The frequency of BRO β-lactamase and its relationship to antimicrobial susceptibility and serum resistance in Moraxella catarrhalis
Abstract We investigated the frequency of BRO β-lactamase and its relationship to antibiotic susceptibility profiles and serum susceptibility. Moraxella catarrhalis clinical isolates ( n = 197) were collected from patients with respiratory tract infections in Tokyo between November 2004 and April 2...
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| Veröffentlicht in: | Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy 2014-01, Vol.20 (1), p.6-8 |
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| creator | Saito, Ryoichi Nonaka, Shotaro Fujinami, Yuji Matsuoka, Shiho Nakajima, Shinichi Nishiyama, Hiroyuki Okamura, Noboru |
| description | Abstract We investigated the frequency of BRO β-lactamase and its relationship to antibiotic susceptibility profiles and serum susceptibility. Moraxella catarrhalis clinical isolates ( n = 197) were collected from patients with respiratory tract infections in Tokyo between November 2004 and April 2005. Phenotypic and genotypic detection of β-lactamases was performed. The MICs of 6 antibiotics were determined by Etest, and the serum bactericidal assay was conducted by using the culture-and-spot test. Nearly all (192; 97%) of the clinical isolates were β-lactamase producers; of these, 182 (95%) were bro-1 and 10 (5%) were bro-2 positive. MIC50 , MIC90 , and geometric mean MICs of penicillin, amoxicillin, cefixime, and clarithromycin for BRO-1 isolates were significantly higher than for BRO-2 isolates. The frequency of intermediate and full serum resistance was significantly higher in BRO-1 isolates than in BRO-2 isolates ( P = 0.0056), but not BRO-negative isolates ( P = 0.1333). We provide the first evidence that the presence of BRO-1 in M. catarrhalis is associated with reduced susceptibility to clarithromycin and β-lactam antibiotics, as well as serum non-sensitive (intermediate and resistant). |
| doi_str_mv | 10.1016/j.jiac.2013.06.003 |
| format | Article |
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Moraxella catarrhalis clinical isolates ( n = 197) were collected from patients with respiratory tract infections in Tokyo between November 2004 and April 2005. Phenotypic and genotypic detection of β-lactamases was performed. The MICs of 6 antibiotics were determined by Etest, and the serum bactericidal assay was conducted by using the culture-and-spot test. Nearly all (192; 97%) of the clinical isolates were β-lactamase producers; of these, 182 (95%) were bro-1 and 10 (5%) were bro-2 positive. MIC50 , MIC90 , and geometric mean MICs of penicillin, amoxicillin, cefixime, and clarithromycin for BRO-1 isolates were significantly higher than for BRO-2 isolates. The frequency of intermediate and full serum resistance was significantly higher in BRO-1 isolates than in BRO-2 isolates ( P = 0.0056), but not BRO-negative isolates ( P = 0.1333). We provide the first evidence that the presence of BRO-1 in M. catarrhalis is associated with reduced susceptibility to clarithromycin and β-lactam antibiotics, as well as serum non-sensitive (intermediate and resistant).</description><identifier>ISSN: 1341-321X</identifier><identifier>EISSN: 1437-7780</identifier><identifier>DOI: 10.1016/j.jiac.2013.06.003</identifier><identifier>PMID: 24462416</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Anti-Infective Agents - pharmacology ; beta-Lactamases - analysis ; beta-Lactamases - biosynthesis ; Hematology, Oncology and Palliative Medicine ; Humans ; Macrolide ; Microbial Sensitivity Tests - methods ; Moraxella (Branhamella) catarrhalis - drug effects ; Moraxella (Branhamella) catarrhalis - enzymology ; Moraxella (Branhamella) catarrhalis - isolation & purification ; Moraxella catarrhalis ; Moraxellaceae Infections - drug therapy ; Moraxellaceae Infections - microbiology ; Respiratory Tract Infections - drug therapy ; Respiratory Tract Infections - microbiology ; Serum resistance ; β-Lactam ; β-Lactamase</subject><ispartof>Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy, 2014-01, Vol.20 (1), p.6-8</ispartof><rights>Japanese Society of Chemotherapy and the Japanese Association for Infectious Diseases</rights><rights>2013 Japanese Society of Chemotherapy and the Japanese Association for Infectious Diseases</rights><rights>Copyright © 2013 Japanese Society of Chemotherapy and the Japanese Association for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c505t-6d3027169180ccfa41aebdef3620378b657abc829aad2e0ad45f69222fec49923</citedby><cites>FETCH-LOGICAL-c505t-6d3027169180ccfa41aebdef3620378b657abc829aad2e0ad45f69222fec49923</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24462416$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Saito, Ryoichi</creatorcontrib><creatorcontrib>Nonaka, Shotaro</creatorcontrib><creatorcontrib>Fujinami, Yuji</creatorcontrib><creatorcontrib>Matsuoka, Shiho</creatorcontrib><creatorcontrib>Nakajima, Shinichi</creatorcontrib><creatorcontrib>Nishiyama, Hiroyuki</creatorcontrib><creatorcontrib>Okamura, Noboru</creatorcontrib><title>The frequency of BRO β-lactamase and its relationship to antimicrobial susceptibility and serum resistance in Moraxella catarrhalis</title><title>Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy</title><addtitle>J Infect Chemother</addtitle><description>Abstract We investigated the frequency of BRO β-lactamase and its relationship to antibiotic susceptibility profiles and serum susceptibility. Moraxella catarrhalis clinical isolates ( n = 197) were collected from patients with respiratory tract infections in Tokyo between November 2004 and April 2005. Phenotypic and genotypic detection of β-lactamases was performed. The MICs of 6 antibiotics were determined by Etest, and the serum bactericidal assay was conducted by using the culture-and-spot test. Nearly all (192; 97%) of the clinical isolates were β-lactamase producers; of these, 182 (95%) were bro-1 and 10 (5%) were bro-2 positive. MIC50 , MIC90 , and geometric mean MICs of penicillin, amoxicillin, cefixime, and clarithromycin for BRO-1 isolates were significantly higher than for BRO-2 isolates. The frequency of intermediate and full serum resistance was significantly higher in BRO-1 isolates than in BRO-2 isolates ( P = 0.0056), but not BRO-negative isolates ( P = 0.1333). We provide the first evidence that the presence of BRO-1 in M. catarrhalis is associated with reduced susceptibility to clarithromycin and β-lactam antibiotics, as well as serum non-sensitive (intermediate and resistant).</description><subject>Anti-Infective Agents - pharmacology</subject><subject>beta-Lactamases - analysis</subject><subject>beta-Lactamases - biosynthesis</subject><subject>Hematology, Oncology and Palliative Medicine</subject><subject>Humans</subject><subject>Macrolide</subject><subject>Microbial Sensitivity Tests - methods</subject><subject>Moraxella (Branhamella) catarrhalis - drug effects</subject><subject>Moraxella (Branhamella) catarrhalis - enzymology</subject><subject>Moraxella (Branhamella) catarrhalis - isolation & purification</subject><subject>Moraxella catarrhalis</subject><subject>Moraxellaceae Infections - drug therapy</subject><subject>Moraxellaceae Infections - microbiology</subject><subject>Respiratory Tract Infections - drug therapy</subject><subject>Respiratory Tract Infections - microbiology</subject><subject>Serum resistance</subject><subject>β-Lactam</subject><subject>β-Lactamase</subject><issn>1341-321X</issn><issn>1437-7780</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc2q1TAUhYso3h99AQeSoZPW_DVtQQS96FW4ckGv4CzsprucXftzTFLxzH0iH8RnMvVcHTgwk4Sw1mKvb2fZI8ELwYV5OhQDgSskF6rgpuBc3clOhVZVXlU1v5veSotcSfHpJDsLYeBcVGVd389OpNZGamFOs-83O2S9xy8rzu7Alp69fH_Nfv7IR3ARJgjIYO4YxcA8jhBpmcOO9iwu6T_SRM4vLcHIwhoc7iO1NFI8_DYF9OuUbIFChNkho5m9Wzx8w3EE5iCC9zsYKTzI7vUwBnx4e59nH1-_url4k19dX769eHGVu5KXMTed4rISphE1d64HLQDbDntlJFdV3ZqygtbVsgHoJHLodNmbRkrZo9NNI9V59uSYu_dLKhyinShNnaaZcVmDFUkldG0qk6TyKE39QvDY272nCfzBCm43-nawG3270bfc2EQ_mR7f5q_thN1fyx_cSfDsKMDU8iuht8FRAo8deXTRdgv9P__5P3Y30kwOxs94wDAsq58TPytskJbbD9v-t_ULxdPRWv0CtyCuGQ</recordid><startdate>20140101</startdate><enddate>20140101</enddate><creator>Saito, Ryoichi</creator><creator>Nonaka, Shotaro</creator><creator>Fujinami, Yuji</creator><creator>Matsuoka, Shiho</creator><creator>Nakajima, Shinichi</creator><creator>Nishiyama, Hiroyuki</creator><creator>Okamura, Noboru</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20140101</creationdate><title>The frequency of BRO β-lactamase and its relationship to antimicrobial susceptibility and serum resistance in Moraxella catarrhalis</title><author>Saito, Ryoichi ; Nonaka, Shotaro ; Fujinami, Yuji ; Matsuoka, Shiho ; Nakajima, Shinichi ; Nishiyama, Hiroyuki ; Okamura, Noboru</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c505t-6d3027169180ccfa41aebdef3620378b657abc829aad2e0ad45f69222fec49923</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Anti-Infective Agents - pharmacology</topic><topic>beta-Lactamases - analysis</topic><topic>beta-Lactamases - biosynthesis</topic><topic>Hematology, Oncology and Palliative Medicine</topic><topic>Humans</topic><topic>Macrolide</topic><topic>Microbial Sensitivity Tests - methods</topic><topic>Moraxella (Branhamella) catarrhalis - drug effects</topic><topic>Moraxella (Branhamella) catarrhalis - enzymology</topic><topic>Moraxella (Branhamella) catarrhalis - isolation & purification</topic><topic>Moraxella catarrhalis</topic><topic>Moraxellaceae Infections - drug therapy</topic><topic>Moraxellaceae Infections - microbiology</topic><topic>Respiratory Tract Infections - drug therapy</topic><topic>Respiratory Tract Infections - microbiology</topic><topic>Serum resistance</topic><topic>β-Lactam</topic><topic>β-Lactamase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Saito, Ryoichi</creatorcontrib><creatorcontrib>Nonaka, Shotaro</creatorcontrib><creatorcontrib>Fujinami, Yuji</creatorcontrib><creatorcontrib>Matsuoka, Shiho</creatorcontrib><creatorcontrib>Nakajima, Shinichi</creatorcontrib><creatorcontrib>Nishiyama, Hiroyuki</creatorcontrib><creatorcontrib>Okamura, Noboru</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Saito, Ryoichi</au><au>Nonaka, Shotaro</au><au>Fujinami, Yuji</au><au>Matsuoka, Shiho</au><au>Nakajima, Shinichi</au><au>Nishiyama, Hiroyuki</au><au>Okamura, Noboru</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The frequency of BRO β-lactamase and its relationship to antimicrobial susceptibility and serum resistance in Moraxella catarrhalis</atitle><jtitle>Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy</jtitle><addtitle>J Infect Chemother</addtitle><date>2014-01-01</date><risdate>2014</risdate><volume>20</volume><issue>1</issue><spage>6</spage><epage>8</epage><pages>6-8</pages><issn>1341-321X</issn><eissn>1437-7780</eissn><abstract>Abstract We investigated the frequency of BRO β-lactamase and its relationship to antibiotic susceptibility profiles and serum susceptibility. Moraxella catarrhalis clinical isolates ( n = 197) were collected from patients with respiratory tract infections in Tokyo between November 2004 and April 2005. Phenotypic and genotypic detection of β-lactamases was performed. The MICs of 6 antibiotics were determined by Etest, and the serum bactericidal assay was conducted by using the culture-and-spot test. Nearly all (192; 97%) of the clinical isolates were β-lactamase producers; of these, 182 (95%) were bro-1 and 10 (5%) were bro-2 positive. MIC50 , MIC90 , and geometric mean MICs of penicillin, amoxicillin, cefixime, and clarithromycin for BRO-1 isolates were significantly higher than for BRO-2 isolates. The frequency of intermediate and full serum resistance was significantly higher in BRO-1 isolates than in BRO-2 isolates ( P = 0.0056), but not BRO-negative isolates ( P = 0.1333). We provide the first evidence that the presence of BRO-1 in M. catarrhalis is associated with reduced susceptibility to clarithromycin and β-lactam antibiotics, as well as serum non-sensitive (intermediate and resistant).</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>24462416</pmid><doi>10.1016/j.jiac.2013.06.003</doi><tpages>3</tpages></addata></record> |
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| subjects | Anti-Infective Agents - pharmacology beta-Lactamases - analysis beta-Lactamases - biosynthesis Hematology, Oncology and Palliative Medicine Humans Macrolide Microbial Sensitivity Tests - methods Moraxella (Branhamella) catarrhalis - drug effects Moraxella (Branhamella) catarrhalis - enzymology Moraxella (Branhamella) catarrhalis - isolation & purification Moraxella catarrhalis Moraxellaceae Infections - drug therapy Moraxellaceae Infections - microbiology Respiratory Tract Infections - drug therapy Respiratory Tract Infections - microbiology Serum resistance β-Lactam β-Lactamase |
| title | The frequency of BRO β-lactamase and its relationship to antimicrobial susceptibility and serum resistance in Moraxella catarrhalis |
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