Protein Production in Yarrowia lipolytica Via Fusion to the Secreted Lipase Lip2p

We established a strategy for protein production and purification via expression in Yarrowia lipolytica as Lip2p fusion protein. To evaluate the expression system a cysteine-rich miniprotein, an antibody fragment and an enzyme showing galactose oxidase activity were chosen. These proteins have varyi...

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Veröffentlicht in:	Molecular biotechnology 2014, Vol.56 (1), p.79-90
Hauptverfasser:	Hofmeyer, Thomas, Bulani, Siyavuya Ishmael, Grzeschik, Julius, Krah, Simon, Glotzbach, Bernhard, Uth, Christina, Avrutina, Olga, Brecht, Michael, Göringer, Hans Ulrich, van Zyl, Petrus, Kolmar, Harald
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibodies Biochemistry Biological Techniques Biotechnology Cell Biology Chemistry Chemistry and Materials Science Cloning, Molecular Contaminants Cysteine - metabolism Enzymes Fungal Proteins - genetics Fungal Proteins - metabolism galactose Gene Expression Genetic Vectors Human Genetics Lipase - genetics Lipase - metabolism monitoring Protein Science Protein Structure, Secondary proteinases Proteins proteolysis Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism Recombinant Fusion Proteins - secretion Yarrowia - enzymology Yarrowia - genetics Yarrowia lipolytica
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