Effect of phenylpyrrole-resistance on fitness parameters and ochratoxin production in Aspergillus carbonarius
The risk of resistance development to fludioxonil and the potential implications of resistance mutations to ochratoxin production in Aspergillus carbonarius were investigated. Mutants of A. carbonarius highly resistant to phenylpyrroles were isolated at a high mutation frequency after N-MNTG-mutagen...
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| Veröffentlicht in: | International journal of food microbiology 2013-08, Vol.165 (3), p.287-294 |
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| creator | Malandrakis, Anastasios A. Vattis, Konstantinos N. Doukas, Eleftherios G. Markoglou, Anastasios N. |
| description | The risk of resistance development to fludioxonil and the potential implications of resistance mutations to ochratoxin production in Aspergillus carbonarius were investigated. Mutants of A. carbonarius highly resistant to phenylpyrroles were isolated at a high mutation frequency after N-MNTG-mutagenesis and selection on media containing fludioxonil. A highly reduced sensitivity to fungicides belonging to the same cross-resistance group (AHDs and phenylpyrroles) such as the aromatic hydrocarbon tolclofos-methyl and the dicarboximide fungicides iprodione and vinclozolin was also observed. No cross-resistance relationships were found between fludioxonil and the triazole epoxiconazole, the anilinopyrimidine cyprodinil and the chloronitrile chlorothalonil. Interestingly, fludioxonil-resistant isolates were highly sensitive to the QoI fungicide pyraclostrobin compared to the wild-type parental strain. Fitness studies revealed that resistance mutation(s) had a negative effect on mycelial growth, resistance to osmotic stress and pathogenicity of the fludioxonil-resistant strains. Mycotoxin analysis showed that most fludioxonil-resistant strains produce less quantities of ochratoxin A (OTA) than the wild-type strain both when grown on artificial medium and on grapes. Increased osmotic sensitivity and reduced pathogenicity of the mutant strains were significantly correlated with reduced ochratoxin production in vivo but not in vitro. The above-mentioned data indicate that fludioxonil is an excellent fungicide for the control of A. carbonarius in grapes and a valuable asset for farmers in terms of resistance management and ochratoxin contamination of grapes, vine products and wines.
•Fludioxonil is highly effective against A. carbonarius.•Highly phenylpyrrole-resistant laboratory isolates were readily isolated.•Laboratory mutants carried fitness penalties and produced less OTA on grapes.•Pyraclostrobin was highly effective against fludioxonil-resistant isolates. |
| doi_str_mv | 10.1016/j.ijfoodmicro.2013.05.019 |
| format | Article |
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•Fludioxonil is highly effective against A. carbonarius.•Highly phenylpyrrole-resistant laboratory isolates were readily isolated.•Laboratory mutants carried fitness penalties and produced less OTA on grapes.•Pyraclostrobin was highly effective against fludioxonil-resistant isolates.</description><identifier>ISSN: 0168-1605</identifier><identifier>EISSN: 1879-3460</identifier><identifier>DOI: 10.1016/j.ijfoodmicro.2013.05.019</identifier><identifier>PMID: 23800740</identifier><identifier>CODEN: IJFMDD</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Aromatic hydrocarbons ; Aspergillus - drug effects ; Aspergillus - genetics ; Aspergillus - growth & development ; Aspergillus - physiology ; Aspergillus carbonarius ; Biological and medical sciences ; Dioxoles - pharmacology ; Drug Resistance, Fungal - physiology ; Fludioxonil ; Food industries ; Food microbiology ; Food toxicology ; Fundamental and applied biological sciences. Psychology ; Fungicide resistance ; Fungicides, Industrial - pharmacology ; HPLC ; Mutation ; Ochratoxins - biosynthesis ; Osmotic stress ; OTA ; OTB ; Pyrroles - pharmacology ; Vitaceae ; Vitis - microbiology</subject><ispartof>International journal of food microbiology, 2013-08, Vol.165 (3), p.287-294</ispartof><rights>2013 Elsevier B.V.</rights><rights>2014 INIST-CNRS</rights><rights>Copyright © 2013 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c473t-a2d50f0dac31745e604050c02f112c6e0525327aa7634be14a19e625bd02f7b93</citedby><cites>FETCH-LOGICAL-c473t-a2d50f0dac31745e604050c02f112c6e0525327aa7634be14a19e625bd02f7b93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ijfoodmicro.2013.05.019$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=27627879$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23800740$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Malandrakis, Anastasios A.</creatorcontrib><creatorcontrib>Vattis, Konstantinos N.</creatorcontrib><creatorcontrib>Doukas, Eleftherios G.</creatorcontrib><creatorcontrib>Markoglou, Anastasios N.</creatorcontrib><title>Effect of phenylpyrrole-resistance on fitness parameters and ochratoxin production in Aspergillus carbonarius</title><title>International journal of food microbiology</title><addtitle>Int J Food Microbiol</addtitle><description>The risk of resistance development to fludioxonil and the potential implications of resistance mutations to ochratoxin production in Aspergillus carbonarius were investigated. Mutants of A. carbonarius highly resistant to phenylpyrroles were isolated at a high mutation frequency after N-MNTG-mutagenesis and selection on media containing fludioxonil. A highly reduced sensitivity to fungicides belonging to the same cross-resistance group (AHDs and phenylpyrroles) such as the aromatic hydrocarbon tolclofos-methyl and the dicarboximide fungicides iprodione and vinclozolin was also observed. No cross-resistance relationships were found between fludioxonil and the triazole epoxiconazole, the anilinopyrimidine cyprodinil and the chloronitrile chlorothalonil. Interestingly, fludioxonil-resistant isolates were highly sensitive to the QoI fungicide pyraclostrobin compared to the wild-type parental strain. Fitness studies revealed that resistance mutation(s) had a negative effect on mycelial growth, resistance to osmotic stress and pathogenicity of the fludioxonil-resistant strains. Mycotoxin analysis showed that most fludioxonil-resistant strains produce less quantities of ochratoxin A (OTA) than the wild-type strain both when grown on artificial medium and on grapes. Increased osmotic sensitivity and reduced pathogenicity of the mutant strains were significantly correlated with reduced ochratoxin production in vivo but not in vitro. The above-mentioned data indicate that fludioxonil is an excellent fungicide for the control of A. carbonarius in grapes and a valuable asset for farmers in terms of resistance management and ochratoxin contamination of grapes, vine products and wines.
•Fludioxonil is highly effective against A. carbonarius.•Highly phenylpyrrole-resistant laboratory isolates were readily isolated.•Laboratory mutants carried fitness penalties and produced less OTA on grapes.•Pyraclostrobin was highly effective against fludioxonil-resistant isolates.</description><subject>Aromatic hydrocarbons</subject><subject>Aspergillus - drug effects</subject><subject>Aspergillus - genetics</subject><subject>Aspergillus - growth & development</subject><subject>Aspergillus - physiology</subject><subject>Aspergillus carbonarius</subject><subject>Biological and medical sciences</subject><subject>Dioxoles - pharmacology</subject><subject>Drug Resistance, Fungal - physiology</subject><subject>Fludioxonil</subject><subject>Food industries</subject><subject>Food microbiology</subject><subject>Food toxicology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungicide resistance</subject><subject>Fungicides, Industrial - pharmacology</subject><subject>HPLC</subject><subject>Mutation</subject><subject>Ochratoxins - biosynthesis</subject><subject>Osmotic stress</subject><subject>OTA</subject><subject>OTB</subject><subject>Pyrroles - pharmacology</subject><subject>Vitaceae</subject><subject>Vitis - microbiology</subject><issn>0168-1605</issn><issn>1879-3460</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkcuO1DAQRS0EYpqBX0BhgcQmofyKO8tRa3hII7GBteU4ZcatJA6uBNF_j0fdPHawsko6Va66h7FXHBoOvH17bOIxpDRM0efUCOCyAd0A7x6xHd-brpaqhcdsV9h9zVvQV-wZ0REAtJTwlF0JuQcwCnZsug0B_VqlUC33OJ_G5ZRzGrHOSJFWN3us0lyFuM5IVC0uuwlXzFS5eaiSv89uTT_iXC05DZtfY4FLdUML5q9xHDeqvMt9ml2OGz1nT4IbCV9c3mv25d3t58OH-u7T-4-Hm7vaKyPX2olBQ4DBecmN0tiCAg0eROBc-BZBCy2Fcc60UvXIleMdtkL3Q0FM38lr9uY8t2z1bUNa7RTJ4zi6GdNGluty_h46qf6NKiWl6fZGFLQ7oyV1oozBLjlOLp8sB_sgxh7tX2LsgxgL2hYxpffl5Zutn3D43fnLRAFeXwBH3o0hl-gj_eFMK0xRW7jDmcOS3_eI2ZKPWDQNMReRdkjxP9b5CQlGs5U</recordid><startdate>20130801</startdate><enddate>20130801</enddate><creator>Malandrakis, Anastasios A.</creator><creator>Vattis, Konstantinos N.</creator><creator>Doukas, Eleftherios G.</creator><creator>Markoglou, Anastasios N.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>20130801</creationdate><title>Effect of phenylpyrrole-resistance on fitness parameters and ochratoxin production in Aspergillus carbonarius</title><author>Malandrakis, Anastasios A. ; Vattis, Konstantinos N. ; Doukas, Eleftherios G. ; Markoglou, Anastasios N.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c473t-a2d50f0dac31745e604050c02f112c6e0525327aa7634be14a19e625bd02f7b93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Aromatic hydrocarbons</topic><topic>Aspergillus - drug effects</topic><topic>Aspergillus - genetics</topic><topic>Aspergillus - growth & development</topic><topic>Aspergillus - physiology</topic><topic>Aspergillus carbonarius</topic><topic>Biological and medical sciences</topic><topic>Dioxoles - pharmacology</topic><topic>Drug Resistance, Fungal - physiology</topic><topic>Fludioxonil</topic><topic>Food industries</topic><topic>Food microbiology</topic><topic>Food toxicology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fungicide resistance</topic><topic>Fungicides, Industrial - pharmacology</topic><topic>HPLC</topic><topic>Mutation</topic><topic>Ochratoxins - biosynthesis</topic><topic>Osmotic stress</topic><topic>OTA</topic><topic>OTB</topic><topic>Pyrroles - pharmacology</topic><topic>Vitaceae</topic><topic>Vitis - microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Malandrakis, Anastasios A.</creatorcontrib><creatorcontrib>Vattis, Konstantinos N.</creatorcontrib><creatorcontrib>Doukas, Eleftherios G.</creatorcontrib><creatorcontrib>Markoglou, Anastasios N.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>International journal of food microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Malandrakis, Anastasios A.</au><au>Vattis, Konstantinos N.</au><au>Doukas, Eleftherios G.</au><au>Markoglou, Anastasios N.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of phenylpyrrole-resistance on fitness parameters and ochratoxin production in Aspergillus carbonarius</atitle><jtitle>International journal of food microbiology</jtitle><addtitle>Int J Food Microbiol</addtitle><date>2013-08-01</date><risdate>2013</risdate><volume>165</volume><issue>3</issue><spage>287</spage><epage>294</epage><pages>287-294</pages><issn>0168-1605</issn><eissn>1879-3460</eissn><coden>IJFMDD</coden><abstract>The risk of resistance development to fludioxonil and the potential implications of resistance mutations to ochratoxin production in Aspergillus carbonarius were investigated. Mutants of A. carbonarius highly resistant to phenylpyrroles were isolated at a high mutation frequency after N-MNTG-mutagenesis and selection on media containing fludioxonil. A highly reduced sensitivity to fungicides belonging to the same cross-resistance group (AHDs and phenylpyrroles) such as the aromatic hydrocarbon tolclofos-methyl and the dicarboximide fungicides iprodione and vinclozolin was also observed. No cross-resistance relationships were found between fludioxonil and the triazole epoxiconazole, the anilinopyrimidine cyprodinil and the chloronitrile chlorothalonil. Interestingly, fludioxonil-resistant isolates were highly sensitive to the QoI fungicide pyraclostrobin compared to the wild-type parental strain. Fitness studies revealed that resistance mutation(s) had a negative effect on mycelial growth, resistance to osmotic stress and pathogenicity of the fludioxonil-resistant strains. Mycotoxin analysis showed that most fludioxonil-resistant strains produce less quantities of ochratoxin A (OTA) than the wild-type strain both when grown on artificial medium and on grapes. Increased osmotic sensitivity and reduced pathogenicity of the mutant strains were significantly correlated with reduced ochratoxin production in vivo but not in vitro. The above-mentioned data indicate that fludioxonil is an excellent fungicide for the control of A. carbonarius in grapes and a valuable asset for farmers in terms of resistance management and ochratoxin contamination of grapes, vine products and wines.
•Fludioxonil is highly effective against A. carbonarius.•Highly phenylpyrrole-resistant laboratory isolates were readily isolated.•Laboratory mutants carried fitness penalties and produced less OTA on grapes.•Pyraclostrobin was highly effective against fludioxonil-resistant isolates.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>23800740</pmid><doi>10.1016/j.ijfoodmicro.2013.05.019</doi><tpages>8</tpages></addata></record> |
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| subjects | Aromatic hydrocarbons Aspergillus - drug effects Aspergillus - genetics Aspergillus - growth & development Aspergillus - physiology Aspergillus carbonarius Biological and medical sciences Dioxoles - pharmacology Drug Resistance, Fungal - physiology Fludioxonil Food industries Food microbiology Food toxicology Fundamental and applied biological sciences. Psychology Fungicide resistance Fungicides, Industrial - pharmacology HPLC Mutation Ochratoxins - biosynthesis Osmotic stress OTA OTB Pyrroles - pharmacology Vitaceae Vitis - microbiology |
| title | Effect of phenylpyrrole-resistance on fitness parameters and ochratoxin production in Aspergillus carbonarius |
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