Repetitive domestication to enhance butanol tolerance and production in Clostridium acetobutylicum through artificial simulation of bio-evolution
► A novel approach was developed on the basis of dynamics of evolution and selection. ► A butanol-tolerant strain with increased butanol tolerance was obtained. ► Artificial simulation of bio-evolution (ASBE) was employed in biotechnology. ► The efficiency of ASBE was evaluated. ► The relationship b...
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| Veröffentlicht in: | Bioresource technology 2013-02, Vol.130, p.638-643 |
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| creator | Liu, Xiao-Bo Gu, Qiu-Ya Yu, Xiao-Bin |
| description | ► A novel approach was developed on the basis of dynamics of evolution and selection. ► A butanol-tolerant strain with increased butanol tolerance was obtained. ► Artificial simulation of bio-evolution (ASBE) was employed in biotechnology. ► The efficiency of ASBE was evaluated. ► The relationship between butanol tolerance and ABE production was investigated.
To improve butanol tolerance and production in Clostridium acetobutylicum, a novel approach was developed in this study, which was called artificial simulation of bio-evolution (ASBE) based on the evolutionary dynamics and natural selection. Through repetitive evolutionary domestications, a butanol-tolerant strain C. acetobutylicum T64 was obtained, which could withstand 4% (v/v) (compared to 2% of the wild-type) butanol and was accompanied by the increase of butanol production from 12.2g/L to 15.3g/L using corn meal as substrate. Fermentation was also carried out to investigate the relationship between butanol tolerance and ABE production, suggesting that enhancing butanol tolerance could increase butanol production but unlikely improve total ABE production. These results also indicated that the ASBE would be an available and feasible method used in biotechnology for enhancement of butanol tolerance and production. |
| doi_str_mv | 10.1016/j.biortech.2012.12.121 |
| format | Article |
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To improve butanol tolerance and production in Clostridium acetobutylicum, a novel approach was developed in this study, which was called artificial simulation of bio-evolution (ASBE) based on the evolutionary dynamics and natural selection. Through repetitive evolutionary domestications, a butanol-tolerant strain C. acetobutylicum T64 was obtained, which could withstand 4% (v/v) (compared to 2% of the wild-type) butanol and was accompanied by the increase of butanol production from 12.2g/L to 15.3g/L using corn meal as substrate. Fermentation was also carried out to investigate the relationship between butanol tolerance and ABE production, suggesting that enhancing butanol tolerance could increase butanol production but unlikely improve total ABE production. These results also indicated that the ASBE would be an available and feasible method used in biotechnology for enhancement of butanol tolerance and production.</description><identifier>ISSN: 0960-8524</identifier><identifier>EISSN: 1873-2976</identifier><identifier>DOI: 10.1016/j.biortech.2012.12.121</identifier><identifier>PMID: 23334021</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>ABE production ; Biofuel ; Biofuel production ; Biofuels ; Biological and medical sciences ; Biological Evolution ; Biotechnology ; Butanol ; Butanol tolerance ; Butanols - toxicity ; Cell Proliferation ; Clostridium acetobutylicum ; Clostridium acetobutylicum - cytology ; Clostridium acetobutylicum - drug effects ; Clostridium acetobutylicum - genetics ; Corn ; Domestication ; Dynamic tests ; Energy ; Evolutionary ; Fundamental and applied biological sciences. Psychology ; Industrial applications and implications. Economical aspects ; Kinetics ; Selection, Genetic ; Simulation ; Tolerances</subject><ispartof>Bioresource technology, 2013-02, Vol.130, p.638-643</ispartof><rights>2012 Elsevier Ltd</rights><rights>2014 INIST-CNRS</rights><rights>Copyright © 2012 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c497t-4fb829c6550b5070c6aec697f7a507de88df52f0e94bc4fa3311eacfca0e759f3</citedby><cites>FETCH-LOGICAL-c497t-4fb829c6550b5070c6aec697f7a507de88df52f0e94bc4fa3311eacfca0e759f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0960852412019797$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=27141411$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23334021$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Xiao-Bo</creatorcontrib><creatorcontrib>Gu, Qiu-Ya</creatorcontrib><creatorcontrib>Yu, Xiao-Bin</creatorcontrib><title>Repetitive domestication to enhance butanol tolerance and production in Clostridium acetobutylicum through artificial simulation of bio-evolution</title><title>Bioresource technology</title><addtitle>Bioresour Technol</addtitle><description>► A novel approach was developed on the basis of dynamics of evolution and selection. ► A butanol-tolerant strain with increased butanol tolerance was obtained. ► Artificial simulation of bio-evolution (ASBE) was employed in biotechnology. ► The efficiency of ASBE was evaluated. ► The relationship between butanol tolerance and ABE production was investigated.
To improve butanol tolerance and production in Clostridium acetobutylicum, a novel approach was developed in this study, which was called artificial simulation of bio-evolution (ASBE) based on the evolutionary dynamics and natural selection. Through repetitive evolutionary domestications, a butanol-tolerant strain C. acetobutylicum T64 was obtained, which could withstand 4% (v/v) (compared to 2% of the wild-type) butanol and was accompanied by the increase of butanol production from 12.2g/L to 15.3g/L using corn meal as substrate. Fermentation was also carried out to investigate the relationship between butanol tolerance and ABE production, suggesting that enhancing butanol tolerance could increase butanol production but unlikely improve total ABE production. These results also indicated that the ASBE would be an available and feasible method used in biotechnology for enhancement of butanol tolerance and production.</description><subject>ABE production</subject><subject>Biofuel</subject><subject>Biofuel production</subject><subject>Biofuels</subject><subject>Biological and medical sciences</subject><subject>Biological Evolution</subject><subject>Biotechnology</subject><subject>Butanol</subject><subject>Butanol tolerance</subject><subject>Butanols - toxicity</subject><subject>Cell Proliferation</subject><subject>Clostridium acetobutylicum</subject><subject>Clostridium acetobutylicum - cytology</subject><subject>Clostridium acetobutylicum - drug effects</subject><subject>Clostridium acetobutylicum - genetics</subject><subject>Corn</subject><subject>Domestication</subject><subject>Dynamic tests</subject><subject>Energy</subject><subject>Evolutionary</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Industrial applications and implications. Economical aspects</subject><subject>Kinetics</subject><subject>Selection, Genetic</subject><subject>Simulation</subject><subject>Tolerances</subject><issn>0960-8524</issn><issn>1873-2976</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkduKFDEQhoMo7rj6CktuBG96TNKHdO6UZT3AgiB6HdLpilNDujMm6YF9DN_Y9MysXi4pCFV8VZU_PyE3nG054937_XbAEDPY3VYwLran4M_IhveyroSS3XOyYapjVd-K5oq8SmnPGKu5FC_JlajrumGCb8if73CAjBmPQMcwQcpoTcYw0xwozDszW6DDks0cfCl5iKeKmUd6iGFc7InFmd76kHLEEZeJGgs5lKYHj7akeRfD8mtHTczo0KLxNOG0-POe4GiRUsEx-GUtvCYvnPEJ3lzua_Lz092P2y_V_bfPX28_3le2UTJXjRt6oWzXtmxomWS2M2A7JZ00JR2h70fXCsdANYNtnKlrzsFYZw0D2SpXX5N357lFx--lCNcTJgvemxnCkjRvGZOdUkw8jYpeNVJIuaLdGbUxpBTB6UPEycQHzZlendN7_eicXp3Tp-Cl8eayYxkmGP-1PVpVgLcXwCRrvFuNwPSfk7wpZ-U-nDkon3dEiDpZhGLaiBFs1mPAp97yFx-ivyU</recordid><startdate>20130201</startdate><enddate>20130201</enddate><creator>Liu, Xiao-Bo</creator><creator>Gu, Qiu-Ya</creator><creator>Yu, Xiao-Bin</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7SU</scope><scope>7TB</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>KR7</scope></search><sort><creationdate>20130201</creationdate><title>Repetitive domestication to enhance butanol tolerance and production in Clostridium acetobutylicum through artificial simulation of bio-evolution</title><author>Liu, Xiao-Bo ; Gu, Qiu-Ya ; Yu, Xiao-Bin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c497t-4fb829c6550b5070c6aec697f7a507de88df52f0e94bc4fa3311eacfca0e759f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>ABE production</topic><topic>Biofuel</topic><topic>Biofuel production</topic><topic>Biofuels</topic><topic>Biological and medical sciences</topic><topic>Biological Evolution</topic><topic>Biotechnology</topic><topic>Butanol</topic><topic>Butanol tolerance</topic><topic>Butanols - toxicity</topic><topic>Cell Proliferation</topic><topic>Clostridium acetobutylicum</topic><topic>Clostridium acetobutylicum - cytology</topic><topic>Clostridium acetobutylicum - drug effects</topic><topic>Clostridium acetobutylicum - genetics</topic><topic>Corn</topic><topic>Domestication</topic><topic>Dynamic tests</topic><topic>Energy</topic><topic>Evolutionary</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Industrial applications and implications. Economical aspects</topic><topic>Kinetics</topic><topic>Selection, Genetic</topic><topic>Simulation</topic><topic>Tolerances</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Xiao-Bo</creatorcontrib><creatorcontrib>Gu, Qiu-Ya</creatorcontrib><creatorcontrib>Yu, Xiao-Bin</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Environmental Engineering Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Civil Engineering Abstracts</collection><jtitle>Bioresource technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Xiao-Bo</au><au>Gu, Qiu-Ya</au><au>Yu, Xiao-Bin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Repetitive domestication to enhance butanol tolerance and production in Clostridium acetobutylicum through artificial simulation of bio-evolution</atitle><jtitle>Bioresource technology</jtitle><addtitle>Bioresour Technol</addtitle><date>2013-02-01</date><risdate>2013</risdate><volume>130</volume><spage>638</spage><epage>643</epage><pages>638-643</pages><issn>0960-8524</issn><eissn>1873-2976</eissn><abstract>► A novel approach was developed on the basis of dynamics of evolution and selection. ► A butanol-tolerant strain with increased butanol tolerance was obtained. ► Artificial simulation of bio-evolution (ASBE) was employed in biotechnology. ► The efficiency of ASBE was evaluated. ► The relationship between butanol tolerance and ABE production was investigated.
To improve butanol tolerance and production in Clostridium acetobutylicum, a novel approach was developed in this study, which was called artificial simulation of bio-evolution (ASBE) based on the evolutionary dynamics and natural selection. Through repetitive evolutionary domestications, a butanol-tolerant strain C. acetobutylicum T64 was obtained, which could withstand 4% (v/v) (compared to 2% of the wild-type) butanol and was accompanied by the increase of butanol production from 12.2g/L to 15.3g/L using corn meal as substrate. Fermentation was also carried out to investigate the relationship between butanol tolerance and ABE production, suggesting that enhancing butanol tolerance could increase butanol production but unlikely improve total ABE production. These results also indicated that the ASBE would be an available and feasible method used in biotechnology for enhancement of butanol tolerance and production.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>23334021</pmid><doi>10.1016/j.biortech.2012.12.121</doi><tpages>6</tpages></addata></record> |
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| subjects | ABE production Biofuel Biofuel production Biofuels Biological and medical sciences Biological Evolution Biotechnology Butanol Butanol tolerance Butanols - toxicity Cell Proliferation Clostridium acetobutylicum Clostridium acetobutylicum - cytology Clostridium acetobutylicum - drug effects Clostridium acetobutylicum - genetics Corn Domestication Dynamic tests Energy Evolutionary Fundamental and applied biological sciences. Psychology Industrial applications and implications. Economical aspects Kinetics Selection, Genetic Simulation Tolerances |
| title | Repetitive domestication to enhance butanol tolerance and production in Clostridium acetobutylicum through artificial simulation of bio-evolution |
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