Experimental evidence for a 9-binding subsite of Bacillus licheniformis thermostable α-amylase

•B. licheniformis α-amylase produces mainly maltohexaose from maltooctaose and maltononaose.•Maltononaose gave the smallest Km value and the highest kcat.•The enzyme has nine subsites: six in the non-reducing and three at the reducing end-binding site. The action pattern of Bacillus licheniformis th...

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Veröffentlicht in:	FEBS letters 2014-02, Vol.588 (4), p.620-624
Hauptverfasser:	Tran, Phuong Lan, Lee, Jin-Sil, Park, Kwan-Hwa
Format:	Artikel
Sprache:	eng
Schlagworte:	alpha-Amylases - chemistry alpha-Amylases - metabolism Bacillus - enzymology Bacillus licheniformis thermostable α-amylase Binding Sites Enzyme Stability High-performance anion exchange chromatography Kinetic parameter Kinetics Linear maltooligosaccharides Models, Molecular Oligosaccharides - metabolism Protein Conformation Subsite affinity Temperature
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