Atractylodes macrocephala Koidz promotes intestinal epithelial restitution via the polyamine—Voltage-gated K+ channel pathway
Atractylodes macrocephala Koidz (AMK) has been used widely as a digestive and tonic in traditional Chinese medicine. AMK has shown noteworthy promoting effect on intestinal epithelial cell migration, which might represent a promising candidate for the treatment of intestinal mucosa injury. The aim o...
Gespeichert in:
| Veröffentlicht in: | Journal of ethnopharmacology 2014-02, Vol.152 (1), p.163-172 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 172 |
|---|---|
| container_issue | 1 |
| container_start_page | 163 |
| container_title | Journal of ethnopharmacology |
| container_volume | 152 |
| creator | Song, Hou-Pan Li, Ru-Liu Chen, Xu Wang, Yi-Yu Cai, Jia-Zhong Liu, Jia Chen, Wei-Wen |
| description | Atractylodes macrocephala Koidz (AMK) has been used widely as a digestive and tonic in traditional Chinese medicine. AMK has shown noteworthy promoting effect on intestinal epithelial cell migration, which might represent a promising candidate for the treatment of intestinal mucosa injury. The aim of this study was to investigate the efficacy of AMK on intestinal mucosal restitution and the underlying mechanisms via IEC-6 cell migration model.
A wounding model of IEC-6 cells was induced by a single-edge razor blade along the diameter of six-well polystyrene plates. The cells were grown in control cultures and in cultures containing spermidine (5μmol/L, SPD, reference drug), alpha-difluoromethylornithine (2.5mmol/L, DFMO, polyamine inhibitor), AMK (50, 100, and 200μg/mL), DFMO plus SPD and DFMO plus AMK for 24h. The membrane potential (MP) and cytosolic free Ca2+ concentration ([Ca2+]cyt) were detected by flow cytometry, and polyamines content was determined via high-performance liquid chromatography (HPLC). The expression of Kv1.1 mRNA and protein levels were assessed by RT-qPCR and Western blot analysis, respectively. Cell migration assay was carried out using the Image-Pro Plus software. All of these indexes were used to evaluate the effectiveness of AMK.
(1) Treatment with AMK caused significant increases in cellular polyamines content, membrane hyperpolarization, an elevation of [Ca2+]cyt and an acceleration of cell migration in IEC-6 cells, as compared to control group. (2) AMK not only reversed the inhibitory effects of DFMO on the polyamines content, MP, and [Ca2+]cyt but also restored IEC-6 cell migration to control levels. (3) The Kv1.1 mRNA and protein expression were significantly increased by AMK treatment in control and polyamine-deficient IEC-6 cells.
The results of our current studies revealed that treatment with AMK significantly stimulates the migration of intestinal epithelial cells through polyamine-Kv1.1 channel signaling pathway, which could promote the healing of intestinal injury. These results suggest the potential usefulness of AMK to cure intestinal disorders characterized by injury and ineffective repair of the intestinal mucosa.
[Display omitted] |
| doi_str_mv | 10.1016/j.jep.2013.12.049 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1499143635</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0378874113009318</els_id><sourcerecordid>1499143635</sourcerecordid><originalsourceid>FETCH-LOGICAL-c353t-4f7e771889b3ee847102a3c7ada17441c52c00a17d5ed7d88b7fe145afecf37a3</originalsourceid><addsrcrecordid>eNp9kMFuEzEQhi0EoiHwAFyQj0hoF3vtjR1xqioKqJW4AFdrYs82jrzrxXZapRd4CJ6QJ8FRCkcu9mj8zz_-P0JectZyxldvd-0O57ZjXLS8a5lcPyILrlXXqF6Jx2TBhNKNVpKfkWc57xhjikv2lJx1UnKlV2pBfpyXBLYcQnSY6Qg2RYvzFgLQq-jdPZ1THGOpb36qZ_ETBIqzL1sMvpbp2Cv74uNEbz3Q2qdzDAcY_YS_f_76FkOBG2xuoKCjV2-o3cI0YaAzlO0dHJ6TJwOEjC8e7iX5evn-y8XH5vrzh08X59eNFb0ojRwUKsW1Xm8EopaKsw6EVeCAqxrG9p1lrNauR6ec1hs1IJc9DGgHoUAsyeuTb83zfV8_bUafLYYAE8Z9Nlyu11yKVd22JPwkrSxyTjiYOfkR0sFwZo7czc5U7ubI3fDOVO515tWD_X4zovs38Rd0Fbw7CbCGvPWYTLYeJ4vOJ7TFuOj_Y_8HIgWXow</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1499143635</pqid></control><display><type>article</type><title>Atractylodes macrocephala Koidz promotes intestinal epithelial restitution via the polyamine—Voltage-gated K+ channel pathway</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Song, Hou-Pan ; Li, Ru-Liu ; Chen, Xu ; Wang, Yi-Yu ; Cai, Jia-Zhong ; Liu, Jia ; Chen, Wei-Wen</creator><creatorcontrib>Song, Hou-Pan ; Li, Ru-Liu ; Chen, Xu ; Wang, Yi-Yu ; Cai, Jia-Zhong ; Liu, Jia ; Chen, Wei-Wen</creatorcontrib><description>Atractylodes macrocephala Koidz (AMK) has been used widely as a digestive and tonic in traditional Chinese medicine. AMK has shown noteworthy promoting effect on intestinal epithelial cell migration, which might represent a promising candidate for the treatment of intestinal mucosa injury. The aim of this study was to investigate the efficacy of AMK on intestinal mucosal restitution and the underlying mechanisms via IEC-6 cell migration model.
A wounding model of IEC-6 cells was induced by a single-edge razor blade along the diameter of six-well polystyrene plates. The cells were grown in control cultures and in cultures containing spermidine (5μmol/L, SPD, reference drug), alpha-difluoromethylornithine (2.5mmol/L, DFMO, polyamine inhibitor), AMK (50, 100, and 200μg/mL), DFMO plus SPD and DFMO plus AMK for 24h. The membrane potential (MP) and cytosolic free Ca2+ concentration ([Ca2+]cyt) were detected by flow cytometry, and polyamines content was determined via high-performance liquid chromatography (HPLC). The expression of Kv1.1 mRNA and protein levels were assessed by RT-qPCR and Western blot analysis, respectively. Cell migration assay was carried out using the Image-Pro Plus software. All of these indexes were used to evaluate the effectiveness of AMK.
(1) Treatment with AMK caused significant increases in cellular polyamines content, membrane hyperpolarization, an elevation of [Ca2+]cyt and an acceleration of cell migration in IEC-6 cells, as compared to control group. (2) AMK not only reversed the inhibitory effects of DFMO on the polyamines content, MP, and [Ca2+]cyt but also restored IEC-6 cell migration to control levels. (3) The Kv1.1 mRNA and protein expression were significantly increased by AMK treatment in control and polyamine-deficient IEC-6 cells.
The results of our current studies revealed that treatment with AMK significantly stimulates the migration of intestinal epithelial cells through polyamine-Kv1.1 channel signaling pathway, which could promote the healing of intestinal injury. These results suggest the potential usefulness of AMK to cure intestinal disorders characterized by injury and ineffective repair of the intestinal mucosa.
[Display omitted]</description><identifier>ISSN: 0378-8741</identifier><identifier>EISSN: 1872-7573</identifier><identifier>DOI: 10.1016/j.jep.2013.12.049</identifier><identifier>PMID: 24417867</identifier><language>eng</language><publisher>Ireland: Elsevier Ireland Ltd</publisher><subject>Animals ; Atractylodes - chemistry ; Atractylodes macrocephala Koidz ; Calcium - metabolism ; Cell Line ; Cell migration ; Cell Movement - drug effects ; Chromatography, High Pressure Liquid ; Dose-Response Relationship, Drug ; Flow Cytometry ; Intestinal epithelial cells ; Intestinal Mucosa - cytology ; Intestinal Mucosa - drug effects ; Intestinal Mucosa - pathology ; Intracellular calcium ; Kv1.1 Potassium Channel - genetics ; Kv1.1 Potassium Channel - metabolism ; Membrane potential ; Membrane Potentials - drug effects ; Plant Extracts - administration & dosage ; Plant Extracts - pharmacology ; Polyamines ; Polyamines - metabolism ; Rats ; RNA, Messenger - metabolism ; Signal Transduction - drug effects</subject><ispartof>Journal of ethnopharmacology, 2014-02, Vol.152 (1), p.163-172</ispartof><rights>2014</rights><rights>Crown Copyright © 2014. Published by Elsevier Ireland Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c353t-4f7e771889b3ee847102a3c7ada17441c52c00a17d5ed7d88b7fe145afecf37a3</citedby><cites>FETCH-LOGICAL-c353t-4f7e771889b3ee847102a3c7ada17441c52c00a17d5ed7d88b7fe145afecf37a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jep.2013.12.049$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24417867$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Song, Hou-Pan</creatorcontrib><creatorcontrib>Li, Ru-Liu</creatorcontrib><creatorcontrib>Chen, Xu</creatorcontrib><creatorcontrib>Wang, Yi-Yu</creatorcontrib><creatorcontrib>Cai, Jia-Zhong</creatorcontrib><creatorcontrib>Liu, Jia</creatorcontrib><creatorcontrib>Chen, Wei-Wen</creatorcontrib><title>Atractylodes macrocephala Koidz promotes intestinal epithelial restitution via the polyamine—Voltage-gated K+ channel pathway</title><title>Journal of ethnopharmacology</title><addtitle>J Ethnopharmacol</addtitle><description>Atractylodes macrocephala Koidz (AMK) has been used widely as a digestive and tonic in traditional Chinese medicine. AMK has shown noteworthy promoting effect on intestinal epithelial cell migration, which might represent a promising candidate for the treatment of intestinal mucosa injury. The aim of this study was to investigate the efficacy of AMK on intestinal mucosal restitution and the underlying mechanisms via IEC-6 cell migration model.
A wounding model of IEC-6 cells was induced by a single-edge razor blade along the diameter of six-well polystyrene plates. The cells were grown in control cultures and in cultures containing spermidine (5μmol/L, SPD, reference drug), alpha-difluoromethylornithine (2.5mmol/L, DFMO, polyamine inhibitor), AMK (50, 100, and 200μg/mL), DFMO plus SPD and DFMO plus AMK for 24h. The membrane potential (MP) and cytosolic free Ca2+ concentration ([Ca2+]cyt) were detected by flow cytometry, and polyamines content was determined via high-performance liquid chromatography (HPLC). The expression of Kv1.1 mRNA and protein levels were assessed by RT-qPCR and Western blot analysis, respectively. Cell migration assay was carried out using the Image-Pro Plus software. All of these indexes were used to evaluate the effectiveness of AMK.
(1) Treatment with AMK caused significant increases in cellular polyamines content, membrane hyperpolarization, an elevation of [Ca2+]cyt and an acceleration of cell migration in IEC-6 cells, as compared to control group. (2) AMK not only reversed the inhibitory effects of DFMO on the polyamines content, MP, and [Ca2+]cyt but also restored IEC-6 cell migration to control levels. (3) The Kv1.1 mRNA and protein expression were significantly increased by AMK treatment in control and polyamine-deficient IEC-6 cells.
The results of our current studies revealed that treatment with AMK significantly stimulates the migration of intestinal epithelial cells through polyamine-Kv1.1 channel signaling pathway, which could promote the healing of intestinal injury. These results suggest the potential usefulness of AMK to cure intestinal disorders characterized by injury and ineffective repair of the intestinal mucosa.
[Display omitted]</description><subject>Animals</subject><subject>Atractylodes - chemistry</subject><subject>Atractylodes macrocephala Koidz</subject><subject>Calcium - metabolism</subject><subject>Cell Line</subject><subject>Cell migration</subject><subject>Cell Movement - drug effects</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Dose-Response Relationship, Drug</subject><subject>Flow Cytometry</subject><subject>Intestinal epithelial cells</subject><subject>Intestinal Mucosa - cytology</subject><subject>Intestinal Mucosa - drug effects</subject><subject>Intestinal Mucosa - pathology</subject><subject>Intracellular calcium</subject><subject>Kv1.1 Potassium Channel - genetics</subject><subject>Kv1.1 Potassium Channel - metabolism</subject><subject>Membrane potential</subject><subject>Membrane Potentials - drug effects</subject><subject>Plant Extracts - administration & dosage</subject><subject>Plant Extracts - pharmacology</subject><subject>Polyamines</subject><subject>Polyamines - metabolism</subject><subject>Rats</subject><subject>RNA, Messenger - metabolism</subject><subject>Signal Transduction - drug effects</subject><issn>0378-8741</issn><issn>1872-7573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMFuEzEQhi0EoiHwAFyQj0hoF3vtjR1xqioKqJW4AFdrYs82jrzrxXZapRd4CJ6QJ8FRCkcu9mj8zz_-P0JectZyxldvd-0O57ZjXLS8a5lcPyILrlXXqF6Jx2TBhNKNVpKfkWc57xhjikv2lJx1UnKlV2pBfpyXBLYcQnSY6Qg2RYvzFgLQq-jdPZ1THGOpb36qZ_ETBIqzL1sMvpbp2Cv74uNEbz3Q2qdzDAcY_YS_f_76FkOBG2xuoKCjV2-o3cI0YaAzlO0dHJ6TJwOEjC8e7iX5evn-y8XH5vrzh08X59eNFb0ojRwUKsW1Xm8EopaKsw6EVeCAqxrG9p1lrNauR6ec1hs1IJc9DGgHoUAsyeuTb83zfV8_bUafLYYAE8Z9Nlyu11yKVd22JPwkrSxyTjiYOfkR0sFwZo7czc5U7ubI3fDOVO515tWD_X4zovs38Rd0Fbw7CbCGvPWYTLYeJ4vOJ7TFuOj_Y_8HIgWXow</recordid><startdate>20140227</startdate><enddate>20140227</enddate><creator>Song, Hou-Pan</creator><creator>Li, Ru-Liu</creator><creator>Chen, Xu</creator><creator>Wang, Yi-Yu</creator><creator>Cai, Jia-Zhong</creator><creator>Liu, Jia</creator><creator>Chen, Wei-Wen</creator><general>Elsevier Ireland Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20140227</creationdate><title>Atractylodes macrocephala Koidz promotes intestinal epithelial restitution via the polyamine—Voltage-gated K+ channel pathway</title><author>Song, Hou-Pan ; Li, Ru-Liu ; Chen, Xu ; Wang, Yi-Yu ; Cai, Jia-Zhong ; Liu, Jia ; Chen, Wei-Wen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c353t-4f7e771889b3ee847102a3c7ada17441c52c00a17d5ed7d88b7fe145afecf37a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Atractylodes - chemistry</topic><topic>Atractylodes macrocephala Koidz</topic><topic>Calcium - metabolism</topic><topic>Cell Line</topic><topic>Cell migration</topic><topic>Cell Movement - drug effects</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Dose-Response Relationship, Drug</topic><topic>Flow Cytometry</topic><topic>Intestinal epithelial cells</topic><topic>Intestinal Mucosa - cytology</topic><topic>Intestinal Mucosa - drug effects</topic><topic>Intestinal Mucosa - pathology</topic><topic>Intracellular calcium</topic><topic>Kv1.1 Potassium Channel - genetics</topic><topic>Kv1.1 Potassium Channel - metabolism</topic><topic>Membrane potential</topic><topic>Membrane Potentials - drug effects</topic><topic>Plant Extracts - administration & dosage</topic><topic>Plant Extracts - pharmacology</topic><topic>Polyamines</topic><topic>Polyamines - metabolism</topic><topic>Rats</topic><topic>RNA, Messenger - metabolism</topic><topic>Signal Transduction - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Song, Hou-Pan</creatorcontrib><creatorcontrib>Li, Ru-Liu</creatorcontrib><creatorcontrib>Chen, Xu</creatorcontrib><creatorcontrib>Wang, Yi-Yu</creatorcontrib><creatorcontrib>Cai, Jia-Zhong</creatorcontrib><creatorcontrib>Liu, Jia</creatorcontrib><creatorcontrib>Chen, Wei-Wen</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of ethnopharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Song, Hou-Pan</au><au>Li, Ru-Liu</au><au>Chen, Xu</au><au>Wang, Yi-Yu</au><au>Cai, Jia-Zhong</au><au>Liu, Jia</au><au>Chen, Wei-Wen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Atractylodes macrocephala Koidz promotes intestinal epithelial restitution via the polyamine—Voltage-gated K+ channel pathway</atitle><jtitle>Journal of ethnopharmacology</jtitle><addtitle>J Ethnopharmacol</addtitle><date>2014-02-27</date><risdate>2014</risdate><volume>152</volume><issue>1</issue><spage>163</spage><epage>172</epage><pages>163-172</pages><issn>0378-8741</issn><eissn>1872-7573</eissn><abstract>Atractylodes macrocephala Koidz (AMK) has been used widely as a digestive and tonic in traditional Chinese medicine. AMK has shown noteworthy promoting effect on intestinal epithelial cell migration, which might represent a promising candidate for the treatment of intestinal mucosa injury. The aim of this study was to investigate the efficacy of AMK on intestinal mucosal restitution and the underlying mechanisms via IEC-6 cell migration model.
A wounding model of IEC-6 cells was induced by a single-edge razor blade along the diameter of six-well polystyrene plates. The cells were grown in control cultures and in cultures containing spermidine (5μmol/L, SPD, reference drug), alpha-difluoromethylornithine (2.5mmol/L, DFMO, polyamine inhibitor), AMK (50, 100, and 200μg/mL), DFMO plus SPD and DFMO plus AMK for 24h. The membrane potential (MP) and cytosolic free Ca2+ concentration ([Ca2+]cyt) were detected by flow cytometry, and polyamines content was determined via high-performance liquid chromatography (HPLC). The expression of Kv1.1 mRNA and protein levels were assessed by RT-qPCR and Western blot analysis, respectively. Cell migration assay was carried out using the Image-Pro Plus software. All of these indexes were used to evaluate the effectiveness of AMK.
(1) Treatment with AMK caused significant increases in cellular polyamines content, membrane hyperpolarization, an elevation of [Ca2+]cyt and an acceleration of cell migration in IEC-6 cells, as compared to control group. (2) AMK not only reversed the inhibitory effects of DFMO on the polyamines content, MP, and [Ca2+]cyt but also restored IEC-6 cell migration to control levels. (3) The Kv1.1 mRNA and protein expression were significantly increased by AMK treatment in control and polyamine-deficient IEC-6 cells.
The results of our current studies revealed that treatment with AMK significantly stimulates the migration of intestinal epithelial cells through polyamine-Kv1.1 channel signaling pathway, which could promote the healing of intestinal injury. These results suggest the potential usefulness of AMK to cure intestinal disorders characterized by injury and ineffective repair of the intestinal mucosa.
[Display omitted]</abstract><cop>Ireland</cop><pub>Elsevier Ireland Ltd</pub><pmid>24417867</pmid><doi>10.1016/j.jep.2013.12.049</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-8741 |
| ispartof | Journal of ethnopharmacology, 2014-02, Vol.152 (1), p.163-172 |
| issn | 0378-8741 1872-7573 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1499143635 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals Atractylodes - chemistry Atractylodes macrocephala Koidz Calcium - metabolism Cell Line Cell migration Cell Movement - drug effects Chromatography, High Pressure Liquid Dose-Response Relationship, Drug Flow Cytometry Intestinal epithelial cells Intestinal Mucosa - cytology Intestinal Mucosa - drug effects Intestinal Mucosa - pathology Intracellular calcium Kv1.1 Potassium Channel - genetics Kv1.1 Potassium Channel - metabolism Membrane potential Membrane Potentials - drug effects Plant Extracts - administration & dosage Plant Extracts - pharmacology Polyamines Polyamines - metabolism Rats RNA, Messenger - metabolism Signal Transduction - drug effects |
| title | Atractylodes macrocephala Koidz promotes intestinal epithelial restitution via the polyamine—Voltage-gated K+ channel pathway |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-30T21%3A08%3A55IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Atractylodes%20macrocephala%20Koidz%20promotes%20intestinal%20epithelial%20restitution%20via%20the%20polyamine%E2%80%94Voltage-gated%20K+%20channel%20pathway&rft.jtitle=Journal%20of%20ethnopharmacology&rft.au=Song,%20Hou-Pan&rft.date=2014-02-27&rft.volume=152&rft.issue=1&rft.spage=163&rft.epage=172&rft.pages=163-172&rft.issn=0378-8741&rft.eissn=1872-7573&rft_id=info:doi/10.1016/j.jep.2013.12.049&rft_dat=%3Cproquest_cross%3E1499143635%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1499143635&rft_id=info:pmid/24417867&rft_els_id=S0378874113009318&rfr_iscdi=true |