Enhanced butanol production by coculture of Clostridium beijerinckii and Clostridium tyrobutyricum
•Free and immobilized-cell coculture greatly improved butanol productivity.•The highest ABE productivity was obtained in continuous immobilized-cell coculture.•This continuous coculture may be suitable for industrial ABE production. Cocultures of Clostridium beijerinckii and Clostridium tyrobutyricu...
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| Veröffentlicht in: | Bioresource technology 2013-09, Vol.143, p.397-404 |
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| creator | Li, Lin Ai, Hongxia Zhang, Shexi Li, Shuang Liang, Zexin Wu, Zhen-Qiang Yang, Shang-Tian Wang, Ju-Fang |
| description | •Free and immobilized-cell coculture greatly improved butanol productivity.•The highest ABE productivity was obtained in continuous immobilized-cell coculture.•This continuous coculture may be suitable for industrial ABE production.
Cocultures of Clostridium beijerinckii and Clostridium tyrobutyricum in free-cell and immobilized-cell fermentation modes were investigated as a means of enhancing butanol production. The immobilized fermentation was performed in a fibrous-bed bioreactor (FBB). The results demonstrated that two-strain coculture significantly enhanced butanol production, yield and volumetric productivity compared with those in pure culture with or without butyric acid. Further, continuous immobilized-cell cocultures in two FBBs using glucose, cassava starch, or cane molasses were conducted at a dilution rate of 0.144h−1. The butanol production (6.66g/L), yield (0.18g/g), and productivity (0.96g/L/h) were obtained with cassava starch as the substrate. Meanwhile, the acetone–butanol–ethanol (ABE) yield (0.36g/g) was the highest among all processes investigated, suggesting that this continuous coculture mode may be suitable for industrial ABE production with no need for repeated sterilization and inoculation. |
| doi_str_mv | 10.1016/j.biortech.2013.06.023 |
| format | Article |
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Cocultures of Clostridium beijerinckii and Clostridium tyrobutyricum in free-cell and immobilized-cell fermentation modes were investigated as a means of enhancing butanol production. The immobilized fermentation was performed in a fibrous-bed bioreactor (FBB). The results demonstrated that two-strain coculture significantly enhanced butanol production, yield and volumetric productivity compared with those in pure culture with or without butyric acid. Further, continuous immobilized-cell cocultures in two FBBs using glucose, cassava starch, or cane molasses were conducted at a dilution rate of 0.144h−1. The butanol production (6.66g/L), yield (0.18g/g), and productivity (0.96g/L/h) were obtained with cassava starch as the substrate. Meanwhile, the acetone–butanol–ethanol (ABE) yield (0.36g/g) was the highest among all processes investigated, suggesting that this continuous coculture mode may be suitable for industrial ABE production with no need for repeated sterilization and inoculation.</description><identifier>ISSN: 0960-8524</identifier><identifier>EISSN: 1873-2976</identifier><identifier>DOI: 10.1016/j.biortech.2013.06.023</identifier><identifier>PMID: 23819976</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>Acetone–butanol–ethanol fermentation ; Biological and medical sciences ; Bioreactors ; Biotechnology ; Butanol ; Butanols - metabolism ; Cassava ; Clostridium ; Clostridium - classification ; Clostridium - metabolism ; Clostridium tyrobutyricum ; Coculture ; Coculture Techniques ; Culture ; Fermentation ; Fibrous-bed bioreactor ; Free-cell fermentation ; Fundamental and applied biological sciences. Psychology ; Immobilized-cell fermentation ; Methods. Procedures. Technologies ; Microbial engineering. Fermentation and microbial culture technology ; Productivity ; Species Specificity ; Starches ; Various methods and equipments</subject><ispartof>Bioresource technology, 2013-09, Vol.143, p.397-404</ispartof><rights>2013 Elsevier Ltd</rights><rights>2014 INIST-CNRS</rights><rights>Copyright © 2013 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c497t-a49e2e1e504fa91851204390dc8c2b23451e9669f1d127f8e89866fbfad35a583</citedby><cites>FETCH-LOGICAL-c497t-a49e2e1e504fa91851204390dc8c2b23451e9669f1d127f8e89866fbfad35a583</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0960852413009425$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=27614654$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23819976$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Lin</creatorcontrib><creatorcontrib>Ai, Hongxia</creatorcontrib><creatorcontrib>Zhang, Shexi</creatorcontrib><creatorcontrib>Li, Shuang</creatorcontrib><creatorcontrib>Liang, Zexin</creatorcontrib><creatorcontrib>Wu, Zhen-Qiang</creatorcontrib><creatorcontrib>Yang, Shang-Tian</creatorcontrib><creatorcontrib>Wang, Ju-Fang</creatorcontrib><title>Enhanced butanol production by coculture of Clostridium beijerinckii and Clostridium tyrobutyricum</title><title>Bioresource technology</title><addtitle>Bioresour Technol</addtitle><description>•Free and immobilized-cell coculture greatly improved butanol productivity.•The highest ABE productivity was obtained in continuous immobilized-cell coculture.•This continuous coculture may be suitable for industrial ABE production.
Cocultures of Clostridium beijerinckii and Clostridium tyrobutyricum in free-cell and immobilized-cell fermentation modes were investigated as a means of enhancing butanol production. The immobilized fermentation was performed in a fibrous-bed bioreactor (FBB). The results demonstrated that two-strain coculture significantly enhanced butanol production, yield and volumetric productivity compared with those in pure culture with or without butyric acid. Further, continuous immobilized-cell cocultures in two FBBs using glucose, cassava starch, or cane molasses were conducted at a dilution rate of 0.144h−1. The butanol production (6.66g/L), yield (0.18g/g), and productivity (0.96g/L/h) were obtained with cassava starch as the substrate. Meanwhile, the acetone–butanol–ethanol (ABE) yield (0.36g/g) was the highest among all processes investigated, suggesting that this continuous coculture mode may be suitable for industrial ABE production with no need for repeated sterilization and inoculation.</description><subject>Acetone–butanol–ethanol fermentation</subject><subject>Biological and medical sciences</subject><subject>Bioreactors</subject><subject>Biotechnology</subject><subject>Butanol</subject><subject>Butanols - metabolism</subject><subject>Cassava</subject><subject>Clostridium</subject><subject>Clostridium - classification</subject><subject>Clostridium - metabolism</subject><subject>Clostridium tyrobutyricum</subject><subject>Coculture</subject><subject>Coculture Techniques</subject><subject>Culture</subject><subject>Fermentation</subject><subject>Fibrous-bed bioreactor</subject><subject>Free-cell fermentation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Immobilized-cell fermentation</subject><subject>Methods. Procedures. Technologies</subject><subject>Microbial engineering. Fermentation and microbial culture technology</subject><subject>Productivity</subject><subject>Species Specificity</subject><subject>Starches</subject><subject>Various methods and equipments</subject><issn>0960-8524</issn><issn>1873-2976</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtv1DAQgC0EokvhL1S5IHFJ8NiOY99Aq_KQKnGBs-XYY9VLEhc7Rtp_T1a7BXHqaQ7zzfMj5AZoBxTk-0M3xpRXdPcdo8A7KjvK-DOyAzXwlulBPic7qiVtVc_EFXlVyoFSymFgL8kV4wr0xuzIeLvc28Whb8a62iVNzUNOvro1pqUZj41Lrk5rzdik0OynVNYcfaxzM2I8YI6L-xljYxf_X3I95rT1O-bo6vyavAh2KvjmEq_Jj0-33_df2rtvn7_uP961Tuhhba3QyBCwpyJYDaoHRgXX1Dvl2Mi46AG1lDqABzYEhUorKcMYrOe97RW_Ju_OfbcLflUsq5ljcThNdsFUi4Ge0kEOoOFpVAAH2XMtNlSeUZdTKRmDechxtvlogJqTCnMwjyrMSYWh0mwqtsKby4w6zuj_lj3-fgPeXgBbnJ1C3jzE8o8bJAjZnzb4cOZwe97viNkUF_HkLGZ0q_EpPrXLH1IAq9M</recordid><startdate>20130901</startdate><enddate>20130901</enddate><creator>Li, Lin</creator><creator>Ai, Hongxia</creator><creator>Zhang, Shexi</creator><creator>Li, Shuang</creator><creator>Liang, Zexin</creator><creator>Wu, Zhen-Qiang</creator><creator>Yang, Shang-Tian</creator><creator>Wang, Ju-Fang</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7SU</scope><scope>7TB</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>KR7</scope></search><sort><creationdate>20130901</creationdate><title>Enhanced butanol production by coculture of Clostridium beijerinckii and Clostridium tyrobutyricum</title><author>Li, Lin ; Ai, Hongxia ; Zhang, Shexi ; Li, Shuang ; Liang, Zexin ; Wu, Zhen-Qiang ; Yang, Shang-Tian ; Wang, Ju-Fang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c497t-a49e2e1e504fa91851204390dc8c2b23451e9669f1d127f8e89866fbfad35a583</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Acetone–butanol–ethanol fermentation</topic><topic>Biological and medical sciences</topic><topic>Bioreactors</topic><topic>Biotechnology</topic><topic>Butanol</topic><topic>Butanols - metabolism</topic><topic>Cassava</topic><topic>Clostridium</topic><topic>Clostridium - classification</topic><topic>Clostridium - metabolism</topic><topic>Clostridium tyrobutyricum</topic><topic>Coculture</topic><topic>Coculture Techniques</topic><topic>Culture</topic><topic>Fermentation</topic><topic>Fibrous-bed bioreactor</topic><topic>Free-cell fermentation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Immobilized-cell fermentation</topic><topic>Methods. Procedures. Technologies</topic><topic>Microbial engineering. Fermentation and microbial culture technology</topic><topic>Productivity</topic><topic>Species Specificity</topic><topic>Starches</topic><topic>Various methods and equipments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Lin</creatorcontrib><creatorcontrib>Ai, Hongxia</creatorcontrib><creatorcontrib>Zhang, Shexi</creatorcontrib><creatorcontrib>Li, Shuang</creatorcontrib><creatorcontrib>Liang, Zexin</creatorcontrib><creatorcontrib>Wu, Zhen-Qiang</creatorcontrib><creatorcontrib>Yang, Shang-Tian</creatorcontrib><creatorcontrib>Wang, Ju-Fang</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Environmental Engineering Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Civil Engineering Abstracts</collection><jtitle>Bioresource technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Lin</au><au>Ai, Hongxia</au><au>Zhang, Shexi</au><au>Li, Shuang</au><au>Liang, Zexin</au><au>Wu, Zhen-Qiang</au><au>Yang, Shang-Tian</au><au>Wang, Ju-Fang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enhanced butanol production by coculture of Clostridium beijerinckii and Clostridium tyrobutyricum</atitle><jtitle>Bioresource technology</jtitle><addtitle>Bioresour Technol</addtitle><date>2013-09-01</date><risdate>2013</risdate><volume>143</volume><spage>397</spage><epage>404</epage><pages>397-404</pages><issn>0960-8524</issn><eissn>1873-2976</eissn><abstract>•Free and immobilized-cell coculture greatly improved butanol productivity.•The highest ABE productivity was obtained in continuous immobilized-cell coculture.•This continuous coculture may be suitable for industrial ABE production.
Cocultures of Clostridium beijerinckii and Clostridium tyrobutyricum in free-cell and immobilized-cell fermentation modes were investigated as a means of enhancing butanol production. The immobilized fermentation was performed in a fibrous-bed bioreactor (FBB). The results demonstrated that two-strain coculture significantly enhanced butanol production, yield and volumetric productivity compared with those in pure culture with or without butyric acid. Further, continuous immobilized-cell cocultures in two FBBs using glucose, cassava starch, or cane molasses were conducted at a dilution rate of 0.144h−1. The butanol production (6.66g/L), yield (0.18g/g), and productivity (0.96g/L/h) were obtained with cassava starch as the substrate. Meanwhile, the acetone–butanol–ethanol (ABE) yield (0.36g/g) was the highest among all processes investigated, suggesting that this continuous coculture mode may be suitable for industrial ABE production with no need for repeated sterilization and inoculation.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>23819976</pmid><doi>10.1016/j.biortech.2013.06.023</doi><tpages>8</tpages></addata></record> |
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| ispartof | Bioresource technology, 2013-09, Vol.143, p.397-404 |
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| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Acetone–butanol–ethanol fermentation Biological and medical sciences Bioreactors Biotechnology Butanol Butanols - metabolism Cassava Clostridium Clostridium - classification Clostridium - metabolism Clostridium tyrobutyricum Coculture Coculture Techniques Culture Fermentation Fibrous-bed bioreactor Free-cell fermentation Fundamental and applied biological sciences. Psychology Immobilized-cell fermentation Methods. Procedures. Technologies Microbial engineering. Fermentation and microbial culture technology Productivity Species Specificity Starches Various methods and equipments |
| title | Enhanced butanol production by coculture of Clostridium beijerinckii and Clostridium tyrobutyricum |
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