Pelvic axis-based gait analysis for ataxic mice
•Unlike the conventional method in which the nasal apex and base of the tail are used for mouse gait analysis, we have developed pelvic axis.•Based on this axis, we have established pelvic axis-based gait analysis method.•The scatter plots together with the limb angle analyses clearly show the diffe...
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| Veröffentlicht in: | Journal of neuroscience methods 2013-09, Vol.219 (1), p.162-168 |
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| creator | Takayanagi, Naoki Beppu, Hidehiko Mizutani, Kenmei Tomita, Yutaka Nagao, Shizuko Suzuki, Shoichi Orand, Abbas Takahashi, Hisahide Sonoda, Shigeru |
| description | •Unlike the conventional method in which the nasal apex and base of the tail are used for mouse gait analysis, we have developed pelvic axis.•Based on this axis, we have established pelvic axis-based gait analysis method.•The scatter plots together with the limb angle analyses clearly show the differentiability of the mice groups with central nervous system degeneration and the healthy mice group.
Although different gait analysis methods such as Walking Track Analysis exist, they cannot be used to demonstrate the physical condition of mice with specific gait disorder characteristic. Therefore, we developed a new method for the gait analysis of such mice to accurately assess hind limb angle based on the pelvic axis.
We established and verified a gait analysis method capable of pelvic axis-based limb angle measurement by video-recording the gait of a control mice group (C57BL/6J(B6)) and three ataxic mice (ataxic B6-wob/t, Parkinson's disease model (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine treated (MPTP)), and cerebellum hypoplasia (cytosine-β-d-arabinofuranoside treated)) from the ventral side.
The assessed hind limb angles of B6-wob/t and MPTP-treated mice were significantly wider than B6 mice (p |
| doi_str_mv | 10.1016/j.jneumeth.2013.07.011 |
| format | Article |
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Although different gait analysis methods such as Walking Track Analysis exist, they cannot be used to demonstrate the physical condition of mice with specific gait disorder characteristic. Therefore, we developed a new method for the gait analysis of such mice to accurately assess hind limb angle based on the pelvic axis.
We established and verified a gait analysis method capable of pelvic axis-based limb angle measurement by video-recording the gait of a control mice group (C57BL/6J(B6)) and three ataxic mice (ataxic B6-wob/t, Parkinson's disease model (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine treated (MPTP)), and cerebellum hypoplasia (cytosine-β-d-arabinofuranoside treated)) from the ventral side.
The assessed hind limb angles of B6-wob/t and MPTP-treated mice were significantly wider than B6 mice (p<0.01). Moreover, we could draw separating lines with slopes of minus one that could separate the data of each group in the scatter plot of the normalized hind limb step width and angle.
We found no significance when we applied the already existing nose–tail method for the analysis of the hind limb angles of B6 and B6-wob/t mice. In the nose–tail method, since the whole body axis of the trunk varies while the trunk of the mouse is laterally bent changing the hind limb angle, B6 and B6-wob/t mice could not be differentiated. However, the two mice groups could be differentiated by the pelvic axis-based gait analysis method.
The pelvic axis-based gait analysis method is promising and valid for mice with gait disorder.</description><identifier>ISSN: 0165-0270</identifier><identifier>EISSN: 1872-678X</identifier><identifier>DOI: 10.1016/j.jneumeth.2013.07.011</identifier><identifier>PMID: 23912024</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine - pharmacology ; Animals ; Antimetabolites, Antineoplastic - pharmacology ; Ataxia ; Ataxia - physiopathology ; B6-wob/t mice ; Biomechanical Phenomena ; Cytarabine - pharmacology ; Diet ; Dopamine Agents - pharmacology ; Gait - physiology ; Gait analysis ; Hind limb angle ; Hindlimb - anatomy & histology ; Hindlimb - physiology ; Mice ; Mice, Inbred C57BL ; Mice, Neurologic Mutants ; Pelvis - physiology</subject><ispartof>Journal of neuroscience methods, 2013-09, Vol.219 (1), p.162-168</ispartof><rights>2013 Elsevier B.V.</rights><rights>Copyright © 2013 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c551t-c7712c80e340c2c9543e55e2340cb288ffd73523e33bdf9bb68462c2f00a6dd93</citedby><cites>FETCH-LOGICAL-c551t-c7712c80e340c2c9543e55e2340cb288ffd73523e33bdf9bb68462c2f00a6dd93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0165027013002495$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23912024$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Takayanagi, Naoki</creatorcontrib><creatorcontrib>Beppu, Hidehiko</creatorcontrib><creatorcontrib>Mizutani, Kenmei</creatorcontrib><creatorcontrib>Tomita, Yutaka</creatorcontrib><creatorcontrib>Nagao, Shizuko</creatorcontrib><creatorcontrib>Suzuki, Shoichi</creatorcontrib><creatorcontrib>Orand, Abbas</creatorcontrib><creatorcontrib>Takahashi, Hisahide</creatorcontrib><creatorcontrib>Sonoda, Shigeru</creatorcontrib><title>Pelvic axis-based gait analysis for ataxic mice</title><title>Journal of neuroscience methods</title><addtitle>J Neurosci Methods</addtitle><description>•Unlike the conventional method in which the nasal apex and base of the tail are used for mouse gait analysis, we have developed pelvic axis.•Based on this axis, we have established pelvic axis-based gait analysis method.•The scatter plots together with the limb angle analyses clearly show the differentiability of the mice groups with central nervous system degeneration and the healthy mice group.
Although different gait analysis methods such as Walking Track Analysis exist, they cannot be used to demonstrate the physical condition of mice with specific gait disorder characteristic. Therefore, we developed a new method for the gait analysis of such mice to accurately assess hind limb angle based on the pelvic axis.
We established and verified a gait analysis method capable of pelvic axis-based limb angle measurement by video-recording the gait of a control mice group (C57BL/6J(B6)) and three ataxic mice (ataxic B6-wob/t, Parkinson's disease model (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine treated (MPTP)), and cerebellum hypoplasia (cytosine-β-d-arabinofuranoside treated)) from the ventral side.
The assessed hind limb angles of B6-wob/t and MPTP-treated mice were significantly wider than B6 mice (p<0.01). Moreover, we could draw separating lines with slopes of minus one that could separate the data of each group in the scatter plot of the normalized hind limb step width and angle.
We found no significance when we applied the already existing nose–tail method for the analysis of the hind limb angles of B6 and B6-wob/t mice. In the nose–tail method, since the whole body axis of the trunk varies while the trunk of the mouse is laterally bent changing the hind limb angle, B6 and B6-wob/t mice could not be differentiated. However, the two mice groups could be differentiated by the pelvic axis-based gait analysis method.
The pelvic axis-based gait analysis method is promising and valid for mice with gait disorder.</description><subject>1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine - pharmacology</subject><subject>Animals</subject><subject>Antimetabolites, Antineoplastic - pharmacology</subject><subject>Ataxia</subject><subject>Ataxia - physiopathology</subject><subject>B6-wob/t mice</subject><subject>Biomechanical Phenomena</subject><subject>Cytarabine - pharmacology</subject><subject>Diet</subject><subject>Dopamine Agents - pharmacology</subject><subject>Gait - physiology</subject><subject>Gait analysis</subject><subject>Hind limb angle</subject><subject>Hindlimb - anatomy & histology</subject><subject>Hindlimb - physiology</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Neurologic Mutants</subject><subject>Pelvis - physiology</subject><issn>0165-0270</issn><issn>1872-678X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtOwzAQRS0EoqXwC1WWbJKO7SROdqCKl1QJFiCxsxx7Ao7yKHZS0b8nVVu2XY1Gc-5c6RAypxBRoOmiiqoWhwb774gB5RGICCg9I1OaCRamIvs8J9MRTEJgAibkyvsKAOIc0ksyYTynDFg8JYs3rDdWB-rX-rBQHk3wpWwfqFbVW299UHYuUP141kFjNV6Ti1LVHm8Oc0Y-Hh_el8_h6vXpZXm_CnWS0D7UQlCmM0Aeg2Y6T2KOSYJstxYsy8rSCJ4wjpwXpsyLIs3ilGlWAqjUmJzPyO3-79p1PwP6XjbWa6xr1WI3eEkTAJFzGsNpNGZ5yjMaixFN96h2nfcOS7l2tlFuKynInVdZyaNXufMqQcjR6xicHzqGokHzHzuKHIG7PYCjlI1FJ7222Go01qHupensqY4_19qKrA</recordid><startdate>20130930</startdate><enddate>20130930</enddate><creator>Takayanagi, Naoki</creator><creator>Beppu, Hidehiko</creator><creator>Mizutani, Kenmei</creator><creator>Tomita, Yutaka</creator><creator>Nagao, Shizuko</creator><creator>Suzuki, Shoichi</creator><creator>Orand, Abbas</creator><creator>Takahashi, Hisahide</creator><creator>Sonoda, Shigeru</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TK</scope></search><sort><creationdate>20130930</creationdate><title>Pelvic axis-based gait analysis for ataxic mice</title><author>Takayanagi, Naoki ; Beppu, Hidehiko ; Mizutani, Kenmei ; Tomita, Yutaka ; Nagao, Shizuko ; Suzuki, Shoichi ; Orand, Abbas ; Takahashi, Hisahide ; Sonoda, Shigeru</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c551t-c7712c80e340c2c9543e55e2340cb288ffd73523e33bdf9bb68462c2f00a6dd93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine - pharmacology</topic><topic>Animals</topic><topic>Antimetabolites, Antineoplastic - pharmacology</topic><topic>Ataxia</topic><topic>Ataxia - physiopathology</topic><topic>B6-wob/t mice</topic><topic>Biomechanical Phenomena</topic><topic>Cytarabine - pharmacology</topic><topic>Diet</topic><topic>Dopamine Agents - pharmacology</topic><topic>Gait - physiology</topic><topic>Gait analysis</topic><topic>Hind limb angle</topic><topic>Hindlimb - anatomy & histology</topic><topic>Hindlimb - physiology</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Neurologic Mutants</topic><topic>Pelvis - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Takayanagi, Naoki</creatorcontrib><creatorcontrib>Beppu, Hidehiko</creatorcontrib><creatorcontrib>Mizutani, Kenmei</creatorcontrib><creatorcontrib>Tomita, Yutaka</creatorcontrib><creatorcontrib>Nagao, Shizuko</creatorcontrib><creatorcontrib>Suzuki, Shoichi</creatorcontrib><creatorcontrib>Orand, Abbas</creatorcontrib><creatorcontrib>Takahashi, Hisahide</creatorcontrib><creatorcontrib>Sonoda, Shigeru</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Neurosciences Abstracts</collection><jtitle>Journal of neuroscience methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Takayanagi, Naoki</au><au>Beppu, Hidehiko</au><au>Mizutani, Kenmei</au><au>Tomita, Yutaka</au><au>Nagao, Shizuko</au><au>Suzuki, Shoichi</au><au>Orand, Abbas</au><au>Takahashi, Hisahide</au><au>Sonoda, Shigeru</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pelvic axis-based gait analysis for ataxic mice</atitle><jtitle>Journal of neuroscience methods</jtitle><addtitle>J Neurosci Methods</addtitle><date>2013-09-30</date><risdate>2013</risdate><volume>219</volume><issue>1</issue><spage>162</spage><epage>168</epage><pages>162-168</pages><issn>0165-0270</issn><eissn>1872-678X</eissn><abstract>•Unlike the conventional method in which the nasal apex and base of the tail are used for mouse gait analysis, we have developed pelvic axis.•Based on this axis, we have established pelvic axis-based gait analysis method.•The scatter plots together with the limb angle analyses clearly show the differentiability of the mice groups with central nervous system degeneration and the healthy mice group.
Although different gait analysis methods such as Walking Track Analysis exist, they cannot be used to demonstrate the physical condition of mice with specific gait disorder characteristic. Therefore, we developed a new method for the gait analysis of such mice to accurately assess hind limb angle based on the pelvic axis.
We established and verified a gait analysis method capable of pelvic axis-based limb angle measurement by video-recording the gait of a control mice group (C57BL/6J(B6)) and three ataxic mice (ataxic B6-wob/t, Parkinson's disease model (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine treated (MPTP)), and cerebellum hypoplasia (cytosine-β-d-arabinofuranoside treated)) from the ventral side.
The assessed hind limb angles of B6-wob/t and MPTP-treated mice were significantly wider than B6 mice (p<0.01). Moreover, we could draw separating lines with slopes of minus one that could separate the data of each group in the scatter plot of the normalized hind limb step width and angle.
We found no significance when we applied the already existing nose–tail method for the analysis of the hind limb angles of B6 and B6-wob/t mice. In the nose–tail method, since the whole body axis of the trunk varies while the trunk of the mouse is laterally bent changing the hind limb angle, B6 and B6-wob/t mice could not be differentiated. However, the two mice groups could be differentiated by the pelvic axis-based gait analysis method.
The pelvic axis-based gait analysis method is promising and valid for mice with gait disorder.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>23912024</pmid><doi>10.1016/j.jneumeth.2013.07.011</doi><tpages>7</tpages></addata></record> |
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| ispartof | Journal of neuroscience methods, 2013-09, Vol.219 (1), p.162-168 |
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| subjects | 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine - pharmacology Animals Antimetabolites, Antineoplastic - pharmacology Ataxia Ataxia - physiopathology B6-wob/t mice Biomechanical Phenomena Cytarabine - pharmacology Diet Dopamine Agents - pharmacology Gait - physiology Gait analysis Hind limb angle Hindlimb - anatomy & histology Hindlimb - physiology Mice Mice, Inbred C57BL Mice, Neurologic Mutants Pelvis - physiology |
| title | Pelvic axis-based gait analysis for ataxic mice |
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