Mechanism of Saccharomyces cerevisiae yeast cell death induced by heat shock. Effect of cycloheximide on thermotolerance
The mechanism of yeast cell death induced by heat shock was found to be dependent on the intensity of heat exposure. Moderate (45°C) heat shock strongly increased the generation of reactive oxygen species (ROS) and cell death. Pretreatment with cycloheximide (at 30°C) suppressed cell death, but prod...
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| Veröffentlicht in: | Biochemistry (Moscow) 2014, Vol.79 (1), p.16-24 |
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| creator | Rikhvanov, E. G. Fedoseeva, I. V. Varakina, N. N. Rusaleva, T. M. Fedyaeva, A. V. |
| description | The mechanism of yeast cell death induced by heat shock was found to be dependent on the intensity of heat exposure. Moderate (45°C) heat shock strongly increased the generation of reactive oxygen species (ROS) and cell death. Pretreatment with cycloheximide (at 30°C) suppressed cell death, but produced no effect on ROS production. The protective effect was absent if cycloheximide was added immediately before heat exposure and the cells were incubated with the drug during the heat treatment and recovery period. The rate of ROS production and protective effect of cycloheximide on viability were significantly decreased in the case of severe (50°C) heat shock. Treatment with cycloheximide at 39°C inhibited the induction of Hsp104 synthesis and suppressed the development of induced thermotolerance to severe shock (50°C), but it had no effect on induced thermotolerance to moderate (45°C) heat shock. At the same time, Hsp104 effectively protected cells from death independently of the intensity of heat exposure. These data indicate that moderate heat shock induced programmed cell death in the yeast cells, and cycloheximide suppressed this process by inhibiting general synthesis of proteins. |
| doi_str_mv | 10.1134/S0006297914010039 |
| format | Article |
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Effect of cycloheximide on thermotolerance</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Rikhvanov, E. G. ; Fedoseeva, I. V. ; Varakina, N. N. ; Rusaleva, T. M. ; Fedyaeva, A. V.</creator><creatorcontrib>Rikhvanov, E. G. ; Fedoseeva, I. V. ; Varakina, N. N. ; Rusaleva, T. M. ; Fedyaeva, A. V.</creatorcontrib><description>The mechanism of yeast cell death induced by heat shock was found to be dependent on the intensity of heat exposure. Moderate (45°C) heat shock strongly increased the generation of reactive oxygen species (ROS) and cell death. Pretreatment with cycloheximide (at 30°C) suppressed cell death, but produced no effect on ROS production. The protective effect was absent if cycloheximide was added immediately before heat exposure and the cells were incubated with the drug during the heat treatment and recovery period. The rate of ROS production and protective effect of cycloheximide on viability were significantly decreased in the case of severe (50°C) heat shock. Treatment with cycloheximide at 39°C inhibited the induction of Hsp104 synthesis and suppressed the development of induced thermotolerance to severe shock (50°C), but it had no effect on induced thermotolerance to moderate (45°C) heat shock. At the same time, Hsp104 effectively protected cells from death independently of the intensity of heat exposure. These data indicate that moderate heat shock induced programmed cell death in the yeast cells, and cycloheximide suppressed this process by inhibiting general synthesis of proteins.</description><identifier>ISSN: 0006-2979</identifier><identifier>EISSN: 1608-3040</identifier><identifier>DOI: 10.1134/S0006297914010039</identifier><identifier>PMID: 24512659</identifier><language>eng</language><publisher>Boston: Springer US</publisher><subject>Apoptosis ; Biochemistry ; Biomedical and Life Sciences ; Biomedicine ; Bioorganic Chemistry ; Brewer's yeast ; Cell death ; Cycloheximide - chemistry ; Cycloheximide - pharmacology ; Heat ; Heat shock proteins ; Heat treatment ; Heat-Shock Proteins - chemistry ; Heat-Shock Proteins - metabolism ; Life Sciences ; Microbiology ; Mortality ; Physiological aspects ; Protein synthesis ; Protein Synthesis Inhibitors - chemistry ; Protein Synthesis Inhibitors - pharmacology ; Reactive Oxygen Species - metabolism ; Saccharomyces cerevisiae - drug effects ; Saccharomyces cerevisiae - metabolism ; Saccharomyces cerevisiae Proteins - chemistry ; Saccharomyces cerevisiae Proteins - metabolism ; Temperature ; Yeast ; Yeasts</subject><ispartof>Biochemistry (Moscow), 2014, Vol.79 (1), p.16-24</ispartof><rights>Pleiades Publishing, Ltd. 2014</rights><rights>COPYRIGHT 2014 Springer</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c439t-e544b3a6d441a46990ef0d07bba7371e6cb5254befa417988d0c84ddc111a3493</citedby><cites>FETCH-LOGICAL-c439t-e544b3a6d441a46990ef0d07bba7371e6cb5254befa417988d0c84ddc111a3493</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1134/S0006297914010039$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1134/S0006297914010039$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24512659$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rikhvanov, E. G.</creatorcontrib><creatorcontrib>Fedoseeva, I. V.</creatorcontrib><creatorcontrib>Varakina, N. N.</creatorcontrib><creatorcontrib>Rusaleva, T. M.</creatorcontrib><creatorcontrib>Fedyaeva, A. V.</creatorcontrib><title>Mechanism of Saccharomyces cerevisiae yeast cell death induced by heat shock. Effect of cycloheximide on thermotolerance</title><title>Biochemistry (Moscow)</title><addtitle>Biochemistry Moscow</addtitle><addtitle>Biochemistry (Mosc)</addtitle><description>The mechanism of yeast cell death induced by heat shock was found to be dependent on the intensity of heat exposure. Moderate (45°C) heat shock strongly increased the generation of reactive oxygen species (ROS) and cell death. Pretreatment with cycloheximide (at 30°C) suppressed cell death, but produced no effect on ROS production. The protective effect was absent if cycloheximide was added immediately before heat exposure and the cells were incubated with the drug during the heat treatment and recovery period. The rate of ROS production and protective effect of cycloheximide on viability were significantly decreased in the case of severe (50°C) heat shock. Treatment with cycloheximide at 39°C inhibited the induction of Hsp104 synthesis and suppressed the development of induced thermotolerance to severe shock (50°C), but it had no effect on induced thermotolerance to moderate (45°C) heat shock. At the same time, Hsp104 effectively protected cells from death independently of the intensity of heat exposure. These data indicate that moderate heat shock induced programmed cell death in the yeast cells, and cycloheximide suppressed this process by inhibiting general synthesis of proteins.</description><subject>Apoptosis</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Bioorganic Chemistry</subject><subject>Brewer's yeast</subject><subject>Cell death</subject><subject>Cycloheximide - chemistry</subject><subject>Cycloheximide - pharmacology</subject><subject>Heat</subject><subject>Heat shock proteins</subject><subject>Heat treatment</subject><subject>Heat-Shock Proteins - chemistry</subject><subject>Heat-Shock Proteins - metabolism</subject><subject>Life Sciences</subject><subject>Microbiology</subject><subject>Mortality</subject><subject>Physiological aspects</subject><subject>Protein synthesis</subject><subject>Protein Synthesis Inhibitors - chemistry</subject><subject>Protein Synthesis Inhibitors - pharmacology</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Saccharomyces cerevisiae - drug effects</subject><subject>Saccharomyces cerevisiae - metabolism</subject><subject>Saccharomyces cerevisiae Proteins - chemistry</subject><subject>Saccharomyces cerevisiae Proteins - metabolism</subject><subject>Temperature</subject><subject>Yeast</subject><subject>Yeasts</subject><issn>0006-2979</issn><issn>1608-3040</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kUtv1TAQhS0EopfCD2CDLLFhk8s4dh5eVlUpSEUsCuvIsceNS2IXO6maf19Ht7xBXlgz852jGR1CXjLYM8bF20sAqEvZSCaAAXD5iOxYDW3BQcBjstvGxTY_Is9Sus5lCZI_JUelqFhZV3JH7j6iHpR3aaLB0kulcxXDtGpMVGPEW5ecQrqiSnNujCM1qOaBOm8WjYb2Kx1yg6Yh6K97emYt6nmz0qsew4B3bnIGafB0HjBOYQ4jRuU1PidPrBoTvnj4j8mXd2efT98XF5_OP5yeXBRacDkXWAnRc1UbIZgStZSAFgw0fa8a3jCsdV-VlejRKsEa2bYGdCuM0YwxxYXkx-TNwfcmhm8LprmbXNoOUR7DkjompGS8hLrN6Os_0OuwRJ-326hSCKib6id1pUbsnLdhjkpvpt0JzwtuWfBM7f9B5Wdwcjp4tC73fxOwg0DHkFJE291EN6m4dgy6Le3ur7Sz5tXDwks_ofmh-B5vBsoDkPLIX2H85aL_ut4DOM-yVQ</recordid><startdate>2014</startdate><enddate>2014</enddate><creator>Rikhvanov, E. 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V.</creator><general>Springer US</general><general>Springer</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>2014</creationdate><title>Mechanism of Saccharomyces cerevisiae yeast cell death induced by heat shock. Effect of cycloheximide on thermotolerance</title><author>Rikhvanov, E. G. ; Fedoseeva, I. V. ; Varakina, N. N. ; Rusaleva, T. M. ; Fedyaeva, A. 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G.</au><au>Fedoseeva, I. V.</au><au>Varakina, N. N.</au><au>Rusaleva, T. M.</au><au>Fedyaeva, A. V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mechanism of Saccharomyces cerevisiae yeast cell death induced by heat shock. Effect of cycloheximide on thermotolerance</atitle><jtitle>Biochemistry (Moscow)</jtitle><stitle>Biochemistry Moscow</stitle><addtitle>Biochemistry (Mosc)</addtitle><date>2014</date><risdate>2014</risdate><volume>79</volume><issue>1</issue><spage>16</spage><epage>24</epage><pages>16-24</pages><issn>0006-2979</issn><eissn>1608-3040</eissn><abstract>The mechanism of yeast cell death induced by heat shock was found to be dependent on the intensity of heat exposure. Moderate (45°C) heat shock strongly increased the generation of reactive oxygen species (ROS) and cell death. Pretreatment with cycloheximide (at 30°C) suppressed cell death, but produced no effect on ROS production. The protective effect was absent if cycloheximide was added immediately before heat exposure and the cells were incubated with the drug during the heat treatment and recovery period. The rate of ROS production and protective effect of cycloheximide on viability were significantly decreased in the case of severe (50°C) heat shock. Treatment with cycloheximide at 39°C inhibited the induction of Hsp104 synthesis and suppressed the development of induced thermotolerance to severe shock (50°C), but it had no effect on induced thermotolerance to moderate (45°C) heat shock. At the same time, Hsp104 effectively protected cells from death independently of the intensity of heat exposure. These data indicate that moderate heat shock induced programmed cell death in the yeast cells, and cycloheximide suppressed this process by inhibiting general synthesis of proteins.</abstract><cop>Boston</cop><pub>Springer US</pub><pmid>24512659</pmid><doi>10.1134/S0006297914010039</doi><tpages>9</tpages></addata></record> |
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| subjects | Apoptosis Biochemistry Biomedical and Life Sciences Biomedicine Bioorganic Chemistry Brewer's yeast Cell death Cycloheximide - chemistry Cycloheximide - pharmacology Heat Heat shock proteins Heat treatment Heat-Shock Proteins - chemistry Heat-Shock Proteins - metabolism Life Sciences Microbiology Mortality Physiological aspects Protein synthesis Protein Synthesis Inhibitors - chemistry Protein Synthesis Inhibitors - pharmacology Reactive Oxygen Species - metabolism Saccharomyces cerevisiae - drug effects Saccharomyces cerevisiae - metabolism Saccharomyces cerevisiae Proteins - chemistry Saccharomyces cerevisiae Proteins - metabolism Temperature Yeast Yeasts |
| title | Mechanism of Saccharomyces cerevisiae yeast cell death induced by heat shock. Effect of cycloheximide on thermotolerance |
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