Detoxification of aflatoxin B1 by an aqueous extract from leaves of Adhatoda vasica Nees
The effectiveness of aqueous extracts of various medicinal plants in detoxification of aflatoxin B1 (AFB1) was tested in vitro by thin-layer chromatography and enzyme-linked immunosorbent assay (ELISA). Among the different plant extracts, the leaf extract of Vasaka (Adhatoda vasica Nees) showed the...
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| Veröffentlicht in: | Microbiological research 2014-04, Vol.169 (4), p.294-300 |
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| creator | Vijayanandraj, S. Brinda, R. Kannan, K. Adhithya, R. Vinothini, S. Senthil, K. Chinta, Ramakoteswara Rao Paranidharan, V. Velazhahan, R. |
| description | The effectiveness of aqueous extracts of various medicinal plants in detoxification of aflatoxin B1 (AFB1) was tested in vitro by thin-layer chromatography and enzyme-linked immunosorbent assay (ELISA). Among the different plant extracts, the leaf extract of Vasaka (Adhatoda vasica Nees) showed the maximum degradation of AFB1 (≥98%) after incubation for 24h at 37°C. The aflatoxin detoxifying activity of the A. vasica leaf extract was significantly reduced by heating to 100°C for 10min or autoclaving at 121°C for 20min. Dialysis had no effect on aflatoxin detoxifying ability of A. vasica extract and the dialyzed extract showed similar level of detoxification of AFB1 as that of the untreated extract. A time course study of aflatoxin detoxification by A. vasica extract showed that 69% of the toxin was degraded within 6h and ≥95% degradation was observed after 24h of incubation. Detoxification of AFB1 by A. vasica extract was further confirmed by liquid chromatography–mass spectrometry (LC–MS) analysis. Phytochemical analysis revealed the presence of alkaloids in methanolic extract of A. vasica leaves. A partially purified alkaloid from A. vasica leaves by preparative TLC exhibited strong AFB1 detoxification activity. |
| doi_str_mv | 10.1016/j.micres.2013.07.008 |
| format | Article |
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Among the different plant extracts, the leaf extract of Vasaka (Adhatoda vasica Nees) showed the maximum degradation of AFB1 (≥98%) after incubation for 24h at 37°C. The aflatoxin detoxifying activity of the A. vasica leaf extract was significantly reduced by heating to 100°C for 10min or autoclaving at 121°C for 20min. Dialysis had no effect on aflatoxin detoxifying ability of A. vasica extract and the dialyzed extract showed similar level of detoxification of AFB1 as that of the untreated extract. A time course study of aflatoxin detoxification by A. vasica extract showed that 69% of the toxin was degraded within 6h and ≥95% degradation was observed after 24h of incubation. Detoxification of AFB1 by A. vasica extract was further confirmed by liquid chromatography–mass spectrometry (LC–MS) analysis. Phytochemical analysis revealed the presence of alkaloids in methanolic extract of A. vasica leaves. A partially purified alkaloid from A. vasica leaves by preparative TLC exhibited strong AFB1 detoxification activity.</description><identifier>ISSN: 0944-5013</identifier><identifier>EISSN: 1618-0623</identifier><identifier>DOI: 10.1016/j.micres.2013.07.008</identifier><identifier>PMID: 23928380</identifier><language>eng</language><publisher>Germany: Elsevier GmbH</publisher><subject>Adhatoda vasica ; Aflatoxin B1 ; Aflatoxin B1 - antagonists & inhibitors ; Alkaloids - isolation & purification ; Alkaloids - metabolism ; Chromatography, Liquid ; Chromatography, Thin Layer ; Detoxification ; Enzyme-Linked Immunosorbent Assay ; Justicia - chemistry ; Kinetics ; Mass Spectrometry ; Medicinal plant ; Plant Extracts - isolation & purification ; Plant Extracts - metabolism ; Plant Leaves - chemistry ; Temperature ; Time Factors</subject><ispartof>Microbiological research, 2014-04, Vol.169 (4), p.294-300</ispartof><rights>2013 Elsevier GmbH</rights><rights>Copyright © 2013 Elsevier GmbH. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-8952c3b1791e826f4067cafbaad1427c62160c446d18843e72d59e40181e5df83</citedby><cites>FETCH-LOGICAL-c474t-8952c3b1791e826f4067cafbaad1427c62160c446d18843e72d59e40181e5df83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0944501313001183$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23928380$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vijayanandraj, S.</creatorcontrib><creatorcontrib>Brinda, R.</creatorcontrib><creatorcontrib>Kannan, K.</creatorcontrib><creatorcontrib>Adhithya, R.</creatorcontrib><creatorcontrib>Vinothini, S.</creatorcontrib><creatorcontrib>Senthil, K.</creatorcontrib><creatorcontrib>Chinta, Ramakoteswara Rao</creatorcontrib><creatorcontrib>Paranidharan, V.</creatorcontrib><creatorcontrib>Velazhahan, R.</creatorcontrib><title>Detoxification of aflatoxin B1 by an aqueous extract from leaves of Adhatoda vasica Nees</title><title>Microbiological research</title><addtitle>Microbiol Res</addtitle><description>The effectiveness of aqueous extracts of various medicinal plants in detoxification of aflatoxin B1 (AFB1) was tested in vitro by thin-layer chromatography and enzyme-linked immunosorbent assay (ELISA). Among the different plant extracts, the leaf extract of Vasaka (Adhatoda vasica Nees) showed the maximum degradation of AFB1 (≥98%) after incubation for 24h at 37°C. The aflatoxin detoxifying activity of the A. vasica leaf extract was significantly reduced by heating to 100°C for 10min or autoclaving at 121°C for 20min. Dialysis had no effect on aflatoxin detoxifying ability of A. vasica extract and the dialyzed extract showed similar level of detoxification of AFB1 as that of the untreated extract. A time course study of aflatoxin detoxification by A. vasica extract showed that 69% of the toxin was degraded within 6h and ≥95% degradation was observed after 24h of incubation. Detoxification of AFB1 by A. vasica extract was further confirmed by liquid chromatography–mass spectrometry (LC–MS) analysis. Phytochemical analysis revealed the presence of alkaloids in methanolic extract of A. vasica leaves. A partially purified alkaloid from A. vasica leaves by preparative TLC exhibited strong AFB1 detoxification activity.</description><subject>Adhatoda vasica</subject><subject>Aflatoxin B1</subject><subject>Aflatoxin B1 - antagonists & inhibitors</subject><subject>Alkaloids - isolation & purification</subject><subject>Alkaloids - metabolism</subject><subject>Chromatography, Liquid</subject><subject>Chromatography, Thin Layer</subject><subject>Detoxification</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Justicia - chemistry</subject><subject>Kinetics</subject><subject>Mass Spectrometry</subject><subject>Medicinal plant</subject><subject>Plant Extracts - isolation & purification</subject><subject>Plant Extracts - metabolism</subject><subject>Plant Leaves - chemistry</subject><subject>Temperature</subject><subject>Time Factors</subject><issn>0944-5013</issn><issn>1618-0623</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE2LFDEQhoMo7rj6D0Ry9NJtVZJOpy_Cun7CohcFbyGTVDBDf6xJz7D7700zq0dPBVXvW_XWw9hLhBYB9ZtDOyWfqbQCULbQtwDmEduhRtOAFvIx28GgVNPV8QV7VsoBANVgxFN2IeQgjDSwYz_f07rcpZi8W9My8yVyF0e39Wb-Dvn-nruZu99HWo6F092anV95zMvER3InKpvjKvyqjuD4yZW6iH8lKs_Zk-jGQi8e6iX78fHD9-vPzc23T1-ur24ar3q1NmbohJd77AckI3RUoHvv4t65gEr0XgvU4JXSAY1RknoRuoEUoEHqQjTykr0-773NS01ZVjul4mkc3bxFttiBkELLbqhSdZb6vJSSKdrbnCaX7y2C3Zjagz0ztRtTC72tTKvt1cOF436i8M_0F2IVvD0LqP55SpRt8YlmTyFl8qsNS_r_hT-fW4jD</recordid><startdate>201404</startdate><enddate>201404</enddate><creator>Vijayanandraj, S.</creator><creator>Brinda, R.</creator><creator>Kannan, K.</creator><creator>Adhithya, R.</creator><creator>Vinothini, S.</creator><creator>Senthil, K.</creator><creator>Chinta, Ramakoteswara Rao</creator><creator>Paranidharan, V.</creator><creator>Velazhahan, R.</creator><general>Elsevier GmbH</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201404</creationdate><title>Detoxification of aflatoxin B1 by an aqueous extract from leaves of Adhatoda vasica Nees</title><author>Vijayanandraj, S. ; Brinda, R. ; Kannan, K. ; Adhithya, R. ; Vinothini, S. ; Senthil, K. ; Chinta, Ramakoteswara Rao ; Paranidharan, V. ; Velazhahan, R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c474t-8952c3b1791e826f4067cafbaad1427c62160c446d18843e72d59e40181e5df83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Adhatoda vasica</topic><topic>Aflatoxin B1</topic><topic>Aflatoxin B1 - antagonists & inhibitors</topic><topic>Alkaloids - isolation & purification</topic><topic>Alkaloids - metabolism</topic><topic>Chromatography, Liquid</topic><topic>Chromatography, Thin Layer</topic><topic>Detoxification</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Justicia - chemistry</topic><topic>Kinetics</topic><topic>Mass Spectrometry</topic><topic>Medicinal plant</topic><topic>Plant Extracts - isolation & purification</topic><topic>Plant Extracts - metabolism</topic><topic>Plant Leaves - chemistry</topic><topic>Temperature</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vijayanandraj, S.</creatorcontrib><creatorcontrib>Brinda, R.</creatorcontrib><creatorcontrib>Kannan, K.</creatorcontrib><creatorcontrib>Adhithya, R.</creatorcontrib><creatorcontrib>Vinothini, S.</creatorcontrib><creatorcontrib>Senthil, K.</creatorcontrib><creatorcontrib>Chinta, Ramakoteswara Rao</creatorcontrib><creatorcontrib>Paranidharan, V.</creatorcontrib><creatorcontrib>Velazhahan, R.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Microbiological research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vijayanandraj, S.</au><au>Brinda, R.</au><au>Kannan, K.</au><au>Adhithya, R.</au><au>Vinothini, S.</au><au>Senthil, K.</au><au>Chinta, Ramakoteswara Rao</au><au>Paranidharan, V.</au><au>Velazhahan, R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detoxification of aflatoxin B1 by an aqueous extract from leaves of Adhatoda vasica Nees</atitle><jtitle>Microbiological research</jtitle><addtitle>Microbiol Res</addtitle><date>2014-04</date><risdate>2014</risdate><volume>169</volume><issue>4</issue><spage>294</spage><epage>300</epage><pages>294-300</pages><issn>0944-5013</issn><eissn>1618-0623</eissn><abstract>The effectiveness of aqueous extracts of various medicinal plants in detoxification of aflatoxin B1 (AFB1) was tested in vitro by thin-layer chromatography and enzyme-linked immunosorbent assay (ELISA). Among the different plant extracts, the leaf extract of Vasaka (Adhatoda vasica Nees) showed the maximum degradation of AFB1 (≥98%) after incubation for 24h at 37°C. The aflatoxin detoxifying activity of the A. vasica leaf extract was significantly reduced by heating to 100°C for 10min or autoclaving at 121°C for 20min. Dialysis had no effect on aflatoxin detoxifying ability of A. vasica extract and the dialyzed extract showed similar level of detoxification of AFB1 as that of the untreated extract. A time course study of aflatoxin detoxification by A. vasica extract showed that 69% of the toxin was degraded within 6h and ≥95% degradation was observed after 24h of incubation. Detoxification of AFB1 by A. vasica extract was further confirmed by liquid chromatography–mass spectrometry (LC–MS) analysis. Phytochemical analysis revealed the presence of alkaloids in methanolic extract of A. vasica leaves. 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| subjects | Adhatoda vasica Aflatoxin B1 Aflatoxin B1 - antagonists & inhibitors Alkaloids - isolation & purification Alkaloids - metabolism Chromatography, Liquid Chromatography, Thin Layer Detoxification Enzyme-Linked Immunosorbent Assay Justicia - chemistry Kinetics Mass Spectrometry Medicinal plant Plant Extracts - isolation & purification Plant Extracts - metabolism Plant Leaves - chemistry Temperature Time Factors |
| title | Detoxification of aflatoxin B1 by an aqueous extract from leaves of Adhatoda vasica Nees |
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