In Vitro Reproduction of the Life Cycle of Pythium insidiosum from Kunkers’ Equine and Their Role in the Epidemiology of Pythiosis
Pythium insidiosum is an important pathogen of mammals’ species, including humans. Equine is the main species affected by this oomycete. P. insidiosum requires an aquatic environment to develop its life cycle, and the susceptible hosts are contaminated when they contact the microorganism in swampy a...
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| Veröffentlicht in: | Mycopathologia (1975) 2014-02, Vol.177 (1-2), p.123-127 |
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| creator | da Silva Fonseca, Anelise Oliveira de Avila Botton, Sônia Nogueira, Carlos Eduardo Wayne Corrêa, Bruna Ferraz de Souza Silveira, Júlia de Azevedo, Maria Isabel Maroneze, Beatriz Persici Santurio, Janio Morais Pereira, Daniela Isabel Brayer |
| description | Pythium insidiosum
is an important pathogen of mammals’ species, including humans. Equine is the main species affected by this oomycete.
P. insidiosum
requires an aquatic environment to develop its life cycle, and the susceptible hosts are contaminated when they contact the microorganism in swampy areas. The equine pythiosis is characterized by the formation of irregular masses within the cutaneous lesions, called kunkers, which easily detach from the lesion. From these structures, it is possible to isolate
P. insidiosum
in pure cultures. The present study aimed to reproduce in vitro the life cycle of
P. insidiosum
from kunkers of equine clinical lesions. Fifteen kunkers from different horses were tested. It was observed that the discharge of zoospores occurred after 24–48 h of incubation at 37 °C in, respectively, 40 and 47 % of the kunkers evaluated. Only two samples showed no development of the asexual cycle of
P. insidiosum
under the conditions tested. It was possible to demonstrate that kunkers are able to restart the asexual cycle of
P. insidiosum
. Based on our in vitro results, we highlight the importance of these structures in the epidemiology of the pythiosis, since kunkers can be a potential source of contamination of this oomycete for aquatic environments. |
| doi_str_mv | 10.1007/s11046-013-9720-6 |
| format | Article |
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is an important pathogen of mammals’ species, including humans. Equine is the main species affected by this oomycete.
P. insidiosum
requires an aquatic environment to develop its life cycle, and the susceptible hosts are contaminated when they contact the microorganism in swampy areas. The equine pythiosis is characterized by the formation of irregular masses within the cutaneous lesions, called kunkers, which easily detach from the lesion. From these structures, it is possible to isolate
P. insidiosum
in pure cultures. The present study aimed to reproduce in vitro the life cycle of
P. insidiosum
from kunkers of equine clinical lesions. Fifteen kunkers from different horses were tested. It was observed that the discharge of zoospores occurred after 24–48 h of incubation at 37 °C in, respectively, 40 and 47 % of the kunkers evaluated. Only two samples showed no development of the asexual cycle of
P. insidiosum
under the conditions tested. It was possible to demonstrate that kunkers are able to restart the asexual cycle of
P. insidiosum
. Based on our in vitro results, we highlight the importance of these structures in the epidemiology of the pythiosis, since kunkers can be a potential source of contamination of this oomycete for aquatic environments.</description><identifier>ISSN: 0301-486X</identifier><identifier>EISSN: 1573-0832</identifier><identifier>DOI: 10.1007/s11046-013-9720-6</identifier><identifier>PMID: 24326464</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Animals ; Biomedical and Life Sciences ; Epidemiology ; Eukaryotic Microbiology ; Horse Diseases - epidemiology ; Horse Diseases - microbiology ; Horses - microbiology ; Life Sciences ; Medical Microbiology ; Microbial Ecology ; Microbiology ; Microscopy ; Pathogens ; Plant Sciences ; Pythiosis - epidemiology ; Pythiosis - microbiology ; Pythiosis - veterinary ; Pythium - isolation & purification ; Reproduction, Asexual</subject><ispartof>Mycopathologia (1975), 2014-02, Vol.177 (1-2), p.123-127</ispartof><rights>Springer Science+Business Media Dordrecht 2013</rights><rights>COPYRIGHT 2014 Springer</rights><rights>Springer Science+Business Media Dordrecht 2014</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c473t-cf0e0b779324d82ba2c8a581629365ac3a66425101187ee1a064bf38728df59f3</citedby><cites>FETCH-LOGICAL-c473t-cf0e0b779324d82ba2c8a581629365ac3a66425101187ee1a064bf38728df59f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11046-013-9720-6$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11046-013-9720-6$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>315,781,785,27926,27927,41490,42559,51321</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24326464$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>da Silva Fonseca, Anelise Oliveira</creatorcontrib><creatorcontrib>de Avila Botton, Sônia</creatorcontrib><creatorcontrib>Nogueira, Carlos Eduardo Wayne</creatorcontrib><creatorcontrib>Corrêa, Bruna Ferraz</creatorcontrib><creatorcontrib>de Souza Silveira, Júlia</creatorcontrib><creatorcontrib>de Azevedo, Maria Isabel</creatorcontrib><creatorcontrib>Maroneze, Beatriz Persici</creatorcontrib><creatorcontrib>Santurio, Janio Morais</creatorcontrib><creatorcontrib>Pereira, Daniela Isabel Brayer</creatorcontrib><title>In Vitro Reproduction of the Life Cycle of Pythium insidiosum from Kunkers’ Equine and Their Role in the Epidemiology of Pythiosis</title><title>Mycopathologia (1975)</title><addtitle>Mycopathologia</addtitle><addtitle>Mycopathologia</addtitle><description>Pythium insidiosum
is an important pathogen of mammals’ species, including humans. Equine is the main species affected by this oomycete.
P. insidiosum
requires an aquatic environment to develop its life cycle, and the susceptible hosts are contaminated when they contact the microorganism in swampy areas. The equine pythiosis is characterized by the formation of irregular masses within the cutaneous lesions, called kunkers, which easily detach from the lesion. From these structures, it is possible to isolate
P. insidiosum
in pure cultures. The present study aimed to reproduce in vitro the life cycle of
P. insidiosum
from kunkers of equine clinical lesions. Fifteen kunkers from different horses were tested. It was observed that the discharge of zoospores occurred after 24–48 h of incubation at 37 °C in, respectively, 40 and 47 % of the kunkers evaluated. Only two samples showed no development of the asexual cycle of
P. insidiosum
under the conditions tested. It was possible to demonstrate that kunkers are able to restart the asexual cycle of
P. insidiosum
. Based on our in vitro results, we highlight the importance of these structures in the epidemiology of the pythiosis, since kunkers can be a potential source of contamination of this oomycete for aquatic environments.</description><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Epidemiology</subject><subject>Eukaryotic Microbiology</subject><subject>Horse Diseases - epidemiology</subject><subject>Horse Diseases - microbiology</subject><subject>Horses - microbiology</subject><subject>Life Sciences</subject><subject>Medical Microbiology</subject><subject>Microbial Ecology</subject><subject>Microbiology</subject><subject>Microscopy</subject><subject>Pathogens</subject><subject>Plant Sciences</subject><subject>Pythiosis - epidemiology</subject><subject>Pythiosis - microbiology</subject><subject>Pythiosis - veterinary</subject><subject>Pythium - isolation & purification</subject><subject>Reproduction, Asexual</subject><issn>0301-486X</issn><issn>1573-0832</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1ktFu0zAUhiMEYmXwANwgS9yMiwwf27GTy6kqUK0SqAzEneUmduuR2J2dSPSOC15ir7cnmUPHoAhkyT46_v5fPtafZc8BnwLG4nUEwIznGGheCYJz_iCbQCFojktKHmYTTDHkrORfjrInMV5inFQgHmdHhFHCGWeT7Mfcoc-2Dx4t9Tb4Zqh76x3yBvUbjRbWaDTd1a0eOx92_cYOHbIu2sb6mEoTfIfOB_dVh3jz_RrNrgbrNFKuQRcbbQNa-qS17qfbbGsb3Vnf-vXu3s9HG59mj4xqo352dx5nn97MLqbv8sX7t_Pp2SKvmaB9Xhus8UqIihLWlGSlSF2qogROKsoLVVPFOSMFYIBSaA0Kc7YytBSkbExRGXqcnex906RXg4697Gysddsqp_0QJbCqAgplAQl9-Rd66Yfg0utGinGBK2C_qbVqtbTO-D6oejSVZ1RAyUjaEnX6Dyqt8Tdq77SxqX8geHUgSEyvv_VrNcQo5x-Xhyzs2Tr4GIM2chtsp8JOApZjSuQ-JTKlRI4pkTxpXtwNN6w63dwrfsUiAWQPxHTl1jr8Mf1_XW8BAvbFPQ</recordid><startdate>20140201</startdate><enddate>20140201</enddate><creator>da Silva Fonseca, Anelise Oliveira</creator><creator>de Avila Botton, Sônia</creator><creator>Nogueira, Carlos Eduardo Wayne</creator><creator>Corrêa, Bruna Ferraz</creator><creator>de Souza Silveira, Júlia</creator><creator>de Azevedo, Maria Isabel</creator><creator>Maroneze, Beatriz Persici</creator><creator>Santurio, Janio Morais</creator><creator>Pereira, Daniela Isabel Brayer</creator><general>Springer Netherlands</general><general>Springer</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20140201</creationdate><title>In Vitro Reproduction of the Life Cycle of Pythium insidiosum from Kunkers’ Equine and Their Role in the Epidemiology of Pythiosis</title><author>da Silva Fonseca, Anelise Oliveira ; 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is an important pathogen of mammals’ species, including humans. Equine is the main species affected by this oomycete.
P. insidiosum
requires an aquatic environment to develop its life cycle, and the susceptible hosts are contaminated when they contact the microorganism in swampy areas. The equine pythiosis is characterized by the formation of irregular masses within the cutaneous lesions, called kunkers, which easily detach from the lesion. From these structures, it is possible to isolate
P. insidiosum
in pure cultures. The present study aimed to reproduce in vitro the life cycle of
P. insidiosum
from kunkers of equine clinical lesions. Fifteen kunkers from different horses were tested. It was observed that the discharge of zoospores occurred after 24–48 h of incubation at 37 °C in, respectively, 40 and 47 % of the kunkers evaluated. Only two samples showed no development of the asexual cycle of
P. insidiosum
under the conditions tested. It was possible to demonstrate that kunkers are able to restart the asexual cycle of
P. insidiosum
. Based on our in vitro results, we highlight the importance of these structures in the epidemiology of the pythiosis, since kunkers can be a potential source of contamination of this oomycete for aquatic environments.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>24326464</pmid><doi>10.1007/s11046-013-9720-6</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Mycopathologia (1975), 2014-02, Vol.177 (1-2), p.123-127 |
| issn | 0301-486X 1573-0832 |
| language | eng |
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| source | MEDLINE; SpringerNature Journals |
| subjects | Animals Biomedical and Life Sciences Epidemiology Eukaryotic Microbiology Horse Diseases - epidemiology Horse Diseases - microbiology Horses - microbiology Life Sciences Medical Microbiology Microbial Ecology Microbiology Microscopy Pathogens Plant Sciences Pythiosis - epidemiology Pythiosis - microbiology Pythiosis - veterinary Pythium - isolation & purification Reproduction, Asexual |
| title | In Vitro Reproduction of the Life Cycle of Pythium insidiosum from Kunkers’ Equine and Their Role in the Epidemiology of Pythiosis |
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