Cardioprotective effect of vincristine on isoproterenol-induced myocardial necrosis in rats
This study investigated the protective effect of vincristine (VCR) on isoproterenol (ISO)-induced cardiac necrosis (CN) in rats. Animals (n=7 in each group) were pretreated with vincristine (25µg/kg) intraperitoneal (i.p.) daily in 5-day cycles with 2 days pause between cycles using a 5-day-on, 2-da...
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| Veröffentlicht in: | European journal of pharmacology 2014-01, Vol.723, p.451-458 |
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| creator | Panda, Sunanda Kar, Anand Ramamurthy, Vilayanoor |
| description | This study investigated the protective effect of vincristine (VCR) on isoproterenol (ISO)-induced cardiac necrosis (CN) in rats. Animals (n=7 in each group) were pretreated with vincristine (25µg/kg) intraperitoneal (i.p.) daily in 5-day cycles with 2 days pause between cycles using a 5-day-on, 2-day-off schedule for two weeks and then intoxicated with isoproterenol (100mg/kg, s.c., for 2 consecutive days). ISO-induced myocardial damage was indicated by changes in electrocardiographic (ECG) patterns, increased activities of marker enzymes such as creatine kinase-MB, serum glutamate pyruvate transaminase and lactate dehydrogenase and the levels of troponin-T in the serum. The levels of lipid peroxide products, (thiobarbituric acid reactive substances (TBARS) and lipid hydroperoxides (HP)) were increased with a parallel decrease in the activities of antioxidants (superoxide dismutase, catalase, glutathione peroxidase and reduced glutathione) in ISO-induced rats. Furthermore, ISO-induced rats showed increase in the activities of membrane bound enzymes such as Ca2+-ATPase and Mg2+-ATPase with a decreased activity of Na+/K+-ATPase. Triphenyl tetrazolium chloride (TTC) staining of the heart section showed increased area of necrosis in ISO-induced rats. Pretreatment with VCR (25µg/kg) eliminated all ISO-induced biochemical and histopathological changes, and decreased the myocardial necrosis to a greater extent. Transmission electron microscopic findings on the structure of the heart mitochondria confirmed the protective effects of VCR. Present study provides first scientific report on protective effect of vincristine against ISO-induced cardiac damage in rats. |
| doi_str_mv | 10.1016/j.ejphar.2013.10.049 |
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Animals (n=7 in each group) were pretreated with vincristine (25µg/kg) intraperitoneal (i.p.) daily in 5-day cycles with 2 days pause between cycles using a 5-day-on, 2-day-off schedule for two weeks and then intoxicated with isoproterenol (100mg/kg, s.c., for 2 consecutive days). ISO-induced myocardial damage was indicated by changes in electrocardiographic (ECG) patterns, increased activities of marker enzymes such as creatine kinase-MB, serum glutamate pyruvate transaminase and lactate dehydrogenase and the levels of troponin-T in the serum. The levels of lipid peroxide products, (thiobarbituric acid reactive substances (TBARS) and lipid hydroperoxides (HP)) were increased with a parallel decrease in the activities of antioxidants (superoxide dismutase, catalase, glutathione peroxidase and reduced glutathione) in ISO-induced rats. Furthermore, ISO-induced rats showed increase in the activities of membrane bound enzymes such as Ca2+-ATPase and Mg2+-ATPase with a decreased activity of Na+/K+-ATPase. Triphenyl tetrazolium chloride (TTC) staining of the heart section showed increased area of necrosis in ISO-induced rats. Pretreatment with VCR (25µg/kg) eliminated all ISO-induced biochemical and histopathological changes, and decreased the myocardial necrosis to a greater extent. Transmission electron microscopic findings on the structure of the heart mitochondria confirmed the protective effects of VCR. Present study provides first scientific report on protective effect of vincristine against ISO-induced cardiac damage in rats.</description><identifier>ISSN: 0014-2999</identifier><identifier>EISSN: 1879-0712</identifier><identifier>DOI: 10.1016/j.ejphar.2013.10.049</identifier><identifier>PMID: 24201307</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Adenosine Triphosphatases - metabolism ; Animals ; Cardiac necrosis ; Cardiotonic Agents - pharmacology ; Cardiotonic Agents - therapeutic use ; Catalase - metabolism ; Free radicals ; Glutathione - metabolism ; Glutathione Peroxidase - metabolism ; Isoproterenol ; Lipid Peroxidation - drug effects ; Microscopy, Electron, Transmission ; Myocardium - metabolism ; Myocardium - pathology ; Myocardium - ultrastructure ; Necrosis - chemically induced ; Necrosis - drug therapy ; Necrosis - metabolism ; Necrosis - pathology ; Rats ; Rats, Wistar ; Superoxide Dismutase - metabolism ; Transmission electron microscopy ; Vincristine ; Vincristine - pharmacology ; Vincristine - therapeutic use</subject><ispartof>European journal of pharmacology, 2014-01, Vol.723, p.451-458</ispartof><rights>2013 Elsevier B.V.</rights><rights>2013 Published by Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c362t-528b344bf3443dacd181f6d11112eb0ee5a0db3175ee2f04a80c4aa0bd9171243</citedby><cites>FETCH-LOGICAL-c362t-528b344bf3443dacd181f6d11112eb0ee5a0db3175ee2f04a80c4aa0bd9171243</cites><orcidid>0000-0002-1354-2664</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ejphar.2013.10.049$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27928,27929,45999</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24201307$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Panda, Sunanda</creatorcontrib><creatorcontrib>Kar, Anand</creatorcontrib><creatorcontrib>Ramamurthy, Vilayanoor</creatorcontrib><title>Cardioprotective effect of vincristine on isoproterenol-induced myocardial necrosis in rats</title><title>European journal of pharmacology</title><addtitle>Eur J Pharmacol</addtitle><description>This study investigated the protective effect of vincristine (VCR) on isoproterenol (ISO)-induced cardiac necrosis (CN) in rats. Animals (n=7 in each group) were pretreated with vincristine (25µg/kg) intraperitoneal (i.p.) daily in 5-day cycles with 2 days pause between cycles using a 5-day-on, 2-day-off schedule for two weeks and then intoxicated with isoproterenol (100mg/kg, s.c., for 2 consecutive days). ISO-induced myocardial damage was indicated by changes in electrocardiographic (ECG) patterns, increased activities of marker enzymes such as creatine kinase-MB, serum glutamate pyruvate transaminase and lactate dehydrogenase and the levels of troponin-T in the serum. The levels of lipid peroxide products, (thiobarbituric acid reactive substances (TBARS) and lipid hydroperoxides (HP)) were increased with a parallel decrease in the activities of antioxidants (superoxide dismutase, catalase, glutathione peroxidase and reduced glutathione) in ISO-induced rats. Furthermore, ISO-induced rats showed increase in the activities of membrane bound enzymes such as Ca2+-ATPase and Mg2+-ATPase with a decreased activity of Na+/K+-ATPase. Triphenyl tetrazolium chloride (TTC) staining of the heart section showed increased area of necrosis in ISO-induced rats. Pretreatment with VCR (25µg/kg) eliminated all ISO-induced biochemical and histopathological changes, and decreased the myocardial necrosis to a greater extent. Transmission electron microscopic findings on the structure of the heart mitochondria confirmed the protective effects of VCR. Present study provides first scientific report on protective effect of vincristine against ISO-induced cardiac damage in rats.</description><subject>Adenosine Triphosphatases - metabolism</subject><subject>Animals</subject><subject>Cardiac necrosis</subject><subject>Cardiotonic Agents - pharmacology</subject><subject>Cardiotonic Agents - therapeutic use</subject><subject>Catalase - metabolism</subject><subject>Free radicals</subject><subject>Glutathione - metabolism</subject><subject>Glutathione Peroxidase - metabolism</subject><subject>Isoproterenol</subject><subject>Lipid Peroxidation - drug effects</subject><subject>Microscopy, Electron, Transmission</subject><subject>Myocardium - metabolism</subject><subject>Myocardium - pathology</subject><subject>Myocardium - ultrastructure</subject><subject>Necrosis - chemically induced</subject><subject>Necrosis - drug therapy</subject><subject>Necrosis - metabolism</subject><subject>Necrosis - pathology</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Superoxide Dismutase - metabolism</subject><subject>Transmission electron microscopy</subject><subject>Vincristine</subject><subject>Vincristine - pharmacology</subject><subject>Vincristine - therapeutic use</subject><issn>0014-2999</issn><issn>1879-0712</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LAzEQhoMotn78A5EcvWydZLPd5iJI8QsKXvTkIWSTWUzZJjXZFvrvzbLq0TlkwvC-8_EQcsVgxoDNb9czXG8_dZxxYGUuzUDIIzJli1oWUDN-TKYATBRcSjkhZymtAaCSvDolEy4GE9RT8rHU0bqwjaFH07s9Umzb_KOhpXvnTXSpdx5p8NSlURbRh65w3u4MWro5BDO00B31aGJILlHnadR9uiAnre4SXv7kc_L--PC2fC5Wr08vy_tVYco574uKL5pSiKbNT2m1sWzB2rllOTg2gFhpsE3J6gqRtyD0AozQGhorWb5TlOfkZuyb1_vaYerVxiWDXac9hl1STEjJeJ2pZakYpcOmKWKrttFtdDwoBmrAqtZqxKoGQkM1Y822658Ju2aD9s_0yzEL7kYB5jv3DqNKxqHPgFzMNJUN7v8J356ljFI</recordid><startdate>20140115</startdate><enddate>20140115</enddate><creator>Panda, Sunanda</creator><creator>Kar, Anand</creator><creator>Ramamurthy, Vilayanoor</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-1354-2664</orcidid></search><sort><creationdate>20140115</creationdate><title>Cardioprotective effect of vincristine on isoproterenol-induced myocardial necrosis in rats</title><author>Panda, Sunanda ; Kar, Anand ; Ramamurthy, Vilayanoor</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c362t-528b344bf3443dacd181f6d11112eb0ee5a0db3175ee2f04a80c4aa0bd9171243</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Adenosine Triphosphatases - metabolism</topic><topic>Animals</topic><topic>Cardiac necrosis</topic><topic>Cardiotonic Agents - pharmacology</topic><topic>Cardiotonic Agents - therapeutic use</topic><topic>Catalase - metabolism</topic><topic>Free radicals</topic><topic>Glutathione - metabolism</topic><topic>Glutathione Peroxidase - metabolism</topic><topic>Isoproterenol</topic><topic>Lipid Peroxidation - drug effects</topic><topic>Microscopy, Electron, Transmission</topic><topic>Myocardium - metabolism</topic><topic>Myocardium - pathology</topic><topic>Myocardium - ultrastructure</topic><topic>Necrosis - chemically induced</topic><topic>Necrosis - drug therapy</topic><topic>Necrosis - metabolism</topic><topic>Necrosis - pathology</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Superoxide Dismutase - metabolism</topic><topic>Transmission electron microscopy</topic><topic>Vincristine</topic><topic>Vincristine - pharmacology</topic><topic>Vincristine - therapeutic use</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Panda, Sunanda</creatorcontrib><creatorcontrib>Kar, Anand</creatorcontrib><creatorcontrib>Ramamurthy, Vilayanoor</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Panda, Sunanda</au><au>Kar, Anand</au><au>Ramamurthy, Vilayanoor</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cardioprotective effect of vincristine on isoproterenol-induced myocardial necrosis in rats</atitle><jtitle>European journal of pharmacology</jtitle><addtitle>Eur J Pharmacol</addtitle><date>2014-01-15</date><risdate>2014</risdate><volume>723</volume><spage>451</spage><epage>458</epage><pages>451-458</pages><issn>0014-2999</issn><eissn>1879-0712</eissn><abstract>This study investigated the protective effect of vincristine (VCR) on isoproterenol (ISO)-induced cardiac necrosis (CN) in rats. Animals (n=7 in each group) were pretreated with vincristine (25µg/kg) intraperitoneal (i.p.) daily in 5-day cycles with 2 days pause between cycles using a 5-day-on, 2-day-off schedule for two weeks and then intoxicated with isoproterenol (100mg/kg, s.c., for 2 consecutive days). ISO-induced myocardial damage was indicated by changes in electrocardiographic (ECG) patterns, increased activities of marker enzymes such as creatine kinase-MB, serum glutamate pyruvate transaminase and lactate dehydrogenase and the levels of troponin-T in the serum. The levels of lipid peroxide products, (thiobarbituric acid reactive substances (TBARS) and lipid hydroperoxides (HP)) were increased with a parallel decrease in the activities of antioxidants (superoxide dismutase, catalase, glutathione peroxidase and reduced glutathione) in ISO-induced rats. Furthermore, ISO-induced rats showed increase in the activities of membrane bound enzymes such as Ca2+-ATPase and Mg2+-ATPase with a decreased activity of Na+/K+-ATPase. Triphenyl tetrazolium chloride (TTC) staining of the heart section showed increased area of necrosis in ISO-induced rats. Pretreatment with VCR (25µg/kg) eliminated all ISO-induced biochemical and histopathological changes, and decreased the myocardial necrosis to a greater extent. Transmission electron microscopic findings on the structure of the heart mitochondria confirmed the protective effects of VCR. Present study provides first scientific report on protective effect of vincristine against ISO-induced cardiac damage in rats.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>24201307</pmid><doi>10.1016/j.ejphar.2013.10.049</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-1354-2664</orcidid></addata></record> |
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| subjects | Adenosine Triphosphatases - metabolism Animals Cardiac necrosis Cardiotonic Agents - pharmacology Cardiotonic Agents - therapeutic use Catalase - metabolism Free radicals Glutathione - metabolism Glutathione Peroxidase - metabolism Isoproterenol Lipid Peroxidation - drug effects Microscopy, Electron, Transmission Myocardium - metabolism Myocardium - pathology Myocardium - ultrastructure Necrosis - chemically induced Necrosis - drug therapy Necrosis - metabolism Necrosis - pathology Rats Rats, Wistar Superoxide Dismutase - metabolism Transmission electron microscopy Vincristine Vincristine - pharmacology Vincristine - therapeutic use |
| title | Cardioprotective effect of vincristine on isoproterenol-induced myocardial necrosis in rats |
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