Nuclear localization of huntingtin during spermatogenesis
Huntingtin is a ubiquitous cytoplasmic protein. Mutant huntingtin causes Huntington’s disease and its intranuclear inclusion is associated with cytotoxicity. Nuclear localization of normal huntingtin is detected in the oocyte up to 2.5 days post coitum. Therefore, huntingtin is expected to reside in...
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| Veröffentlicht in: | Neurological sciences 2014-03, Vol.35 (3), p.459-462 |
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| creator | Im, Wooseok Chung, Jinyoung Lee, Soon-Tae Chu, Kon Kim, Min-Wook Kim, Manho |
| description | Huntingtin is a ubiquitous cytoplasmic protein. Mutant huntingtin causes Huntington’s disease and its intranuclear inclusion is associated with cytotoxicity. Nuclear localization of normal huntingtin is detected in the oocyte up to 2.5 days post coitum. Therefore, huntingtin is expected to reside in the nucleus even before fertilization. The present study determined normal huntingtin distribution during spermatogenesis. Testicles from an adult male Sprague–Dawley rat were stained with anti-huntingtin antibody and nuclear counterstaining was performed with 4′,6-diamidino-2-phenylindole. Concerning nuclear localization, huntingtin was detected in the spermatids, whereas predominant cytoplasmic localization of it was evident in the spermatogonia. Between the primary and secondary spermatocytes, huntingtin appeared to be delocalized in the nuclei when meiosis occurred. The findings provide evidence that normal huntingtin is transported to the nuclear compartment during the meiotic stage of spermatogenesis. |
| doi_str_mv | 10.1007/s10072-013-1515-5 |
| format | Article |
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Mutant huntingtin causes Huntington’s disease and its intranuclear inclusion is associated with cytotoxicity. Nuclear localization of normal huntingtin is detected in the oocyte up to 2.5 days post coitum. Therefore, huntingtin is expected to reside in the nucleus even before fertilization. The present study determined normal huntingtin distribution during spermatogenesis. Testicles from an adult male Sprague–Dawley rat were stained with anti-huntingtin antibody and nuclear counterstaining was performed with 4′,6-diamidino-2-phenylindole. Concerning nuclear localization, huntingtin was detected in the spermatids, whereas predominant cytoplasmic localization of it was evident in the spermatogonia. Between the primary and secondary spermatocytes, huntingtin appeared to be delocalized in the nuclei when meiosis occurred. The findings provide evidence that normal huntingtin is transported to the nuclear compartment during the meiotic stage of spermatogenesis.</description><identifier>ISSN: 1590-1874</identifier><identifier>EISSN: 1590-3478</identifier><identifier>DOI: 10.1007/s10072-013-1515-5</identifier><identifier>PMID: 23955097</identifier><language>eng</language><publisher>Milan: Springer Milan</publisher><subject>Animals ; Cell Nucleus - metabolism ; Huntingtin Protein ; Male ; Medicine ; Medicine & Public Health ; Nerve Tissue Proteins - metabolism ; Neurology ; Neuroradiology ; Neurosciences ; Neurosurgery ; Nuclear Proteins - metabolism ; Psychiatry ; Rats ; Rats, Sprague-Dawley ; Short Communication ; Spermatids - ultrastructure ; Spermatogenesis - physiology ; Testis - metabolism</subject><ispartof>Neurological sciences, 2014-03, Vol.35 (3), p.459-462</ispartof><rights>Springer-Verlag Italia 2013</rights><rights>Springer-Verlag Italia 2014</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c372t-ee55699435e025d313225ec89213b6263d42282d7681e5a03f2da77c7d78bab33</citedby><cites>FETCH-LOGICAL-c372t-ee55699435e025d313225ec89213b6263d42282d7681e5a03f2da77c7d78bab33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10072-013-1515-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10072-013-1515-5$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23955097$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Im, Wooseok</creatorcontrib><creatorcontrib>Chung, Jinyoung</creatorcontrib><creatorcontrib>Lee, Soon-Tae</creatorcontrib><creatorcontrib>Chu, Kon</creatorcontrib><creatorcontrib>Kim, Min-Wook</creatorcontrib><creatorcontrib>Kim, Manho</creatorcontrib><title>Nuclear localization of huntingtin during spermatogenesis</title><title>Neurological sciences</title><addtitle>Neurol Sci</addtitle><addtitle>Neurol Sci</addtitle><description>Huntingtin is a ubiquitous cytoplasmic protein. Mutant huntingtin causes Huntington’s disease and its intranuclear inclusion is associated with cytotoxicity. Nuclear localization of normal huntingtin is detected in the oocyte up to 2.5 days post coitum. Therefore, huntingtin is expected to reside in the nucleus even before fertilization. The present study determined normal huntingtin distribution during spermatogenesis. Testicles from an adult male Sprague–Dawley rat were stained with anti-huntingtin antibody and nuclear counterstaining was performed with 4′,6-diamidino-2-phenylindole. Concerning nuclear localization, huntingtin was detected in the spermatids, whereas predominant cytoplasmic localization of it was evident in the spermatogonia. Between the primary and secondary spermatocytes, huntingtin appeared to be delocalized in the nuclei when meiosis occurred. The findings provide evidence that normal huntingtin is transported to the nuclear compartment during the meiotic stage of spermatogenesis.</description><subject>Animals</subject><subject>Cell Nucleus - metabolism</subject><subject>Huntingtin Protein</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Nerve Tissue Proteins - metabolism</subject><subject>Neurology</subject><subject>Neuroradiology</subject><subject>Neurosciences</subject><subject>Neurosurgery</subject><subject>Nuclear Proteins - metabolism</subject><subject>Psychiatry</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Short Communication</subject><subject>Spermatids - ultrastructure</subject><subject>Spermatogenesis - physiology</subject><subject>Testis - metabolism</subject><issn>1590-1874</issn><issn>1590-3478</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kE1LxDAQhoMo7rr6A7xIwYuXaiZpmuYoi18getFzSNvp2qVtatIe9Neb0lVE8JDJHJ55Z3gIOQV6CZTKKz9VFlPgMQgQsdgjSxCKxjyR2f6uh0wmC3Lk_ZZSCgnwQ7JgXAlBlVwS9TQWDRoXNbYwTf1phtp2ka2it7Eb6m4TXlSOLnSR79G1ZrAb7NDX_pgcVKbxeLL7V-T19uZlfR8_Pt89rK8f44JLNsSIQqRKJVwgZaLkwBkTWGSKAc9TlvIyYSxjpUwzQGEor1hppCxkKbPc5JyvyMWc2zv7PqIfdFv7ApvGdGhHr0FQyDgHRgN6_gfd2tF14bqJoooqJmSgYKYKZ713WOne1a1xHxqonozq2asOXvXkVYswc7ZLHvMWy5-Jb5EBYDPg-0kWul-r_039Al05gNU</recordid><startdate>20140301</startdate><enddate>20140301</enddate><creator>Im, Wooseok</creator><creator>Chung, Jinyoung</creator><creator>Lee, Soon-Tae</creator><creator>Chu, Kon</creator><creator>Kim, Min-Wook</creator><creator>Kim, Manho</creator><general>Springer Milan</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88G</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>K9-</scope><scope>K9.</scope><scope>M0R</scope><scope>M0S</scope><scope>M1P</scope><scope>M2M</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PSYQQ</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20140301</creationdate><title>Nuclear localization of huntingtin during spermatogenesis</title><author>Im, Wooseok ; Chung, Jinyoung ; Lee, Soon-Tae ; Chu, Kon ; Kim, Min-Wook ; Kim, Manho</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c372t-ee55699435e025d313225ec89213b6263d42282d7681e5a03f2da77c7d78bab33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Cell Nucleus - metabolism</topic><topic>Huntingtin Protein</topic><topic>Male</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Nerve Tissue Proteins - metabolism</topic><topic>Neurology</topic><topic>Neuroradiology</topic><topic>Neurosciences</topic><topic>Neurosurgery</topic><topic>Nuclear Proteins - metabolism</topic><topic>Psychiatry</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Short Communication</topic><topic>Spermatids - ultrastructure</topic><topic>Spermatogenesis - physiology</topic><topic>Testis - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Im, Wooseok</creatorcontrib><creatorcontrib>Chung, Jinyoung</creatorcontrib><creatorcontrib>Lee, Soon-Tae</creatorcontrib><creatorcontrib>Chu, Kon</creatorcontrib><creatorcontrib>Kim, Min-Wook</creatorcontrib><creatorcontrib>Kim, Manho</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Psychology Database (Alumni)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Consumer Health Database (Alumni Edition)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Consumer Health Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Psychology Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest One Psychology</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Neurological sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Im, Wooseok</au><au>Chung, Jinyoung</au><au>Lee, Soon-Tae</au><au>Chu, Kon</au><au>Kim, Min-Wook</au><au>Kim, Manho</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nuclear localization of huntingtin during spermatogenesis</atitle><jtitle>Neurological sciences</jtitle><stitle>Neurol Sci</stitle><addtitle>Neurol Sci</addtitle><date>2014-03-01</date><risdate>2014</risdate><volume>35</volume><issue>3</issue><spage>459</spage><epage>462</epage><pages>459-462</pages><issn>1590-1874</issn><eissn>1590-3478</eissn><abstract>Huntingtin is a ubiquitous cytoplasmic protein. Mutant huntingtin causes Huntington’s disease and its intranuclear inclusion is associated with cytotoxicity. Nuclear localization of normal huntingtin is detected in the oocyte up to 2.5 days post coitum. Therefore, huntingtin is expected to reside in the nucleus even before fertilization. The present study determined normal huntingtin distribution during spermatogenesis. Testicles from an adult male Sprague–Dawley rat were stained with anti-huntingtin antibody and nuclear counterstaining was performed with 4′,6-diamidino-2-phenylindole. Concerning nuclear localization, huntingtin was detected in the spermatids, whereas predominant cytoplasmic localization of it was evident in the spermatogonia. Between the primary and secondary spermatocytes, huntingtin appeared to be delocalized in the nuclei when meiosis occurred. 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| ispartof | Neurological sciences, 2014-03, Vol.35 (3), p.459-462 |
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| subjects | Animals Cell Nucleus - metabolism Huntingtin Protein Male Medicine Medicine & Public Health Nerve Tissue Proteins - metabolism Neurology Neuroradiology Neurosciences Neurosurgery Nuclear Proteins - metabolism Psychiatry Rats Rats, Sprague-Dawley Short Communication Spermatids - ultrastructure Spermatogenesis - physiology Testis - metabolism |
| title | Nuclear localization of huntingtin during spermatogenesis |
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