Release of growth factors from a reinforced collagen GAG matrix supplemented with platelet rich plasma: Influence on cultured human meniscal cells
ABSTRACT Damage to meniscal cartilage has been strongly linked to accelerated articular wear and consequently to osteoarthritis. Damage might be ameliorated by delivery of growth factors from platelet rich plasma (PRP) via a fiber reinforced collagen matrix designed for meniscal repair. PRP composit...
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| Veröffentlicht in: | Journal of orthopaedic research 2014-02, Vol.32 (2), p.273-278 |
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| container_title | Journal of orthopaedic research |
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| creator | Howard, Daniel Shepherd, Jenny H. Kew, Simon J. Hernandez, Paula Ghose, Siddhartha Wardale, John A. Rushton, Neil |
| description | ABSTRACT
Damage to meniscal cartilage has been strongly linked to accelerated articular wear and consequently to osteoarthritis. Damage might be ameliorated by delivery of growth factors from platelet rich plasma (PRP) via a fiber reinforced collagen matrix designed for meniscal repair. PRP composition, release of growth factors, and influence on meniscal cell growth and gene expression were investigated. PRP was prepared using Harvest Smartprep (HS‐PRP), Cascade Fibrinet (CF‐PRP), and a simple centrifuge protocol (DC‐PRP) from four donors each. CF‐PRP had the highest ratio of platelets, with very few other blood cell types. HS‐PRP had the highest total number of platelets but also contained high levels of red and white blood cells. Absorbed to collagen matrices HS‐PRP released the highest levels of TGF‐β1 and PDGF‐AB with DC‐PRP the most IGF‐1. Cumulative release from collagen matrix was 48 ng/cm3 IGF‐1, 96 ng/cm3 TGF‐β1, and 9.6 ng/cm3 PDGF‐AB. Collagen matrix with PRP was able to increase meniscal cell number above peripheral whole blood and up‐regulated gene expression of Aggrecan, Collagen type I (α1), and Elastin (3.3 ± 0.8‐fold, 2.9 ± 0.6‐fold, 4.0 ± 1.4‐fold, respectively). Demonstrating that PRP combined with fiber reinforced collagen matrix could influence meniscal cells and might be of use for treating meniscal defects. © 2013 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 32:273–278, 2014. |
| doi_str_mv | 10.1002/jor.22495 |
| format | Article |
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Damage to meniscal cartilage has been strongly linked to accelerated articular wear and consequently to osteoarthritis. Damage might be ameliorated by delivery of growth factors from platelet rich plasma (PRP) via a fiber reinforced collagen matrix designed for meniscal repair. PRP composition, release of growth factors, and influence on meniscal cell growth and gene expression were investigated. PRP was prepared using Harvest Smartprep (HS‐PRP), Cascade Fibrinet (CF‐PRP), and a simple centrifuge protocol (DC‐PRP) from four donors each. CF‐PRP had the highest ratio of platelets, with very few other blood cell types. HS‐PRP had the highest total number of platelets but also contained high levels of red and white blood cells. Absorbed to collagen matrices HS‐PRP released the highest levels of TGF‐β1 and PDGF‐AB with DC‐PRP the most IGF‐1. Cumulative release from collagen matrix was 48 ng/cm3 IGF‐1, 96 ng/cm3 TGF‐β1, and 9.6 ng/cm3 PDGF‐AB. Collagen matrix with PRP was able to increase meniscal cell number above peripheral whole blood and up‐regulated gene expression of Aggrecan, Collagen type I (α1), and Elastin (3.3 ± 0.8‐fold, 2.9 ± 0.6‐fold, 4.0 ± 1.4‐fold, respectively). Demonstrating that PRP combined with fiber reinforced collagen matrix could influence meniscal cells and might be of use for treating meniscal defects. © 2013 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 32:273–278, 2014.</description><identifier>ISSN: 0736-0266</identifier><identifier>EISSN: 1554-527X</identifier><identifier>DOI: 10.1002/jor.22495</identifier><identifier>PMID: 24122924</identifier><language>eng</language><publisher>United States: Blackwell Publishing Ltd</publisher><subject>Cells, Cultured ; Collagen - metabolism ; Collagen Type I - biosynthesis ; Glycosaminoglycans ; Humans ; Insulin-Like Growth Factor I - biosynthesis ; meniscal cells ; meniscal repair ; Menisci, Tibial - cytology ; Menisci, Tibial - metabolism ; platelet rich plasma ; Platelet-Derived Growth Factor - biosynthesis ; Platelet-Rich Plasma - physiology ; PRP ; reinforced collagen matrix ; Tissue Scaffolds ; Transforming Growth Factor beta1 - biosynthesis</subject><ispartof>Journal of orthopaedic research, 2014-02, Vol.32 (2), p.273-278</ispartof><rights>2013 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3985-ab8696b3b59859020f00ec9e56243c5d8e639e91002eca7c6af109b528c787eb3</citedby><cites>FETCH-LOGICAL-c3985-ab8696b3b59859020f00ec9e56243c5d8e639e91002eca7c6af109b528c787eb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjor.22495$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjor.22495$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,1427,27901,27902,45550,45551,46384,46808</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24122924$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Howard, Daniel</creatorcontrib><creatorcontrib>Shepherd, Jenny H.</creatorcontrib><creatorcontrib>Kew, Simon J.</creatorcontrib><creatorcontrib>Hernandez, Paula</creatorcontrib><creatorcontrib>Ghose, Siddhartha</creatorcontrib><creatorcontrib>Wardale, John A.</creatorcontrib><creatorcontrib>Rushton, Neil</creatorcontrib><title>Release of growth factors from a reinforced collagen GAG matrix supplemented with platelet rich plasma: Influence on cultured human meniscal cells</title><title>Journal of orthopaedic research</title><addtitle>J. Orthop. Res</addtitle><description>ABSTRACT
Damage to meniscal cartilage has been strongly linked to accelerated articular wear and consequently to osteoarthritis. Damage might be ameliorated by delivery of growth factors from platelet rich plasma (PRP) via a fiber reinforced collagen matrix designed for meniscal repair. PRP composition, release of growth factors, and influence on meniscal cell growth and gene expression were investigated. PRP was prepared using Harvest Smartprep (HS‐PRP), Cascade Fibrinet (CF‐PRP), and a simple centrifuge protocol (DC‐PRP) from four donors each. CF‐PRP had the highest ratio of platelets, with very few other blood cell types. HS‐PRP had the highest total number of platelets but also contained high levels of red and white blood cells. Absorbed to collagen matrices HS‐PRP released the highest levels of TGF‐β1 and PDGF‐AB with DC‐PRP the most IGF‐1. Cumulative release from collagen matrix was 48 ng/cm3 IGF‐1, 96 ng/cm3 TGF‐β1, and 9.6 ng/cm3 PDGF‐AB. Collagen matrix with PRP was able to increase meniscal cell number above peripheral whole blood and up‐regulated gene expression of Aggrecan, Collagen type I (α1), and Elastin (3.3 ± 0.8‐fold, 2.9 ± 0.6‐fold, 4.0 ± 1.4‐fold, respectively). Demonstrating that PRP combined with fiber reinforced collagen matrix could influence meniscal cells and might be of use for treating meniscal defects. © 2013 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 32:273–278, 2014.</description><subject>Cells, Cultured</subject><subject>Collagen - metabolism</subject><subject>Collagen Type I - biosynthesis</subject><subject>Glycosaminoglycans</subject><subject>Humans</subject><subject>Insulin-Like Growth Factor I - biosynthesis</subject><subject>meniscal cells</subject><subject>meniscal repair</subject><subject>Menisci, Tibial - cytology</subject><subject>Menisci, Tibial - metabolism</subject><subject>platelet rich plasma</subject><subject>Platelet-Derived Growth Factor - biosynthesis</subject><subject>Platelet-Rich Plasma - physiology</subject><subject>PRP</subject><subject>reinforced collagen matrix</subject><subject>Tissue Scaffolds</subject><subject>Transforming Growth Factor beta1 - biosynthesis</subject><issn>0736-0266</issn><issn>1554-527X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kcFu1DAQhi0EokvhwAsgH-GQ1nZiO-ZWVTQUrSiqQCAuluMdtylOnNqOtn0Nnhi32_bGaTTS938zmkHoLSUHlBB2eBXiAWON4s_QinLeVJzJX8_RishaVIQJsYdepXRFCJGUtS_RHmsoY4o1K_T3HDyYBDg4fBHDNl9iZ2wOMWEXw4gNjjBMLkQLG2yD9-YCJtwddXg0OQ43OC3z7GGEKRdgO5T87E0u0ozjYO-7NJqP-HRyfoHJlkkTtovPSyyBy2U0Ey7pIVnjsQXv02v0whmf4M1D3Uc_Tj59P_5crc-60-OjdWVr1fLK9K1Qoq97XjpFGHGEgFXABWtqyzctiFqBujsQWCOtMI4S1XPWWtlK6Ot99H7nnWO4XiBlPZYtygZmgrAkTRulKKetaAv6YYfaGFKK4PQch9HEW02Jvpugywv0_QsK--5Bu_QjbJ7Ix5sX4HAHbAcPt_836S9n54_KapcYUoabp4SJf7SQteT659dO_ya0W39TJ1rW_wD486GO</recordid><startdate>201402</startdate><enddate>201402</enddate><creator>Howard, Daniel</creator><creator>Shepherd, Jenny H.</creator><creator>Kew, Simon J.</creator><creator>Hernandez, Paula</creator><creator>Ghose, Siddhartha</creator><creator>Wardale, John A.</creator><creator>Rushton, Neil</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201402</creationdate><title>Release of growth factors from a reinforced collagen GAG matrix supplemented with platelet rich plasma: Influence on cultured human meniscal cells</title><author>Howard, Daniel ; Shepherd, Jenny H. ; Kew, Simon J. ; Hernandez, Paula ; Ghose, Siddhartha ; Wardale, John A. ; Rushton, Neil</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3985-ab8696b3b59859020f00ec9e56243c5d8e639e91002eca7c6af109b528c787eb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Cells, Cultured</topic><topic>Collagen - metabolism</topic><topic>Collagen Type I - biosynthesis</topic><topic>Glycosaminoglycans</topic><topic>Humans</topic><topic>Insulin-Like Growth Factor I - biosynthesis</topic><topic>meniscal cells</topic><topic>meniscal repair</topic><topic>Menisci, Tibial - cytology</topic><topic>Menisci, Tibial - metabolism</topic><topic>platelet rich plasma</topic><topic>Platelet-Derived Growth Factor - biosynthesis</topic><topic>Platelet-Rich Plasma - physiology</topic><topic>PRP</topic><topic>reinforced collagen matrix</topic><topic>Tissue Scaffolds</topic><topic>Transforming Growth Factor beta1 - biosynthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Howard, Daniel</creatorcontrib><creatorcontrib>Shepherd, Jenny H.</creatorcontrib><creatorcontrib>Kew, Simon J.</creatorcontrib><creatorcontrib>Hernandez, Paula</creatorcontrib><creatorcontrib>Ghose, Siddhartha</creatorcontrib><creatorcontrib>Wardale, John A.</creatorcontrib><creatorcontrib>Rushton, Neil</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of orthopaedic research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Howard, Daniel</au><au>Shepherd, Jenny H.</au><au>Kew, Simon J.</au><au>Hernandez, Paula</au><au>Ghose, Siddhartha</au><au>Wardale, John A.</au><au>Rushton, Neil</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Release of growth factors from a reinforced collagen GAG matrix supplemented with platelet rich plasma: Influence on cultured human meniscal cells</atitle><jtitle>Journal of orthopaedic research</jtitle><addtitle>J. Orthop. Res</addtitle><date>2014-02</date><risdate>2014</risdate><volume>32</volume><issue>2</issue><spage>273</spage><epage>278</epage><pages>273-278</pages><issn>0736-0266</issn><eissn>1554-527X</eissn><abstract>ABSTRACT
Damage to meniscal cartilage has been strongly linked to accelerated articular wear and consequently to osteoarthritis. Damage might be ameliorated by delivery of growth factors from platelet rich plasma (PRP) via a fiber reinforced collagen matrix designed for meniscal repair. PRP composition, release of growth factors, and influence on meniscal cell growth and gene expression were investigated. PRP was prepared using Harvest Smartprep (HS‐PRP), Cascade Fibrinet (CF‐PRP), and a simple centrifuge protocol (DC‐PRP) from four donors each. CF‐PRP had the highest ratio of platelets, with very few other blood cell types. HS‐PRP had the highest total number of platelets but also contained high levels of red and white blood cells. Absorbed to collagen matrices HS‐PRP released the highest levels of TGF‐β1 and PDGF‐AB with DC‐PRP the most IGF‐1. Cumulative release from collagen matrix was 48 ng/cm3 IGF‐1, 96 ng/cm3 TGF‐β1, and 9.6 ng/cm3 PDGF‐AB. Collagen matrix with PRP was able to increase meniscal cell number above peripheral whole blood and up‐regulated gene expression of Aggrecan, Collagen type I (α1), and Elastin (3.3 ± 0.8‐fold, 2.9 ± 0.6‐fold, 4.0 ± 1.4‐fold, respectively). Demonstrating that PRP combined with fiber reinforced collagen matrix could influence meniscal cells and might be of use for treating meniscal defects. © 2013 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 32:273–278, 2014.</abstract><cop>United States</cop><pub>Blackwell Publishing Ltd</pub><pmid>24122924</pmid><doi>10.1002/jor.22495</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Cells, Cultured Collagen - metabolism Collagen Type I - biosynthesis Glycosaminoglycans Humans Insulin-Like Growth Factor I - biosynthesis meniscal cells meniscal repair Menisci, Tibial - cytology Menisci, Tibial - metabolism platelet rich plasma Platelet-Derived Growth Factor - biosynthesis Platelet-Rich Plasma - physiology PRP reinforced collagen matrix Tissue Scaffolds Transforming Growth Factor beta1 - biosynthesis |
| title | Release of growth factors from a reinforced collagen GAG matrix supplemented with platelet rich plasma: Influence on cultured human meniscal cells |
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