Direct in vitro selection of titanium-binding epidermal growth factor

Abstract Epidermal growth factor (EGF) with affinity to TiO2 surfaces was obtained by direct in vitro selection. A random peptide library was generated for fusion to the N-terminal of EGF, and polypeptides exhibiting affinity were selected in vitro by ribosome display. The best-performing polypeptid...

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Veröffentlicht in:	Biomaterials 2014-04, Vol.35 (11), p.3497-3503
Hauptverfasser:	Tada, Seiichi, Timucin, Emel, Kitajima, Takashi, Sezerman, Osman U, Ito, Yoshihiro
Format:	Artikel
Sprache:	eng
Schlagworte:	Advanced Basic Science Affinity Amino Acid Sequence Animals Cell Proliferation - drug effects Crystallography, X-Ray Cytokine Dentistry Epidermal Growth Factor - chemistry Epidermal Growth Factor - metabolism Epidermal Growth Factor - pharmacology HeLa Cells Humans Mice Molecular Dynamics Simulation Molecular Sequence Data NIH 3T3 Cells Peptide Peptides - chemistry Peptides - metabolism Photoelectron Spectroscopy Protein Binding - drug effects Structural Homology, Protein Surface modification Titanium Titanium - metabolism
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