Matrix-assisted laser desorption/ionization imaging mass spectrometry revealed traces of dental problem associated with dental structure
Periodontal disease is a serious dental problem because it does not heal naturally and leads to tooth loss. In periodontal disease, inflammation at periodontal tissue is thought as predominant, and its effect against tooth itself remains unclear. In this study, we applied matrix-assisted laser desor...
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| Veröffentlicht in: | Analytical and bioanalytical chemistry 2014-02, Vol.406 (5), p.1355-1363 |
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| creator | Hirano, Hirokazu Masaki, Noritaka Hayasaka, Takahiro Watanabe, Yoshiko Masumoto, Kazuma Nagata, Tetsuji Katou, Fuminori Setou, Mitsutoshi |
| description | Periodontal disease is a serious dental problem because it does not heal naturally and leads to tooth loss. In periodontal disease, inflammation at periodontal tissue is thought as predominant, and its effect against tooth itself remains unclear. In this study, we applied matrix-assisted laser desorption/ionization imaging mass spectrometry (IMS) to teeth for the first time. By comparing anatomical structure of tooth affected with periodontal disease with normal ones, we analyzed traces of the disease on tooth. We found signals characteristic of enamel, dentin, and dental pulp, respectively, in mass spectra obtained from normal teeth. Ion images reconstructed using these signals showed anatomical structures of the tooth clearly. Next, we performed IMS upon teeth of periodontal disease. Overall characteristic of the mass spectrum appeared similar to normal ones. However, ion images reconstructed using signals from the tooth of periodontal disease revealed loss of periodontal ligament visualized together with dental pulp in normal teeth. Moreover, ion image clearly depicted an accumulation of signal at
m
/
z
496.3 at root surface. Such an accumulation that cannot be examined only from mass spectrum was revealed by utilization of IMS. Recent studies about inflammation revealed that the signal at
m
/
z
496.3 reflects lyso-phosphatidylcholine (LPC). Infiltration of the signal is statistically significant, and its intensity profile exhibited the influence has reached deeply into the tooth. This suggests that influence of periodontal disease is not only inflammation of periodontal tissue but also infiltration of LPC to root surface, and therefore, anti-inflammatory treatment is required besides conventional treatments. |
| doi_str_mv | 10.1007/s00216-013-7075-y |
| format | Article |
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m
/
z
496.3 at root surface. Such an accumulation that cannot be examined only from mass spectrum was revealed by utilization of IMS. Recent studies about inflammation revealed that the signal at
m
/
z
496.3 reflects lyso-phosphatidylcholine (LPC). Infiltration of the signal is statistically significant, and its intensity profile exhibited the influence has reached deeply into the tooth. This suggests that influence of periodontal disease is not only inflammation of periodontal tissue but also infiltration of LPC to root surface, and therefore, anti-inflammatory treatment is required besides conventional treatments.</description><identifier>ISSN: 1618-2642</identifier><identifier>EISSN: 1618-2650</identifier><identifier>DOI: 10.1007/s00216-013-7075-y</identifier><identifier>PMID: 23727733</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Analytical Chemistry ; Bacteria ; Biochemistry ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Choline ; Dental enamel ; Dental Enamel - metabolism ; Dental Enamel - pathology ; Dental pulp ; Dental Pulp - metabolism ; Dental Pulp - pathology ; Dentin ; Dentin - metabolism ; Dentin - pathology ; Desorption ; Diagnosis ; Diagnostic Imaging - methods ; Disease prevention ; Food Science ; Gum disease ; Health aspects ; Humans ; Image Processing, Computer-Assisted ; Infiltration ; Inflammation ; Inflammation - diagnosis ; Inflammation - metabolism ; Inflammation - pathology ; Ionization ; Laboratory Medicine ; Lasers ; Ligaments ; Lysophosphatidylcholines - analysis ; Lysophosphatidylcholines - metabolism ; Mass spectra ; Mass spectrometry ; Methods ; Monitoring/Environmental Analysis ; Morphology ; Periodontal disease ; Periodontal diseases ; Periodontal Diseases - diagnosis ; Periodontal Diseases - metabolism ; Periodontal Diseases - pathology ; Periodontal Ligament - metabolism ; Periodontal Ligament - pathology ; Research Paper ; Scientific imaging ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods ; Teeth ; Tooth Root - metabolism ; Tooth Root - pathology</subject><ispartof>Analytical and bioanalytical chemistry, 2014-02, Vol.406 (5), p.1355-1363</ispartof><rights>Springer-Verlag Berlin Heidelberg 2013</rights><rights>COPYRIGHT 2014 Springer</rights><rights>Springer-Verlag Berlin Heidelberg 2014</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c559t-b087b2248627d18e85589953addd388395f3365f1c89fafa8e8cfdf5e52e8fc53</citedby><cites>FETCH-LOGICAL-c559t-b087b2248627d18e85589953addd388395f3365f1c89fafa8e8cfdf5e52e8fc53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00216-013-7075-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00216-013-7075-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51297</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23727733$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hirano, Hirokazu</creatorcontrib><creatorcontrib>Masaki, Noritaka</creatorcontrib><creatorcontrib>Hayasaka, Takahiro</creatorcontrib><creatorcontrib>Watanabe, Yoshiko</creatorcontrib><creatorcontrib>Masumoto, Kazuma</creatorcontrib><creatorcontrib>Nagata, Tetsuji</creatorcontrib><creatorcontrib>Katou, Fuminori</creatorcontrib><creatorcontrib>Setou, Mitsutoshi</creatorcontrib><title>Matrix-assisted laser desorption/ionization imaging mass spectrometry revealed traces of dental problem associated with dental structure</title><title>Analytical and bioanalytical chemistry</title><addtitle>Anal Bioanal Chem</addtitle><addtitle>Anal Bioanal Chem</addtitle><description>Periodontal disease is a serious dental problem because it does not heal naturally and leads to tooth loss. In periodontal disease, inflammation at periodontal tissue is thought as predominant, and its effect against tooth itself remains unclear. In this study, we applied matrix-assisted laser desorption/ionization imaging mass spectrometry (IMS) to teeth for the first time. By comparing anatomical structure of tooth affected with periodontal disease with normal ones, we analyzed traces of the disease on tooth. We found signals characteristic of enamel, dentin, and dental pulp, respectively, in mass spectra obtained from normal teeth. Ion images reconstructed using these signals showed anatomical structures of the tooth clearly. Next, we performed IMS upon teeth of periodontal disease. Overall characteristic of the mass spectrum appeared similar to normal ones. However, ion images reconstructed using signals from the tooth of periodontal disease revealed loss of periodontal ligament visualized together with dental pulp in normal teeth. Moreover, ion image clearly depicted an accumulation of signal at
m
/
z
496.3 at root surface. Such an accumulation that cannot be examined only from mass spectrum was revealed by utilization of IMS. Recent studies about inflammation revealed that the signal at
m
/
z
496.3 reflects lyso-phosphatidylcholine (LPC). Infiltration of the signal is statistically significant, and its intensity profile exhibited the influence has reached deeply into the tooth. This suggests that influence of periodontal disease is not only inflammation of periodontal tissue but also infiltration of LPC to root surface, and therefore, anti-inflammatory treatment is required besides conventional treatments.</description><subject>Analytical Chemistry</subject><subject>Bacteria</subject><subject>Biochemistry</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Choline</subject><subject>Dental enamel</subject><subject>Dental Enamel - metabolism</subject><subject>Dental Enamel - pathology</subject><subject>Dental pulp</subject><subject>Dental Pulp - metabolism</subject><subject>Dental Pulp - pathology</subject><subject>Dentin</subject><subject>Dentin - metabolism</subject><subject>Dentin - pathology</subject><subject>Desorption</subject><subject>Diagnosis</subject><subject>Diagnostic Imaging - methods</subject><subject>Disease prevention</subject><subject>Food Science</subject><subject>Gum disease</subject><subject>Health aspects</subject><subject>Humans</subject><subject>Image Processing, Computer-Assisted</subject><subject>Infiltration</subject><subject>Inflammation</subject><subject>Inflammation - diagnosis</subject><subject>Inflammation - metabolism</subject><subject>Inflammation - pathology</subject><subject>Ionization</subject><subject>Laboratory Medicine</subject><subject>Lasers</subject><subject>Ligaments</subject><subject>Lysophosphatidylcholines - analysis</subject><subject>Lysophosphatidylcholines - metabolism</subject><subject>Mass spectra</subject><subject>Mass spectrometry</subject><subject>Methods</subject><subject>Monitoring/Environmental Analysis</subject><subject>Morphology</subject><subject>Periodontal disease</subject><subject>Periodontal diseases</subject><subject>Periodontal Diseases - diagnosis</subject><subject>Periodontal Diseases - metabolism</subject><subject>Periodontal Diseases - pathology</subject><subject>Periodontal Ligament - metabolism</subject><subject>Periodontal Ligament - pathology</subject><subject>Research Paper</subject><subject>Scientific imaging</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods</subject><subject>Teeth</subject><subject>Tooth Root - 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metabolism</topic><topic>Dental Enamel - pathology</topic><topic>Dental pulp</topic><topic>Dental Pulp - metabolism</topic><topic>Dental Pulp - pathology</topic><topic>Dentin</topic><topic>Dentin - metabolism</topic><topic>Dentin - pathology</topic><topic>Desorption</topic><topic>Diagnosis</topic><topic>Diagnostic Imaging - methods</topic><topic>Disease prevention</topic><topic>Food Science</topic><topic>Gum disease</topic><topic>Health aspects</topic><topic>Humans</topic><topic>Image Processing, Computer-Assisted</topic><topic>Infiltration</topic><topic>Inflammation</topic><topic>Inflammation - diagnosis</topic><topic>Inflammation - metabolism</topic><topic>Inflammation - pathology</topic><topic>Ionization</topic><topic>Laboratory Medicine</topic><topic>Lasers</topic><topic>Ligaments</topic><topic>Lysophosphatidylcholines - analysis</topic><topic>Lysophosphatidylcholines - metabolism</topic><topic>Mass spectra</topic><topic>Mass spectrometry</topic><topic>Methods</topic><topic>Monitoring/Environmental Analysis</topic><topic>Morphology</topic><topic>Periodontal disease</topic><topic>Periodontal diseases</topic><topic>Periodontal Diseases - diagnosis</topic><topic>Periodontal Diseases - metabolism</topic><topic>Periodontal Diseases - pathology</topic><topic>Periodontal Ligament - metabolism</topic><topic>Periodontal Ligament - pathology</topic><topic>Research Paper</topic><topic>Scientific imaging</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods</topic><topic>Teeth</topic><topic>Tooth Root - metabolism</topic><topic>Tooth Root - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hirano, Hirokazu</creatorcontrib><creatorcontrib>Masaki, Noritaka</creatorcontrib><creatorcontrib>Hayasaka, Takahiro</creatorcontrib><creatorcontrib>Watanabe, Yoshiko</creatorcontrib><creatorcontrib>Masumoto, Kazuma</creatorcontrib><creatorcontrib>Nagata, Tetsuji</creatorcontrib><creatorcontrib>Katou, Fuminori</creatorcontrib><creatorcontrib>Setou, Mitsutoshi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>ProQuest Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Database (Proquest)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Databases</collection><collection>Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>SciTech Premium Collection</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Materials Science Database</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Biological Sciences</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Materials science collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical and bioanalytical chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hirano, Hirokazu</au><au>Masaki, Noritaka</au><au>Hayasaka, Takahiro</au><au>Watanabe, Yoshiko</au><au>Masumoto, Kazuma</au><au>Nagata, Tetsuji</au><au>Katou, Fuminori</au><au>Setou, Mitsutoshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Matrix-assisted laser desorption/ionization imaging mass spectrometry revealed traces of dental problem associated with dental structure</atitle><jtitle>Analytical and bioanalytical chemistry</jtitle><stitle>Anal Bioanal Chem</stitle><addtitle>Anal Bioanal Chem</addtitle><date>2014-02-01</date><risdate>2014</risdate><volume>406</volume><issue>5</issue><spage>1355</spage><epage>1363</epage><pages>1355-1363</pages><issn>1618-2642</issn><eissn>1618-2650</eissn><abstract>Periodontal disease is a serious dental problem because it does not heal naturally and leads to tooth loss. In periodontal disease, inflammation at periodontal tissue is thought as predominant, and its effect against tooth itself remains unclear. In this study, we applied matrix-assisted laser desorption/ionization imaging mass spectrometry (IMS) to teeth for the first time. By comparing anatomical structure of tooth affected with periodontal disease with normal ones, we analyzed traces of the disease on tooth. We found signals characteristic of enamel, dentin, and dental pulp, respectively, in mass spectra obtained from normal teeth. Ion images reconstructed using these signals showed anatomical structures of the tooth clearly. Next, we performed IMS upon teeth of periodontal disease. Overall characteristic of the mass spectrum appeared similar to normal ones. However, ion images reconstructed using signals from the tooth of periodontal disease revealed loss of periodontal ligament visualized together with dental pulp in normal teeth. Moreover, ion image clearly depicted an accumulation of signal at
m
/
z
496.3 at root surface. Such an accumulation that cannot be examined only from mass spectrum was revealed by utilization of IMS. Recent studies about inflammation revealed that the signal at
m
/
z
496.3 reflects lyso-phosphatidylcholine (LPC). Infiltration of the signal is statistically significant, and its intensity profile exhibited the influence has reached deeply into the tooth. This suggests that influence of periodontal disease is not only inflammation of periodontal tissue but also infiltration of LPC to root surface, and therefore, anti-inflammatory treatment is required besides conventional treatments.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>23727733</pmid><doi>10.1007/s00216-013-7075-y</doi><tpages>9</tpages></addata></record> |
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| subjects | Analytical Chemistry Bacteria Biochemistry Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Choline Dental enamel Dental Enamel - metabolism Dental Enamel - pathology Dental pulp Dental Pulp - metabolism Dental Pulp - pathology Dentin Dentin - metabolism Dentin - pathology Desorption Diagnosis Diagnostic Imaging - methods Disease prevention Food Science Gum disease Health aspects Humans Image Processing, Computer-Assisted Infiltration Inflammation Inflammation - diagnosis Inflammation - metabolism Inflammation - pathology Ionization Laboratory Medicine Lasers Ligaments Lysophosphatidylcholines - analysis Lysophosphatidylcholines - metabolism Mass spectra Mass spectrometry Methods Monitoring/Environmental Analysis Morphology Periodontal disease Periodontal diseases Periodontal Diseases - diagnosis Periodontal Diseases - metabolism Periodontal Diseases - pathology Periodontal Ligament - metabolism Periodontal Ligament - pathology Research Paper Scientific imaging Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods Teeth Tooth Root - metabolism Tooth Root - pathology |
| title | Matrix-assisted laser desorption/ionization imaging mass spectrometry revealed traces of dental problem associated with dental structure |
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