Optimization and Validation of a GC-FID Method for the Determination of Acetone-Butanol-Ethanol Fermentation Products
An improved, simple gas chromatography-flame ionization detection (GC-FID) method was developed for measuring the products of acetone-butanol-ethanol (ABE) fermentation and the combined fermentation/separation processes. The analysis time per sample was reduced to less than 10 min compared to those...
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| Veröffentlicht in: | Journal of chromatographic science 2014-03, Vol.52 (3), p.264-270 |
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| container_title | Journal of chromatographic science |
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| creator | Lin, Xiaoqing Fan, Jiansheng Wen, Qingshi Li, Renjie Jin, Xiaohong Wu, Jinglan Qian, Wenbin Liu, Dong Xie, Jingjing Bai, Jianxin Ying, Hanjie |
| description | An improved, simple gas chromatography-flame ionization detection (GC-FID) method was developed for measuring the products of acetone-butanol-ethanol (ABE) fermentation and the combined fermentation/separation processes. The analysis time per sample was reduced to less than 10 min compared to those of a conventional GC-FID (more than 20 min). The behavior of the compounds in temperature-programmed gas chromatographic runs was predicted using thermodynamic parameters derived from isothermal runs. The optimum temperature programming condition was achieved when the resolution for each peak met the analytical requirement and the analysis time was shortest. With the exception of acetic acid, the detection limits of the presented method for various products were below 10 mg/L. The repeatability and intermediate precision of the method were less than 10% (relative standard deviation). Validation and quantification results demonstrated that this method is a sensitive, reliable and fast alternative for conventional investigation of the adsorption-coupled ABE fermentation process. |
| doi_str_mv | 10.1093/chromsci/bmt022 |
| format | Article |
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The analysis time per sample was reduced to less than 10 min compared to those of a conventional GC-FID (more than 20 min). The behavior of the compounds in temperature-programmed gas chromatographic runs was predicted using thermodynamic parameters derived from isothermal runs. The optimum temperature programming condition was achieved when the resolution for each peak met the analytical requirement and the analysis time was shortest. With the exception of acetic acid, the detection limits of the presented method for various products were below 10 mg/L. The repeatability and intermediate precision of the method were less than 10% (relative standard deviation). Validation and quantification results demonstrated that this method is a sensitive, reliable and fast alternative for conventional investigation of the adsorption-coupled ABE fermentation process.</description><identifier>ISSN: 0021-9665</identifier><identifier>EISSN: 1945-239X</identifier><identifier>DOI: 10.1093/chromsci/bmt022</identifier><identifier>PMID: 23529047</identifier><language>eng</language><publisher>United States: Oxford University Press</publisher><subject>Acetone - analysis ; Acetone - chemistry ; Acetone - isolation & purification ; Acetone - metabolism ; Butanols - analysis ; Butanols - chemistry ; Butanols - isolation & purification ; Butanols - metabolism ; Chromatography, Gas - methods ; Clostridium acetobutylicum - metabolism ; Ethanol - analysis ; Ethanol - chemistry ; Ethanol - isolation & purification ; Ethanol - metabolism ; Fermentation ; Limit of Detection ; Reproducibility of Results</subject><ispartof>Journal of chromatographic science, 2014-03, Vol.52 (3), p.264-270</ispartof><rights>The Author [2013]. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com 2013</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c303t-496a1c5d34bc42304dd84f2bd5f66478c37a7ed3f1fbc926c09cdd72a57f12d73</citedby><cites>FETCH-LOGICAL-c303t-496a1c5d34bc42304dd84f2bd5f66478c37a7ed3f1fbc926c09cdd72a57f12d73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,1579,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23529047$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lin, Xiaoqing</creatorcontrib><creatorcontrib>Fan, Jiansheng</creatorcontrib><creatorcontrib>Wen, Qingshi</creatorcontrib><creatorcontrib>Li, Renjie</creatorcontrib><creatorcontrib>Jin, Xiaohong</creatorcontrib><creatorcontrib>Wu, Jinglan</creatorcontrib><creatorcontrib>Qian, Wenbin</creatorcontrib><creatorcontrib>Liu, Dong</creatorcontrib><creatorcontrib>Xie, Jingjing</creatorcontrib><creatorcontrib>Bai, Jianxin</creatorcontrib><creatorcontrib>Ying, Hanjie</creatorcontrib><title>Optimization and Validation of a GC-FID Method for the Determination of Acetone-Butanol-Ethanol Fermentation Products</title><title>Journal of chromatographic science</title><addtitle>J Chromatogr Sci</addtitle><description>An improved, simple gas chromatography-flame ionization detection (GC-FID) method was developed for measuring the products of acetone-butanol-ethanol (ABE) fermentation and the combined fermentation/separation processes. The analysis time per sample was reduced to less than 10 min compared to those of a conventional GC-FID (more than 20 min). The behavior of the compounds in temperature-programmed gas chromatographic runs was predicted using thermodynamic parameters derived from isothermal runs. The optimum temperature programming condition was achieved when the resolution for each peak met the analytical requirement and the analysis time was shortest. With the exception of acetic acid, the detection limits of the presented method for various products were below 10 mg/L. The repeatability and intermediate precision of the method were less than 10% (relative standard deviation). Validation and quantification results demonstrated that this method is a sensitive, reliable and fast alternative for conventional investigation of the adsorption-coupled ABE fermentation process.</description><subject>Acetone - analysis</subject><subject>Acetone - chemistry</subject><subject>Acetone - isolation & purification</subject><subject>Acetone - metabolism</subject><subject>Butanols - analysis</subject><subject>Butanols - chemistry</subject><subject>Butanols - isolation & purification</subject><subject>Butanols - metabolism</subject><subject>Chromatography, Gas - methods</subject><subject>Clostridium acetobutylicum - metabolism</subject><subject>Ethanol - analysis</subject><subject>Ethanol - chemistry</subject><subject>Ethanol - isolation & purification</subject><subject>Ethanol - metabolism</subject><subject>Fermentation</subject><subject>Limit of Detection</subject><subject>Reproducibility of Results</subject><issn>0021-9665</issn><issn>1945-239X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0DtPwzAUBWALgWgpzGzII0IK9StJPZa-qFRUBkBskeOHGpTEwXYG-PWkStuV6epI3z3DAeAWo0eMOB3LnbOVl8U4rwIi5AwMMWdxRCj_PAdDhAiOeJLEA3Dl_dc-4kl8CQaExoQjlg5Bu21CURW_IhS2hqJW8EOUheqjNVDA1SxarufwRYedVdBYB8NOw7kO2lVFfYJTqYOtdfTUBlHbMlqE3f7CZcd0HXr36qxqZfDX4MKI0uubwx2B9-XibfYcbbar9Wy6iSRFNESMJwLLWFGWS0YoYkpNmCG5ik2SsHQiaSpSrajBJpecJBJxqVRKRJwaTFRKR-C-722c_W61D1lVeKnLUtTatj7DjHNMWMJoR8c9lc5677TJGldUwv1kGGX7rbPj1lm_dfdxdyhv80qrkz-O24GHHti2-bftD_vZjU0</recordid><startdate>201403</startdate><enddate>201403</enddate><creator>Lin, Xiaoqing</creator><creator>Fan, Jiansheng</creator><creator>Wen, Qingshi</creator><creator>Li, Renjie</creator><creator>Jin, Xiaohong</creator><creator>Wu, Jinglan</creator><creator>Qian, Wenbin</creator><creator>Liu, Dong</creator><creator>Xie, Jingjing</creator><creator>Bai, Jianxin</creator><creator>Ying, Hanjie</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201403</creationdate><title>Optimization and Validation of a GC-FID Method for the Determination of Acetone-Butanol-Ethanol Fermentation Products</title><author>Lin, Xiaoqing ; Fan, Jiansheng ; Wen, Qingshi ; Li, Renjie ; Jin, Xiaohong ; Wu, Jinglan ; Qian, Wenbin ; Liu, Dong ; Xie, Jingjing ; Bai, Jianxin ; Ying, Hanjie</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c303t-496a1c5d34bc42304dd84f2bd5f66478c37a7ed3f1fbc926c09cdd72a57f12d73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Acetone - analysis</topic><topic>Acetone - chemistry</topic><topic>Acetone - isolation & purification</topic><topic>Acetone - metabolism</topic><topic>Butanols - analysis</topic><topic>Butanols - chemistry</topic><topic>Butanols - isolation & purification</topic><topic>Butanols - metabolism</topic><topic>Chromatography, Gas - methods</topic><topic>Clostridium acetobutylicum - metabolism</topic><topic>Ethanol - analysis</topic><topic>Ethanol - chemistry</topic><topic>Ethanol - isolation & purification</topic><topic>Ethanol - metabolism</topic><topic>Fermentation</topic><topic>Limit of Detection</topic><topic>Reproducibility of Results</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lin, Xiaoqing</creatorcontrib><creatorcontrib>Fan, Jiansheng</creatorcontrib><creatorcontrib>Wen, Qingshi</creatorcontrib><creatorcontrib>Li, Renjie</creatorcontrib><creatorcontrib>Jin, Xiaohong</creatorcontrib><creatorcontrib>Wu, Jinglan</creatorcontrib><creatorcontrib>Qian, Wenbin</creatorcontrib><creatorcontrib>Liu, Dong</creatorcontrib><creatorcontrib>Xie, Jingjing</creatorcontrib><creatorcontrib>Bai, Jianxin</creatorcontrib><creatorcontrib>Ying, Hanjie</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of chromatographic science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lin, Xiaoqing</au><au>Fan, Jiansheng</au><au>Wen, Qingshi</au><au>Li, Renjie</au><au>Jin, Xiaohong</au><au>Wu, Jinglan</au><au>Qian, Wenbin</au><au>Liu, Dong</au><au>Xie, Jingjing</au><au>Bai, Jianxin</au><au>Ying, Hanjie</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Optimization and Validation of a GC-FID Method for the Determination of Acetone-Butanol-Ethanol Fermentation Products</atitle><jtitle>Journal of chromatographic science</jtitle><addtitle>J Chromatogr Sci</addtitle><date>2014-03</date><risdate>2014</risdate><volume>52</volume><issue>3</issue><spage>264</spage><epage>270</epage><pages>264-270</pages><issn>0021-9665</issn><eissn>1945-239X</eissn><abstract>An improved, simple gas chromatography-flame ionization detection (GC-FID) method was developed for measuring the products of acetone-butanol-ethanol (ABE) fermentation and the combined fermentation/separation processes. The analysis time per sample was reduced to less than 10 min compared to those of a conventional GC-FID (more than 20 min). The behavior of the compounds in temperature-programmed gas chromatographic runs was predicted using thermodynamic parameters derived from isothermal runs. The optimum temperature programming condition was achieved when the resolution for each peak met the analytical requirement and the analysis time was shortest. With the exception of acetic acid, the detection limits of the presented method for various products were below 10 mg/L. The repeatability and intermediate precision of the method were less than 10% (relative standard deviation). Validation and quantification results demonstrated that this method is a sensitive, reliable and fast alternative for conventional investigation of the adsorption-coupled ABE fermentation process.</abstract><cop>United States</cop><pub>Oxford University Press</pub><pmid>23529047</pmid><doi>10.1093/chromsci/bmt022</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Acetone - analysis Acetone - chemistry Acetone - isolation & purification Acetone - metabolism Butanols - analysis Butanols - chemistry Butanols - isolation & purification Butanols - metabolism Chromatography, Gas - methods Clostridium acetobutylicum - metabolism Ethanol - analysis Ethanol - chemistry Ethanol - isolation & purification Ethanol - metabolism Fermentation Limit of Detection Reproducibility of Results |
| title | Optimization and Validation of a GC-FID Method for the Determination of Acetone-Butanol-Ethanol Fermentation Products |
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