Distribution of dihydrofolate reductase (dhfr) and dihydropteroate synthase (dhps) mutant alleles in Plasmodium vivax isolates from Thailand
The prevalent distribution of dihydrofolate reductase (pvdhfr) and Plasmodium vivax dihydropteroate synthase (pvdhps) single nucleotide polymorphisms in P. vivax isolates from Thailand. •We studied the genetic polymorphisms in pvdhfr and pvdhps gene.•This study was carried out in malaria endemic are...
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| Veröffentlicht in: | Acta tropica 2013-10, Vol.128 (1), p.137-143 |
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| creator | Thongdee, Pimwan Kuesap, Jiraporn Rungsihirunrat, Kanchana Tippawangkosol, Pongsri Mungthin, Mathirut Na-Bangchang, Kesara |
| description | The prevalent distribution of dihydrofolate reductase (pvdhfr) and Plasmodium vivax dihydropteroate synthase (pvdhps) single nucleotide polymorphisms in P. vivax isolates from Thailand.
•We studied the genetic polymorphisms in pvdhfr and pvdhps gene.•This study was carried out in malaria endemic areas of Thailand along the Thai-Myanmar, Thai-Cambodian, and Thai-Malaysian borders.•We examined SNP-haplotypes of the pvdhfr at positions 13, 33, 57, 58, 61, 117 and 173.•We examined SNP-haplotypes of the pvdhps at positions 383 and 553.•P. vivax isolates in Thailand carried mutant combination alleles of pvdhfr and pvdhps.
The analysis of prevalence and distribution of pvdhfr and pvdhps mutations were performed in 169 samples collected from patients with Plasmodium vivax infection who attended the malaria clinics in the provinces along the three international borders of Thailand (Thai-Myanmar, Thai-Cambodian, and Thai-Malaysian borders). SNP-haplotypes of the pvdhfr at amino acid positions 13, 33, 57, 58, 61, 117, and 173 and of the pvdhps at positions 383 and 553 were examined by nested PCR-RFLP. Significant differences in the prevalence and distribution of pvdhfr and pvdhps combination alleles were observed in P. vivax isolates collected from all the three border areas. The most prevalent combination alleles were triple mutant pvdhfr 57L/58R/117T alleles/double wild-type pvdhps alleles (n=18), double mutant pvdhfr 58R/117N alleles/double wild-type pvdhps alleles (n=10), and triple mutant pvdhfr 58R/61M/117N alleles/double wild-type pvdhps alleles (n=52) or with single mutant pvdhps 383G allele (n=28), respectively. These information on prevalence and patterns of pvdhfr and pvdhps polymorphisms obtained from the present study suggest the presence of SP pressure on P. vivax isolates in Thailand which could be linked to the introduction of malaria from neighboring countries. Results did not support the application of SP for P. vivax control program in Thailand as well as the neighboring countries. |
| doi_str_mv | 10.1016/j.actatropica.2013.07.005 |
| format | Article |
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•We studied the genetic polymorphisms in pvdhfr and pvdhps gene.•This study was carried out in malaria endemic areas of Thailand along the Thai-Myanmar, Thai-Cambodian, and Thai-Malaysian borders.•We examined SNP-haplotypes of the pvdhfr at positions 13, 33, 57, 58, 61, 117 and 173.•We examined SNP-haplotypes of the pvdhps at positions 383 and 553.•P. vivax isolates in Thailand carried mutant combination alleles of pvdhfr and pvdhps.
The analysis of prevalence and distribution of pvdhfr and pvdhps mutations were performed in 169 samples collected from patients with Plasmodium vivax infection who attended the malaria clinics in the provinces along the three international borders of Thailand (Thai-Myanmar, Thai-Cambodian, and Thai-Malaysian borders). SNP-haplotypes of the pvdhfr at amino acid positions 13, 33, 57, 58, 61, 117, and 173 and of the pvdhps at positions 383 and 553 were examined by nested PCR-RFLP. Significant differences in the prevalence and distribution of pvdhfr and pvdhps combination alleles were observed in P. vivax isolates collected from all the three border areas. The most prevalent combination alleles were triple mutant pvdhfr 57L/58R/117T alleles/double wild-type pvdhps alleles (n=18), double mutant pvdhfr 58R/117N alleles/double wild-type pvdhps alleles (n=10), and triple mutant pvdhfr 58R/61M/117N alleles/double wild-type pvdhps alleles (n=52) or with single mutant pvdhps 383G allele (n=28), respectively. These information on prevalence and patterns of pvdhfr and pvdhps polymorphisms obtained from the present study suggest the presence of SP pressure on P. vivax isolates in Thailand which could be linked to the introduction of malaria from neighboring countries. Results did not support the application of SP for P. vivax control program in Thailand as well as the neighboring countries.</description><identifier>ISSN: 0001-706X</identifier><identifier>EISSN: 1873-6254</identifier><identifier>DOI: 10.1016/j.actatropica.2013.07.005</identifier><identifier>PMID: 23880285</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Adolescent ; Adult ; Aged ; Aged, 80 and over ; alleles ; amino acids ; Child ; dihydrofolate reductase ; Dihydropteroate Synthase - genetics ; Female ; Gene Frequency ; Genetic Variation ; Haplotypes ; Humans ; Malaria ; Malaria, Vivax - parasitology ; Male ; Middle Aged ; mutants ; patients ; PCR-RFLP ; Plasmodium vivax ; Plasmodium vivax - enzymology ; Plasmodium vivax - genetics ; Plasmodium vivax - isolation & purification ; Plasmodium vivax dihydrofolate reductase (pvdhfr) ; Plasmodium vivax dihydropteroate synthase (pvdhps) ; Polymerase Chain Reaction ; Polymorphism, Restriction Fragment Length ; Polymorphism, Single Nucleotide ; restriction fragment length polymorphism ; Sulfadoxine-pyrimethamine (SP) ; Tetrahydrofolate Dehydrogenase - genetics ; Thailand ; Young Adult</subject><ispartof>Acta tropica, 2013-10, Vol.128 (1), p.137-143</ispartof><rights>2013 Elsevier B.V.</rights><rights>Copyright © 2013 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c434t-c700e544e9ef98e97764adc5cf9099d679ceca0ae610790ffad814ba812755b3</citedby><cites>FETCH-LOGICAL-c434t-c700e544e9ef98e97764adc5cf9099d679ceca0ae610790ffad814ba812755b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0001706X1300185X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23880285$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Thongdee, Pimwan</creatorcontrib><creatorcontrib>Kuesap, Jiraporn</creatorcontrib><creatorcontrib>Rungsihirunrat, Kanchana</creatorcontrib><creatorcontrib>Tippawangkosol, Pongsri</creatorcontrib><creatorcontrib>Mungthin, Mathirut</creatorcontrib><creatorcontrib>Na-Bangchang, Kesara</creatorcontrib><title>Distribution of dihydrofolate reductase (dhfr) and dihydropteroate synthase (dhps) mutant alleles in Plasmodium vivax isolates from Thailand</title><title>Acta tropica</title><addtitle>Acta Trop</addtitle><description>The prevalent distribution of dihydrofolate reductase (pvdhfr) and Plasmodium vivax dihydropteroate synthase (pvdhps) single nucleotide polymorphisms in P. vivax isolates from Thailand.
•We studied the genetic polymorphisms in pvdhfr and pvdhps gene.•This study was carried out in malaria endemic areas of Thailand along the Thai-Myanmar, Thai-Cambodian, and Thai-Malaysian borders.•We examined SNP-haplotypes of the pvdhfr at positions 13, 33, 57, 58, 61, 117 and 173.•We examined SNP-haplotypes of the pvdhps at positions 383 and 553.•P. vivax isolates in Thailand carried mutant combination alleles of pvdhfr and pvdhps.
The analysis of prevalence and distribution of pvdhfr and pvdhps mutations were performed in 169 samples collected from patients with Plasmodium vivax infection who attended the malaria clinics in the provinces along the three international borders of Thailand (Thai-Myanmar, Thai-Cambodian, and Thai-Malaysian borders). SNP-haplotypes of the pvdhfr at amino acid positions 13, 33, 57, 58, 61, 117, and 173 and of the pvdhps at positions 383 and 553 were examined by nested PCR-RFLP. Significant differences in the prevalence and distribution of pvdhfr and pvdhps combination alleles were observed in P. vivax isolates collected from all the three border areas. The most prevalent combination alleles were triple mutant pvdhfr 57L/58R/117T alleles/double wild-type pvdhps alleles (n=18), double mutant pvdhfr 58R/117N alleles/double wild-type pvdhps alleles (n=10), and triple mutant pvdhfr 58R/61M/117N alleles/double wild-type pvdhps alleles (n=52) or with single mutant pvdhps 383G allele (n=28), respectively. These information on prevalence and patterns of pvdhfr and pvdhps polymorphisms obtained from the present study suggest the presence of SP pressure on P. vivax isolates in Thailand which could be linked to the introduction of malaria from neighboring countries. Results did not support the application of SP for P. vivax control program in Thailand as well as the neighboring countries.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>alleles</subject><subject>amino acids</subject><subject>Child</subject><subject>dihydrofolate reductase</subject><subject>Dihydropteroate Synthase - genetics</subject><subject>Female</subject><subject>Gene Frequency</subject><subject>Genetic Variation</subject><subject>Haplotypes</subject><subject>Humans</subject><subject>Malaria</subject><subject>Malaria, Vivax - parasitology</subject><subject>Male</subject><subject>Middle Aged</subject><subject>mutants</subject><subject>patients</subject><subject>PCR-RFLP</subject><subject>Plasmodium vivax</subject><subject>Plasmodium vivax - enzymology</subject><subject>Plasmodium vivax - genetics</subject><subject>Plasmodium vivax - isolation & purification</subject><subject>Plasmodium vivax dihydrofolate reductase (pvdhfr)</subject><subject>Plasmodium vivax dihydropteroate synthase (pvdhps)</subject><subject>Polymerase Chain Reaction</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>Polymorphism, Single Nucleotide</subject><subject>restriction fragment length polymorphism</subject><subject>Sulfadoxine-pyrimethamine (SP)</subject><subject>Tetrahydrofolate Dehydrogenase - genetics</subject><subject>Thailand</subject><subject>Young Adult</subject><issn>0001-706X</issn><issn>1873-6254</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc2O0zAURi0EYsrAK4DZzSwSbn4dL1GHAaSRQKJI7CzXvqaukjjYTkXfgYceh3YQO1hZls797mcfQl4XkBdQtG_2uVRRRu8mq2ReQlHlwHKA5hFZFR2rsrZs6sdkBQBFxqD9dkGehbBPt5I15VNyUVZdB2XXrMivGxuit9s5WjdSZ6i2u6P2zrheRqQe9ZxWBaRXemf8NZWjfkCmiN4tUDiOcXdmpnBNhznKMVLZ99hjoHakn3sZBqftPNCDPcif1Ibf-YEa7wa62Unbp-Tn5ImRfcAX5_OSbG7fbdYfsrtP7z-u395lqq7qmCkGgE1dI0fDO-SMtbXUqlGGA-e6ZVyhkiCxLYBxMEbqrqi3slue32yrS3J1ip28-zFjiGKwQWGfKqCbgygaAMYSW_8brauqbFtoeEL5CVXeheDRiMnbQfqjKEAs2sRe_KVNLNoEMJG0pdmX5zXzdkD9Z_LBUwJenQAjnZDfvQ3i65eUkJpC17Buabo-EZg-7mDRi6Asjgq19aii0M7-R5F7Fq27ZA</recordid><startdate>20131001</startdate><enddate>20131001</enddate><creator>Thongdee, Pimwan</creator><creator>Kuesap, Jiraporn</creator><creator>Rungsihirunrat, Kanchana</creator><creator>Tippawangkosol, Pongsri</creator><creator>Mungthin, Mathirut</creator><creator>Na-Bangchang, Kesara</creator><general>Elsevier B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>C1K</scope><scope>F1W</scope><scope>H95</scope><scope>H97</scope><scope>L.G</scope><scope>M7N</scope></search><sort><creationdate>20131001</creationdate><title>Distribution of dihydrofolate reductase (dhfr) and dihydropteroate synthase (dhps) mutant alleles in Plasmodium vivax isolates from Thailand</title><author>Thongdee, Pimwan ; Kuesap, Jiraporn ; Rungsihirunrat, Kanchana ; Tippawangkosol, Pongsri ; Mungthin, Mathirut ; Na-Bangchang, Kesara</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c434t-c700e544e9ef98e97764adc5cf9099d679ceca0ae610790ffad814ba812755b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>alleles</topic><topic>amino acids</topic><topic>Child</topic><topic>dihydrofolate reductase</topic><topic>Dihydropteroate Synthase - genetics</topic><topic>Female</topic><topic>Gene Frequency</topic><topic>Genetic Variation</topic><topic>Haplotypes</topic><topic>Humans</topic><topic>Malaria</topic><topic>Malaria, Vivax - parasitology</topic><topic>Male</topic><topic>Middle Aged</topic><topic>mutants</topic><topic>patients</topic><topic>PCR-RFLP</topic><topic>Plasmodium vivax</topic><topic>Plasmodium vivax - enzymology</topic><topic>Plasmodium vivax - genetics</topic><topic>Plasmodium vivax - isolation & purification</topic><topic>Plasmodium vivax dihydrofolate reductase (pvdhfr)</topic><topic>Plasmodium vivax dihydropteroate synthase (pvdhps)</topic><topic>Polymerase Chain Reaction</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>Polymorphism, Single Nucleotide</topic><topic>restriction fragment length polymorphism</topic><topic>Sulfadoxine-pyrimethamine (SP)</topic><topic>Tetrahydrofolate Dehydrogenase - genetics</topic><topic>Thailand</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Thongdee, Pimwan</creatorcontrib><creatorcontrib>Kuesap, Jiraporn</creatorcontrib><creatorcontrib>Rungsihirunrat, Kanchana</creatorcontrib><creatorcontrib>Tippawangkosol, Pongsri</creatorcontrib><creatorcontrib>Mungthin, Mathirut</creatorcontrib><creatorcontrib>Na-Bangchang, Kesara</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Acta tropica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Thongdee, Pimwan</au><au>Kuesap, Jiraporn</au><au>Rungsihirunrat, Kanchana</au><au>Tippawangkosol, Pongsri</au><au>Mungthin, Mathirut</au><au>Na-Bangchang, Kesara</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Distribution of dihydrofolate reductase (dhfr) and dihydropteroate synthase (dhps) mutant alleles in Plasmodium vivax isolates from Thailand</atitle><jtitle>Acta tropica</jtitle><addtitle>Acta Trop</addtitle><date>2013-10-01</date><risdate>2013</risdate><volume>128</volume><issue>1</issue><spage>137</spage><epage>143</epage><pages>137-143</pages><issn>0001-706X</issn><eissn>1873-6254</eissn><abstract>The prevalent distribution of dihydrofolate reductase (pvdhfr) and Plasmodium vivax dihydropteroate synthase (pvdhps) single nucleotide polymorphisms in P. vivax isolates from Thailand.
•We studied the genetic polymorphisms in pvdhfr and pvdhps gene.•This study was carried out in malaria endemic areas of Thailand along the Thai-Myanmar, Thai-Cambodian, and Thai-Malaysian borders.•We examined SNP-haplotypes of the pvdhfr at positions 13, 33, 57, 58, 61, 117 and 173.•We examined SNP-haplotypes of the pvdhps at positions 383 and 553.•P. vivax isolates in Thailand carried mutant combination alleles of pvdhfr and pvdhps.
The analysis of prevalence and distribution of pvdhfr and pvdhps mutations were performed in 169 samples collected from patients with Plasmodium vivax infection who attended the malaria clinics in the provinces along the three international borders of Thailand (Thai-Myanmar, Thai-Cambodian, and Thai-Malaysian borders). SNP-haplotypes of the pvdhfr at amino acid positions 13, 33, 57, 58, 61, 117, and 173 and of the pvdhps at positions 383 and 553 were examined by nested PCR-RFLP. Significant differences in the prevalence and distribution of pvdhfr and pvdhps combination alleles were observed in P. vivax isolates collected from all the three border areas. The most prevalent combination alleles were triple mutant pvdhfr 57L/58R/117T alleles/double wild-type pvdhps alleles (n=18), double mutant pvdhfr 58R/117N alleles/double wild-type pvdhps alleles (n=10), and triple mutant pvdhfr 58R/61M/117N alleles/double wild-type pvdhps alleles (n=52) or with single mutant pvdhps 383G allele (n=28), respectively. These information on prevalence and patterns of pvdhfr and pvdhps polymorphisms obtained from the present study suggest the presence of SP pressure on P. vivax isolates in Thailand which could be linked to the introduction of malaria from neighboring countries. Results did not support the application of SP for P. vivax control program in Thailand as well as the neighboring countries.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>23880285</pmid><doi>10.1016/j.actatropica.2013.07.005</doi><tpages>7</tpages></addata></record> |
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| ispartof | Acta tropica, 2013-10, Vol.128 (1), p.137-143 |
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| subjects | Adolescent Adult Aged Aged, 80 and over alleles amino acids Child dihydrofolate reductase Dihydropteroate Synthase - genetics Female Gene Frequency Genetic Variation Haplotypes Humans Malaria Malaria, Vivax - parasitology Male Middle Aged mutants patients PCR-RFLP Plasmodium vivax Plasmodium vivax - enzymology Plasmodium vivax - genetics Plasmodium vivax - isolation & purification Plasmodium vivax dihydrofolate reductase (pvdhfr) Plasmodium vivax dihydropteroate synthase (pvdhps) Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Polymorphism, Single Nucleotide restriction fragment length polymorphism Sulfadoxine-pyrimethamine (SP) Tetrahydrofolate Dehydrogenase - genetics Thailand Young Adult |
| title | Distribution of dihydrofolate reductase (dhfr) and dihydropteroate synthase (dhps) mutant alleles in Plasmodium vivax isolates from Thailand |
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