Right atrium cholinergic deficit in septic rats
Abstract Heart rate variability (HRV) is mainly determined by the influence of both branches of the Autonomic Nervous System over the sinus node. Low HRV has been associated with a worse prognosis in patients with sepsis. The objective of this study was to explain the reduction in HRV during experim...
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| Veröffentlicht in: | Autonomic neuroscience 2014-02, Vol.180, p.17-23 |
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| creator | Contreras, Paola Migliaro, Eduardo R Suhr, Bruno |
| description | Abstract Heart rate variability (HRV) is mainly determined by the influence of both branches of the Autonomic Nervous System over the sinus node. Low HRV has been associated with a worse prognosis in patients with sepsis. The objective of this study was to explain the reduction in HRV during experimental sepsis in adult rats. We recorded the heart's electrical activity by telemetry in conscious unrestrained male rats before and 1 day after the induction of peritonitis ( N = 39) or sham peritonitis ( N = 15). Then, we analyzed the chronotropic responsiveness of the isolated heart to the autonomic neurotransmitters and determined catecholamine concentrations in blood plasma and acetylcholine and choline concentrations in the right atrium. The surviving septic rats ( N = 33) had increased heart rate (HR) and diminished HRV. Despite the higher HR in situ, the spontaneous basal HR in septic and sham isolated hearts was the same. The isolated septic hearts showed acetylcholine hypersensitivity (log (IC50 ,M) = − 7.2 ± 0.2 vs. − 6.0 ± 0.4, P = 0.025) and lower concentrations of choline in their right atriums (in nMol/mg protein: 0.6 ± 0.1 vs. 1.6 ± 0.6, P = 0.013). Norepinephrine concentration in blood plasma from septic rats was higher (in ng/ml: 29.2 ± 8.4 vs. 5.8 ± 4.1, P = 0.019). In conclusion, septic rats present a deregulation of the autonomic nervous system, not only sympathetic overexcitation but also parasympathetic dysfunction. |
| doi_str_mv | 10.1016/j.autneu.2013.10.002 |
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Low HRV has been associated with a worse prognosis in patients with sepsis. The objective of this study was to explain the reduction in HRV during experimental sepsis in adult rats. We recorded the heart's electrical activity by telemetry in conscious unrestrained male rats before and 1 day after the induction of peritonitis ( N = 39) or sham peritonitis ( N = 15). Then, we analyzed the chronotropic responsiveness of the isolated heart to the autonomic neurotransmitters and determined catecholamine concentrations in blood plasma and acetylcholine and choline concentrations in the right atrium. The surviving septic rats ( N = 33) had increased heart rate (HR) and diminished HRV. Despite the higher HR in situ, the spontaneous basal HR in septic and sham isolated hearts was the same. The isolated septic hearts showed acetylcholine hypersensitivity (log (IC50 ,M) = − 7.2 ± 0.2 vs. − 6.0 ± 0.4, P = 0.025) and lower concentrations of choline in their right atriums (in nMol/mg protein: 0.6 ± 0.1 vs. 1.6 ± 0.6, P = 0.013). Norepinephrine concentration in blood plasma from septic rats was higher (in ng/ml: 29.2 ± 8.4 vs. 5.8 ± 4.1, P = 0.019). In conclusion, septic rats present a deregulation of the autonomic nervous system, not only sympathetic overexcitation but also parasympathetic dysfunction.</description><identifier>ISSN: 1566-0702</identifier><identifier>EISSN: 1872-7484</identifier><identifier>DOI: 10.1016/j.autneu.2013.10.002</identifier><identifier>PMID: 24269487</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Acetylcholine ; Acetylcholine - pharmacology ; Advanced Basic Science ; Animals ; Atropine - pharmacology ; Autonomic dysfunction ; Choline - analysis ; Cholinergic Fibers - physiology ; Dose-Response Relationship, Drug ; Heart - drug effects ; Heart Atria - chemistry ; Heart Atria - physiopathology ; Heart Conduction System - drug effects ; Heart Conduction System - physiopathology ; Heart Rate - physiology ; Heart rate variability ; Male ; Medical Education ; Neurotransmitter Agents - blood ; Norepinephrine - blood ; Norepinephrine - pharmacology ; Parasympathetic Nervous System - physiopathology ; Peritonitis - physiopathology ; Random Allocation ; Rats ; Rats, Wistar ; Sepsis ; Sepsis - physiopathology ; Sympathetic Nervous System - physiopathology ; Telemetry</subject><ispartof>Autonomic neuroscience, 2014-02, Vol.180, p.17-23</ispartof><rights>Elsevier B.V.</rights><rights>2013 Elsevier B.V.</rights><rights>2013.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c450t-885082c0092ce04b5a42461f5796919d36b06fd2b06af36da9cf1a630f6b77fe3</citedby><cites>FETCH-LOGICAL-c450t-885082c0092ce04b5a42461f5796919d36b06fd2b06af36da9cf1a630f6b77fe3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.autneu.2013.10.002$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,27911,27912,45982</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24269487$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Contreras, Paola</creatorcontrib><creatorcontrib>Migliaro, Eduardo R</creatorcontrib><creatorcontrib>Suhr, Bruno</creatorcontrib><title>Right atrium cholinergic deficit in septic rats</title><title>Autonomic neuroscience</title><addtitle>Auton Neurosci</addtitle><description>Abstract Heart rate variability (HRV) is mainly determined by the influence of both branches of the Autonomic Nervous System over the sinus node. Low HRV has been associated with a worse prognosis in patients with sepsis. The objective of this study was to explain the reduction in HRV during experimental sepsis in adult rats. We recorded the heart's electrical activity by telemetry in conscious unrestrained male rats before and 1 day after the induction of peritonitis ( N = 39) or sham peritonitis ( N = 15). Then, we analyzed the chronotropic responsiveness of the isolated heart to the autonomic neurotransmitters and determined catecholamine concentrations in blood plasma and acetylcholine and choline concentrations in the right atrium. The surviving septic rats ( N = 33) had increased heart rate (HR) and diminished HRV. Despite the higher HR in situ, the spontaneous basal HR in septic and sham isolated hearts was the same. The isolated septic hearts showed acetylcholine hypersensitivity (log (IC50 ,M) = − 7.2 ± 0.2 vs. − 6.0 ± 0.4, P = 0.025) and lower concentrations of choline in their right atriums (in nMol/mg protein: 0.6 ± 0.1 vs. 1.6 ± 0.6, P = 0.013). Norepinephrine concentration in blood plasma from septic rats was higher (in ng/ml: 29.2 ± 8.4 vs. 5.8 ± 4.1, P = 0.019). In conclusion, septic rats present a deregulation of the autonomic nervous system, not only sympathetic overexcitation but also parasympathetic dysfunction.</description><subject>Acetylcholine</subject><subject>Acetylcholine - pharmacology</subject><subject>Advanced Basic Science</subject><subject>Animals</subject><subject>Atropine - pharmacology</subject><subject>Autonomic dysfunction</subject><subject>Choline - analysis</subject><subject>Cholinergic Fibers - physiology</subject><subject>Dose-Response Relationship, Drug</subject><subject>Heart - drug effects</subject><subject>Heart Atria - chemistry</subject><subject>Heart Atria - physiopathology</subject><subject>Heart Conduction System - drug effects</subject><subject>Heart Conduction System - physiopathology</subject><subject>Heart Rate - physiology</subject><subject>Heart rate variability</subject><subject>Male</subject><subject>Medical Education</subject><subject>Neurotransmitter Agents - blood</subject><subject>Norepinephrine - blood</subject><subject>Norepinephrine - pharmacology</subject><subject>Parasympathetic Nervous System - physiopathology</subject><subject>Peritonitis - physiopathology</subject><subject>Random Allocation</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Sepsis</subject><subject>Sepsis - physiopathology</subject><subject>Sympathetic Nervous System - physiopathology</subject><subject>Telemetry</subject><issn>1566-0702</issn><issn>1872-7484</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1r3DAQhkVJaD7af1CCj7l4d_RhyboUypK0gUAgac9CK48Sbb32VpIL-feR2U0PvexlZnh5Zwael5AvFBYUqFxuFnbKA04LBpQXaQHAPpBz2ipWK9GKkzI3UtaggJ2Ri5Q2ANCClh_JGRNMatGqc7J8DM8vubI5hmlbuZexDwPG5-CqDn1wIVdhqBLuclGizekTOfW2T_j50C_Jr9ubn6sf9f3D97vVt_vaiQZy3bYNtMwBaOYQxLqxgglJfaO01FR3XK5B-o6Vaj2XndXOUys5eLlWyiO_JNf7u7s4_pkwZbMNyWHf2wHHKRnaACjdMKqPW4UGJZSgUKxib3VxTCmiN7sYtja-Ggpmpmo2Zk_VzFRntVAta1eHD9N6i92_pXeMxfB1b8CC5G_AaJILODjsQkSXTTeGYx_-P-BKEMHZ_je-YtqMUxwKbkNNYgbM05zsHCzlBQMHzt8AwsedBg</recordid><startdate>20140201</startdate><enddate>20140201</enddate><creator>Contreras, Paola</creator><creator>Migliaro, Eduardo R</creator><creator>Suhr, Bruno</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TK</scope></search><sort><creationdate>20140201</creationdate><title>Right atrium cholinergic deficit in septic rats</title><author>Contreras, Paola ; Migliaro, Eduardo R ; Suhr, Bruno</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c450t-885082c0092ce04b5a42461f5796919d36b06fd2b06af36da9cf1a630f6b77fe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Acetylcholine</topic><topic>Acetylcholine - pharmacology</topic><topic>Advanced Basic Science</topic><topic>Animals</topic><topic>Atropine - pharmacology</topic><topic>Autonomic dysfunction</topic><topic>Choline - analysis</topic><topic>Cholinergic Fibers - physiology</topic><topic>Dose-Response Relationship, Drug</topic><topic>Heart - drug effects</topic><topic>Heart Atria - chemistry</topic><topic>Heart Atria - physiopathology</topic><topic>Heart Conduction System - drug effects</topic><topic>Heart Conduction System - physiopathology</topic><topic>Heart Rate - physiology</topic><topic>Heart rate variability</topic><topic>Male</topic><topic>Medical Education</topic><topic>Neurotransmitter Agents - blood</topic><topic>Norepinephrine - blood</topic><topic>Norepinephrine - pharmacology</topic><topic>Parasympathetic Nervous System - physiopathology</topic><topic>Peritonitis - physiopathology</topic><topic>Random Allocation</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Sepsis</topic><topic>Sepsis - physiopathology</topic><topic>Sympathetic Nervous System - physiopathology</topic><topic>Telemetry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Contreras, Paola</creatorcontrib><creatorcontrib>Migliaro, Eduardo R</creatorcontrib><creatorcontrib>Suhr, Bruno</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Neurosciences Abstracts</collection><jtitle>Autonomic neuroscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Contreras, Paola</au><au>Migliaro, Eduardo R</au><au>Suhr, Bruno</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Right atrium cholinergic deficit in septic rats</atitle><jtitle>Autonomic neuroscience</jtitle><addtitle>Auton Neurosci</addtitle><date>2014-02-01</date><risdate>2014</risdate><volume>180</volume><spage>17</spage><epage>23</epage><pages>17-23</pages><issn>1566-0702</issn><eissn>1872-7484</eissn><abstract>Abstract Heart rate variability (HRV) is mainly determined by the influence of both branches of the Autonomic Nervous System over the sinus node. Low HRV has been associated with a worse prognosis in patients with sepsis. The objective of this study was to explain the reduction in HRV during experimental sepsis in adult rats. We recorded the heart's electrical activity by telemetry in conscious unrestrained male rats before and 1 day after the induction of peritonitis ( N = 39) or sham peritonitis ( N = 15). Then, we analyzed the chronotropic responsiveness of the isolated heart to the autonomic neurotransmitters and determined catecholamine concentrations in blood plasma and acetylcholine and choline concentrations in the right atrium. The surviving septic rats ( N = 33) had increased heart rate (HR) and diminished HRV. Despite the higher HR in situ, the spontaneous basal HR in septic and sham isolated hearts was the same. The isolated septic hearts showed acetylcholine hypersensitivity (log (IC50 ,M) = − 7.2 ± 0.2 vs. − 6.0 ± 0.4, P = 0.025) and lower concentrations of choline in their right atriums (in nMol/mg protein: 0.6 ± 0.1 vs. 1.6 ± 0.6, P = 0.013). Norepinephrine concentration in blood plasma from septic rats was higher (in ng/ml: 29.2 ± 8.4 vs. 5.8 ± 4.1, P = 0.019). In conclusion, septic rats present a deregulation of the autonomic nervous system, not only sympathetic overexcitation but also parasympathetic dysfunction.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>24269487</pmid><doi>10.1016/j.autneu.2013.10.002</doi><tpages>7</tpages></addata></record> |
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| subjects | Acetylcholine Acetylcholine - pharmacology Advanced Basic Science Animals Atropine - pharmacology Autonomic dysfunction Choline - analysis Cholinergic Fibers - physiology Dose-Response Relationship, Drug Heart - drug effects Heart Atria - chemistry Heart Atria - physiopathology Heart Conduction System - drug effects Heart Conduction System - physiopathology Heart Rate - physiology Heart rate variability Male Medical Education Neurotransmitter Agents - blood Norepinephrine - blood Norepinephrine - pharmacology Parasympathetic Nervous System - physiopathology Peritonitis - physiopathology Random Allocation Rats Rats, Wistar Sepsis Sepsis - physiopathology Sympathetic Nervous System - physiopathology Telemetry |
| title | Right atrium cholinergic deficit in septic rats |
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