Comparative study on ethanol production from pretreated sugarcane bagasse using immobilized Saccharomyces cerevisiae on various matrices
Microwave alkali pretreated sugarcane bagasse was used as a substrate for production of cellulolytic enzymes, needed for biomass hydrolysis. The pretreated sugarcane bagasse was enzymatic hydrolyzed by crude unprocessed enzymes cellulase (Filter paper activity 9.4 FPU/g), endoglucanase (carboxymethy...
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| Veröffentlicht in: | Renewable energy 2013-02, Vol.50, p.488-493 |
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| creator | Singh, Anita Sharma, Punita Saran, Alok Kumar Singh, Namita Bishnoi, Narsi R. |
| description | Microwave alkali pretreated sugarcane bagasse was used as a substrate for production of cellulolytic enzymes, needed for biomass hydrolysis. The pretreated sugarcane bagasse was enzymatic hydrolyzed by crude unprocessed enzymes cellulase (Filter paper activity 9.4 FPU/g), endoglucanase (carboxymethylcellulase, 148 IU/g), β-glucosidase (116 IU/g) and xylanase (201 IU/g) produced by Aspergillus flavus using pretreated sugarcane bagasse as substrate under solid state fermentation. Concentrated enzymatic hydrolyzate was used for ethanol production using Saccharomyces cerevisiae immobilized on various matrices. The yield of ethanol was 0.44 gp/gs in case of yeast immobilized sugarcane bagasse, 0.38 gp/gs using Ca-alginate and 0.33 gp/gs using agar-agar as immobilization matrices. The immobilized yeast studied up to 10 cycles in case of immobilized sugarcane bagasse and up to 4 cycles in case of agar-agar and calcium alginate for ethanol production under repeated batch fermentation study.
► Ethanol production from microwave alkali pretreated sugarcane bagasse. ► On- site hydrolytic enzymes produced using pretreated sugarcane bagasse by Aspergillus flavus. ► Enzymatic hydrolysis by crude unprocessed hydrolytic enzymes. ► Comparison of three immobilization matrices for yeast for ethanol production. |
| doi_str_mv | 10.1016/j.renene.2012.07.003 |
| format | Article |
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► Ethanol production from microwave alkali pretreated sugarcane bagasse. ► On- site hydrolytic enzymes produced using pretreated sugarcane bagasse by Aspergillus flavus. ► Enzymatic hydrolysis by crude unprocessed hydrolytic enzymes. ► Comparison of three immobilization matrices for yeast for ethanol production.</description><identifier>ISSN: 0960-1481</identifier><identifier>EISSN: 1879-0682</identifier><identifier>DOI: 10.1016/j.renene.2012.07.003</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Applied sciences ; Aspergillus flavus ; Bagasse ; batch fermentation ; beta-glucosidase ; Biomass ; calcium alginate ; endo-1,4-beta-glucanase ; Energy ; Enzymes ; Ethanol ; ethanol production ; Ethyl alcohol ; Exact sciences and technology ; Fermentation ; hydrolysates ; hydrolysis ; Immobilization ; Natural energy ; renewable energy sources ; Saccharomyces cerevisiae ; solid state fermentation ; Sugarcane bagasse ; xylanases ; Yeast ; yeasts</subject><ispartof>Renewable energy, 2013-02, Vol.50, p.488-493</ispartof><rights>2012 Elsevier Ltd</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c459t-f10aa4f6e01ac75704eda4f87d06d462710e017e016eb1b1f855b560dedde13a3</citedby><cites>FETCH-LOGICAL-c459t-f10aa4f6e01ac75704eda4f87d06d462710e017e016eb1b1f855b560dedde13a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0960148112004296$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26585562$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Singh, Anita</creatorcontrib><creatorcontrib>Sharma, Punita</creatorcontrib><creatorcontrib>Saran, Alok Kumar</creatorcontrib><creatorcontrib>Singh, Namita</creatorcontrib><creatorcontrib>Bishnoi, Narsi R.</creatorcontrib><title>Comparative study on ethanol production from pretreated sugarcane bagasse using immobilized Saccharomyces cerevisiae on various matrices</title><title>Renewable energy</title><description>Microwave alkali pretreated sugarcane bagasse was used as a substrate for production of cellulolytic enzymes, needed for biomass hydrolysis. The pretreated sugarcane bagasse was enzymatic hydrolyzed by crude unprocessed enzymes cellulase (Filter paper activity 9.4 FPU/g), endoglucanase (carboxymethylcellulase, 148 IU/g), β-glucosidase (116 IU/g) and xylanase (201 IU/g) produced by Aspergillus flavus using pretreated sugarcane bagasse as substrate under solid state fermentation. Concentrated enzymatic hydrolyzate was used for ethanol production using Saccharomyces cerevisiae immobilized on various matrices. The yield of ethanol was 0.44 gp/gs in case of yeast immobilized sugarcane bagasse, 0.38 gp/gs using Ca-alginate and 0.33 gp/gs using agar-agar as immobilization matrices. The immobilized yeast studied up to 10 cycles in case of immobilized sugarcane bagasse and up to 4 cycles in case of agar-agar and calcium alginate for ethanol production under repeated batch fermentation study.
► Ethanol production from microwave alkali pretreated sugarcane bagasse. ► On- site hydrolytic enzymes produced using pretreated sugarcane bagasse by Aspergillus flavus. ► Enzymatic hydrolysis by crude unprocessed hydrolytic enzymes. ► Comparison of three immobilization matrices for yeast for ethanol production.</description><subject>Applied sciences</subject><subject>Aspergillus flavus</subject><subject>Bagasse</subject><subject>batch fermentation</subject><subject>beta-glucosidase</subject><subject>Biomass</subject><subject>calcium alginate</subject><subject>endo-1,4-beta-glucanase</subject><subject>Energy</subject><subject>Enzymes</subject><subject>Ethanol</subject><subject>ethanol production</subject><subject>Ethyl alcohol</subject><subject>Exact sciences and technology</subject><subject>Fermentation</subject><subject>hydrolysates</subject><subject>hydrolysis</subject><subject>Immobilization</subject><subject>Natural energy</subject><subject>renewable energy sources</subject><subject>Saccharomyces cerevisiae</subject><subject>solid state fermentation</subject><subject>Sugarcane bagasse</subject><subject>xylanases</subject><subject>Yeast</subject><subject>yeasts</subject><issn>0960-1481</issn><issn>1879-0682</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><recordid>eNp9kE2LFDEQhhtRcFz9B4K5CF66rfR0kp6LIINfsOBh3XOoTqpnM_THmKQHxl_gz7bGXjxKCKFST1W99RbFawmVBKnfH6tIE5-qBllXYCqA7ZNiI1uzK0G39dNiAzsNpWxa-bx4kdIRQKrWNJvi934eTxgxhzOJlBd_EfMkKD_gNA_iFGe_uBz4q4_zyDHlSJjJi7QcMDqcSHR4wJRILClMBxHGce7CEH4xc4fOPSAXXhwl4SjSOaSAdB1xxhjmJYkRcwycflk863FI9OrxvSnuP3_6sf9a3n7_8m3_8bZ0jdrlspeA2PSaQKIzykBDnuPWeNC-0bWRwCnDV1MnO9m3SnVKgyfvSW5xe1O8W_vybj8XStmOITkaBl6F9VipAAx7pjSjzYq6OKcUqbenGEaMFyvBXo23R7sab6_GWzCWjeeyt48TMDkc-oiTC-lfba0Va9I1c29WrsfZ4iEyc3_HjViANKD-CviwEsSGnANFm1ygyZEPkVy2fg7_l_IHL1WoXg</recordid><startdate>20130201</startdate><enddate>20130201</enddate><creator>Singh, Anita</creator><creator>Sharma, Punita</creator><creator>Saran, Alok Kumar</creator><creator>Singh, Namita</creator><creator>Bishnoi, Narsi R.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SU</scope><scope>7TB</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H8D</scope><scope>KR7</scope><scope>L7M</scope></search><sort><creationdate>20130201</creationdate><title>Comparative study on ethanol production from pretreated sugarcane bagasse using immobilized Saccharomyces cerevisiae on various matrices</title><author>Singh, Anita ; Sharma, Punita ; Saran, Alok Kumar ; Singh, Namita ; Bishnoi, Narsi R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c459t-f10aa4f6e01ac75704eda4f87d06d462710e017e016eb1b1f855b560dedde13a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Applied sciences</topic><topic>Aspergillus flavus</topic><topic>Bagasse</topic><topic>batch fermentation</topic><topic>beta-glucosidase</topic><topic>Biomass</topic><topic>calcium alginate</topic><topic>endo-1,4-beta-glucanase</topic><topic>Energy</topic><topic>Enzymes</topic><topic>Ethanol</topic><topic>ethanol production</topic><topic>Ethyl alcohol</topic><topic>Exact sciences and technology</topic><topic>Fermentation</topic><topic>hydrolysates</topic><topic>hydrolysis</topic><topic>Immobilization</topic><topic>Natural energy</topic><topic>renewable energy sources</topic><topic>Saccharomyces cerevisiae</topic><topic>solid state fermentation</topic><topic>Sugarcane bagasse</topic><topic>xylanases</topic><topic>Yeast</topic><topic>yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Singh, Anita</creatorcontrib><creatorcontrib>Sharma, Punita</creatorcontrib><creatorcontrib>Saran, Alok Kumar</creatorcontrib><creatorcontrib>Singh, Namita</creatorcontrib><creatorcontrib>Bishnoi, Narsi R.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Environmental Engineering Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Renewable energy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Singh, Anita</au><au>Sharma, Punita</au><au>Saran, Alok Kumar</au><au>Singh, Namita</au><au>Bishnoi, Narsi R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparative study on ethanol production from pretreated sugarcane bagasse using immobilized Saccharomyces cerevisiae on various matrices</atitle><jtitle>Renewable energy</jtitle><date>2013-02-01</date><risdate>2013</risdate><volume>50</volume><spage>488</spage><epage>493</epage><pages>488-493</pages><issn>0960-1481</issn><eissn>1879-0682</eissn><abstract>Microwave alkali pretreated sugarcane bagasse was used as a substrate for production of cellulolytic enzymes, needed for biomass hydrolysis. The pretreated sugarcane bagasse was enzymatic hydrolyzed by crude unprocessed enzymes cellulase (Filter paper activity 9.4 FPU/g), endoglucanase (carboxymethylcellulase, 148 IU/g), β-glucosidase (116 IU/g) and xylanase (201 IU/g) produced by Aspergillus flavus using pretreated sugarcane bagasse as substrate under solid state fermentation. Concentrated enzymatic hydrolyzate was used for ethanol production using Saccharomyces cerevisiae immobilized on various matrices. The yield of ethanol was 0.44 gp/gs in case of yeast immobilized sugarcane bagasse, 0.38 gp/gs using Ca-alginate and 0.33 gp/gs using agar-agar as immobilization matrices. The immobilized yeast studied up to 10 cycles in case of immobilized sugarcane bagasse and up to 4 cycles in case of agar-agar and calcium alginate for ethanol production under repeated batch fermentation study.
► Ethanol production from microwave alkali pretreated sugarcane bagasse. ► On- site hydrolytic enzymes produced using pretreated sugarcane bagasse by Aspergillus flavus. ► Enzymatic hydrolysis by crude unprocessed hydrolytic enzymes. ► Comparison of three immobilization matrices for yeast for ethanol production.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><doi>10.1016/j.renene.2012.07.003</doi><tpages>6</tpages></addata></record> |
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| subjects | Applied sciences Aspergillus flavus Bagasse batch fermentation beta-glucosidase Biomass calcium alginate endo-1,4-beta-glucanase Energy Enzymes Ethanol ethanol production Ethyl alcohol Exact sciences and technology Fermentation hydrolysates hydrolysis Immobilization Natural energy renewable energy sources Saccharomyces cerevisiae solid state fermentation Sugarcane bagasse xylanases Yeast yeasts |
| title | Comparative study on ethanol production from pretreated sugarcane bagasse using immobilized Saccharomyces cerevisiae on various matrices |
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