate the ultrastructural location of an antiphospholipid antibody within the syncytiotrophoblast. This method offers several advantages over existing methodologies, since it preserves antigenicity, shows good immunolabel penetrability and does not require specialized equipment. The use of a pre-embedding screen has also allowed us to target individual placental villi and overcome sampling limitations of the electron microscope. This simple, cost-effective method is likely to find widespread application in placental research.</description><subject>Animals</subject><subject>Antibodies, Antiphospholipid - analysis</subject><subject>Antiphospholipid antibody</subject><subject>Biological and medical sciences</subject><subject>Correlative microscopy</subject><subject>Electron microscopy</subject><subject>Embryology: invertebrates and vertebrates. Teratology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Immunoelectron</subject><subject>Internal Medicine</subject><subject>Microscopy, Electron - methods</subject><subject>Microscopy, Immunoelectron - methods</subject><subject>Obstetrics and Gynecology</subject><subject>Placenta</subject><subject>Pregnancy</subject><subject>Pregnancy Proteins - metabolism</subject><subject>Syncytiotrophoblast</subject><subject>Trophoblasts - chemistry</subject><issn>0143-4004</issn><issn>1532-3102</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUsuu0zAQjRCIWwq_cOUNEgsSxnaSphsEujylK7EAJHaW40yoix_Bdor6ZfwejtpeJDZsxrJ95pyZM1MU1xQqCrR9sa8mIxW6JCsGtK6AVgDNvWJFG85KToHdL1b5g5c1QH1VPIpxDwDbmrKHxRWrG-hg266K32_wgMZPNlMRPxJJoraTwedE-ZhKHEdUSR_yPaLVpfIhoJHLCzH6-y4R6QaCJoOCd8RqFXxUfjoSi2nnB5I8kcb4XyTtkGhrZ-fv0MYraXTMbPmStZ135ez06IM1RzLomILu54QDufRqyBR8Qu3i4-LBKE3EJ-dzXXx99_bLzYfy9tP7jzevb0tVNzyVTHXQdyNlfe5dtaqmQ0ex7-gwdr2kwKVkjMGGdllgO9JNj_UmR05rBdtNw9fFsxNvFv45Y0zC6qjQGOnQz1HQBhjnDLpNhrYn6OJBDDiKKWgrw1FQEMvQxF5cGhHL0ARQkYeWE6_PGnNvcbhLu0wpA56eATJmy8YgndLxL67jlLW5jHXx6oTD7MhBYxBRaXQKBx2y52Lw-v-1vPyHQhntdFb9gUeMez8Hl_0WVEQmQHxeVmzZMJoDQPuN_wGUdtKq</recordid><startdate>20140301</startdate><enddate>20140301</enddate><creator>Viall, C.A</creator><creator>Holloway, H</creator><creator>Chen, Q</creator><creator>Stone, P.R</creator><creator>Chamley, L.W</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20140301</creationdate><title>Development of a simple, cost-effective, semi-correlative light and electron microscopy method to allow the immunoelectron localisation of non-uniformly distributed placental proteins</title><author>Viall, C.A ; Holloway, H ; Chen, Q ; Stone, P.R ; Chamley, L.W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c453t-2c80b8f12b009c6c41d81eb81df8ba103aa2220718ace9f17be4717b314c09753</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Antibodies, Antiphospholipid - analysis</topic><topic>Antiphospholipid antibody</topic><topic>Biological and medical sciences</topic><topic>Correlative microscopy</topic><topic>Electron microscopy</topic><topic>Embryology: invertebrates and vertebrates. Teratology</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Immunoelectron</topic><topic>Internal Medicine</topic><topic>Microscopy, Electron - methods</topic><topic>Microscopy, Immunoelectron - methods</topic><topic>Obstetrics and Gynecology</topic><topic>Placenta</topic><topic>Pregnancy</topic><topic>Pregnancy Proteins - metabolism</topic><topic>Syncytiotrophoblast</topic><topic>Trophoblasts - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Viall, C.A</creatorcontrib><creatorcontrib>Holloway, H</creatorcontrib><creatorcontrib>Chen, Q</creatorcontrib><creatorcontrib>Stone, P.R</creatorcontrib><creatorcontrib>Chamley, L.W</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Placenta (Eastbourne)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Viall, C.A</au><au>Holloway, H</au><au>Chen, Q</au><au>Stone, P.R</au><au>Chamley, L.W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of a simple, cost-effective, semi-correlative light and electron microscopy method to allow the immunoelectron localisation of non-uniformly distributed placental proteins</atitle><jtitle>Placenta (Eastbourne)</jtitle><addtitle>Placenta</addtitle><date>2014-03-01</date><risdate>2014</risdate><volume>35</volume><issue>3</issue><spage>223</spage><epage>227</epage><pages>223-227</pages><issn>0143-4004</issn><eissn>1532-3102</eissn><coden>PLACDF</coden><abstract>Abstract Immunoelectron microscopy is wrought with technical limitations that complicate its use. However, advances in correlative light and electron microscopy have recently lead to improvements in this field. We report the development of a semi-correlative approach to investigate the ultrastructural location of an antiphospholipid antibody within the syncytiotrophoblast. This method offers several advantages over existing methodologies, since it preserves antigenicity, shows good immunolabel penetrability and does not require specialized equipment. The use of a pre-embedding screen has also allowed us to target individual placental villi and overcome sampling limitations of the electron microscope. This simple, cost-effective method is likely to find widespread application in placental research.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>24508096</pmid><doi>10.1016/j.placenta.2014.01.005</doi><tpages>5</tpages></addata></record> |
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| ispartof | Placenta (Eastbourne), 2014-03, Vol.35 (3), p.223-227 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Antibodies, Antiphospholipid - analysis Antiphospholipid antibody Biological and medical sciences Correlative microscopy Electron microscopy Embryology: invertebrates and vertebrates. Teratology Female Fundamental and applied biological sciences. Psychology Humans Immunoelectron Internal Medicine Microscopy, Electron - methods Microscopy, Immunoelectron - methods Obstetrics and Gynecology Placenta Pregnancy Pregnancy Proteins - metabolism Syncytiotrophoblast Trophoblasts - chemistry |
| title | Development of a simple, cost-effective, semi-correlative light and electron microscopy method to allow the immunoelectron localisation of non-uniformly distributed placental proteins |
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