Expression of Lactobacillus reuteri Pg4 Collagen-Binding Protein Gene in Lactobacillus casei ATCC 393 Increases Its Adhesion Ability to Caco-2 Cells
The collagen-binding protein gene cnb was cloned from the probiotic Lactobacillus reuteri strain Pg4. The DNA sequence of the cnb gene (792 bp) has an open reading frame encoding 263 amino acids with a calculated molecular weight of 28.5 kDa. The cnb gene was constructed so as to constitutively expr...
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| Veröffentlicht in: | Journal of agricultural and food chemistry 2010-12, Vol.58 (23), p.12182-12191 |
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| container_issue | 23 |
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| container_title | Journal of agricultural and food chemistry |
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| creator | Hsueh, Hsiang-Yun Yueh, Pei-Ying Yu, Bi Zhao, Xin Liu, Je-Ruei |
| description | The collagen-binding protein gene cnb was cloned from the probiotic Lactobacillus reuteri strain Pg4. The DNA sequence of the cnb gene (792 bp) has an open reading frame encoding 263 amino acids with a calculated molecular weight of 28.5 kDa. The cnb gene was constructed so as to constitutively express under the control of the Lactococcus lactis lacA promoter and was transformed into Lactobacillus casei ATCC 393, a strain isolated from dairy products with poor ability to adhere to intestinal epithelial cells. Confocal immunofluorescence microscopic and flow cytometric analysis of the transformed strain Lb. casei pNZ-cnb indicated that Cnb was displayed on its cell surface. Lb. casei pNZ-cnb not only showed a higher ability to adhere to Caco-2 cells but also exhibited a higher competition ability against Escherichia coli O157:H7 and Listeria monocytogenes adhesion to Caco-2 cells than Lb. casei ATCC 393. |
| doi_str_mv | 10.1021/jf1035756 |
| format | Article |
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The DNA sequence of the cnb gene (792 bp) has an open reading frame encoding 263 amino acids with a calculated molecular weight of 28.5 kDa. The cnb gene was constructed so as to constitutively express under the control of the Lactococcus lactis lacA promoter and was transformed into Lactobacillus casei ATCC 393, a strain isolated from dairy products with poor ability to adhere to intestinal epithelial cells. Confocal immunofluorescence microscopic and flow cytometric analysis of the transformed strain Lb. casei pNZ-cnb indicated that Cnb was displayed on its cell surface. Lb. casei pNZ-cnb not only showed a higher ability to adhere to Caco-2 cells but also exhibited a higher competition ability against Escherichia coli O157:H7 and Listeria monocytogenes adhesion to Caco-2 cells than Lb. casei ATCC 393.</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/jf1035756</identifier><identifier>PMID: 21070005</identifier><identifier>CODEN: JAFCAU</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Bacterial Adhesion ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; binding proteins ; Biological and medical sciences ; Caco-2 Cells ; cell culture ; Chemical Aspects of Biotechnology/Molecular Biology ; collagen ; colorectal neoplasms ; dimerization ; Epithelial Cells - microbiology ; Food industries ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; genes ; human cell lines ; Humans ; Intestines - microbiology ; Lactobacillus - genetics ; Lactobacillus - metabolism ; Lactobacillus casei ; Lactobacillus casei - genetics ; Lactobacillus casei - physiology ; Lactobacillus reuteri ; Lactobacillus reuteri Pg4 collagen-binding protein gene ; Open Reading Frames ; probiotics ; Up-Regulation</subject><ispartof>Journal of agricultural and food chemistry, 2010-12, Vol.58 (23), p.12182-12191</ispartof><rights>Copyright © 2010 American Chemical Society</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a435t-99c3d75816257ca7cb96284f04c9d120848e08365983fa0811217a9d5b5ff3343</citedby><cites>FETCH-LOGICAL-a435t-99c3d75816257ca7cb96284f04c9d120848e08365983fa0811217a9d5b5ff3343</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/jf1035756$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/jf1035756$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23598025$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21070005$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hsueh, Hsiang-Yun</creatorcontrib><creatorcontrib>Yueh, Pei-Ying</creatorcontrib><creatorcontrib>Yu, Bi</creatorcontrib><creatorcontrib>Zhao, Xin</creatorcontrib><creatorcontrib>Liu, Je-Ruei</creatorcontrib><title>Expression of Lactobacillus reuteri Pg4 Collagen-Binding Protein Gene in Lactobacillus casei ATCC 393 Increases Its Adhesion Ability to Caco-2 Cells</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>The collagen-binding protein gene cnb was cloned from the probiotic Lactobacillus reuteri strain Pg4. The DNA sequence of the cnb gene (792 bp) has an open reading frame encoding 263 amino acids with a calculated molecular weight of 28.5 kDa. The cnb gene was constructed so as to constitutively express under the control of the Lactococcus lactis lacA promoter and was transformed into Lactobacillus casei ATCC 393, a strain isolated from dairy products with poor ability to adhere to intestinal epithelial cells. Confocal immunofluorescence microscopic and flow cytometric analysis of the transformed strain Lb. casei pNZ-cnb indicated that Cnb was displayed on its cell surface. Lb. casei pNZ-cnb not only showed a higher ability to adhere to Caco-2 cells but also exhibited a higher competition ability against Escherichia coli O157:H7 and Listeria monocytogenes adhesion to Caco-2 cells than Lb. casei ATCC 393.</description><subject>Bacterial Adhesion</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>binding proteins</subject><subject>Biological and medical sciences</subject><subject>Caco-2 Cells</subject><subject>cell culture</subject><subject>Chemical Aspects of Biotechnology/Molecular Biology</subject><subject>collagen</subject><subject>colorectal neoplasms</subject><subject>dimerization</subject><subject>Epithelial Cells - microbiology</subject><subject>Food industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>genes</subject><subject>human cell lines</subject><subject>Humans</subject><subject>Intestines - microbiology</subject><subject>Lactobacillus - genetics</subject><subject>Lactobacillus - metabolism</subject><subject>Lactobacillus casei</subject><subject>Lactobacillus casei - genetics</subject><subject>Lactobacillus casei - physiology</subject><subject>Lactobacillus reuteri</subject><subject>Lactobacillus reuteri Pg4 collagen-binding protein gene</subject><subject>Open Reading Frames</subject><subject>probiotics</subject><subject>Up-Regulation</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkcFuEzEQhi0EoqFw4AXAFyQ4LMzY6137GFalRIpEJdrzyvHawdHGDvau1L4HD4xLQiskTiN7Pv_z-x9CXiN8RGD4aecQuGhF84QsUDCoBKJ8ShZQmpUUDZ6RFznvAECKFp6TM4bQlpNYkF8Xt4dkc_Yx0OjoWpspbrTx4zhnmuw82eTp1bamXRxHvbWh-uzD4MOWXqU4WR_opQ2WlvrvU6Oz9XR53XWUK05XwSRbrjJdTZkuhx_2z8Tlxo9-uqNTpJ02sWK0s-OYX5JnTo_ZvjrVc3Lz5eK6-1qtv12uuuW60jUXU6WU4UMrJDZMtEa3ZqMaJmsHtVEDMpC1tCB5I5TkToNEZNhqNYiNcI7zmp-T90fdQ4o_Z5unfu-zKQ50sHHOPdZKIRMSVEE_HFGTYs7Juv6Q_F6nux6hv19C_7CEwr45yc6bvR0eyL-pF-DdCdDZ6NElHYzPjxwvjoHdc2-PnNOx19tUmJvvDJADqvJlVT8qaZP7XZxTKHn9x9JvfTahOg</recordid><startdate>20101208</startdate><enddate>20101208</enddate><creator>Hsueh, Hsiang-Yun</creator><creator>Yueh, Pei-Ying</creator><creator>Yu, Bi</creator><creator>Zhao, Xin</creator><creator>Liu, Je-Ruei</creator><general>American Chemical Society</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20101208</creationdate><title>Expression of Lactobacillus reuteri Pg4 Collagen-Binding Protein Gene in Lactobacillus casei ATCC 393 Increases Its Adhesion Ability to Caco-2 Cells</title><author>Hsueh, Hsiang-Yun ; Yueh, Pei-Ying ; Yu, Bi ; Zhao, Xin ; Liu, Je-Ruei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a435t-99c3d75816257ca7cb96284f04c9d120848e08365983fa0811217a9d5b5ff3343</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Bacterial Adhesion</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>binding proteins</topic><topic>Biological and medical sciences</topic><topic>Caco-2 Cells</topic><topic>cell culture</topic><topic>Chemical Aspects of Biotechnology/Molecular Biology</topic><topic>collagen</topic><topic>colorectal neoplasms</topic><topic>dimerization</topic><topic>Epithelial Cells - microbiology</topic><topic>Food industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>genes</topic><topic>human cell lines</topic><topic>Humans</topic><topic>Intestines - microbiology</topic><topic>Lactobacillus - genetics</topic><topic>Lactobacillus - metabolism</topic><topic>Lactobacillus casei</topic><topic>Lactobacillus casei - genetics</topic><topic>Lactobacillus casei - physiology</topic><topic>Lactobacillus reuteri</topic><topic>Lactobacillus reuteri Pg4 collagen-binding protein gene</topic><topic>Open Reading Frames</topic><topic>probiotics</topic><topic>Up-Regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hsueh, Hsiang-Yun</creatorcontrib><creatorcontrib>Yueh, Pei-Ying</creatorcontrib><creatorcontrib>Yu, Bi</creatorcontrib><creatorcontrib>Zhao, Xin</creatorcontrib><creatorcontrib>Liu, Je-Ruei</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hsueh, Hsiang-Yun</au><au>Yueh, Pei-Ying</au><au>Yu, Bi</au><au>Zhao, Xin</au><au>Liu, Je-Ruei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of Lactobacillus reuteri Pg4 Collagen-Binding Protein Gene in Lactobacillus casei ATCC 393 Increases Its Adhesion Ability to Caco-2 Cells</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2010-12-08</date><risdate>2010</risdate><volume>58</volume><issue>23</issue><spage>12182</spage><epage>12191</epage><pages>12182-12191</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><coden>JAFCAU</coden><abstract>The collagen-binding protein gene cnb was cloned from the probiotic Lactobacillus reuteri strain Pg4. The DNA sequence of the cnb gene (792 bp) has an open reading frame encoding 263 amino acids with a calculated molecular weight of 28.5 kDa. The cnb gene was constructed so as to constitutively express under the control of the Lactococcus lactis lacA promoter and was transformed into Lactobacillus casei ATCC 393, a strain isolated from dairy products with poor ability to adhere to intestinal epithelial cells. Confocal immunofluorescence microscopic and flow cytometric analysis of the transformed strain Lb. casei pNZ-cnb indicated that Cnb was displayed on its cell surface. Lb. casei pNZ-cnb not only showed a higher ability to adhere to Caco-2 cells but also exhibited a higher competition ability against Escherichia coli O157:H7 and Listeria monocytogenes adhesion to Caco-2 cells than Lb. casei ATCC 393.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>21070005</pmid><doi>10.1021/jf1035756</doi><tpages>10</tpages></addata></record> |
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| source | ACS Publications; MEDLINE |
| subjects | Bacterial Adhesion Bacterial Proteins - genetics Bacterial Proteins - metabolism binding proteins Biological and medical sciences Caco-2 Cells cell culture Chemical Aspects of Biotechnology/Molecular Biology collagen colorectal neoplasms dimerization Epithelial Cells - microbiology Food industries Fundamental and applied biological sciences. Psychology Gene Expression genes human cell lines Humans Intestines - microbiology Lactobacillus - genetics Lactobacillus - metabolism Lactobacillus casei Lactobacillus casei - genetics Lactobacillus casei - physiology Lactobacillus reuteri Lactobacillus reuteri Pg4 collagen-binding protein gene Open Reading Frames probiotics Up-Regulation |
| title | Expression of Lactobacillus reuteri Pg4 Collagen-Binding Protein Gene in Lactobacillus casei ATCC 393 Increases Its Adhesion Ability to Caco-2 Cells |
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