Succinate production by metabolically engineered Escherichia coli using sugarcane bagasse hydrolysate as the carbon source
► Enhancement of ATP supply increased succinate production in Escherichia coli BA204. ► Engineered E. coli was constructed for sugarcane bagasse hydrolysate utilization. ► Fermentation of sugarcane bagasse hydrolysate to produce succinate was studied. Efficient biosynthesis of succinate from a renew...
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| Veröffentlicht in: | Bioresource technology 2013-05, Vol.135, p.574-577 |
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| creator | Liu, Rongming Liang, Liya Cao, Weijia Wu, Mingke Chen, Kequan Ma, Jiangfeng Jiang, Min Wei, Ping Ouyang, Pingkai |
| description | ► Enhancement of ATP supply increased succinate production in Escherichia coli BA204. ► Engineered E. coli was constructed for sugarcane bagasse hydrolysate utilization. ► Fermentation of sugarcane bagasse hydrolysate to produce succinate was studied.
Efficient biosynthesis of succinate from a renewable biomass resource by engineered Escherichia coli is reported in this paper. Fermentation of sugarcane bagasse hydrolysate by engineered E. coli BA204, a pflB, ldhA, and ppc deletion strain overexpressing the ATP-forming phosphoenolpyruvate carboxykinase from Bacillus subtilis 168, produced a final succinate concentration of 15.85gL−1 with a high yield of 0.89gL−1 total sugar under anaerobic conditions. During dual-phase fermentations, initial aerobic growth facilitated subsequent anaerobic succinate production, with a final succinate concentration of 18.88gL−1 and a yield of 0.96gg−1 total sugar after 24h of anaerobic fermentation. The high succinate yield from sugarcane bagasse hydrolysate demonstrated a great potential application of renewable biomass as a feedstock for the economical production of succinate using metabolically engineered E. coli. |
| doi_str_mv | 10.1016/j.biortech.2012.08.120 |
| format | Article |
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Efficient biosynthesis of succinate from a renewable biomass resource by engineered Escherichia coli is reported in this paper. Fermentation of sugarcane bagasse hydrolysate by engineered E. coli BA204, a pflB, ldhA, and ppc deletion strain overexpressing the ATP-forming phosphoenolpyruvate carboxykinase from Bacillus subtilis 168, produced a final succinate concentration of 15.85gL−1 with a high yield of 0.89gL−1 total sugar under anaerobic conditions. During dual-phase fermentations, initial aerobic growth facilitated subsequent anaerobic succinate production, with a final succinate concentration of 18.88gL−1 and a yield of 0.96gg−1 total sugar after 24h of anaerobic fermentation. The high succinate yield from sugarcane bagasse hydrolysate demonstrated a great potential application of renewable biomass as a feedstock for the economical production of succinate using metabolically engineered E. coli.</description><identifier>ISSN: 0960-8524</identifier><identifier>EISSN: 1873-2976</identifier><identifier>DOI: 10.1016/j.biortech.2012.08.120</identifier><identifier>PMID: 23010211</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Anaerobiosis - drug effects ; ATP ; Bacillus subtilis ; Batch Cell Culture Techniques ; Bioreactors - microbiology ; Carbohydrates - pharmacology ; Carbon - pharmacology ; Cellulose - pharmacology ; Escherichia coli ; Escherichia coli - drug effects ; Escherichia coli - metabolism ; Escherichia coli BA204 ; Fermentation - drug effects ; Hydrolysis - drug effects ; Metabolic Engineering - methods ; Saccharum - chemistry ; Succinate ; Succinic Acid - metabolism ; Sugarcane bagasse hydrolysate</subject><ispartof>Bioresource technology, 2013-05, Vol.135, p.574-577</ispartof><rights>2012 Elsevier Ltd</rights><rights>Copyright © 2012 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c467t-79a60aa64db38275bbe6367f5bd3ea8407ec431030e94c7d2c79bcb95055c72f3</citedby><cites>FETCH-LOGICAL-c467t-79a60aa64db38275bbe6367f5bd3ea8407ec431030e94c7d2c79bcb95055c72f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0960852412013156$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23010211$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Rongming</creatorcontrib><creatorcontrib>Liang, Liya</creatorcontrib><creatorcontrib>Cao, Weijia</creatorcontrib><creatorcontrib>Wu, Mingke</creatorcontrib><creatorcontrib>Chen, Kequan</creatorcontrib><creatorcontrib>Ma, Jiangfeng</creatorcontrib><creatorcontrib>Jiang, Min</creatorcontrib><creatorcontrib>Wei, Ping</creatorcontrib><creatorcontrib>Ouyang, Pingkai</creatorcontrib><title>Succinate production by metabolically engineered Escherichia coli using sugarcane bagasse hydrolysate as the carbon source</title><title>Bioresource technology</title><addtitle>Bioresour Technol</addtitle><description>► Enhancement of ATP supply increased succinate production in Escherichia coli BA204. ► Engineered E. coli was constructed for sugarcane bagasse hydrolysate utilization. ► Fermentation of sugarcane bagasse hydrolysate to produce succinate was studied.
Efficient biosynthesis of succinate from a renewable biomass resource by engineered Escherichia coli is reported in this paper. Fermentation of sugarcane bagasse hydrolysate by engineered E. coli BA204, a pflB, ldhA, and ppc deletion strain overexpressing the ATP-forming phosphoenolpyruvate carboxykinase from Bacillus subtilis 168, produced a final succinate concentration of 15.85gL−1 with a high yield of 0.89gL−1 total sugar under anaerobic conditions. During dual-phase fermentations, initial aerobic growth facilitated subsequent anaerobic succinate production, with a final succinate concentration of 18.88gL−1 and a yield of 0.96gg−1 total sugar after 24h of anaerobic fermentation. The high succinate yield from sugarcane bagasse hydrolysate demonstrated a great potential application of renewable biomass as a feedstock for the economical production of succinate using metabolically engineered E. coli.</description><subject>Anaerobiosis - drug effects</subject><subject>ATP</subject><subject>Bacillus subtilis</subject><subject>Batch Cell Culture Techniques</subject><subject>Bioreactors - microbiology</subject><subject>Carbohydrates - pharmacology</subject><subject>Carbon - pharmacology</subject><subject>Cellulose - pharmacology</subject><subject>Escherichia coli</subject><subject>Escherichia coli - drug effects</subject><subject>Escherichia coli - metabolism</subject><subject>Escherichia coli BA204</subject><subject>Fermentation - drug effects</subject><subject>Hydrolysis - drug effects</subject><subject>Metabolic Engineering - methods</subject><subject>Saccharum - chemistry</subject><subject>Succinate</subject><subject>Succinic Acid - metabolism</subject><subject>Sugarcane bagasse hydrolysate</subject><issn>0960-8524</issn><issn>1873-2976</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhi0EokvhL1Q-ckkY27Gd3EBV-ZAqcQDOlj2Z3XiVTYqdIIVfj1fbcu1pLs-88_EwdiOgFiDMh2Md4pwWwqGWIGQNbS0kvGA70VpVyc6al2wHnYGq1bK5Ym9yPgKAEla-ZldSgQApxI79_bEixskvxB_S3K-4xHniYeMnWnyYx4h-HDdO0yFORIl6fpdxoBRxiJ5jAfia43TgeT34hH4iHvzB50x82Po0j1s-Z_vMl4E4-hRKfJ7XhPSWvdr7MdO7x3rNfn2--3n7tbr__uXb7af7Chtjl8p23oD3pumDaqXVIZBRxu516BX5tgFL2CgBCqhr0PYSbRcwdBq0Riv36pq9v-SWA3-vlBd3ihlpHMuy85qd0ADWCm3t86hSrerAGl1Qc0ExzTkn2ruHFE8-bU6AOytyR_ekyJ0VOWhdUVQabx5nrOFE_f-2JycF-HgBqDzlT6TkMkaakPqYCBfXz_G5Gf8ADqGn7Q</recordid><startdate>20130501</startdate><enddate>20130501</enddate><creator>Liu, Rongming</creator><creator>Liang, Liya</creator><creator>Cao, Weijia</creator><creator>Wu, Mingke</creator><creator>Chen, Kequan</creator><creator>Ma, Jiangfeng</creator><creator>Jiang, Min</creator><creator>Wei, Ping</creator><creator>Ouyang, Pingkai</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>7QO</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20130501</creationdate><title>Succinate production by metabolically engineered Escherichia coli using sugarcane bagasse hydrolysate as the carbon source</title><author>Liu, Rongming ; Liang, Liya ; Cao, Weijia ; Wu, Mingke ; Chen, Kequan ; Ma, Jiangfeng ; Jiang, Min ; Wei, Ping ; Ouyang, Pingkai</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c467t-79a60aa64db38275bbe6367f5bd3ea8407ec431030e94c7d2c79bcb95055c72f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Anaerobiosis - drug effects</topic><topic>ATP</topic><topic>Bacillus subtilis</topic><topic>Batch Cell Culture Techniques</topic><topic>Bioreactors - microbiology</topic><topic>Carbohydrates - pharmacology</topic><topic>Carbon - pharmacology</topic><topic>Cellulose - pharmacology</topic><topic>Escherichia coli</topic><topic>Escherichia coli - drug effects</topic><topic>Escherichia coli - metabolism</topic><topic>Escherichia coli BA204</topic><topic>Fermentation - drug effects</topic><topic>Hydrolysis - drug effects</topic><topic>Metabolic Engineering - methods</topic><topic>Saccharum - chemistry</topic><topic>Succinate</topic><topic>Succinic Acid - metabolism</topic><topic>Sugarcane bagasse hydrolysate</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Rongming</creatorcontrib><creatorcontrib>Liang, Liya</creatorcontrib><creatorcontrib>Cao, Weijia</creatorcontrib><creatorcontrib>Wu, Mingke</creatorcontrib><creatorcontrib>Chen, Kequan</creatorcontrib><creatorcontrib>Ma, Jiangfeng</creatorcontrib><creatorcontrib>Jiang, Min</creatorcontrib><creatorcontrib>Wei, Ping</creatorcontrib><creatorcontrib>Ouyang, Pingkai</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Bioresource technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Rongming</au><au>Liang, Liya</au><au>Cao, Weijia</au><au>Wu, Mingke</au><au>Chen, Kequan</au><au>Ma, Jiangfeng</au><au>Jiang, Min</au><au>Wei, Ping</au><au>Ouyang, Pingkai</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Succinate production by metabolically engineered Escherichia coli using sugarcane bagasse hydrolysate as the carbon source</atitle><jtitle>Bioresource technology</jtitle><addtitle>Bioresour Technol</addtitle><date>2013-05-01</date><risdate>2013</risdate><volume>135</volume><spage>574</spage><epage>577</epage><pages>574-577</pages><issn>0960-8524</issn><eissn>1873-2976</eissn><abstract>► Enhancement of ATP supply increased succinate production in Escherichia coli BA204. ► Engineered E. coli was constructed for sugarcane bagasse hydrolysate utilization. ► Fermentation of sugarcane bagasse hydrolysate to produce succinate was studied.
Efficient biosynthesis of succinate from a renewable biomass resource by engineered Escherichia coli is reported in this paper. Fermentation of sugarcane bagasse hydrolysate by engineered E. coli BA204, a pflB, ldhA, and ppc deletion strain overexpressing the ATP-forming phosphoenolpyruvate carboxykinase from Bacillus subtilis 168, produced a final succinate concentration of 15.85gL−1 with a high yield of 0.89gL−1 total sugar under anaerobic conditions. During dual-phase fermentations, initial aerobic growth facilitated subsequent anaerobic succinate production, with a final succinate concentration of 18.88gL−1 and a yield of 0.96gg−1 total sugar after 24h of anaerobic fermentation. The high succinate yield from sugarcane bagasse hydrolysate demonstrated a great potential application of renewable biomass as a feedstock for the economical production of succinate using metabolically engineered E. coli.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>23010211</pmid><doi>10.1016/j.biortech.2012.08.120</doi><tpages>4</tpages></addata></record> |
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| subjects | Anaerobiosis - drug effects ATP Bacillus subtilis Batch Cell Culture Techniques Bioreactors - microbiology Carbohydrates - pharmacology Carbon - pharmacology Cellulose - pharmacology Escherichia coli Escherichia coli - drug effects Escherichia coli - metabolism Escherichia coli BA204 Fermentation - drug effects Hydrolysis - drug effects Metabolic Engineering - methods Saccharum - chemistry Succinate Succinic Acid - metabolism Sugarcane bagasse hydrolysate |
| title | Succinate production by metabolically engineered Escherichia coli using sugarcane bagasse hydrolysate as the carbon source |
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